AO苜蓿叶形支撑钢板治疗Pilon骨折

目的总结使用AO苜蓿叶形钢板治疗Pilon骨折的经验。方法分析我科自1996年3月-2001年6月用该方法治疗的23例病人的治疗效果：按Ruedi-Allgower分型：其中Ⅱ型18例，Ⅲ型5例，均采用切开复位，AO苜蓿叶形钢板内固定术。结果治疗后，临床疗效优良率82．6％，其中Ⅱ型达88．8％，Ⅲ型达60％。结论AO苜蓿叶形钢板治疗Pilon骨折，具有骨折稳定性可靠，有利于骨折愈合和早期关节活动的优点，Ⅲ型骨折的治疗方法有待进一步探讨。     

BIOEQUIVALENCE BETWEEN rhGH FOR RECONSTITUTION AND READY-TO-USE rhGH IN TWO LIQUID FORMULATIONS

Objective To evaluate the bioequivalence between recombinant human growth hormone （rhGH） for reconstitution, and two dosages of liquid formulation of rhGH [ （151U） 5mg or （301U） lOmg per 3ml ]. Methods The study drugs were tested in a randomized, single-blind and three-period crossover studies in 24 healthy male subjects. The three drugs were administered by subcutaneous injection at a dose of O. 21U/kg body weight. A continuous somatostatin infusion was given in order to suppress the secretion of endogenous GH. The ve- nous blood samples were drawn at different time points to test the serum concentration of GH. The pharmacokinetic parameters were analyzed by statistical methods. Results 90% confidence intervals （CI） of AUC0-24h among three products were all within 80% - 125% interval （ 103. 4% - 116. 5%, 105. 7% - 119. 6% and 91.9% - 103. 7%, respectively）, and the Cls of C,~ among three products were all within 70% - 143% interval （91.9% - 114. 0%, 103. 7% -127. 2% and 81.6% -97. 4%, respectively）. There was no statisitical difference of tmax among all the three products. Conclusion These data demonstrate that there is bioequivalence between rhGH for reconstitution and two liquid formulations of rhGH.     

活血化瘀中药联合血管内皮生长因子基因转移促进股骨头坏死处新生血管形成的实验研究

目的:观察活血化瘀中药联合血管内皮生长因子(VEGF)基因转移对促进股骨头缺血坏死处新生血管形成情况。方法:日本大耳兔40只,随机分为对照组、模型组、中药组、基因组和综合组。治疗8周后采用免疫组织化学方法观察股骨头滑膜VEGF阳性细胞率及数字减影血管造影股骨头血管数目改变情况。结果:模型组VEGF阳性细胞表达率减低,与对照组、基因组、综合组比较,差异有显著性意义(P<0.01),与中药组比较,有统计学差异(P<0.05),综合组VEGF阳性细胞表达率较高,与中药组及基因组比较,有统计学差异(P<0.05)。血管数目:在A区,各组与模型组比较,均无统计学差异;在B区,各组较模型组血管数目均有增加,对照组、基因组、综合组与模型组比较,有统计学差异(P<0.05)。综合组与基因组比较有统计学差异(P<0.05),中药组虽然血管数目较模型组多,但是无统计学意义。结论:活血化瘀中药及VEGF基因转移均可促进股骨头缺血坏死处局部新生血管形成和侧支循环的建立,尤以活血化瘀中药联合基因疗法效果为好,为临床应用活血化瘀中药联合基因疗法治疗股骨头缺血性坏死提供实验依据。     

硫酸锌对大鼠移植胰腺再灌注损伤的保护作用

Objective:To study the protective effects of the heat shock protein 70 （HSP70）induced by zinc sulfate on reperfusion injury following pancreaticoduodenal transplantation in rats.Methods :The homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was used. The ZnSO4 treated rats received the intravenous injection of Zn2+5 mins before and after operation at a dose of 5 mg/kg (Zn 1 group),10 mg/kg(Zn 2 group) and 15 mg/kg(Zn 3 group), and the control group with the same volume of saline. The tissue concentration of HSP70 was determined using Western Blot. In addition，blood sugar (BG) and serum concentration of amylase and lipase were examined 24h after transplantation, and the activity of myeloperoxidase (MPO) in the pancrease graft was measured at the same time. Histological observation was performed.Results:Light microscopic studies showed that histomorphological changes of pancreas in Zn 2 group and Zn 1 group were much less than those in control group and Zn 3 group. The value of BG and serum lipase and MPO in Zn 2 group ＜ Zn 1 group 相似文献   

退变腰椎间盘髓核细胞立体培养与单层培养的生物活性比较

目的：比较体外单层培养和旋转微载体立体培养人退变腰椎间盘髓核细胞的生物学活性指标,探讨更加有效的椎间盘髓核细胞体外培养方法.方法：对获取的腰椎间盘突出症患者的24个椎间盘按年龄分为A组（20～25 岁）、B组（26～30 岁）、C组（36～45 岁）及D组（＞45岁）,分别利用酶消化法进行单层细胞培养和旋转微载体立体培养系统进行立体培养,观察细胞生长形态,检测细胞生长曲线及速度、细胞生长活性、细胞分裂指数及胶原含量.结果：单层培养的髓核细胞贴壁后为多角形或梭形,伸出伪足;立体培养的细胞在微载体上呈梭形或不规则形,呈立体状生长.立体培养的细胞生长速度较快,1周内两种培养方法各时间点及各组间比较均具有统计学意义（P＜0.05）.立体培养的髓核细胞活性提高,随年龄增加细胞活性下降;指数生长期细胞分裂指数与单层培养相比有统计学意义（P＜0.05）;Ⅰ、Ⅱ型胶原含量与单层培养相比有统计学意义（P＜0.05）,A组分别与B组、C组及D组比较均有统计学意义（P＜0.05）.结论：旋转立体培养人退变椎间盘髓核细胞的活性保持良好,较单层培养能够大量、优质收集种子细胞,可用于椎间盘组织工程中种子细胞的研究.     

