全文获取类型
收费全文 | 55187篇 |
免费 | 5139篇 |
国内免费 | 3344篇 |
专业分类
耳鼻咽喉 | 589篇 |
儿科学 | 792篇 |
妇产科学 | 459篇 |
基础医学 | 5091篇 |
口腔科学 | 890篇 |
临床医学 | 6351篇 |
内科学 | 7305篇 |
皮肤病学 | 604篇 |
神经病学 | 2585篇 |
特种医学 | 2434篇 |
外国民族医学 | 12篇 |
外科学 | 6622篇 |
综合类 | 11349篇 |
现状与发展 | 21篇 |
一般理论 | 4篇 |
预防医学 | 4991篇 |
眼科学 | 898篇 |
药学 | 5583篇 |
61篇 | |
中国医学 | 3671篇 |
肿瘤学 | 3358篇 |
出版年
2024年 | 217篇 |
2023年 | 749篇 |
2022年 | 1891篇 |
2021年 | 2593篇 |
2020年 | 2073篇 |
2019年 | 1537篇 |
2018年 | 1610篇 |
2017年 | 1658篇 |
2016年 | 1486篇 |
2015年 | 2564篇 |
2014年 | 3044篇 |
2013年 | 3530篇 |
2012年 | 5098篇 |
2011年 | 5350篇 |
2010年 | 4025篇 |
2009年 | 3351篇 |
2008年 | 3810篇 |
2007年 | 3479篇 |
2006年 | 3223篇 |
2005年 | 2618篇 |
2004年 | 1948篇 |
2003年 | 1781篇 |
2002年 | 1357篇 |
2001年 | 873篇 |
2000年 | 711篇 |
1999年 | 440篇 |
1998年 | 242篇 |
1997年 | 227篇 |
1996年 | 211篇 |
1995年 | 206篇 |
1994年 | 152篇 |
1993年 | 112篇 |
1992年 | 121篇 |
1991年 | 128篇 |
1990年 | 110篇 |
1989年 | 99篇 |
1988年 | 80篇 |
1987年 | 83篇 |
1986年 | 68篇 |
1985年 | 67篇 |
1984年 | 76篇 |
1983年 | 54篇 |
1982年 | 51篇 |
1981年 | 51篇 |
1980年 | 48篇 |
1979年 | 45篇 |
1978年 | 47篇 |
1975年 | 39篇 |
1974年 | 39篇 |
1973年 | 36篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
311.
Kläning U Laursen TM Licht RW Kyvik KO Skytthe A Mortensen PB 《Journal of affective disorders》2004,81(2):141-145
BACKGROUND: A previous study demonstrated a higher rate of schizophrenia in dizygotic twins than in the general population, and a higher rate of schizophrenia in siblings of dizygotic twins than in siblings of monozygotic twins and singletons, pointing to a common genetic predisposition for dizygotic twinning and schizophrenia. The aim of the present study was to investigate whether these findings also apply to bipolar disorder. METHODS: Through record linkage between The Danish Twin Register, The Danish Psychiatric Central Register and The Danish Civil Registration System, the rate of bipolar disorder (diagnosed for the first time during admission to hospital) in dizygotic and monozygotic twins was compared with the rate in singletons, and the rate in siblings and parents of twins was compared with the rate in siblings and parents of singletons. RESULTS: The rate of bipolar disorder was the same in dizygotic twins, monozygotic twins and singletons as well as for parents and siblings of dizygotic twins, monozygotic twins and singletons. LIMITATIONS: The study is a register-based study, only including hospitalized patients. CONCLUSION: This study shows that there is an equal rate of bipolar disorder in twins and in singletons. Assuming that DZ twinning is under some genetic influence, a differential relationship between schizophrenia and DZ twinning on one hand and bipolar disorder and DZ twinning on the other hand may suggest differences in the genetic basis of the two diseases. The finding that the rate of bipolar disorder in monozygotic twins is the same as the rate of bipolar disorder in singletons supports studies finding no association between bipolar disorder and obstetric complications. 相似文献
312.
Conclusions NT antibodies to LI virus were found in the sera of Swedish cows from many parts of the country although only exceptionally in the north. The prevalence of positive specimens increased markedly towards the southeast. This geographic distribution apparently fits well with the occurrence of ticks in the country. An analysis, now in progress, of RSSE-LI antibodies in human cases of meningoencephalomyelitis seems to indicate a closely similar distribution of the human RSSE infections. It is thus obvious that the screening of cow sera for LI antibodies provides reliable information as to endemic regions of RSSE-LI-like viruses. There was nothing to suggest that nonspecific reactions occurred or impaired the results; in fact the very uneven geographic distribution of positive specimens, of high as well as low titers, should argue for specificity of the reaction.Aided by a grant from the Swedish National Association against Poliomyelitis.The skilful technical assistance of MissSiri Austrin, MissIngrid Lövhvist, MissBrita Gustafson and Mr.Nils Lundh is gratefully acknowledged. 相似文献
313.
