Ischemia and reperfusion reduce the endogenous basic fibroblast growth factor in rat skeletal muscles: an immunohistochemical study.

Polyclonal antibodies directed against human recombinant basic fibroblast growth factor were used in immunohistochemical studies to localize this growth factor in normal and wounded rat skeletal muscles. According to the intensity of the stain, three main classes of fibers could be identified: the strongly, moderately, and weakly stained fibers. Basic fibroblast growth factor immunoreactivity was found mainly in the extracellular matrix, primarily in the endomysium, which includes the heparin-containing basal lamina, and also in the capillary basal membrane of both normal and wounded muscles; however, the signal intensity was much stronger in normal muscles. The distribution of basic fibroblast growth factor in wounded muscles became markedly heterogeneous and sparse. After 4 hours of ischemia, about 40% of skeletal muscle fibers lost their basic fibroblast growth factor immuno-reactivity. Muscles which underwent 4 hours of ischemia and 24 hours of reperfusion had only a diminished basic fibroblast growth factor immunoreactivity. The pathologic results supported the concept of destroyed cell connection and fiber necrosis in ischemic and reperfused muscles. Potential mechanisms involved in this reduced concentration of basic fibroblast growth factor in wounded muscles may include oxygen free radical activation, a generalized effect of the inflammatory response, and reduced secretion of endogenous basic fibroblast growth factor. These results are only partially compatible with the established mitogenic role of this growth factor and suggest that a reduction of endogenous fibroblast growth factor may partly contribute to a delay in wound healing.     

Protective effect of Re-LPS antiserum on experimental multiple system organ failure.

This study examines the possible beneficial effect of Re-LPS (F515) antiserum on experimental multiple system organ failure (MSOF) in rabbits. The results showed that the plasma LPS level was significantly decreased, and it took a shorter period to clear up LPS in experimental than in control rabbits after receiving Re-LPS antiserum. Pretreatment with antiserum can markedly improve the function of the liver, lungs, kidneys, blood and gastrointestinal tract. The MSOF incidence in the group of rabbits receiving immune sera was only 11.2% and the survival rate was raised by about 40.0%. The results suggest that early passive immunotherapy may neutralize gut-derived endotoxin, inhibit endotoxin-induced mediators release and prevent development of severe complications due to sepsis. It is therefore postulated that LPS core antiserum may provide a prophylactic effect on the development of experimental MSOF.

     

尖锐湿疣中乳头瘤病毒感染与P53和ki-67表达的关系

目的　探讨尖锐湿疣中乳头瘤病毒 (HPV)感染与 P5 3和 ki- 6 7的关系。　方法　采用免疫组织化学方法对 82例尖锐湿疣的 HPV感染、P5 3和 ki- 6 7的表达进行观察。　结果　 HPV阳性者 2 4例 (2 9.3% ) ,P5 3阳性表达 2 0例 (2 4 .4 % ) ,ki- 6 7阳性表达 2 1例 (2 5 .6 % )。2 4例 HPV阳性者中 ,P5 3阳性表达 10例 (41.7% ) ,ki- 6 7阳性表达 11例 (45 .8% ) ,经关联分析 ,皮损中 HPV感染与 P5 3(χ2 =4 .2 4 7,P<0 .0 5 )和 ki- 6 7(χ2 =5 .6 8,P<0 .0 5 )阳性表达均有显著关联 ;而 P5 3与 ki- 6 7两者均阳性者仅有 6例 (χ2 =0 .98,P>0 .0 5 ) ,无明显关联。　结论　在尖锐湿疣中 HPV感染伴随 P5 3和 ki- 6 7表达的增加     

人大肠癌细胞株（HT29）与人多器官血管内皮细胞粘附数目及形态学观察

为了模拟体内癌细胞与血管内皮粘附的过程，用人大肠癌细胞与人多器官血管内皮细胞共同培养，观察到与不同血管内皮粘附的癌细胞数有显著性统计学差异（Ｐ＜０．０５—０．０１）。与门静脉、肠系膜静脉内皮粘附的癌细胞数多，细胞表面微绒毛多，丝状突起细长，伪足多且长；与大隐静脉内皮粘附的癌细胞数少，细胞表面突起也细小。这表明不同的血管内皮对癌细胞的粘附是具有选择性的。     

