Association of the GNAS1 gene variant with hypertension is dependent on alcohol consumption.

The beta-adrenoceptor (beta-AR)-stimulatory guanine nucleotide-binding (Gs) protein system has been shown to play important roles in the cardiovascular system. The gene encoding the alpha-subunit of Gs proteins (GNAS1) is a candidate genetic determinant for hypertension. Because alcohol consumption is known to affect blood pressure partly through the beta-AR-Gs protein system, we examined the possible interaction between GNAS1 T393C polymorphism and drinking status in the association with hypertension in the present study. As a result, a non-significant but reasonable trend supporting the presence of an interaction was shown (p = 0.076). In line with this trend, the T393C polymorphism significantly interacted with drinking status in the association with systolic blood pressure (p = 0.028). Moreover, supporting the presence of an interaction, T allele carriers consistently had a higher probability of hypertension, higher systolic blood pressure, and higher diastolic blood pressure than CC homozygotes in non-drinkers and light drinkers. In contrast, CC homozygotes consistently had a higher probability of hypertension, higher systolic blood pressure, and higher diastolic blood pressure than T allele carriers in moderate to heavy drinkers. The present study also showed a significant interaction between the T393C polymorphism and drinking status in the association with pulse pressure (p = 0.026), reflected by a significant association between the T393C polymorphism and pulse pressure in moderate to heavy drinkers (p = 0.026). These findings may be helpful in conducting further molecular and biological studies on the relationship among the effects of alcohol, the beta-AR-Gs protein system, and hypertension.     

SPECT探头系统的校正与调试

本文阐明了对SPECT探头校正与调试的重要性,特别是对探头校正与调试的具体方法与步骤作了详细介绍.     

调气活血汤治疗崩漏34例

崩漏是月经周期、经期、经量严重失常的病证，是指经血非时暴下不止或淋漓不尽而言。调气活血汤为韩百灵氏治疗崩漏之经验方，韩氏认为本方证多由情志不遂，积思郁怒，或经产后，余血未尽，感寒涉水，过食生冷，不禁房事，余血停滞，瘀阻冲任，心血不得归经，而致崩中漏下。临证时须审因论治，若气病及血者，以活血为主，调气为辅。笔者自2005年以来，运用本方治疗妇女崩漏病34例，取得满意疗效，现报道如下。     

小切口胆囊切除术108例临床观察

本文报告腹部切口５－８ｃｍ的小切口胆囊切除术１０８例，与同期大切口胆囊切除术相比，具有创伤较小、恢复较快、并发症少、切口疤痕小的优点，虽不如腹腔镜胆囊切除术（ＬＣ）的疼痛轻、恢复快，但并症比ＬＣ少，只要适应症选择得当，在术者的经验和技术较成熟的情况下，不昔为一种可供选择的方法。     

紫外分光光度法测定SOD活性的应用及其意义

本文对SOD的紫外分光光度法测定进行了实用性研究。结果表明,本文方法具有用血量少,重现性好,快速、灵敏,试剂廉价等优点。西宁地区与平原地区人群的比较测定显示,前者SOD活性明显高于后者(P<0.01)。     

Characteristics of presystolic flow in the superior vena cava: new thoughts on a forgotten sound.

Cross sectional, M mode, and Doppler echocardiography, apexcardiography, and phonocardiography were used to characterise presystolic cardiovascular sounds in three patients with ventricular disease. Although the aetiology was different (dilated cardiomyopathy, primary pulmonary hypertension, and chronic pulmonary thromboembolic disease), in each case the presystolic sound was associated with a rapid change in acceleration of blood and with flow reversal in the superior vena cava, and could only be recorded at the right sternal edge or over the jugular veins. Such flow characteristics may be explained by a raised ventricular end diastolic pressure with reduced compliance. Use of these techniques helps to understand the cause of a previously described but little recognised heart sound, and adds weight to the interpretation of its presence in disease.     

精氨酸加压素（AVP）的衍生物AVP4—8不能作用于AVPV1a受体而诱发电流反应

在微量注射大量肝脏ｍＲＮＡ之后，通过电压箝方法进行功能鉴定，两栖类卵母细胞成功地表达了ＡＶＰＶ１ａ受体。但在灌流ＡＶ４－８溶液时，却不能诱导卵母细胞产生内向振荡电流反应。提示ＡＶＰ４－８不能通过ＡＶＰＶ１ａ受体而介导生理学效应。     

Location of lectin UEA-I receptors in histological diagnosis of nasopharyngeal precancerous lesion.

Three kinds of lectins (UEA-I, ConA, PNA) were used to study normal, dysplastic, neoplastic nasopharyngeal epithelium by lectin affinitive histochemical method. UEA-I (ulex europeus agglutinin-I) displayed membrane distribution in normal squamous epithelium, but most of nasopharyngeal carcinoma cells were negative. Notably, severe dysplastic epithelium (precancerous lesion) exhibited a strong membranous and cytoplasmic affinity, which contrasted sharply with the normal epithelium and carcinoma cells. The statistically significant difference in the content and distribution of lectin UEA-I in these three groups suggest that UEA-I is a hopeful marker for diagnosing precancerous lesion of the nasopharynx. However, PNA and ConA are of less diagnostic value.

     

HMME在不同溶液中光漂白反应动力学规律

目的 分析国产光敏剂血卟啉单甲醚（HMME）在不同溶液中光漂白反应动力学规律，加深对光漂白反应复杂反应过程的理解，并为HMME-PDT治疗微血管病变的数学建模研究提供实验依据和参数。 方法 根据光化学反应动力学原理，从理论上推导出在单纯溶液和复杂溶液（含可被光氧化的底物）中，单态氧介导的光漂白反应动力学方程，通过对实验测定的漂白数据（不同时间点的HMME浓度）进行拟合，进一步验证理论推导出光漂白反应动力学方程。 结果 HMME在单一溶液体系（PBS、DMSO）中的光漂白符合1级反应动力学过程，在含底物的复杂体系（白蛋白缓冲液、细胞悬液）中符合2级反应动力学过程。 结论 HMME在不同溶液体系中的光漂白遵循不同的反应动力学规律。根据化学反应动力学原理进行光漂白等复杂光化学反应过程的数学模拟是可行的，并有利于对复杂光化学反应过程的深入理解。