Johannsson OT Staff S Vallon-Christersson J Kytöla S Gudjonsson T Rennstam K Hedenfalk IA Adeyinka A Kjellén E Wennerberg J Baldetorp B Petersen OW Olsson H Oredsson S Isola J Borg A 《Laboratory investigation; a journal of technical methods and pathology》2003,83(3):387-396
A human tumor xenograft (L56Br-X1) was established from a breast cancer axillary lymph node metastasis of a 53-year-old woman with a BRCA1 germ-line nonsense mutation (1806C>T; Q563X), and a cell line (L56Br-C1) was subsequently derived from the xenograft. The xenograft carries only the mutant BRCA1 allele and expresses mutant BRCA1 mRNA but no BRCA1 protein as determined by immunoprecipitation or Western blotting. The primary tumor, lymph node metastasis, and xenograft were hypodiploid by DNA flow cytometry, whereas the cell line displayed an aneuploidy apparently developed via polyploidization. Cytogenetic analysis, spectral karyotyping, and comparative genomic hybridization of the cell line revealed a highly complex karyotype with numerous unbalanced translocations. The xenograft and cell line had retained a somatic TP53 missense mutation (S215I) originating from the primary tumors, as well as a lack of immunohistochemically detectable expression of steroid hormone receptors, epidermal growth factor receptor, human epidermal growth factor receptor 2 (HER-2), and keratin 8. Global gene expression analysis by cDNA microarrays supported a correlation between the expression profiles of the primary tumor, lymph node metastasis, xenograft, and cell line. We conclude that L56Br-X1 and L56Br-C1 are useful model systems for studies of the pathogenesis and new therapeutic modalities of BRCA1-induced human breast cancer. 相似文献
314.
QING HUA ZOU BO QING LI JIAN ZHONG ZHANGNational Institute for Communicable Disease Control Prevention Chinese Center for Disease Control Prevention Beijing P.R.China 《中华微生物学和免疫学杂志(英文版)》2005,3(2):83-88
Cholera ,as one of the most severe epidemic dis-eases inthe world,can occurin a short time ,andspread quicklyto a wide region.Until 1992 ,onlyV.choleraeserogroup O1 was recognized as thecause of epidemic cholera . However in October1992 ,a major outbreak of a cholera-like illnesscaused by a newserogroupstrain ofV.choleraee-mergedinIndia and Bangladesh.In contrast to allpreviously reported non-O1 strains , which induceonly sporadic cases of human diarrhea without epi-demic potential ,the new… 相似文献
315.
对 PHA、抗 CD3单克隆抗体 RW2—8C8、胸腺肽、抗 CD2与抗 CD3等对人 T 淋巴细胞的增殖现象及动力学进行了观察,除抗 CD3与抗 CD2单抗联合应用为抑制效应外,前三者均呈正效应且动力学完全一致.在此基础上,应用反义 CD2mRNA 探针检测了 mRNA 在激活24小时以后的变化,发现 T 细胞活化者均显示 mRNA 量呈上升;反之则不见变化或低于未激活 T 细胞对照组水平.本研究证明了小牛胸腺肽有激活 T 细胞和提高 mRNA 水平的作用,为首次报导.本文对上述在基因水平上观察的结果进行了讨论. 相似文献
316.
中国汉族雄激素过多症女性21-羟化酶缺陷症携带者基因检测 总被引:3,自引:0,他引:3
目的 初步了解中国汉族女性雄激素过多症患者中2 1-羟化酶缺陷症(2 1- hydroxylasedeficiency,2 1- OHD)携带者发生率,探讨促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)兴奋试验结果与基因突变检测结果的相关性。方法 82例汉族女性雄激素过多症患者及14名健康女性进行ACTH兴奋试验,并应用PCR扩增产生限制性酶切位点方法检测已知的9个2 1- OHD常见突变位点。结果 雄激素过多组(n=82 ) F0 显著高于正常对照组(P<0 .0 1) ;17- OHP0 及17- OHP6 0 也显著高于对照组(P<0 .0 1) ,而F6 0 差异没有统计学意义(P>0 .0 5 )。比较17- OHP净增值及17- OHP净增值/ F净增值,雄激素过多组也均显著高于正常对照组(P<0 .0 1)。正常对照组未检测出细胞色素P4 5 0 (cytochrome P4 5 02 1,CYP2 1)基因突变。发现雄激素过多组4例CYP2 1基因突变携带者(4/ 82 ,4 .9% ) ,分别携带V2 81L(2例) ,i2 g及Q318X(各1例) ,携带者的ACTH兴奋试验结果与正常对照及未检出突变的雄激素过多症患者的结果存在一定的交叉。结论 82例汉族雄激素过多症女性中2 1- OHD携带者为4例,占4 .9%。ACTH兴奋试验不能用以发现携带者,应进行基因检测确定。 相似文献
317.
