紧密连接蛋白Claudin-3与三阴性乳腺癌患者临床特征的相关性

目的:探讨紧密连接蛋白Claudin-3与三阴性乳腺癌患者临床特征的相关性。方法:自2012年3月至2017年3月,收集我院收治的三阴性乳腺癌患者108例,行乳腺癌根治术,术中取乳腺癌标本和癌旁标本,检测组织中紧密连接蛋白Claudin-3表达情况,分析紧密连接蛋白Claudin-3与患者临床病理参数的相关性。对患者随访1年,分析术后1年复发患者与不复发患者组织中紧密连接蛋白Claudin-3的差异。结果:乳腺癌组织和癌旁组织中紧密连接蛋白Claudin-3表达无统计学差异(P=0.331)。与紧密连接蛋白Claudin-3阴性的患者相比,紧密连接蛋白Claudin-3阳性的患者TNM分期为Ⅲ期的比例显著增高(52.46% vs 29.79%,P=0.018),淋巴结转移比例增高(55.74% vs 29.79%,P=0.007),术后复发率显著增高(26.23% vs 10.64%,P=0.042)。结论:紧密连接蛋白Claudin-3与淋巴结转移和TNM分期有关,对预测患者术后复发具有一定价值。     

非小细胞肺癌survivin和Flk1表达及关系分析

目的:观察survivin蛋白和血管内皮细胞生长因子(VEGF)受体FIk1在不同病理类型及不同临床分期的非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)中的表达规律并探讨其相互关系。方法:采用临床NSCLC患者病理标本进行研究,NSCLC组为56例非小细胞肺癌肿瘤病理标本,患者临床资料分类中,临床Ⅰ期15例、Ⅱ期28例、Ⅲ期13例,病理分级分类中高分化肿瘤16例、中分化肿瘤22例、低分化肿瘤18例,对照组为18例癌旁组织。采用SABC法进行survivin蛋白和FIk1蛋白表达的免疫组织化学检测,分析染色结果确定阳性和阴性。结果:56例NSCLC中44例survivin表达阳性,survivin阳性率为78.6%(44/56),显著高于癌旁组。survivin阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。56例NSCLC中42例FIk1表达阳性,占75.0%(42/56),显著高于癌旁组。FIk1阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。NSCLC中survivin与FIk1阳性表达显著相关。结论:Survivin和FIk1共同高表达与NSCLC发生发展及浸润转移有显著关系。     

洋葱伯克霍尔德菌群(Bcc)及干扰菌在不同增菌培养基中的生长研究

目的：考察Bcc以及Bcc与干扰菌的混合物在不同的增菌培养基中能否生长和增殖;探讨不同的 Bcc菌种对增菌培养基的营养需求和抗生素的耐受是否存在差异。方法：将Bcc的代表菌株、干扰菌以及 Bcc与干扰菌的混合物分别接种至TSB、10倍稀释的TSB、添加10 mg·L-1庆大霉素的TSB和添加250000 U·L-1多粘菌素B的TSB中培养,划线分离至选择性培养基上观察结果,并对可疑菌落进行分离和鉴定。结果：只接种Bcc的4种增菌培养基均能使之良好地增殖。低量的Bcc(约102 cfu)与高量的干扰菌 (104 cfu)的混合接种,除了神秘伯克霍尔德菌CMCC(B)23010外,4种增菌培养基能使Bcc增殖并被检出。结论：添加抗生素的TSB与未添加抗生素的相比没有表现出明显的优势。TSB可以作为Bcc检验时的增菌培养基使用。生长缓慢且活力弱的Bcc菌株在特定条件下有可能会被漏检。     

Assessment of endometrial receptivity during implantation window in women with unexplained infertility

