后肢固定大鼠腓肠肌酶活性与银杏叶提取物EGB761的影响

目的:观察银杏叶提取物EGB761对后肢固定大鼠腓肠肌Na -K -ATP酶及Ca2 -ATP酶活性的影响,探讨其对失用性骨骼肌萎缩的防治作用。方法:实验于2005-04/2006-03在广州南方医院创伤骨科实验室(广东省重点实验室)完成。①实验分组:雄性SD大鼠24只,体质量200～260g,随机数字表法分为空白组、对照组、实验组,每组8只。②实验方法:制备大鼠骨骼肌失用性萎缩模型,将大鼠右后肢用可塑性石膏由足跖管型固定至膝上1cm处,膝关节固定于屈曲位100°,踝关节固定于跖屈60°,对照组给予蒸馏水3mL/d灌胃,实验组给予银杏叶提取物EGB761水溶液灌胃(灌胃剂量120mg/(kg·d),浓度14g/L)。③实验评估:4周后切取右后肢腓肠肌,去除腱膜、肌腱和神经,进行酶活性检测。结果:纳入大鼠24只,均进入结果分析。与空白组相比,对照组和实验组腓肠肌Na -K -ATP酶及Ca2 -ATP酶活性显著下降(P<0.05)。与对照组相比,实验组Na -K -ATP酶及Ca2 -ATP酶活性显著高于对照组(P<0.05)。结论:银杏叶提取物EGb761能够显著提高失用性萎缩骨骼肌Na -K -ATP酶及Ca2 -ATP酶活性,从而有效抑制骨骼肌失用性萎缩的发生。     

HPLC法测定藏药二十五味珊瑚丸中乌头碱限量

目的:建立HPLC法测定藏药二十五味珊瑚丸中乌头碱的限量.方法:采用Phenomenx Gemini DOS(4.6 mm×250mm,5μm)色谱柱,以0.04mol·L-1三乙胺(用磷酸调节至pH 3.0±0.1)-甲醇(60:40)为流动相,流速1.0 mL·min-1,检测波长235 nm,柱温40℃.结果:乌头碱在1～120μg范围内,存在良好的线性关系(r＝0.999 8);检测限为10 ng.结论:本方法快速简便,准确可靠,可用于二十五味珊瑚丸的质量控制.     

CD44(+)/CD24(-) cells are transit progenitors and do not determine the molecular subtypes and clinical parameters in breast carcinomas

CD44(+)/CD24(-) cells have been associated with breast cancer stem/progenitor cell features. However, the status of this phenotype cells in normal, benign and malignant breast tissues has not been studied, and the clinical correlation of this subpopulation in breast cancer is not fully understood. The present study sought to identify these cells in a series of normal, benign, and malignant breast tissues and explore their correlation to the molecular subtypes of breast carcinoma and conventional pathological features. Double-staining immunohistochemistry (DIHC) of CD44 and CD24 was performed on 30 normal breast tissues, 30 breast fibroadenomas (FA), 60 breast invasive ductal carcinomas (IDC), and 3 breast cancer cell lines (MCF-7, MDA-MB-468, and MDA-MB-231). In the normal breast tissues and FAs, three phenotypes were observed including CD44(+)/CD24(+), CD44(+)/CD24(-), and CD44(-)/CD24(-) cells. In the IDCs, CD44(-)/CD24(+) cells were detected, in addition to the three aforementioned phenotypes. The strong positive rate (+++, incidence >60%) of CD44(+)/CD24(-) was significantly increased from normal breast tissue, FAs to IDCs (0.0%-->6.7%-->21.7%). However, the CD44(+)/CD24(-) cells didn't correlate with ages of patients, lymph node metastasis, tumor size, molecular subtypes, and the expression of ER, PR, HER-2, PS2, Bcl-2, nm23. The proportion of CD44(+)/CD24(-) cells in MCF-7, MDA-MB-468, and MDA-MB-231 was about 1, 5, and 80%, respectively. The results indicate that the CD44(+)/CD24(-) cells are transit progenitors and have no association with the molecular subtypes and clinicopathological parameters in the IDCs.     

