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排序方式: 共有269条查询结果,搜索用时 15 毫秒
101.
102.
Expression of vascular endothelial growth factor in patients with testicular germ cell tumors as an indicator of metastatic disease 总被引:4,自引:0,他引:4
Fukuda S Shirahama T Imazono Y Tsushima T Ohmori H Kayajima T Take S Nishiyama K Yonezawa S Akiba S Akiyama S Ohi Y 《Cancer》1999,85(6):1323-1330
BACKGROUND: Angiogenesis is essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) and thymidine phosphorylase (TP)/platelet-derived endothelial cell growth factor (PD-ECGF) are involved in increased angiogenic activity and disease progression in solid tumors. However, there is no information regarding the association of these angiogenic factors with clinicopathologic findings in testicular germ cell tumors (GCTs). METHODS: The authors examined the expression of VEGF and TP as well as microvessel density in GCTs and their association with clinicopathologic findings. Expression of VEGF and TP and microvessel density were examined immunohistochemically in 80 GCTs, including 33 seminomas (25 tumors with organ-confined disease and 8 with metastasis) and 47 nonseminomatous testicular GCTs (NSGCTs) (20 tumors with organ-confined disease and 27 with metastasis). Expression of VEGF also was examined in four GCTs and one nonneoplastic testis by immunoblotting. RESULTS: VEGF protein was expressed more highly in GCTs compared with nonneoplastic testes. VEGF expression in GCTs was correlated significantly with microvessel count (P < 0.001). Both VEGF expression and microvessel count were correlated with metastasis in seminoma (P = 0.008 and P < 0.001, respectively), but only VEGF expression was identified as statistically significant by multiple regression analysis (P = 0.006). Conversely, four variables (VEGF expression, microvessel count, the presence of venous invasion, and the presence of embryonal carcinoma elements in the primary tumor) were correlated with metastasis in NSGCT (P < 0.001, P < 0.001, P = 0.004, and P = 0.029, respectively). However, multiple regression analysis revealed that only VEGF expression and microvessel count were significant factors for metastasis (P < 0.007 and P < 0.001, respectively). In contrast, high levels of TP were observed in infiltrating cells, but not in the majority of cancer cells. CONCLUSIONS: The findings of the current study suggest that VEGF expression is involved in tumor development, angiogenesis, and metastasis in GCT. 相似文献
103.
Tadashi Iwanaka Atsuyuki Yamataka Sadashige Uemura Hiroomi Okuyama Osamu Segawa Masaki Nio Joji Yoshizawa Makoto Yagi Satoshi Ieiri Hiroo Uchida Hiroyuki Koga Masahito Sato Hideki Soh Hiroshi Take Ryuichiro Hirose Hiroaki Fukuzawa Masaru Mizuno Toshihiko Watanabe 《Asian journal of endoscopic surgery》2015,8(4):390-407
104.
Yasuo Yamamoto Jir Arata Midori Tokuyama Makoto Take Nozomi Nohara 《The Journal of dermatology》1983,10(1):63-66
Hyaluronic acid synthetase (HAS) activity of lyophilized skin from a 49-year-old Japanese man with lichen myxedematosus was assayed. The HAS activity was very high in both diseased and normal appearing skin. 相似文献
105.
K Fukushima T Ohara A Take H Horimi T Hasegawa M Kanazawa I Yoshida 《[Zasshi] [Journal]. Nihon Kyōbu Geka Gakkai》1989,37(2):195-202
Sixteen patients with the malignant mediastinal tumors compromising the superior vena cava (SVC) and brachiocephalic veins (BCV) were treated from April 1974 to March 1987. SVC reconstruction by prosthesis combined with or without tumor resection were performed in 6 cases and removal of the tumor with partial resection of SVC and right BCV in one case. Two patients died within one month and two patients survived over 5 years postoperatively. Tumor resection, removal of the right lung and SVC wall, and patch angioplasty were performed in one patient who died three and one half months postoperatively. Resection of the tumor and left BCV were performed in 4 patients. Segmentally resected left BCV was reconstructed with interposed graft in two, prosthesis was implanted between left BCV and right atrium in one and no venous reconstruction was attempted in one. Three patients are alive and one patient died six and one half years after operation. Exploratory mediastinotomy was carried out in 3 cases and radiation therapy alone in one case. All but one patients died within one year after diagnosis. Though the long term results of the surgical treatment for malignant mediastinal tumor was not satisfactory, extensive resection of the tumor was worth attempting, as the patency rate of the prosthetic venous reconstruction was improved. 相似文献
106.