Abstract

This study aimed to assess the endometrial receptivity during implantation window in women with unexplained infertility. A prospective study recruited 168 women with unexplained infertility and 169 fertile women. Ultrasonic parameters and biomarkers in the uterine fluid were detected. The endometrial vascularization index (VI), flow index (FI) and vascularization flow index (VFI) were significantly higher in fertile women as compared with unexplained infertile women, and the integrin αvβ3, vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α), and leukemia inhibitory factor (LIF) levels in uterine fluid were significantly higher in fertile women. The biochemical pregnancy rate, clinical pregnancy rate, and ongoing pregnancy rate in fertile women were 20.12%, 18.34%, and 17.75%, respectively, which were significantly higher compared with unexplained infertile women (7.14%, 5.36%, and 4.17%, respectively). Endometrial thickness (ET), endometrial volume (EV), VI, FI, and VFI measured by ultrasound, and the integrin αvβ3, VEGF, TNF-α, and LIF levels in uterine fluid were all significantly higher in pregnant women as compared with nonpregnant women. The best parameters of ultrasonic indicators for predicting endometrial receptivity in women with unexplained infertility were FI(AUC = 0.894, sensitivity 93.8%, and specificity 83.1%). Integrin αvβ3 had the best predictive value for endometrial receptivity among biomarkers in the uterine fluid (AUC = 0.921, sensitivity 96.7%, and specificity 89.5%). Women with unexplained infertility present declined endometrial receptivity. Endometrial ultrasonic parameters detected by three-dimensional power Doppler and biomarkers in the uterine fluid may be effective indicators to predict endometrial receptivity.     

成年功能性构音障碍患者辅音错误类型及方式分析

目的 探讨成年功能性构音障碍患者辅音发音错误的特点,为制定语音训练方案提供依据.方法以42例成年功能性构音障碍患者为研究对象,借助计算机语音工作站对患者进行语音评估,对其辅音的发音错误类型及方式进行分析.结果42例成年功能性构音障碍患者主要的辅音发音错误类型为置换、扭曲,其次为脱落.按发音部位,错误辅音依次为舌尖后音(39例,92.86%)、舌尖前音(31例,73.81%)、舌面音(22例,52.38%)、舌根音(19例,45.24%)、舌尖中音(18例,42.86%)、唇齿音(6例,14.29%)及双唇音(6例,14.29%);辅音错误频率由高至低依次为/sh/、/zh/、/ch/、/r/、/z/、/c/、/s/、/q/、/x/、/j/、/g/、/k/、/h/、/t/、/l/、/d/、/f/、/p/.辅音错误方式有:舌前音化(22例,52.38%)、非送气化(12例,28.57%)、侧化构音(12例,28.57%)、辅音脱落(9例21.42%)、舌后音化(8例,19.05%)、舌面音化(4例,9.52%)、双唇音化(4例,9.52%)、唇齿音化(3例,7.14%).结论成年功能性构音障碍患者的辅音发音错误类型主要是置换和扭曲;错误辅音主要为舌尖后音、舌尖前音、舌面音、舌根音、舌尖中音;辅音发音错误方式主要为舌前音化、非送气化、侧化构音、辅音脱落.     

Exploring the mechanism of Buyang Huanwu decoction in the treatment of lumbar disc herniation based on network pharmacology and molecular docking

