绝经前后妇女骨密度测定

目的研究妇女绝经后骨质疏松与雌激素的关系。方法收集了２５５例年龄在３２～７３岁之间的绝经前后的妇女。分成４组：月经正常、月经紊乱、自然绝经和人工绝经（双侧卵巢切除术）。用双能Ｘ线骨密度测定仪进行测量。结果妇女月经紊乱后,骨密度就明显下降,而绝经后的５年内显著下降。手术切除卵巢后,５年内骨密度也显著下降。结论绝经前切除卵巢或过早的闭经易导致骨质疏松,即妇女绝经后骨质疏松与雌激素下降有显著关系（Ｐ＜０．００１）。     

颈椎椎间盘与后部结构断层解剖及影像学研究

选用43具青年尸体,蛛网膜下腔注入铸型造影剂,低温冷冻,以椎间盘为中心制作断层标本,同时选取40例正常健康人进行超声、CT及MR扫描图像分析.发现同组中不同椎间盘水平测量数据的大小不同;解剖学与影像学各测量结果进行比较无显著性差异.     

Cold nerve injury is enhanced by intermittent cooling

Clinically nonfreezing cold nerve injury (NFCNI) is often preceded by fluctuating ambient temperatures. To simulate this we exposed the sciatic nerve of Wistar rats to intermittent cooling and rewarming. This paradigm was contrasted with one in which the sciatic nerve was continuously cooled. Despite an identical duration and degree of sciatic nerve cooling in both experiments, the pathology, as assessed neurophysiologically and morphologically, was much more severe when the nerve was intermittently cooled. It is likely that this exaggerated pathology is a result of repeated reperfusion injury. These experimental results suggest the need for a trial of free radical scavengers or antioxidants in models of NFCNI.     

体描法气道阻力测定分度判断矽肺阻塞性通气功能障碍的初步探讨

目的　探讨体描法气道阻力测定的分度值对矽肺阻塞性通气功能障碍进行分度。方法　选择进行过有关测定的矽肺患者２００ 例,以第１ 秒用力呼气量的分度值分为正常、轻度、中度、重度组观察各组体描法气道阻力测定项目的变化。结果　统计学处理各组间有显著性差异。２ 种测定法观察值有显著相关性,由此得体描法气道阻力各项目的分度值。结论　体描法气道阻力测定能直接反映气道阻力的变化且灵敏度高,人为因素较小,故用其分度值来判断矽肺阻塞性通气功能障碍可能更具有客观性     

自能源减速器称重动态过程的仿真研究

对自能源减速器称重过程的动态性能进行机理研究,建立了称重动态过程的数学模型,并进行了仿真计算,仿真结果与实测吻合.在此基础上分析了溜放速度、容腔及管路内油液的可压缩性对称重结果的影响.首次提出了"完全称重"、"部分称重"、"重复称重"的概念,为减速顶的设计提供新的理论依据.     

Immunohistochemical localization of dopamine-beta-hydroxylase in human and monkey eyes

PURPOSE: To investigate the pattern of dopamine-beta-hydroxylase (DBH)-containing fibers in human and monkey eyes. METHODS: DBH-containing fibers were detected by immunohistochemistry. The primary antibody used recognized DBH, the key enzyme in the conversion of dopamine to noradrenaline. RESULTS: In the anterior segment, DBH immunoreaction product was found in the peripheral corneal endothelium layer, in both the dilator and sphincter muscles of the iris, as well as in the anterior border layer of the iris. The ciliary muscle and the stroma of the ciliary processes were also zones of concentration. In the posterior segment, staining was seen around blood vessels in the choroid, in the vascular walls of the short posterior ciliary arteries and in the ciliary nerves. The retina was also immunopositive, with specific labeling in cones and rods of photoreceptors, inner and outer plexiform layers and ganglion cell layer. There was no significant difference in the distribution of DBH-related immunoreactivity in human and monkey eyes. CONCLUSIONS: The localization of DBH-related immunoreactivity is generally consistent with the known physiological roles of noradrenaline. However, an apparently high concentration of the enzyme in the anterior border layer of the iris and in retinal photoreceptors raises questions about the possible role of DBH-containing fibers in these structures.     

外伤角膜瘢痕无晶状体眼戴角膜接触镜后的角膜地形图改变

目的 探讨外伤性角膜瘢痕无晶状体眼戴硬性透气性接触镜前,后角膜表面的改变。方法 对戴该镜者１７眼及对照组（不戴镜）１２眼,在戴镜前后进行角膜地形图检测。结果 戴镜后平均８．４月,角膜散光减少（Ｐ〈０．０１）,角膜表面变得比较规则（Ｐ〈０．０５）,角膜表面的屈光度有变得相对比较平均的趋势。结论 戴硬性透气性角膜接触镜能使角膜瘢痕所致角膜散光减少,角膜表面变得较为规则。     

外伤性晶状体脱位继发性青光眼的治疗分析

目的 探讨外伤性晶状本脱位继发性青光眼的治疗方法。方法 药物保守治疗１８例,单纯小梁切除７例,单纯晶状体摘出１４例,晶状体摘出联合小梁切除７例。结果 ４６例中２例在局部滴用噻吗心安后,眼压控制在２１ｍｍＨｇ以下。治疗后矫正视力和前相比差异有显著性。结论 外伤性晶状体脱位继发性青光眼的治疗关键是尽早地用药物或手术方法缓解瞳孔阻滞,房角损伤≥２个象限者加用滤过性手术。     

碱性成纤维细胞生长因子在人晶体上皮细胞的蛋白检测研究

研究成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ,ｂＦＧＦ）对人晶体上皮细胞（ｈｕｍａｎｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｓ,ＨＬＥＣｓ）的促增殖作用。方法：采用免疫组织化学检查（ＡＢＣ法）来检测人晶体上皮细胞上ｂＦＧＦ蛋白水平,并用图像分析进行相对定量。结果：定性及定量地证明了人晶体上皮细胞有ｂＦＧＦ蛋白存在。结论：阐明了人晶体上皮细胞本身存在的ｂＦＧＦ以蛋白的形式参与了术后后囊混浊的形成。     

高效液相色谱—间接光度检测法同时测定血清和尿中5种氨基苷类抗生素

目的：建立一种直接分离测定体液中庆大霉素、丁胺卡那霉素、妥布霉素、西梭霉素和乙基西梭霉素等５种氨基苷类抗生素的高效液相色谱－间接光度检测（ＨＰＬＣ－ＩＰＤ）法。方法：在流动相中加入具有紫外检测响应的检测剂烟酰胺,用紫外检测器直接测定紫外吸收很差的上述５种药物。Ｃ１８固定相,流动相为含烟酰胺０５ｍｍｏｌ·Ｌ－１、庚烷磺酸钠５ｍｍｏｌ·Ｌ－１和磷酸００５ｍｏｌ·Ｌ－１的甲醇－乙腈－水（２７∶１８∶５５）混合溶液。结果：血清和尿样平均回收率均大于９６％,日内和日间ＲＳＤ均小于６％。并测定了肌注此类药物病人的血清和尿样品。结论：该法适于体液中氨基苷类药物检测。