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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
目的:动态观察大鼠C6胶质瘤模型的自然生长过程及反义IGF-1mRNA对胶质瘤的治疗效果和治疗机制。方法:将32只Wistar大鼠分成4组,每组8只,(1)对照组(C组)于右尾状核接种C6鼠胶质瘤细胞。(2)治疗组(T组):于右尾状核接种C6细胞后的第7、10天在接种点注射脂质体包裹的质粒P-anti-IGF-1,对胶质瘤进行治疗于右尾状核接种经反义IGF-1mRNA转染的C6胶质瘤细胞。(4)K 相似文献
992.
采用体外培养人淋巴细胞微核检测法,探讨复方云芝胶囊抗突变作用。结果表明,复方云芝胶囊(10mg/L~50mg/L)可显著抑制丝裂霉素C(MMC)诱发的微核形成,并可拮抗老年人自发微核,增加细胞增殖活性。 相似文献
993.
用LC/MS/MS和RP—HPLC法对泻心汤煎煮产生的沉淀物的研究 总被引:3,自引:0,他引:3
目的:用LC/MS/MS和RP-HPLC法研究沔心汤煎煮产生的沉淀物。方法:利用LC-MS-MS法对淀心肠沉淀物进行了初步研究,又利用RP-HPLC法,测定了淀心汤沉淀物中小檗碱在小鼠体内的吸收。结果:发现其中含有小檗碱、巴马丁碱、黄连碱、药根碱,但在给小鼠灌服淀心汤沉淀物后24h内的几个时间段中,均未能从血浆中测到小檗碱。结论:淀心汤沉淀物进入体内后小檗碱并未进入血液,推测如果淀心汤沉淀物具有恬 相似文献
994.
Smith Geoff Shekunov Boris Yu. Shen Jie Duffy Alistair P. Anwar Jamshed Wakerly Mark G. Chakrabarti Roma 《Pharmaceutical research》1996,13(8):1181-1185
Purpose. A knowledge of the interfacial properties of lecithin underpins our understanding of many of the physicochemical characteristics of drug delivery systems such as liposomes and lecithin stabilized microemulsions. In order to further this understanding, a high frequency dielectric study of the interfacial properties of egg lecithin liposomes was undertaken.
Methods. The effect of temperature, lecithin concentration and probe sonication on the interfacial dielectric properties of liposomal suspensions was investigated by high frequency dielectric relaxation spectroscopy between 0.2–6 GHz.
Results. The frequency dependent permittivity of each suspension exhibited a dielectric dispersion centred around 100 MHz, corresponding to the relaxation of zwitterionic head groups. The activation energy for head group reorientation was estimated as H = 6.3 kJ mol–1. There was an increase in extent of inter-head group interactions on increasing the liposome volume fraction, whereas the effect of probe sonication showed that: (i) head groups in both the outer and inner lamellae contribute to the dielectric response; (ii) the head groups may be less restricted in liposomes of high surface curvature with few lamellae; (iii) the high frequency permittivity of the suspension increased on sonication, as a result of a reduction in the amount of (depolarized) interlamellar water following a reduction in the number of lamellae per liposome.
Conclusions. Dielectric analysis of the zwitterionic head groups of lecithin therefore provides a means for investigating the surface of lecithin liposomes, and may be used to investigate the effect of drugs and other solutes on membranes. 相似文献
995.
Newton D Pearson J Xue Y Smith M Fogler W Mikulski S Alvord W Kung H Long D Rybak S 《International journal of oncology》1996,8(6):1095-1104
Onconase, a ribonuclease isolated from Rana pipiens oocytes and early embryos, is a member of the RNase A superfamily. Onconase has anti-neoplastic properties both in vitro and in vivo, and is undergoing clinical evaluation. In the present study, Onconase was combined with or conjugated to MRK16, an anti-P-glycoprotein (Pgp) monoclonal antibody. The interaction of these combinations with vincristine (VCR) against parental and multidrug resistant (MDR), Pgp expressing, human colon carcinoma cells caused increased VCR cytotoxicity in vitro and enhanced survival of athymic nude mice given transplants of drug resistant HT-29(mdr1) cells in vivo. The results suggest that combination treatment with Onconase and other agents that modulate the chemosensitivity of Pgp-expressing human tumor cells has the potential to overcome MDR. 相似文献
996.
997.
Piotr Kruszewski Maria Luisa Fasano AIf O. Brubakk Jie Ming Shen Trond Sand Ottar Sjaastad 《Headache》1991,31(6):399-405
The recently described "Sunct" syndrome is a rare picture of unilateral, shortlasting headache attacks accompanied by autonomic phenomena (conjunctival injection, tearing, etc.) on the symptomatic side. Heart rate and blood pressure were monitored in two elderly "Sunct" patients during and outside headache attacks. An ultrasound Doppler servo method was used for the non-invasive, continuous, beat-to-beat determination of instantaneous arterial blood pressure. In a third patient, systolic and diastolic blood pressure, both outside and during pain paroxysms, were assessed using the standard Korotkoff method. Heart rate was found to be significantly decreased during pain paroxysms. Systolic blood pressure was observed to be significantly increased during attacks, when compared with the inter-attack period, while a less consistent pattern was observed for diastolic blood pressure. Some of the changes in the cardiovascular system seemed to start prior to pain onset. Therefore, it seems unlikely that these changes were caused by pain activation of the sympathetic nervous system or the oculocardiac reflex. 相似文献
998.
Phytate was digested by wheat bran phytase to yield inositol tetrakisphosphate. Periodate-oxidized inositol tetrakisphosphate (oxyIP4) was coupled by means of reductive alkylation to hemoglobin and the covalent dextran-hemoglobin conjugate to yield the rightshifted (rs) compounds rsHb and rsDxHb, respectively. The variations of the oxygen dissociation curves of these molecules with pH and temperature were compared to those of hemoglobin. The variations with pH were found to be less pronounced for these rightshifted forms. An extensive decrease in the half-saturation oxygen tension was observed, however, with both rsHb and rsDxHb, as in the case of unmodified Hb. Modification of hemoglobin by oxyIP4 at the polyphosphate site was suggested by the lack of a further rightshifting effect of phytate on rsHb, and by the similarity between the difference spectrum of rs-methemoglobin and the difference spectrum induced by the addition of phytate. 相似文献
999.
1000.
高效液相色谱法测定复方苯甲酸乳膏中苯甲酸和水杨酸的含量 总被引:3,自引:1,他引:3
目的:建立高效液相色谱法(HPLC)测定复方苯甲酸乳膏中苯甲酸和水杨酸的含量。方法:色谱柱为Hypersil C_(18)柱(4.6nm×250mm,5μm),流动相为甲醇-水(44:56),流速0.8mL·min~(-1),检测波长为226nm。结果:苯甲酸和水杨酸分别在15.20~76.00mg·L~(-1)和7.64~38.20mg·L~(-1)范围内线性良好(r=0.9999);日内、日间RSD在0.030~4.79%,高、中、低浓度回收率在98.54%~101.20%。分析了3批样品,结果满意。结论:本方法简便,快速,准确,可作为复方苯甲酸乳膏的质量控制方法。 相似文献