目的 利用寡核苷酸芯片平行分析乙肝病毒表面抗原区S、前核心抗原pre C区、X区、聚合酶P区 12个已知的突变位点。方法 针对S、pre C、X、P区的 12个突变位点 ,设计位于乙肝病毒反义链的 2 4条寡核苷酸探针和两对PCR引物 ,探针长度为 14~ 18bp ,其 5′端连有氨基己烷和T15间隔子 ,合成后经点样仪点到醛基化玻片上。两对PCR引物分别用于扩增S与P区内的 5个突变位点和X、前C区内的 7个突变位点 ,其中上游引物含有荧光标记。不对称PCR扩增所得到的荧光标记的单链DNA与寡核苷酸阵列杂交、清洗后经扫描分析实验结果。结果 在对 12个乙肝阳性样品前核心抗原C和X区的突变检测中 ,存在 176 2A→T ,176 4G→A联合突变的有 2例、1896G→A突变的 3例、同时存在 176 2A→T、176 4G→A联合突变和 1896G→A突变的 1例、未存在任何突变的样品 6例。在 12个乙肝阳性样品的表面抗原S的突变检测中 ,未发现有突变出现。部分样品的随机测序结果与寡核苷酸阵列杂交结果一致。结论 寡核苷酸芯片适于快速、平行、大量检测突变 相似文献
318.
目的研究1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者CYP17A1基因突变特点,并结合患者的临床表现与基因突变类型初步探讨P450C17酶蛋白的结构与功能的关系。方法收集1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者的临床资料及其亲属血标本,提取基因组DNA,设计7对引物扩增CYP17A1基因的8个外显子及外显子与内含子的连接区域,琼脂糖凝胶电泳鉴定PCR产物,产物胶回收后直接做为DNA双链模板测序。DNA双链模板不一致的PCR产物经克隆后测序。测序结果在核苷酸序列数据库进行比较分析。结果患者CYP17A1基因突变检测结果为5994-5995delAT/7541C>T复合杂合子。这两种突变均未见报道。推测5994-5995delAT导致I259H,274X,突变形成的截短蛋白质缺少血红素结合区域,因此是没有功能的;而通过人类P450C17酶计算机模型分析显示7541C>T导致的A398V远离酶的活性中心,推测突变可能使酶的活性减弱,而不是完全地丧失。患者临床表现为有自发不规则月经及轻度高血压、低血钾,结合激素测定结果提示肾上腺和性腺保留部分功能。因而患者的基因型与其临床表型是一致的。结论应进行突变P450C17酶的功能学研究来进一步明确结构改变对功能的影响。 相似文献
319.
目的 探讨终末期肝病模型(MELD)评分评估肝储备功能在原发性肝癌合并肝硬化脾功能亢进中确定手术适应证的应用价值.方法 对2001年1月至2007年1月间行肝癌切除联合脾切除(联合术)治疗的40例原发性肝癌合并肝硬化脾功能亢进患者的临床资料进行回顾性分析.通过MELD评分与Child-Pugh分级比较,结合临床资料及术后并发症分析,确定这一方法在评估肝储备功能中的作用.结果 同一Child-Pugh分级的患者MELD评分结果并不一致,在各级别间有交错现象.术后发生肝功能衰竭组(6例)的MELD评分均值为(24.6±6.6).未发生肝功能衰竭组(34例)的MELD评分均值为(16.3±8.5),差异有统计学意义(P<0.05).根据MELD评分分为A组16例(MELD评分<10),B组17例(MELD评分10-20),C组7例(MELD评分>20).A组术后肝功能衰竭发生率为0,B组为11.8%(2/17),C组为57.1%(4/7),差异有统计学意义(P<0.05).根据Child-Pugh分级分为Ⅰ级26例,Ⅱ级14例.Ⅰ级术后肝功能衰竭发生率为15.4%(4/26),Ⅱ级为14.3%(2/14),差异无统计学意义(P>0.05).结论 MELD评分能够较为客观地反映肝储备功能,对外科术式的选择、手术时机的确定有一定的参考作用. 相似文献
320.
Yongchuan Chai Yu Wang Hua Qian Bo Yang Chao Wu Jiusheng Chu Tao Yang 《American journal of medical genetics. Part A》2019,179(2):243-248
Waardenburg syndrome (WS) is a genetic disorder characterized by hearing loss and pigmentary abnormalities with variable penetrance. Though heterozygous mutations in MITF are a major cause for Waardenburg syndrome type 2 (WS2), homozygous mutations in this gene and the associated phenotype have been rarely characterized. In this study, we identified a novel p.R223H mutation in MITF in a Chinese Han family with variable WS features. Both parents carried a heterozygous p.R223H mutation. They had normal hearing, and premature greying of the hair is their only pigmentary abnormality. In contrast, their two children both carried a homozygous p.R223H mutation and had classic WS features including profound hearing loss, heterochromia irides and marked pigmentary abnormalities in hair and skin. Interestingly, the two affected children also have persistent chronic constipation since the neonatal period, symptoms suggestive of Waardenburg syndrome type 4 (WS4). Our study revealed a likely association between homozygous mutations in MITF and WS4, which implies a dosage effect for the underlying pathogenesis mechanism. 相似文献