脊柱骨折的CT诊断

目的:探讨西藏高原地区脊柱骨折的CT影像表现和诊断价值。方法:对45例脊柱外伤的CT扫描影像表现进行了回顾性的分析。男38例,女7例,年龄21～72岁。结果:CT能够准确地发现脊柱骨折的部位,三柱的损伤情况,椎管狭窄的程度,椎体骨折的类型。本组病例中,颈椎骨折的发生率最低,胸腰椎骨折的发生率最高。结论:在脊柱外伤中CT作为重要的首选检查方法,可快速准确地作出诊断,为临床提供治疗依据。     

长沙市农村老年人自杀死亡漏报调查

目的 掌握农村老年人自杀死亡漏报的一手资料，为提高农村老年人自杀研究的可靠性提供依据。方法 采取分层整群抽样，随机抽取长沙东部的浏阳市和西部的望城县为研究现场，将二县(市)所有乡(镇)按人均年收入分为经济状况好、中、差三个层次，从每层中随机抽取1～2个乡(镇)共8个乡(镇)作为研究点，以其全部145个行政村1996～2000年自杀死亡的60岁及以上老年人为对象，调查和分析自杀死亡老年人的漏报情况。结果农村老年人群是自杀死亡漏报率为32．84％。结论 农村老年人自杀死亡存在较为严重的漏报现象．     

网络成瘾问题研究现状及展望

网络成瘾作为一个日益突出的社会问题,已引起不少地区或部门的重视,本文将从网络成瘾的诊断及类型,网络成瘾的原因、危害以及对成瘾者的综合防治等几方面做系统阐述.     

胆石症病人胆囊黏膜中幽门螺杆菌的免疫组化检测及意义

目的：研究幽门螺杆菌（H.pylori)感染与胆石形成的关系。方法：对50例胆石症病人中血清及胆汁幽门螺杆菌IgG阳性的29例手术切除胆囊标本，采用常规Giemsa染色及特殊幽门螺杆菌免疫组化染色进行观察。结果：29例血清及胆汁幽门螺杆菌IgG阳性胆囊黏膜标本中Giemas检出23例（79．3％），6例为阴性；H.pylori免疫酶标记3例为阳性（10．34％），26例为阴性。Giemsa染色阳性切片中，在黏膜表面上皮，腺体上皮表面及腺腔的黏液中，可发现染成蓝色细菌。免疫组化阳性切片中，在同样部位发现呈阳性反应的着棕黄色菌体。细菌球形菌明显多于杆状菌。结论：在胆囊黏膜组织中可发现幽门螺杆菌样菌。     

Posttransplantation cyclosporine-induced lymphoproliferative disorders: clinical and radiologic manifestations

Fifteen allograft transplant recipients acquired lymphoproliferative disorders after immunosuppressive therapy with cyclosporine and steroids. Many of these lymphoproliferative disorders regressed or disappeared completely after reduction of cyclosporine dose. This disease has several aspects that distinguish it from usual posttransplantation lymphomas that occur with regimens that do not contain cyclosporine. The time course from transplantation to onset of lymphoma is relatively short, with an average of approximately 8 months. Organs show a wide spectrum of abnormalities typical of other immunosuppression-associated lymphomas, but there is unique sparing of the central nervous system. The tumor is also unique in that it responds to a decrease in the cyclosporine dose.     

噻嗪类利尿药引起外源性光敏性皮炎临床研究

目的 :探讨噻嗪类利尿药引起光敏性皮炎的临床特点及治疗。方法 :停用含有噻嗪类的光敏性药物并避光 ,同时予抗组胺药物口服 ,类固醇霜剂外用对症处理。结果 :10例在 14～ 2 0d后皮损基本控制 ,2例患者皮损时轻时重 ,随访 2个月疗效不佳。结论 :噻嗪类利尿药主要导致皮肤的光敏性反应 ,若不及时停药避光则有可能诱发持续性光敏反应。     

浅议定向运动的绿色教育特征

从绿色教育的起源与发展出发，揭示绿色教育具有环境教育、可持续发展观、和谐性、人本主义教育观的教育特征。阐述定向运动的知识结构、教学方法、评价方法具有多种绿色教育特征，表明定向运动是符合当代绿色教育理念的体育项目。     

Identification of B cell epitopes at the C-terminus of latency-associated nuclear protein of the kaposi's sarcoma-associated herpesvirus

The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus (KSHV) plays a key role in the induction of cell transformation, maintenance of viral episome, and modulation of immune response in human. To identify the presence of B cell epitopes within C-terminus of LANA and to characterize the monoclonal antibodies (MAbs) against this protein, we expressed the C-terminal region at aa 794-1000 of LANA (pLANA-C) in Escherichia coli as a fusion protein. KSHV-positive human sera were able to recognize the recombinant LANA-C in the Western blot analysis and ELISA. Mapping of antigenic epitopes of pLANA-C by KSHV-positive human sera revealed two B cell antigenic epitopes located at aa 846-854 and aa 794-822. The MAb 3F11 recognized a region between at aa 840 to 846 of LANA and exhibited a strong and specific binding to both pLANA-C and native viral LANA. These findings showed that pLANA-C and MAb 3F11 could be used for the detection of KSHV antibodies in human sera and for the advanced study of biological functions of LANA.     

Activation of the UNC5B receptor by Netrin-1 inhibits sprouting angiogenesis

Netrins are secreted molecules with roles in axonal growth and angiogenesis. The Netrin receptor UNC5B is required during embryonic development for vascular patterning, suggesting that it may also contribute to postnatal and pathological angiogenesis. Here we show that unc5b is down-regulated in quiescent adult vasculature, but re-expressed during sprouting angiogenesis in matrigel and tumor implants. Stimulation of UNC5B-expressing neovessels with an agonist (Netrin-1) inhibits sprouting angiogenesis. Genetic loss of function of unc5b reduces Netrin-1-mediated angiogenesis inhibition. Expression of UNC5B full-length receptor also triggers endothelial cell repulsion in response to Netrin-1 in vitro, whereas a truncated UNC5B lacking the intracellular signaling domain fails to induce repulsion. These data show that UNC5B activation inhibits sprouting angiogenesis, thus identifying UNC5B as a potential anti-angiogenic target.