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991.
Effects of Mitofusin-2 Gene on Cell Proliferation and Chemotherapy Sensitivity of MCF-7 总被引:1,自引:0,他引:1
In order to evaluate the effect of mitofusin-2 gene (mfn2) on proliferation and chemotherapy sensitivity of human breast carcinoma cell line MCF-7 in vitro, pEGFPmfn2 plasmid carrying full length of mitofusin-2 gene was transfected, by using sofast, into MCF-7 cells. Mitofusin-2 gene expression in MCF-7 cells transfected by sofast after 48 h was detected by PCR and Western blotting, and the stable expression of GFP protein in MCF-7 cells by Western blot analysis. The proliferation of MCF-7 cells was assayed by MTT and cell counting. By using PI method, the effects of mfn2 on the cell cycle distribution of MCF-7 were measured. Annexin-Ⅴ/PI double labeling method was employed to detect the changes in apoptosis induced by chemotherapeutics before and after transfection. The results showed that the MCF-7 cells transfected with mfn2 gene could stably and highly express GFP protein. MTT assay revealed that after transfection of mfn2 cDNA, the proliferation of MCF-7 cells was significantly inhibited. DNA histogram showed that cells arrested in S phase, and the percentage of S phase cells was 42.7, 17.2 and 19.6 in mfn2 cDNA transfection group, blank plasmid transfection group and blank control group, respectively (P〈0.05). The apoptosis ratio of the cells transfected with mfn2 gene was increased from 3.56% to 15.95%, that of the cells treated with camptothecin (CAMP) followed by mfn2 gene transfection was 69.6%, and that in blank plasmid transfection group and blank control group was 31.0% and 23.4% respectively (P〈0.05). It was suggested that transfection of mfn2 gene could significantly inhibit the proliferation of MCF-7 cells and promote their sensitivity to CAMP with a synergic effect. 相似文献
992.
Li Qianning Yang Yimin Ying Dajun Cheng Rongchuan Gong Zili Liu Yong Zhou Zhujuan Zheng Jian 《中国人民解放军军医大学学报》2008,23(3):154-161
Objective: To determine the effect of antiparallel phosphorothioate triplex-forming oligonucleotide (apsTFO), which was designed according to shear stress response element (SSRE) in tissue factor (TF) gene promoter region, on the expression of endothelial TF in carotid artery stenosis rats. Methods: Rat model of severe carotid artery stenosis were inflicted by silica gel tube ligation. Half an hour before the model infliction, GT20-apsTFO, GT20-psTFO and GT21-apsTFO labeled with green fluorescence (FITC) were injected into the vena caudalis of rat at a dose of 0.5 mg/kg. Half an hour, 4 or 9 h after the ligation, the distribution of TFO in the common carotid artery, the liver and the kidney was detected with aid of fluorescence microscopy. And the mRNA and protein expressions of TF, Egr-1 and Spl in the above-mentioned organs were determined with in situ hybridization and immunohistochemical assay respectively in 6 h after the model establishment, and the results were analyzed with an image analysis system. Results: Only in 1 h after TFO injection, fluorescent granules appeared in the liver, the kidney and the vascular wall and lumen of carotid artery, and then in 4.5 h, they still deposited in above sites except the vascular lumen. GT20-apsTFO and GT21-apsTFO significant down-regulated the mRNA and protein expressions of TF compared to the rats without treatment (P〈0.05), and the former apsTFO had a more stronger effect than the later (P〈0.05). GT20-psTFO had no such effect (P〉0.05). The 3 TFOs had no inhibition on the mRNA and protein expressions of Egr-1 and Spl. Conclusion: Pretreated apsTFO can partly come into the vascular endothelial cells, and inhibit TF expression induced by shear stress, but had no effect on Egr-1 and Spl gene expressions. 相似文献
993.
目的 探讨老年高血压病患者静息心率(resting heart rate,RHR)与早期颈动脉硬化的关系.方法 入选老年高血压病患者200例,按收缩压(SBP)水平分为三组:SBP<160mmHg,SBP 160~180mmHg,SBP>180mmHg.在以上每一组中,又按RHR水平分为:RHR1组<65次/min;RHR2组65~69次/min;RHR3组70~74次/min;RHR4组75~79次/min;RHR5组≥80次/min.对入选患者做超声检查颈动脉内膜-中膜厚度(intimal medial thickness,IMT)、颈总动脉分叉处内径(CAD).并测空度血糖,血脂.结果 RHR5组或RHR4组的颈动脉内膜厚度和颈动脉内径大于RHR1和RHR2组.结论 RHR的增加是老年高血压病患者颈动脉硬化的重要危险因素. 相似文献
994.