植酸磷的测定──离子交换法

介绍用离子交换法测定植酸磷的含量。对消化终点、最适的提取时间、离子交换树脂的分离效果进行了试验。黄豆粉、窝窝头、豆腐干植酸测得值的变异系数分别为２．１６％、４．９２％、１．７８％，回收率依次为１０３．３８％、１０２．８１％、１０３．４１％。植酸标准品的植酸含量理论值为５７．６４％，本法测定值为５５．２３％，相对误差为２．１４％。本法的精密度及准确度均符合要求。     

后勤社会化医院增活力--一家地方医院的后勤社会化改革路径

改革医院后勤管理模式,是现代医院发展的需求。通过深化后勤社会化服务,将有效促进医院整体水平的提升。     

The prevalence of LRRK2 Gly2385Arg variant in Chinese Han population with Parkinson's disease.

We conducted a case-control study to determine the prevalence of the LRRK2 Gly2385Arg variant in patients with Parkinson's disease in Han population in mainland China. Heterozygous LRRK2 Gly2385Arg variant was identified in 14 of 235 patients with Parkinson's disease (5.69%), but not in 214 unrelated healthy controls. Multivariate analysis indicated the frequency of Gly2385Arg variant in the female patients with early age at onset is higher than their male counterparts. The founder haplotype analysis showed the variant carriers shared the same founder. Clinically, the LRRK2 Gly2385Arg carriers presented with classical Parkinson's disease symptoms. Our study indicates that the LRRK2 Gly2385Arg variant is a potential ethnic-specific genetic risk factor of Parkinson's disease within Chinese Han ethnicity.     

人群高血压干预效果评价

本文按照世界卫生组织高血压诊断标准,采用流行病学前瞻性观察方法,随机选择两个相似人群,对年龄≥35岁居民5441人,进行连续三年高血压患者系统管理和对照组自然趋势对比分析.研究结果表明:高血压患病率,三年后对照组上升近5%(P<0.05),干预组基本保持稳定(P>0.05).血压均值变化,干预组收缩压、舒张压逐年降低,规律性降压药物治疗由31.4%提高到53.1%,高血压控制率达90.5%,干预人群总体血压水准低于对照人群.脑卒中发病率较对照组有降低趋势.本研究达到了世界卫生组织提出的高血压早期预防的管理目标.     

后颅凹成血管细胞瘤的影像学特点及其诊断

目的：探讨成血管细胞瘤的影像学特点。材料与方法：报道１４例成血管细胞瘤的ＣＴ和ＭＲＩ所见，分析肿瘤的特点。结果：囊性肿瘤，在ＭＲＩ上表现为囊性病灶和附壁肿瘤结节；在ＣＴ上表现为该典型征象占８１．８％。混合性肿瘤３例，在ＣＴ和ＭＲＩ上表现实质性肿块、偏心性囊变和均一显著强化；后者还可见肿瘤旁和肿瘤内血管流空。结论：ＣＴ和ＭＲＩ均是诊断成血管细胞瘤的有效检查方法。成血管细胞瘤的特征性影像学表现是囊肿和突入囊内的壁结节，以及肿瘤的异常显著增强     

医源性腓总神经伤分析

目的：总结40a来收治的48例医源性腓总神经损伤的临床资料，探讨其损伤机制和特点。方法：通过随访，依据其损伤原因和机制分类统计。结果：牵拉压迫伤33例，占69％；手术切割伤12例，占25％。导致腓总神经损伤的原因中以石膏压迫为最多，占37％，牵伸性损伤占31％。结论：骨科医师必须具备丰富的解剖知识和责任心，重在预防，一旦发生损伤，应早期诊断，采用有效的方法及时治疗。