Bacterial expression of antigenic sites A and D in the spike protein of transmissible gastroenteritis virus and evaluation of their inhibitory effects on viral infection

The spike (S) protein is a key structural protein of coronaviruses including, the porcine transmissible gastroenteritis virus (TGEV). The S protein is a type I membrane glycoprotein located in the viral envelope and is responsible for mediating the binding of viral particles to specific cell receptors and therefore specific cell types. It is also an important immune target for the host in neutralizing the virus. Four antigenic sites A, B, C, and D that reside near the N-terminal domain have been defined in the S protein. Of these, the region encoding antigenic sites A and to a lesser extent D, herein defined as S-AD, are most critical in eliciting host neutralizing antibodies. Herein, we enzymatically amplified, cloned, and expressed the S-AD fragment from TGEV in the prokaryotic expression vector, pET-30a. Maximum protein expression was achieved at 30°C over a 5-h period post-induction. Rabbit polyclonal antiserum was generated using recombinant S-AD (rS-AD) protein. In contrast to prior studies showing no activity with bacterially produced S protein, results indicated that polyclonal serum recognized TGEV-infected cells and reduced infection by 100%. Furthermore, the truncated rS-AD peptide was able to bind to the surface of cells from swine testes in a competitive manner and completely inhibit viral infection.     

艾滋病健康知识教育对收容教育女性艾滋病乐观偏差的效果评价及启示

目的考察艾滋病健康知识教育前后被收容教育女性对艾滋病乐观偏差的特点及其对艾滋病乐观偏差的差异。方法用艾滋病事件量表、艾滋病知识量表对温州市收容教育所59名女性收容教育者进行研究。结果收容教育女性同时存在艾滋病Ⅰ型乐观偏差和Ⅱ型乐观偏差;收容教育女性在健康知识教育后对艾滋病知识的掌握水平显著提高,健康知识教育可以显著降低他们对治愈艾滋病的乐观偏差。结论艾滋病健康知识教育可从认知上改变收容教育女性对艾滋病的态度,有效降低他们对治愈艾滋病的乐观偏差,减少其对“即使感染了艾滋病,自己也会比另q人容易治愈”的不合理想法。     

284名饭店打工妹抑郁现状及其影响因素研究

目的了解饭店打工妹抑郁现状,探讨其与社会资本和社会网络的关系。方法本研究为横断面调查。选取合肥市12家饭店、蚌埠市14家饭店为研究现场,以在饭店打工、农村户口、16～24岁、未婚女性为研究对象。采用流调中心用抑郁量表（CES-D）对其抑郁症状进行评定。抑郁评分≥20分界定为有抑郁症状;采用自编问卷评估社会资本和社会网络。结果调查对象平均年龄20.18岁,抑郁症状检出率为46.5%。多因素Logistic回归分析结果显示,打工妹年龄平均每增加3岁,抑郁症状发生的危险性降低到0.609倍;单亲或父母再婚家庭是抑郁症状发生的危险因素（OR=2.542）;认知社会资本为中下等、中上等及上等者发生抑郁症状的危险性较下等者均降低（OR=0.483、0.511、0.254）。结论饭店打工妹抑郁症状检出率高,尤其是年龄小、来自单亲或父母再婚家庭者。完善社会保障制度,增强社会诚信氛围、加大社会支持力度,可提高认知社会资本,降低抑郁症状发生的危险性。     

缬沙坦对肝硬化患者血HA、CG及PAI 1的影响

目的 探讨缬沙坦对肝硬化患者血HA、CG及PAI-1的影响.方法 将36例肝硬化患者随机分为治疗组和对照组各18例.对照组给予常规治疗,治疗组在此基础上口服缬沙坦80 mg/d,疗程1个月.治疗前后采血,用放射免疫法检测血清HA、CG的水平,酶联免疫法测定血浆PAI-1的水平.结果 治疗组患者HA、CG及PAI-1的水平较治疗前下降.对照组上述指标的下降无统计学意义.结论 缬沙坦能够降低肝硬化患者血HA、CG及PAI-1的水平,从而具有一定抗肝纤维化的作用.     

硝普钠与硝酸甘油治疗急性左心衰的疗效观察

目的：观察硝普钠与硝酸甘油治疗急性左心衰的疗效和安全性。方法：294例急性左心衰患者随机分成硝普钠治疗组158例、硝酸甘油治疗组136例,对照观察静脉给药后5天两组NYHA分级心功能改善程度。结果：两组治疗右心功能改善程度差异显著。结论：微泵注射硝普钠治疗急性左心衰起效快、疗效好、不良反应少、安全可靠。     

Feasibility Studies of Nebulized SARS-CoV-2 Neutralizing Antibody in Mice and Cynomolgus Monkeys

Pharmaceutical Research - Neutralizing antibodies, administrated through intravenous infusion, have shown to be highly efficacious in treating mild and moderate COVID-19 caused by SARS-CoV-2...