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941.
VVA2 (volvatoxin A chain 2) is a cardiotoxic protein purified from Volvariella volvacea. Its biological activities include hemolysis, writhing reaction, neurotoxicity, and ventricular systolic arresting activity. The cytotoxicity of VVA2 was mainly considered due to its pore-forming activity. Here we report a novel biological activity of its variants VVA2 I82E/K86K as a duplex-specific nuclease. Recombinant VVA2 variant I82E/L86K (Re-VVA2 I82E/L86K), deprived of the oligomerization property, shows increased nuclease activity compared to VVA2. Re-VVA2 I82E/L86K converts supercoiled DNA (Replicative form I, RF I) into nicked form (RF II) and linear form (RF III) in the presence of Mg2+ or Mn2+. Besides plasmid DNA, it also exhibits nuclease activity on E. coli genomic DNA rather than ssDNA or RNA. Re-VVA2 I82E/L86K preferentially cleaves dG-dC-rich dsDNA regions and shows the best performance at pH 6–9 and 55 °C. Our structure–function study has revealed amino acid E111 may take an active part in nuclease activity through interacting with metal ions. Based on the sequences of its cleavage sites, a “double-hit” mechanism was thereby proposed. Given that Re-VVA2 I82E/L86K did not exhibit the conserved nuclease structure and sequence, it is considered an atypical duplex-specific nuclease. 相似文献
942.
Mariola Usovsky Pengyin Chen Dexiao Li Aiming Wang Ainong Shi Cuiming Zheng Ehsan Shakiba Dongho Lee Caio Canella Vieira Yi Chen Lee Chengjun Wu Innan Cervantez Dekun Dong 《Viruses》2022,14(6)
This review summarizes the history and current state of the known genetic basis for soybean resistance to Soybean mosaic virus (SMV), and examines how the integration of molecular markers has been utilized in breeding for crop improvement. SVM causes yield loss and seed quality reduction in soybean based on the SMV strain and the host genotype. Understanding the molecular underpinnings of SMV–soybean interactions and the genes conferring resistance to SMV has been a focus of intense research interest for decades. Soybean reactions are classified into three main responses: resistant, necrotic, or susceptible. Significant progress has been achieved that has greatly increased the understanding of soybean germplasm diversity, differential reactions to SMV strains, genotype–strain interactions, genes/alleles conferring specific reactions, and interactions among resistance genes and alleles. Many studies that aimed to uncover the physical position of resistance genes have been published in recent decades, collectively proposing different candidate genes. The studies on SMV resistance loci revealed that the resistance genes are mainly distributed on three chromosomes. Resistance has been pyramided in various combinations for durable resistance to SMV strains. The causative genes are still elusive despite early successes in identifying resistance alleles in soybean; however, a gene at the Rsv4 locus has been well validated. 相似文献
943.
944.
The fast heating and quenching of laser cladding increase the internal stresses in the cladding layer. Moreover, the quick condensation of the molten pool leads to an uneven distribution of the internal elements and coarse grains of the structure. To address the above defects and increase the molding quality of laser cladding, an electromagnetic field was introduced into the laser cladding technique, and the effects of the external assisted electromagnetic field on the mixed metal fluid in the molten pool were explored. On this basis, the action of the electromagnetic field on the flow states of the molten pool was further analyzed. The results demonstrate that after introducing electromagnetic assistance, the material flow in the molten pool accelerated as a response to the periodic changes in electromagnetic forces and the influences of the electromagnetic field on crystallization, thus refining the grains and improving the grain distribution uniformity in the cladding layer. The dendritic crystals in the cladding layer decreased, while the isometric crystals and the cellular-like dendrites increased. The element distribution in the cladding layer increased in uniformity. Additionally, this method can decrease the dilution rate of the cladding layer and improve its overall hardness. A laser-cladding test of the Ni-based powder was carried out on the AISI 1045 steel surface under the coaxial powder-feeding mode. Moreover, the influences of the electromagnetic field on the microstructure of the laser-cladding layer were compared, and the causes of the changes were disclosed. 相似文献
945.
