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11.
Motoi Baba Masato Takahashi Katsushige Yamashiro Hideki Yokoo Moto Fukai Masanori Sato Mitsuchika Hosoda Toshiya Kamiyama Akinobu Taketomi Hiroko Yamashita 《Surgery today》2016,46(7):843-851
Purpose
Recent studies have indicated that constitutive NF-κB activity could be involved in the proliferation of triple-negative breast cancer.Methods
The NF-κB/p65 expression and the effects of a NF-κB inhibitor, (?)-DHMEQ, were examined in triple-negative MDA-MB-231 breast cancer cells. Women with triple-negative breast cancer treated with neoadjuvant chemotherapy between 2002 and 2012 were retrospectively analyzed for their expression of NF-κB/p65, Bcl2 and Ki67 by immunohistochemistry in pre- and post-treatment specimens. The factors predicting the response to neoadjuvant chemotherapy and the prognosis were analyzed.Results
NF-κB/p65 was predominantly expressed in the cytoplasm of MDA-MB-231 cells. Of 34 triple-negative breast cancer patients, positive staining for NF-κB/p65 expression was detected in the nuclei of a few cells in seven tumors before neoadjuvant chemotherapy, while the expression of NF-κB/p65 in the cytoplasm was detected in almost all tumor cells of 33 tumors. The expression levels of NF-κB/p65 were not associated with the response to neoadjuvant chemotherapy, although the cytoplasmic NF-κB/p65 staining intensity was significantly decreased in the post-treatment tumor samples compared with the pretreatment samples. All patients whose tumors showed strong cytoplasmic NF-κB/p65 expression before neoadjuvant chemotherapy are currently disease free.Conclusion
Our results suggest that strong cytoplasmic NF-κB/p65 expression could be a prognostic marker for patients with triple-negative breast cancer.12.
Eyitayo O. Owolabi RN PhD Samukelisiwe Nyamathe MBChB Conran Joseph PhD Lee-Ann Jacobs-Nzuzi Khuabi PhD Rene G. English MBChB MMed FCPHM PhD Adriaan Vlok MBChB FC MMed PhD Elaine Erasmus FCEM MMed EM DipPEC MBChB Heike I. Geduld MBChB FCEM MMed Hendrick J. Lategan BSc MBBCh MMed FCEM Kathryn M. Chu MD MPH FACS FASCRS 《Journal of evaluation in clinical practice》2023,29(2):380-391
13.
Sachiyo Yoshio Tomonari Shimagaki Ryuki Hashida Takumi Kawaguchi Yuriko Tsutsui Yuzuru Sakamoto Yuichi Yoshida Hironari Kawai Shiori Yoshikawa Taiji Yamazoe Taizo Mori Yosuke Osawa Shinji Itoh Moto Fukai Tomoharu Yoshizumi Akinobu Taketomi Masaki Mori Tatsuya Kanto 《Hepatology research》2021,51(7):803-812
14.
van Gils FC; van Teeffelen ME; Neelis KJ; Hendrikx J; Burger H; van Leen RW; Knol E; Wagemaker G; Wognum AW 《Blood》1995,86(2):592-597
To understand the hematopoietic and nonhematopoietic responses to interleukin-3 (IL-3), expression of cell-surface IL-3 receptors (IL-3R) was examined on bone marrow (BM) cells and peripheral blood (PB) cells of rhesus monkeys during the course of in vivo IL-3 treatment. Whereas IL-3R expression is low in untreated monkeys, IL-3 administration led to a gradual increase in both low- and high-affinity binding sites for IL-3. This increase reflected the total number of cells expressing IL- 3Rs, as detected by flow cytometry using biotinylated IL-3. Most of these IL-3R+ cells in both BM and PB could be characterized as basophilic granulocytes that contained high levels of histamine. In contrast to the effect on these differentiated cells, IL-3 administration did not significantly alter the low level IL-3R expression on immature, CD34+ cells. Further flow cytometric analysis using biotinylated growth factors showed that the IL-3R+ basophils also expressed receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), but not for IL-6 or Kit ligand. These findings indicated that the IL-3R+ cells included neither monocytes, which express GM-CSFRs and IL-6Rs abundantly, nor mast cells, which express c- kit. By combining flow cytometric and Scatchard data, it was calculated that the basophils contain as many as 1 to 2 x 10(3) high-affinity IL- 3Rs and 15 to 30 x 10(3) low-affinity sites. The finding that in vivo IL-3 treatment leads to the production of large numbers of cells that express high levels of IL-3R and are capable of producing histamine provides an explanation for the often severe allergic reactions that occur during prolonged IL-3 administration. It also indicates that IL- 3, in addition to its direct effects on hematopoietic cells, may also stimulate hematopoiesis through the release of secondary mediators such as histamine by IL-3-responsive mature cells. 相似文献