The remodeling process of synapses and neurotransmitter receptors of facial nucleus were observed. Models were set up by facial-facial anastomosis in rat. At post-surgery day (PSD) 0,7,21and 60, synaptophysin (p38), NMDA receptor subunit 2A and AMPA receptor subunit 2 (GluR2) were observed by immunohistochemical method and semi-quantitative RT-PCR, respectively. Mean-while, the synaptic structure of the facial motorneurons was observed under a transmission electron microscope (TEM). The intensity of p38 immunoreactivity was decreased, reaching the lowest value at PSD day 7, and then increased slightly at PSD 21. Ultrastructurally, the number of synapses in nucleus of the operational side decreased, which was consistent with the change in P38 immunoreactivity. NMDAR2A mRNA was down-regulated significantly in facial nucleus after the operation (P〈0.05), whereas AMPAR2 mRNA levels remained unchanged (P〉0.05). The synapses innervation and the expression of NMDAR2A and AMPAR2 mRNA in facial nucleus might be modified to suit for the new motor tasks following facial-facial anastomosis, and influenced facial nerve regeneration and recovery. 相似文献
995.
目的:分析及探讨近10年来我院围生儿死亡原因的分类现状,以提高围生保健工作质量,降低围生儿死亡率。方法:按照我国围生期分类标准,对我院10年间394例围生儿死亡病历进行统计分析。结果:10年间我院围生儿死亡率为2.28%,死胎占55.33%,死产占8.38%,新生儿死亡占36.29%。早产、畸形、脐带、胎盘因素是造成我院围生儿死亡的主要原因。结论:加强孕期监护,产前筛查,预防早产,防止并发症发生,是降低围生儿死亡的主要措施。 相似文献
996.
997.
目的了解北京市血液安全及执法监督现状,为做好血液安全执法监督工作提供参考依据。对象和方法北京市10家血站;从事血液安全执法71名监督员;采用分层抽样方法随机抽取北京市涉及临床用血的31家医疗机构家。结论在采供血方面,血站的设置规划、街头采血、血液运输方面不符合相关规定或存在血液安全隐患;在临床用血方面,输血科的建设、血液来源、临床输血与审批不符合相关规定或存在薄弱环节;在血液安全执法监督方面.监督职能尚未到位、监督能力有待提高。 相似文献
998.
新形势下的医院档案的管理 总被引:1,自引:0,他引:1
市场经济体制的建立和科学技术的迅猛发展,对医院档案管理现代化也提出了新的要求,这就需要我们进一步建立健全科学的档案管理体系,利用各种信息管理技术以及先进的设备和手段,不断提高医院档案工作水平,提高工作效率,从而促使档案资源更好地为医疗、教学、科研、管理服务。 相似文献
999.
目的:建立生活饮用水中微量钡的镧涂层石墨管原子吸收光谱测定方法.方法:以磷酸二氢铵作基体改进剂,对石墨管进行涂镧处理,用塞曼扣背景,石墨炉原子吸收法测定生活饮用水中微量钡的含量.结果:在0~50.0μ/L范围内具有良好的线性,相关系数为0.9996,方法的最低检出限为2.8×10-12g,加标回收率为92.0%~101.3%,RSD小于5.0%.结论:方法简便、准确,适于饮用水中微量钡的测定. 相似文献
1000.
前列腺素和γ干扰素对人黄体细胞甾体激素生成的影响及相互作用 总被引:4,自引:0,他引:4
我们以前的工作显示T-淋巴细胞产生的细胞因子γ干扰素(IFN-γ),可能在人黄体退化的过程中,通过抑制黄体孕酮生成而起作用。已知在大多数哺乳类动物中,前列腺素F2a(PGF2a)是一个重要的溶黄体因子。本工作对γ干扰素与前列腺素的关系作了进一步探讨。实验将人黄体细胞置于有或无γ干扰素的条件下培养4天,同时,测定孕酮、PGF2a、PGE2和6-酮-PGF1a的生成。伴随着γ干扰素对孕酮合成的抑制,观察到一个前列腺素合成的双相模式反应。即暴露于γ干扰素24h后,PGF2a和PGF1a的合成轻微降低,而培养96h后PGE2的合成增加。在另一实验中,发现PGE2和PGF2a对不同期的人黄体细胞在培养48h和96h后均有促黄体作用。此外,在消炎痛显著抑制PG合成的同时,γ干扰素仍能同样抑制其黄体细胞产生孕酮。这些结果提示:观察到γ干扰素引起的抑制孕酮产生似乎并非由前列腺素造成的,因为在同时加入可以抑制前列腺素合成的消炎痛时,这种抑制效果并不受影响。 相似文献