Buyang Huanwu decoction (BYHWD), as one of the traditional Chinese medicine formulas, is widely used in the clinical treatment of lumbar disc herniation (LDH) with curative effect. It has the characteristics of multi-component, multi-target, and mutual synergy, but the mechanism of action is often unclear. It needs some research to explore the molecular mechanism of BYHWD in the treatment of LDH based on network pharmacology and molecular docking. Screen the active compounds of BYHWD and predict drug-related gene/protein targets, which could determine the specific target of BYHWD in the treatment of LDH. Construct the “Drugs-Compounds-Targets” network and search for the core targets. Use Gene Ontology functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, and molecular docking verification to explore the possible molecular mechanism. Eighty-two effective compounds and 666 targets of BYHWD, 187 targets for LDH treatment, and 20 core candidate targets were excavated. A total of 3414 entries were identified by Gene Ontology enrichment analysis, 173 related signal pathways were identified by Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and 5 core compounds were identified by molecular docking, which had a good affinity with core genes STAT3, JUN, AKT1, MAPK1, RELA, and PIK3CA. BYHWD may play the role of analgesic and improving function by synergistic anti-inflammatory and analgesic compounds, regulating cell metabolic differentiation, regulating immunity, and anticoagulation. BYHWD in the treatment of LDH may play a role in analgesia and improve function through multiple signaling pathways, including PI3K-Akt, mitogen-activated protein kinase, tumor necrosis factor, and interleukin-17. The PI3K-Akt signaling may be one of the key mechanisms.     

复方芪芍合剂的质量控制

目的建立复方芪芍合剂的质量控制方法。方法应用薄层色谱法对复方芪芍合剂中黄芪、赤芍进行定性鉴别,并用紫外分光光度法对复方芪芍合剂中总皂苷进行含量测定。结果测得复方芪芍合剂中总皂苷量平均为0.436 3 g.L-1,RSD为8.59%,加样回收率为88.32%。结论本方法专属性强,操作简便,结果准确,可用于复方芪芍合剂的质量控制。     

降压治疗对老年高血压及合并脑梗死患者心率变异性的影响

目的研究不同程度降压对老年高血压及合并腔隙性脑梗死患者心率变异性的影响,探讨高血压不同病理状态下HRV变化的差异。方法选择老年原发性高血压(高血压组)及合并腔隙性脑梗死(腔梗组)患者各33例为观察对象;同期健康老年人自愿者(健康对照组)27例,测定降压治疗前后不同血压水平下心率变异性参数的变化。结果降压治疗前高血压并脑梗组和高血压组心率变异性的各时域指标均较正常组明显减小,其中高血压并脑梗组较高血压组减小更明显,但2组比较仅SDNNI和RMSSD差异有统计学意义(P<0.05)。降压治疗后无论高血压组或高血压并脑梗组各时域指标均有不同程度的增高,第2次降压较第1次降压后各组各时域指标增高更明显。第1次降压后和降压前比较,各时域指标仅高血压组SDANNI和PNN50差异有统计学意义(P<0.05);第2次降压后和降压前比较,高血压组SDNN、SDANNI和PNN50差异有统计学意义(P<0.05),高血压并脑梗组SDNN和PNN50亦有显著差异;第2次降压后与第1次降压后比较,仅SDNN有显著差异(P<0.05)。结论高血压及合并腔隙性脑梗死患者心率变异性均有不同程度降低,合理降压治疗可部分提高不同病理阶段高血压患者心率变异性,改善自主神经功能。     

Clinical Features in Patients with Xq23 Microdeletion: A Case Report and Literature Review

Xq22.3-q23 microdeletion is a rare genomic disorder. The purpose of this study was to emphasize the correlation between clinical phenotype and genotype of proximal deletion on chromosome Xq22.3-q23. A 5 years old boy had a 671 KB microdeletion on Xq23 by chromosomal microarray analysis, including AMMECR1 and CHRDL1 genes. He presented with microsomia, midface hypoplasia, right kidney dysplasia and mildly motor retardation, which have not previously been reported in relation to Xq23 deletion. To the best of our knowledge, this is the first case with Xq23 microdeletion. A total of nine cases with microdeletion at Xq22.3-q23 affecting AMMECR1 and two cases with CHRDL1 mutation were reviewed. This review showed that Xq23 microdeletion with microsomia, midface hypoplasia, kidney dysplasia, and mild motor retardation was rare. The previous literature showed two novel point mutations in AMMECR1 and CHRDL1 with some phenotype difference from the presented case. Xq23 microdeletion should be considered for patients with microsomia, midface hypoplasia, kidney dysplasia and growth retardation.