全文获取类型
收费全文 | 981155篇 |
免费 | 81802篇 |
国内免费 | 9876篇 |
专业分类
耳鼻咽喉 | 13764篇 |
儿科学 | 26112篇 |
妇产科学 | 27308篇 |
基础医学 | 138110篇 |
口腔科学 | 27428篇 |
临床医学 | 91168篇 |
内科学 | 183933篇 |
皮肤病学 | 18320篇 |
神经病学 | 76490篇 |
特种医学 | 39275篇 |
外国民族医学 | 241篇 |
外科学 | 148495篇 |
综合类 | 43233篇 |
现状与发展 | 26篇 |
一般理论 | 268篇 |
预防医学 | 74363篇 |
眼科学 | 24077篇 |
药学 | 76957篇 |
115篇 | |
中国医学 | 7559篇 |
肿瘤学 | 55591篇 |
出版年
2021年 | 10772篇 |
2019年 | 9197篇 |
2018年 | 11328篇 |
2017年 | 9366篇 |
2016年 | 9876篇 |
2015年 | 12796篇 |
2014年 | 17432篇 |
2013年 | 22652篇 |
2012年 | 31275篇 |
2011年 | 33273篇 |
2010年 | 19643篇 |
2009年 | 17642篇 |
2008年 | 28807篇 |
2007年 | 30821篇 |
2006年 | 30751篇 |
2005年 | 30398篇 |
2004年 | 27943篇 |
2003年 | 26847篇 |
2002年 | 25307篇 |
2001年 | 38570篇 |
2000年 | 39345篇 |
1999年 | 33968篇 |
1998年 | 11347篇 |
1997年 | 10627篇 |
1996年 | 9994篇 |
1995年 | 9427篇 |
1994年 | 8947篇 |
1992年 | 27729篇 |
1991年 | 27057篇 |
1990年 | 26435篇 |
1989年 | 25401篇 |
1988年 | 23838篇 |
1987年 | 23445篇 |
1986年 | 22175篇 |
1985年 | 21482篇 |
1984年 | 16600篇 |
1983年 | 14142篇 |
1982年 | 8952篇 |
1979年 | 16835篇 |
1978年 | 12162篇 |
1977年 | 10210篇 |
1976年 | 9362篇 |
1975年 | 10190篇 |
1974年 | 12693篇 |
1973年 | 12171篇 |
1972年 | 11554篇 |
1971年 | 10706篇 |
1970年 | 10230篇 |
1969年 | 9922篇 |
1968年 | 8907篇 |
排序方式: 共有10000条查询结果,搜索用时 312 毫秒
101.
本研究拟探讨染料木黄酮对前列腺癌细胞LNCaP和CWR22RV1的增殖、迁移和侵袭能力的影响。一、材料与方法1.材料:前列腺癌细胞株LNCaP和CWR22RV1(中国科学院上海生命科学研究院);Genistein(天津科瑞泰科技有限公司);RPMI 1640、胎牛血清(FBS)、胰蛋白酶(美国Gibco公司);E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin,Abcam公司);聚氰基丙烯酸正丁酯(BCA)试剂盒、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)上样缓冲液(×5)及蛋白质印迹法(Western blot)试剂盒(上海市碧云天生物公司);2.实验方法:采用噻唑蓝(MTT)实验、划痕实验和Transwell实验检测Genistein对细胞增殖、迁移及侵袭能力的影响。采用Western blot检测E-cadherin、N-cadherin、Vimentin、CD44和Oct-4的表达水平。 相似文献
102.
103.
Jae Eun Choi Tyler Werbel Zhenping Wang Chia Chi Wu Tony L. Yaksh Anna Di Nardo 《Journal of dermatological science》2019,93(1):58-64
Background
Rosacea is a chronic inflammatory skin condition whose etiology has been linked to mast cells and the antimicrobial peptide cathelicidin LL-37. Individuals with refractory disease have demonstrated clinical benefit with periodic injections of onabotulinum toxin, but the mechanism of action is unknown.Objectives
To investigate the molecular mechanism by which botulinum toxin improves rosacea lesions.Methods
Primary human and murine mast cells were pretreated with onabotulinum toxin A or B or control. Mast cell degranulation was evaluated by β-hexosaminidase activity. Expression of botulinum toxin receptor Sv2 was measured by qPCR. The presence of SNAP-25 and VAMP2 was established by immunofluorescence. In vivo rosacea model was established by intradermally injecting LL-37 with or without onabotulinum toxin A pretreatment. Mast cell degranulation was assessed in vivo by histologic counts. Rosacea biomarkers were analyzed by qPCR of mouse skin sections.Results
Onabotulinum toxin A and B inhibited compound 48/80-induced degranulation of both human and murine mast cells. Expression of Sv2 was established in mouse mast cells. Onabotulinum toxin A and B increased cleaved SNAP-25 and decreased VAMP2 staining in mast cells respectively. In mice, injection of onabotulinum toxin A significantly reduced LL-37-induced skin erythema, mast cell degranulation, and mRNA expression of rosacea biomarkers.Conclusions
These findings suggest that onabotulinum toxin reduces rosacea-associated skin inflammation by directly inhibiting mast cell degranulation. Periodic applications of onabotulinum toxin may be an effective therapy for refractory rosacea and deserves further study. 相似文献104.
105.
106.
Alan. B. Franklin Sarah N. Bevins Jeremy W. Ellis Ryan S. Miller Susan A. Shriner J. Jeffrey Root Daniel P. Walsh Thomas J. Deliberto 《Transboundary and Emerging Diseases》2019,66(2):705-714
Using data on waterfowl band recoveries, we identified spatially explicit hotspots of concentrated waterfowl movement to predict occurrence and spatial spread of a novel influenza A virus (clade 2.3.4.4) introduced from Asia by waterfowl from an initial outbreak in North America in November 2014. In response to the outbreak, the hotspots of waterfowl movement were used to help guide sampling for clade 2.3.4.4 viruses in waterfowl as an early warning for the US poultry industry during the outbreak . After surveillance sampling of waterfowl, we tested whether there was greater detection of clade 2.3.4.4 viruses inside hotspots. We found that hotspots defined using kernel density estimates of waterfowl band recoveries worked well in predicting areas with higher prevalence of the viruses in waterfowl. This approach exemplifies the value of ecological knowledge in predicting risk to agricultural security. 相似文献
107.
108.
Toshiro Hara Rony Chanoch-Myers Nathan D. Mathewson Chad Myskiw Lyla Atta Lillian Bussema Stephen W. Eichhorn Alissa C. Greenwald Gabriela S. Kinker Christopher Rodman L. Nicolas Gonzalez Castro Hiroaki Wakimoto Orit Rozenblatt-Rosen Xiaowei Zhuang Jean Fan Tony Hunter Inder M. Verma Kai W. Wucherpfennig Itay Tirosh 《Cancer cell》2021,39(6):779-792.e11
- Download : Download high-res image (228KB)
- Download : Download full-size image
109.
Stefaniuk Catherine M. Schlegelmilch June Meyerson Howard J. Harding Clifford V. Maitta Robert W. 《Journal of thrombosis and thrombolysis》2022,53(4):950-953
Journal of Thrombosis and Thrombolysis - Over the last few years data from our group have indicated that α-synuclein is important in development of immune cells as well as potentially... 相似文献
110.
Yinong Young-Xu Julia Thornton Snider Robertus van Aalst Salaheddin M. Mahmud Edward W. Thommes Jason K.H. Lee David P. Greenberg Ayman Chit 《Vaccine》2019,37(11):1484-1490