The preoptic/anterior hypothalamus (POA) is one of the major brain regions where cytokines and their related mediators (i.e., prostaglandins) exert diverse actions. In the present study, the modulatory effects of prostaglandin E(2) (PGE(2)) on the glutamate-induced membrane currents were examined using perforated-patch clamp method in rat POA neurons that had been mechanically dissociated by vibration without enzyme treatment. Application of glutamate through U-tube induced a slow outward current following fast inward ionotroic current at a holding membrane potential of -30 mV. The slow outward current was also induced by N-methyl-d-aspartate (NMDA), accompanied by an increased membrane conductance, and inhibited by perfusion with Ca(2+)-free solution, tetraethylammonium chloride (TEA), and apamin, suggesting a Ca(2+)-dependent K(+) current (KCa) activated by Ca(2+) entry through NMDA channels. Perfusion with PGE(2) at 0.1-10 microM, a principal mediator of fever and neuroendocrine control at the POA, did not produce apparent current by itself, but selectively potentiated the glutamate- or NMDA-induced KCa without affecting inward currents. The KCa induced by activation of NMDA receptors may serve as a feedback mechanism and the modulatory effects of PGE(2) on the KCa may have an important physiological significance. 相似文献
107.
Anorectal malformation associated with a perineal protrusion of the rectal mucosa: case presentation
Masato Shinkai Kyoko Mochizuki Yoshihiro Hirata Shohei Honda Norihiko Kitagawa Hiroshi Take Youkatsu Ohhama Mio Tanaka Yukichi Tanaka 《Pediatric surgery international》2009,25(9):815-817
We present a rare case of a female neonate with an imperforate anus associated with a perineal mass which may correspond to
an extrophied rectal duplication. Associated anomalies were thoracic hemivertebrae and a multicystic dysplastic kidney. Excision
of the perineal lesion followed by anal transplantation and perineal reconstruction corrected the anomaly. 相似文献
108.
Aplasia of the dorsal pancreas and choledochal cyst 总被引:2,自引:0,他引:2
Oyachi N Ohhama Y Take H Fukuzato Y Murakami T Kitagawa N Kudo H 《Pediatric surgery international》2006,22(6):557-559
The authors describe a rare case of choledochal cyst and aplasia of the dorsal pancreas complicated with chronic pancreatitis. A 9-year-old boy presented with obstructive jaundice. After biliary drainage using pericutaneous transhepatic gallbladder drainage (PTGBD) technique, the patient underwent choledochal cyst excision with Roux-en-Y hepaticojejunostomy. The association of choledochal cyst and aplasia of the dorsal pancreas with chronic pancreatitis has never been reported previously. 相似文献
109.
Mariella T. Simon Shaya S. Eftekharian Sacha Ferdinandusse Sha Tang Take Naseri Muagututi'a Sefuiva Reupena Stephen T. McGarvey Ryan L. Minster Daniel E. Weeks The Samoan Obesity Lifestyle Genetic Adaptations Study Group Daniel D. Nguyen Sansan Lee Katarzyna A. Ellsworth Frédéric M. Vaz David Dimmock James Pitt Jose E. Abdenur 《American journal of medical genetics. Part A》2021,185(1):157-167
Mutations in the short‐chain enoyl‐CoA hydratase (SCEH) gene, ECHS1, cause a rare autosomal recessive disorder of valine catabolism. Patients usually present with developmental delay, regression, dystonia, feeding difficulties, and abnormal MRI with bilateral basal ganglia involvement. We present clinical, biochemical, molecular, and functional data for four affected patients from two unrelated families of Samoan descent with identical novel compound heterozygous mutations. Family 1 has three affected boys while Family 2 has an affected daughter, all with clinical and MRI findings of Leigh syndrome and intermittent episodes of acidosis and ketosis. WES identified a single heterozygous variant in ECHS1 at position c.832G > A (p.Ala278Thr). However, western blot revealed significantly reduced ECHS1 protein for all affected family members. Decreased SCEH activity in fibroblasts and a mild increase in marker metabolites in urine further supported ECHS1 as the underlying gene defect. Additional investigations at the DNA (aCGH, WGS) and RNA (qPCR, RT‐PCR, RNA‐Seq, RNA‐Array) level identified a silent, common variant at position c.489G > A (p.Pro163=) as the second mutation. This substitution, present at high frequency in the Samoan population, is associated with decreased levels of normally spliced mRNA. To our understanding, this is the first report of a novel, hypomorphic allele c.489G > A (p.Pro163=), associated with SCEH deficiency. 相似文献
110.