表皮生长因子受体在颊粘膜鳞状细胞癌中的表达及其临床病理学意义

表皮生长因于受体（EGFR）是一单跨膜糖蛋白，与细胞的增殖、转化有关。本文采用免疫组化ABC法检测8例正常颊粘膜上皮及40例颊粘膜鳞癌中EGFR的表达状况，结果显示：EGFR在颊癌组织中呈异质性表达，其阳性率明显高于正常颊粘膜组织（P＜0．05）；EGFR表达状况与颈淋巴结转移及预后间存在相关关系（P＜0．05）。EGFR（＋）表达者多数伴有颈淋巴结转移，预后差。以上结果说明，EGFR在颊癌组织中的表达水平，可作为评估颊癌细胞增殖状态，颈淋巴结转移情况及患者预后的一项参考指标。     

茶多酚对+Gz过载的颞下颌关节影响分析

目的：观察茶多酚对持续性高正加速度（＋Gz）加载颞下颌关节的影响。方法：雄性Wistar大鼠随机分成5个不同处理条件的实验组，每组12只。A组为空白对照。B组地面固定5min。C 固定于离心机转臂上，俯卧位，头向轴向，＋Gz离心5min。D组体位同C组，＋10Gz离心30s，间隔时＋1Gz离心60s，连续每天5次，每周4天，共3周，E组实验参数同D组，并于＋Gz离心前1h灌胃给予茶多酚（200mg.kg^-1)，其余各组给予等量蒸馏水，通过光镜，扫描和透射电镜观察比较组颞下颌关节的组织形态学变化情况，结果：A、B、C三组颞下颌关节未见异常；D组颞下颌关节表面凝胶状物质缺失，胶原纤维断裂，成纤维细胞线粒体变性；E组颞下颌关节表面凝胶状物质变薄但完整，胶原纤维断裂，成纤维细胞线粒体较正常，结论：茶多酚能在一定程度上保护凝胶状物质和成纤维细胞来对抗持续性高正加速度对颞下颌关节的损伤。     

CVP动脉灌注联合手术治疗晚期上颌窦鳞癌的疗效(附39例分析)

目的 :探讨CVP方案行颞浅动脉灌注术前诱导化疗联合手术治疗 39例晚期上颌窦癌患者的可行性。方法 :39例T4患者采用卡铂、足叶乙甙、平阳霉素诱导化疗 ,然后施行上颌骨扩大切除加同侧根治性颈淋巴清扫术。结果 :所有患者均可耐受化疗 ,化疗疗效评价均为部分缓解。术后失访 5例 ,随访资料完整 34例 ,占87%。所有病例无原发灶复发。其中 4例死于远位转移 ,分别为术后第 5月、第 7月、第 8月和第 12月。 30例无瘤生存。 5年以上 17例 ,3年以上 6例 ,1年以上 4例 ,不足 1年 3例。结论 :CVP颞浅动脉灌注术前诱导化疗联合手术治疗晚期上颌窦癌 ,是提高远期疗效的有效方案。合理的术后化疗及免疫治疗是减少术后远位转移 ,提高远期生存率的重要因素。也是今后需要高度重视的问题     

应用牵张成骨技术行牙槽嵴增高术的研究进展

牵张成骨 (distractionosteogenesis,DO)指在骨缝处或在截开的骨段用牵张装置按一定的速度和频率牵开 ,因此产生的骨间隙中形成新骨 ,从而达到使骨延长或增宽的目的。该技术用于四肢长骨已有近百年历史 ,在颅面部治疗颌骨缺损、畸形及阻塞性睡眠呼吸暂停综合征等领域也获得了成功。近年来 ,用牵张成骨技术增高牙槽嵴逐渐成为研究热点 ,现综述如下。一、牵张成骨技术与传统牙槽嵴增高术的比较牙槽嵴增高术分相对增高及绝对增高两种 ,前者指唇颊沟加深或牙槽嵴延伸术 ,后者指牙槽嵴增高术。唇颊沟加深术通过改变黏膜及肌附着的位置 ,使牙槽嵴…     

Clinical application of angiography of oral and maxillofacial hemangiomas. Clinical analysis of seventy cases

Angiographic findings in seventy cases of maxillofacial hemangioma are reported. The tumors have been divided into four types: (1) plexiform hemangioma, (2) cavernous hemangioma with venous drainage, (3) cavernous hemangioma without venous drainage, and (4) the so-called "diffuse type" of cavernous hemangioma. The angiographic features of the four types are described, and clinical application is evaluated. The angiograms demonstrate the location, extent, blood supply, and drainage of the tumors and show whether they communicate intracranially, thus providing valuable criteria for planning and determining the mode of treatment.     

Role of type IV collagene and type IV collagenase in the invasion and metastasis of salivary adenoid cystic carcinoma]

Using immunohistochemical ABC method, the authors studied the distribution of type IV collagen and type IV collagenase in 25 cases ACC. The result showed type IV collagen was found in vascular and epithelial basement membrance, and in partly cyst of sieve-like and tubular pattern, and in cytoplasm of tumor cell. Type IV collagenase was detected in membrance and cytoplasm of tumor cell. Expression of type IV collagen and type IV collagenase were correlated with pathologic type, TNM stage and metastasis. Much positive staining of type IV collagen was found in sieve-tubular pattern, early stage of TNM and without metastasis, but much positive staining of type IV collagenase was found in solid pattern, later stage of TNM and metastasis. The result suggested that the loss of type IV collagen and over-expression of type IV collagenase may be markers of malignance of ACC.     

Expression and possible immune-regulatory function of ghrelin in oral epithelium

Originally found in stomach mucosa, ghrelin is a peptide appetite hormone that has been implicated as an immuno-modulatory factor. Ghrelin has also been found in salivary glands and saliva; however, its expression patterns and biological properties in the oral cavity remain unclear. Therefore, we investigated the expression patterns of ghrelin in saliva, gingival crevicular fluid (GCF), and gingival tissue, as well as its in vitro effects on IL-8 production by TNF-α or LPS-stimulated oral epithelial cells. In the clinical samples obtained from 12 healthy volunteers, the concentration of ghrelin in GCF remarkably exceeded that detected in saliva. The expression of ghrelin mRNAs and growth hormone secretagogue (GHS) receptors could be detected in human oral epithelial cells. Immunohistochemical analysis revealed the expression of ghrelin in gingival epithelium, as well as in fibroblasts in the lamina propria. Ghrelin increased intracellular calcium mobilization and cAMP levels in oral epithelial cells, suggesting that ghrelin acts on epithelial cells to induce cell signaling. Furthermore, synthetic ghrelin inhibited the production of IL-8 from TNF-α or LPS-stimulated oral epithelial cells. These results indicate that ghrelin produced in the oral cavity appears to play a regulatory role in innate immune responses to inflammatory infection.