网络教育资源构建中Windows Media流媒体技术的应用与研究

Windows Media流媒体由于交互性强、使用方便和效率高，使得在网络教育中的应用逐渐增多。本文讨论了Windows Media流媒体技术在网络教育和资源构建中应用的优势及其在设计、制作、发布、应用等各环节的相关理论和技术细节。     

烧伤后早期应用中/长链甘油三酯对患者免疫功能的影响

目的　探讨烧伤后早期肠内喂养中链甘油三酯 (MCT) /长链甘油三酯 (LCT)对机体免疫功能的影响及其可能机制。　方法　选取 30例烧伤面积 >30 %TBSA的患者 ,随机分为两组 (每组 15例 )。F组 ,饲以含MCT/LCT的肠内营养制剂Fresubin 75 0MCT ;N组 ,饲以只含LCT的肠内营养制剂Nutrison。于伤后 2 4h内进行完全肠内营养支持 ,共持续 10d。于伤后 1、4、7、10d检测两组患者血浆白细胞介素 (IL) 2、IL 4、前列腺素 (PG)E2 及外周血T淋巴细胞转化率的改变。　结果　伤后各时相点F组患者血浆IL 2水平与N组相比 ,无明显差异 (P >0 .0 5 ) ;伤后 4dF组PGE2 水平较N组明显降低 (P <0 .0 1) ;伤后 4、7、10dF组IL 4水平及外周血T淋巴细胞转化率均明显高于N组 (P <0 .0 5～ 0 .0 1)。　结论　在改善烧伤后机体的免疫功能方面 ,含MCT/LCT的肠内营养制剂较单纯LCT制剂更具优势。     

三联药物治疗未破裂异位妊娠的临床研究

目的 :观察甲氨蝶呤(Methotrexate,MTX)、米非司酮(Mifepristron,Ru486)及中药三联用药治疗未破裂型异位妊娠的临床疗效。方法 :对171例未破裂型异位妊娠病人 ,按药物治疗方法不同分为两组进行对照研究 :A组使用MTX注射 +Ru486口服 +中药联合治疗。B组单独使用MTX注射治疗。测定法定期测定两组病人血HCG值以监测血HCG下降情况 ,测量异位妊娠包块三径线值以监测病灶包块缩小情况 ,同时观察临床症状持续时间、副反应发生率及总有效率。结果 :血HCG下降及病灶包块缩小A组较B组明显 ,差异有显著性(p<0 05,p<0 01);临床症状持续时间A组较B组短 ,差异有显著性(p<0 05);副反应发生率两组间无显著差异(p>0 05);A、B两组的总有效率分别为 :95 74%和71 43% ,A组较B组高 ,差异有显著性(p<0 05)。结论 :三联使用甲氨蝶呤、米非司酮及活血化瘀消包块杀胚的中药既有很强的杀胚功能 ,又能消除病灶包块 ,保留生育功能。三联药物联合治疗未破裂异位妊娠具有疗效肯定、安全     

21 d-6°头低位卧床期间运动训练对动态姿态平衡的影响

目的探讨21 d-6°头低位卧床和卧床运动训练对动态姿态平衡和控制功能的影响. 方法 10名健康男性青年被试者分为卧床对照组和卧床训练组,每组5人,卧床期间训练组每天进行2次、每次30 min逐级增加负荷的头低位功率自行车训练;分别于卧床前和卧床后21 d进行了动态姿态平衡和双膝等速肌力的测试. 结果与卧床前相比,对照组动态本体感觉得分和运动控制适应能力明显降低,双膝相对峰力矩明显降低,同时伴有腘绳肌与股四头肌峰力矩屈/伸比值的明显增加;训练组动态本体感觉得分和运动控制适应能力明显高于对照组,而腘绳肌与股四头肌峰力矩屈/伸比值无明显改变. 结论 21 d-6°头低位卧床运动训练能够明显改善卧床后动态姿态平衡及其动态运动适应功能.     

单纯下颌骨磨削法矫治下颌角肥大

目的 探讨下颌角肥大骨磨削法治疗的临床效果。方法 在局部肿胀麻醉下，采用口内切口入路，常规分离显露下颌骨，在预定需要矫正的下颌骨区域，使用特殊器械磨削下颌骨体外侧皮质骨、下颌骨下缘及下颌角骨质，同时对部分肥大的咬肌进行处理，矫治下颌角肥大。结果 对58例下颌角肥大患者均行下颌骨磨削法矫治，除1例术中损伤下齿槽血管引起出血外，无其他并发症发生。术后随访6～18个月，全部病例取得良好的矫治效果。结论 下颌骨磨削法矫治下颌角肥大是一种并发症少、安全有效的好方法。与截骨矫治下颌角肥大的方法相比，该术式保留了下颌骨的内板。维持了颌颈区的立体感。     

Urinary medium-chain acylcarnitines in medium-chain acyl-CoA dehydrogenase deficiency, medium-chain triglyceride feeding and valproic acid therapy: sensitivity and specificity of the radioisotopic exchange/high performance liquid chromatography method.