Zhengyuan Wang Xin Xu Shanxi Gao Chunxiang Wu Qi Song Zehuan Shi Jin Su Jiajie Zang 《Nutrients》2022,14(12)
Effective nutrition and exercise interventions may improve sarcopenia in the elderly. The purpose of our study was to investigate the effectiveness of Internet-based nutrition and exercise interventions in the elderly with sarcopenia. Participants were divided into 4 groups: control, nutrition, exercise, and comprehensive (nutrition plus exercise) groups; there was at least 50 participants in each group. Our trial lasted 12 weeks. We conducted dietary and exercise interventions through an app and collected feedback from the participants every three weeks. Information on the diet, skeletal muscle mass, and muscle function was collected before and after the interventions. The comprehensive group had higher high-quality protein intake than the control (p = 0.017) and exercise (p = 0.012) groups. After the interventions, we obtained differences in skeletal muscle mass, skeletal muscle mass/height2, skeletal muscle mass/weight, muscle mass/BMI, and skeletal muscle mass/body fat percentage (p < 0.05). Changes in average daily energy and total daily protein intakes were not significantly different; however, there was an overall improvement in the intervention groups relative to baseline data. There were no changes in the average daily time of moderate physical activity. The Internet was an effective tool of nutrition intervention in the elderly with sarcopenia. The Internet-based nutrition intervention improved high-quality protein intake and skeletal muscle mass in the elderly with sarcopenia. 相似文献
946.
王雅杰 王璨 方芳 史从宁 蒋迪 张国军 吕虹 杨华卫 常铮 韩金利 赵传赞 耿慧娟 唐明忠 王美容 张艳 刘亚楠 康熙雄 高华方 Wang Yajie Wang Can Fang Fang Shi Congning Jiang Di Zhang Guojun Lü Hong Yang Huawei Chang Zheng Han Jinli Zhao Chuanzan Geng Huijuan Tang Mingzhong Wang Meirong Zhang Yan Liu Yanan Kang Xixiong Gao Huafang 《首都医科大学学报》2007,28(2):140-144
目的利用基因芯片技术进行常见革兰阳性细菌的细菌鉴定和耐药性检测。方法用生理生化鉴定和药敏纸片法确定445份革兰阳性细菌菌株的细菌种类和耐药性,盲法进行革兰阳性细菌鉴定与耐药检测基因芯片的检测,用SPSS 10.0 for Windows软件统计分析。结果与临床常规方法相比,耐药检测基因芯片鉴定指标的灵敏度均>96%,特异度均>98%;耐药检测指标的灵敏度均>94%,特异度均>90%。χ2检验结果P值均>0.05,基因芯片检测结果与临床常用方法检测结果差异无统计学意义。Kappa值均>0.75,2种方法一致性较好。结论基因芯片技术对细菌鉴定和耐药检测有一定的临床应用价值。 相似文献
947.
手术切除巨大脑动静脉畸形(arteriovenous malformations,AVMs)术中或术后可能发生一种严重的并发症,表现为脑内出血、脑肿胀。1978年Spetzler医师将其命名为正常灌注压突破(normal perfusion pressure breakthrough,NPPB)。文献报道NPPB发生率为1%~10%,手术治疗巨大AVMs的术后并发症令人不满,致残率和病死率高达50%。本研究采用手术中栓塞加切除联合治疗巨大脑AVMs,研究手术前、后局部脑皮质血流的变化,栓塞后逐步阻断动脉与静脉的短路,有助于将AVMs与正常脑组织分离,预防手术中和手术后再出血以及NPPB的发生。因此,手术中栓塞加切除联合治疗巨大脑AVMs是降低手术后并发症的有效措施。 相似文献
948.
949.
950.
高龄患者白内障超声乳化吸除联合人工晶状体植入术 总被引:2,自引:0,他引:2
目的:探讨高龄患者白内障超声乳化吸除联合人工晶状体植入手术的可行性和安全性。方法:对105例(142眼)高龄患者进行白内障超声乳化吸除联合人工晶状体植入术,以88例(142眼)低龄老年性白内障患者作对照组。结果:两组术后视力提高差异无显著性(P>0.05),手术并发症有明显差异(P<0.01),两组均无因全身疾病而中断手术者。结论:对高龄患者施行白内障超声乳化吸除联合人工晶状体植入术是相对安全的,只要有复明的希望,就应尽早手术治疗。 相似文献