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Early pregnancy complications: endovaginal sonographic findings correlated with human chorionic gonadotropin levels 总被引:2,自引:0,他引:2
Endovaginal sonography results were compared with quantitatively determined human chorionic gonadotropin (hCG) levels in 84 women referred for early pregnancy complications. Of the 27 with normal intrauterine pregnancies, an intrauterine gestational sac was prospectively identified in one of five cases (20%) in which hCG levels were below 500 IU/L (Second International Standard), four of five (80%) with hCG levels of 500-1,000 IU/L, and all 17 with hCG levels above 1,000 IU/L. In comparison, 17 of the 26 women with ectopic pregnancies (65%) had hCG levels greater than 1,000 IU/L, and none of the 26 had an intrauterine gestational sac. Endovaginal sonography demonstrated an adnexal mass and/or a gestational sac-like structure in 16 of the 17 cases (94%) in which hCG levels were above 1,000 IU/L, compared with only three of the nine (33%) with lower hCG levels (P less than .01). These findings indicate that an intrauterine gestational sac should be normally visualized with endovaginal sonography when the hCG level exceeds 1,000 IU/L, and that visualization of an extrauterine gestational sac and/or adnexal mass is significantly more likely in ectopic pregnancies when the hCG level exceeds 1,000 IU/L. 相似文献
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19.
Tuoheti Y Itoi E Pradhan RL Wakabayashi I Takahashi S Minagawa H Kobayashi M Okada K Shimada Y 《Journal of shoulder and elbow surgery / American Shoulder and Elbow Surgeons ... [et al.]》2005,14(5):535-541
The purpose of this study was to investigate the histopathology, including apoptosis, in the supraspinatus tendon with stage II subacromial impingement. Samples from the critical zone of the supraspinatus tendon were obtained from 5 patients with subacromial impingement syndrome and 10 autopsy cases without shoulder diseases as controls. Three-micrometer-thick sections were cut and stained with hematoxylin-eosin (H-E) for routine histologic examination. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method and single-stranded deoxyribonucleic acid (ssDNA) assay in which the frequency of the apoptotic cells was expressed by the apoptotic index. Control supraspinatus tendons showed normal morphology, whereas supraspinatus tendons from shoulders with impingement showed significant mucoid degeneration. Correspondingly, few apoptotic cells were observed in control tendons, whereas a large number of apoptotic cells were observed in the degenerative area of tendons from impingement shoulders. The apoptotic indices were significantly higher in the impingement shoulders (ssDNA, 18.84% +/- 1.75%; TUNEL, 24.92% +/- 2.79%) than in the control shoulders (ssDNA, 5.22% +/- 1.30%; TUNEL, 7.01% +/- 1.05%) (P = .04 for ssDNA and P = .017 for TUNEL). Mechanical impingement seems to cause tendon degeneration and apoptosis of the tendon cells in the supraspinatus tendon in stage II impingement. 相似文献
20.
To evaluate the susceptibility of rasH2 mice to N-bis(2-hydroxypropyl)nitrosamine (DHPN), a potent carcinogen targeting the lung, liver, thyroid, and kidney, male, 6-week old, rasH2 mice and wild-type littermates (non-Tg mice) were given DHPN in drinking water at 0, 20 or 200 ppm, and 0 or 200 ppm, respectively, for 26 weeks. The experiment using rasH2 mice given 200 ppm DHPN and non-Tg mice given 200 and 0 ppm DHPN was completed at 20 weeks, since mortality in these groups was remarkably increased due to hemangiosarcomas of the liver. Histologically, tumors developed in the lung and liver in both rasH2 and non-Tg mice treated with DHPN. In addition, proliferative lesions were observed in the forestomach, urethra, and excretory duct of salivary glands in rasH2 mice given 200 ppm DHPN. RT-PCR analysis showed no marked difference in expression of mRNAs for the transgene and the endogenous mouse ras gene between the whole lung tissue containing a neoplasm and normal lung tissue. Our results suggest that rasH2 mice are highly susceptible to DHPN, the target organs including the forestomach, salivary gland and urethra, which have not been found to develop tumors in previous long-term carcinogenicity studies of DHPN in rats and mice. 相似文献