电针治疗周围性面瘫疗效观察

目的:寻找能提高治疗周围性面瘫疗效的适宜的电针波型.方法:将147例周围性面瘫患者随机分为治疗组和对照组,治疗组进行穴位分组,电针波型先用连续波后用疏密波治疗;对照组穴位不分组,并单纯采用连续波治疗.结果:两组的总有效率和痊愈所用时间经统计学处理,差异有非常显著性意义(P＜0.01).结论:在电针治疗周围性面瘫中,将疏密波和连续波配合使用,效果显著优于单纯使用连续波者.     

一种适用于聚合酶链反应的常见念珠菌基因组DNA提取方法的研究

目的介绍一种简单、快速的真菌DNA提取方法,提高真菌DNA提取效率,减少毒性和污染性,以适应临床研究需要。方法同时用溶细胞酶结合Biospin真菌基因组DNA提取试剂盒和十六烷基三甲基溴化铵(CTAB)法,提取白色念珠菌、热带念珠菌、克柔念珠菌和净平滑念珠菌基因组DNA,用A260/A280的比值检测DNA的纯度并计算质量浓度,同时行聚合酶链反应(PCR)以评价其可靠性。结果溶细胞酶结合Biospin真菌基因组DNA提取试剂成功提取所用真菌基因组DNA,其纯度及质量浓度能满足PCR反应的要求。结论用溶细胞酶结合Biospin真菌基因组DNA提取试剂提取DNA,简单、快速、高效,可用于PCR反应。     

手足口病的预防控制及护理对策

从手足口病概述,流行概况,预防措施、护理对策及健康教育等方面综述了近年来手足口病的流行及护理进展,并阐述了手足口病的预防及健康教育措施.     

五白散胶囊治疗慢性消化道溃疡出血15例观察

目的:观察五白散胶囊治疗慢性消化道出血的疗效.方法:治疗组15例用五白散胶囊治疗,对照组15例用维霉素、果胶铋、止血敏治疗.结果:治疗组总有效率100%,对照组总有效率86.6%,两组比较有显著性差异(P<0.05).结论:五白散胶囊治疗消化道溃疡出血止血功效良好.     

PRO 1000 V3型心电监护仪LCD逆变器的升级及注意事项

介绍了该型心电监护仪黑屏故障的常见原因，对升级前后的LCD逆变器电路作了对比分析，并详细介绍了升级更换的方法和注意事项。     

Histamine and tryptase modulate asthmatic airway smooth muscle GM-CSF and RANTES release.

Degranulating mast cells are increased in the airway smooth muscle (ASM) of asthmatics, where they may influence ASM function. The aim of the present study was to determine whether histamine and tryptase modulate ASM cell granulocyte-macrophage colony-stimulating factor (GM-CSF) and RANTES (regulated on activation, normal T-cell expressed and secreted) release and also to examine which receptors are involved in this release. Confluent, quiescent ASM cells from asthmatic and nonasthmatic donors were treated with histamine (1 microM-100 microM) with and without histamine receptor antagonist pre-treatment, or the protease-activated receptor (PAR)-2 agonists tryptase (0.5-5 nM) and SLIGKV (100 and 400 microM). The cells were then stimulated with interleukin (IL)-1beta and/or tumour necrosis factor (TNF)-alpha (10 ng.mL(-1)) or left unstimulated for 24 h. Release of GM-CSF and RANTES was determined by ELISA and prostaglandin (PG)E(2) measured by enzyme immunoassay. Neither histamine nor tryptase induced ASM GM-CSF or RANTES secretion. However, histamine increased IL-1beta-induced GM-CSF release and markedly reduced TNF-alpha-induced RANTES release by both asthmatic and nonasthmatic cells to a similar extent, but did not modulate PGE(2) release. All changes involved activation of the histamine H1 receptor as they were partially or fully blocked by chlorpheniramine, but not ranitidine. Tryptase, via its proteolytic activity, also potentiated GM-CSF, but not RANTES, release from asthmatic and nonasthmatic ASM cells induced by both cytokines. PAR-2 involvement in the tryptase potentiation was unlikely because SLIGKV had no effect. In conclusion, mast cells, through histamine and tryptase, may locally modulate airway smooth muscle-induced inflammation in asthma.     

案例教学法在护理伦理学中的应用分析

目的分析案例式教学在护理伦理学教学中应用的效果，以探讨适合护理伦理学的教学方法。方法采用传统教学法与案例教学法相对照进行护理伦理学的授课，对528名授课学生进行问卷调查并收集资料。结果92．4％的学生认为案例教学法能激发学习兴趣，87．2％的学生认为能调动学习的积极性，82．6％的学生认为有利于对知识的理解，72．1％的学生认为能培养思维和决策能力，68．0％的学生认为能增进师生互动。结论案例教学法可提高教学效果。培养学生的批判性思维能力。