周围型肺腺癌CT、病理征象与肿瘤淋巴管密度及淋巴转移相关基因表达关系的研究

目的研究周围型肺腺癌的CT、病理征象与肿瘤组织VEGF-C、VEGFR-3表达及淋巴管生成的相关性。方法搜集40例经手术病理证实的肺腺癌,术前均行CT平扫及增强扫描,对肿瘤标本行SP免疫组化染色,根据其阳性表达计数阳性细胞数及淋巴管密度(LMVD),分析肺腺癌某些CT、病理征象与VEGF-C、VEGFR-3表达,LMVD值间的关系。结果淋巴结增大、转移及其它一些CT表现与VEGF-C、VEGFR-3过表达及LMVD值有密切相关性;肿瘤组织的VEGF-C、VEGFR-3表达与LMVD值亦有相关性。结论肺腺癌淋巴结增大等CT征象与VEGF-C、VEGFR-3表达及淋巴管生成具有相关性。     

Caspase-3反义寡核苷酸抗凋亡作用的研究

目的 研究caspase-3反义寡核苷酸对6-OHDA诱导大鼠中脑多巴胺神经元凋亡的保护作用.方法 向四组SD大鼠的中脑黑质部位注入反义、错义、正义寡核苷酸及NS,然后再注入6-OHDA,取中脑做连续切片,原位杂交及免疫组化检测黑质caspase-3的表达及TH的表达,原位末端标记法(Tunel)检测黑质细胞的凋亡.结果 反义组Tunel阳性细胞数为82±8.6,方差分析显示反义组阳性细胞数显著性低于其他各组(P＜0.05);反义组手术侧TH阳性细胞数为168.6±11.4,与对侧阳性细胞比值为63%±11.3%,显著性高于其余各组(P＜0.05).结论 有效阻断caspase-3的表达可减轻6-OHDA诱导的多巴胺神经元凋亡,caspase-3可作为保护性治疗帕金森病的靶点.     

Arthroscopic single-bundle reconstruction of posterior cruciate ligament with quadrupled hamstring tendon

Objective： To evaluate the technique and outcome of arthroscopic sipy, le-bundie reconstruction of posterior crudate ligament （PCL） with quadrupled hamstring tendon. Methods： From April 2001 to October 2004, 49 knees with PCL tears in 49 patients were verified with arthroscope in this department. Of them, 13 were combined with anterior cruciate ligament tears, 14 with disruptions of the posterolateral comer, 6 with ruptures of the posteromedial corner and medial collateral ligament, 9 with lateral meniscus tears, 5 with medial meniscus tears and 2 with popliteal vascular tears. All the damaged PCLs were reconstructed with single-bundie of autogenous quadrupled hamstring tendons under arthroscope. Biodegradable interference screws or blunt titanium interference screws were used for direct anatomic fixation of the reconstructed ligament. Results： After operation, no severe complications occurred at early stage in the 49 patients. All of them were followed up for 10-52 months with an average of 22. 0 months ＋ 10. 7 months. Lysholm score was remarkably improved from 30-60 （ mean： 47.96 ＋ 8. 16） preoperatively to 70-95 （ mean： 89. 08 ＋ 6. 10 ） at the last postoperative follow-up （ P 〈 0.01 ）. Furthermore, there was a significant improvement in International Knee Documentation Committee （IKDC） score from abnormal （ Grade C） in 10 knees and severely abnormal （Grade D ） in 39 preoperatively to normal （ Grade A ） in 20, nearly normal （ Grade B） in 24 and abnormal in 5 at the last follow-up. Of the 49 patients, 40 returned to the same activity level as before and 9 were under the level. Conclusions： Single-bundle reconstruction of PCL with quadrupled hamstring tendons has the advantage of minimal trauma in surgery and satisfactory outcome.     

汽车后送伤员附加装置隔振设计

采用钢丝绳隔振器设计了运输车后送伤员附加装置的隔振结构，并进行了隔振性能试验。5名受试者的试验结果表明，隔振装置的隔振性能良好，可衰减40％—70％的振动能量。本文为运输车后送伤员附加装置的隔振设计提供了一种新方法。     

双频谱指数在心脏瓣膜置换术中的观察

目的应用脑电双频谱指数(BIS)监测常规经验麻醉下瓣膜置换术病人的麻醉深度(意识水平),观察BIS在伤害性刺激时的变化.方法选择30例瓣膜置换术病人,采用芬太尼、咪唑安定、哌库溴铵及心血管活性药物对病人实施麻醉;术中监测并有专人分别记录入室(基础值)、诱导后、插管后、切皮后、锯胸骨时、缝胸骨时及术毕BIS和MAP,HR各参数值,观察不同阶段各参数的变化.结果诱导后各参数均明显降低,虽然切皮后、锯胸骨及缝胸骨等强刺激后MAP和HR未见统计学意义的显著改变,但BIS却显著增高(P＜0.01);全部病人锯胸骨时BIS均在60(67.28±8.97)以上,缝胸骨时63(68.46±8.70)以上,而无1例低于50者.结论该组瓣膜置换术病人麻醉偏浅,尤以锯胸骨、缝胸骨及术毕等时段为甚,表现为BIS值偏高,有知晓发生;知晓可发生在临床认为满意的麻醉中;应用BIS监测,可更加合理地评价麻醉深度.