To determine the sensitivity and specificity of detecting urinary medium-chain acylcarnitines for the diagnosis of MCAD deficiency, 114 urine specimens from 75 children with metabolic diseases and controls were analyzed in a blinded fashion using a radioisotopic exchange/HPLC method. All 47 patients with MCAD deficiency were correctly diagnosed using the criterion hexanoylcarnitine or octanoylcarnitine peak areas larger than those of other medium-chain acylcarnitines. The majority of them were tested during the asymptomatic state without L-carnitine loading. Four patients with other defects of fatty acid oxidation and three patients receiving valproic acid had a similar acylcarnitine excretion pattern. To further examine the specificity of the method, eight infants receiving a diet enriched with medium-chain triglycerides and 13 additional patients receiving valproic acid were studied. Most of these also tested positive for MCAD deficiency by the above criterion. Analysis by a new gas chromatographic-mass spectrometric procedure revealed that octanoylcarnitine, not valproylcarnitine, was the most abundant medium-chain carnitine ester excreted by a patient treated with valproic acid. Quantitation of urinary hexanoylcarnitine and octanoylcarnitine showed considerable overlap among patients with MCAD deficiency and those receiving valproic acid or a medium-chain triglyceride-enriched diet. MCAD deficiency can be reliably detected in urine specimens by this method without the need for prior carnitine loading. However, other defects in fatty acid oxidation must be differentiated from MCAD deficiency, and a history of medium-chain triglyceride or valproic acid administration must be considered if the diagnosis of MCAD deficiency is sought through analysis of urinary acylcarnitines.     

1株诱变枯草杆菌溶栓酶性质研究的初探

目的 对从1株诱变的枯草杆菌的发酵液中，通过分离纯化得到的一种活性较高的纤溶酶进行性质研究。方法 按胰酶活性测定法测其水解酪蛋白活性，用SDS-PAGE测定分子量，等电聚焦测定其等电点。采用硫酸酚法测定糖含量，并对其药理性质进行研究。结果 其水解酪蛋白活性为2.1U/mg，分子量为62kDa。等电点为8.5。含糖量为32%，并且发现该酶具有直接降解纤维蛋白的活性。结论 此纤溶酶具有较高的活性及药理价值。     

自由基损伤和β2糖蛋白1在自身免疫疾病中的作用

目的 研究被自由基氧化修饰的β2糖蛋白1(β2GPl)在自身免疫疾病中自身抗体产生中的作用。方法 采用高氯酸沉淀，两步肝素连接的交联琼脂糖凝胶层析结合葡聚糖凝胶G—100分离纯化大鼠β2GP1；应用自由基氧化修饰大鼠β2GP1；氧化修饰的β2GP1(oxβ2GPl)、β2GPl分别加与不加心磷脂(CL)及CL免疫大鼠，ELISA方法检测免疫大鼠血清中抗β2GP1抗体和抗心磷脂抗体(ACA)。结果 两步亲和层析结合凝胶过滤层析有效地分离纯化了大鼠β2GPl，蛋白纯度达90．78％；通过碳基含量测定证实了自由基对β2GP1的氧化修饰；oxβ2GGP1加CL、β2GP1加CL、oxβ2GPl免疫组与空白对照组、实验对照组比较ACA水平有显著升高(P＜0.001)，oxβ2GP1加CL免疫组与β2GPl加CL免疫组比较ACA水平差异有显著性(P＜0.05)；oxβ2GP1免疫组与oxβ2GPl加CL免疫组比较ACA水平差异不显著(P＞0.05)；oxβ2GPl加CL、oxβ2GPl免疫组与对照组比较，抗oxβ2GP1抗体水平有显著升高(P＜0.05)。结论 两步肝素连接的交联琼脂糖凝胶层析结合葡聚糖凝胶G—100能有效地分离纯化β2GPl；oxβ2GPl诱导了ACA和抗oxβ2GPl抗体的产生；β2GPl可能是自身免疫疾病发生过程重要的辅助因子或靶抗原，而自由基对β2GP1损伤可能是自身免疫疾病发病机制中的一个重要病理过程。     

首例中国可视化人体心脏三维重建及临床意义

目的 对首例中国数字化可视人体的心脏进行三维重建，为心脏疾病的影像学诊断和外科手术提供三维解剖学依据。方法 将首例中国数字化可视人体心脏部分的薄层断面(层厚1．0mm)输入SGI工作站，经数据分割、对位重建、平滑处理和三维显示等步骤，完成对心脏结构的三维重建。结果 本研究完整地重建出了心脏结构，重建的结构能够以多结构、多彩色实体模型方式显示，可显示心脏内部各结构的空间位置和毗邻关系，可在三维空间伴置上绕任意轴旋转任意角度。结论 重建出的三维图像清晰地显示了心脏内部和整体的结构，实现了心脏的二维可视化。     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.