Comparison of the treadmill exercise score and single-photon emission computed tomographic thallium imaging in risk assessment

Background  

This study compared the prognostic value of exercise single-photon emission computed tomographic (SPECT) thallium imaging with that of treadmill exercise score in medically treated patients with coronary artery disease (CAD)     

Non-Hodgkin's lymphoma: a differential diagnosis of otogenic facial paralysis

Of all the neoplastic conditions of the lymphatic system, Non-Hodgkin's lymphoma (NHL) represents a heterogenous group. As well as lymph nodes NHL can involve extranodal sites, including regions in head and neck. The mouth and oropharynx are typical extranodal sites, and the ENT surgeon should be aware of this possibility of the swift diagnosis of NHL is to be made. We report two patients with rare invasion of the middle ear, facial nerve paresis, and asymptomatic cerebral involvement by Non-Hodgkin's lymphoma.     

中重型β地中海贫血基因类型分析

目的 了解中间型和重型β地中海贫血基因突变类型及其相关血液，生化变化。方法 应用反向点杂交法检测16种β地贫点突变基因，常规进行血细胞分析及其他地贫筛查试验。结果 27例中重型β地贫患者中10例为纯合子，17例为双重杂合子，10例纯合子中5例CD41-42纯合子，占50%，3例TATAnt-28纯合子，占30%；CD17纯合子和IVS-nt654纯合子各1例，各占10%，17例双重杂合子中5例为IVS-nt654/CD41-42占29.4%,3例为CD17/CD41-42，占17.6%;3例为TATAnt-28/CD41-42，占17.6%;2例为βE/IVS-nt654,占11.6%;CD17/TATAnt-28,IVS-nt654/CD17,IVS-nt654/CD1-1,1CD71-72/IVS-nt654各1例，各占5.9%。27例患者血红蛋白均低于90.0g/L。平均为58.8g/L；平均MCV为65.0fl。脆性均少于60%。结论 中重型β地中海贫血基因突变类型多样，组合类型不同，临床表现轻重不一，多呈中重度贫血，血液，生化均有明显改变，多需输血维持生命，故应做好产前诊断，预防其出生。     

利用cDNA微阵列技术筛选成年期与胚胎期睾丸差异表达基因——钙联接蛋白基因

目的 :用cDNA微阵列技术克隆和筛选成年期与胚胎期睾丸差异表达基因 ,以进行睾丸发育及精子发生的调控研究。　方法 :构建人睾丸cDNA芯片 ,分别用正常成人及胚胎睾丸的mRNA探针进行杂交 ,对成人和胚胎睾丸中差异表达的基因进行高流量的比较。　结果 :发现 1条成人睾丸高表达、胚胎低表达的基因———钙联接蛋白 (CLGN)基因。　结论 :运用cDNA微阵列方法可筛选到成年期与胚胎期睾丸差异表达基因     

Differentiation plasticity of human fetal articular chondrocytes.

OBJECTIVE: To test chondrogenic differentiation potential, we examined the differentiation plasticity of isolated human fetal articular chondrocytes (HFACs). STUDY AND DESIGN SETTING: Culture-expanded human fetal articular chondrocytes (HFACs) were analyzed for chondrogenic, adipogenic, osteogenic capacity and neural differentiation ability in defined in vitro culture systems. RESULTS: The different assays demonstrated that culture-expanded HFACs have potential to form cartilage in pellet mass culture, to form adipose cells, osteogenic cells, and neural cells in monolayer culture. CONCLUSIONS: These results suggest that within human fetal articular cartilages there are MSC-like cells that exhibit differentiation plasticity that is comparable with that of BM-MSCs and they may be a new kind of seeding cells for head and neck cartilage reconstruction.     

使用自制Ralph刀切光镜用的高质量半薄切片及染色

在1981年,我们设计了BD—8102组织切片刀制刀机。该机能制出宽为25mm及38mm的高质量玻璃刀(Ralph)。借助一个简单刀架把Ralph刀装配到普通切片机上,就可切出用塑料或石蜡包埋的组织大切片。用这种方法可得到光镜用的高质量半薄切片。并报告近几年来用该刀切片和染片技术的体会。     

Preliminary study on the nucleic acid hybridization technic in detecting HSV-2 DNA corresponding sequence of cancer tissues of the uterine cervix

In this paper, 34 samples, 25 from 18 cervical carcinoma patients and 9 from 8 non-cervical carcinoma patients, were assayed using (alpha-32P) dCTP HSV-2 DNA probe and nucleic acid hybridization technic. It was shown that the positive rates of HSV-2 DNA corresponding sequence were 50% (2/4), 36% (5/14) and 12% (1/8) in the in situ cancer, invasive cancer and non-cervical cancer tissues. No HSV was isolated from 7 cancer specimens which showed a positive reaction by nucleic acid hybridization.     

门静脉高压症胃病与幽门螺杆菌感染关系的临床研究

目的 :探讨门静脉高压症胃病与幽门螺杆菌感染的关系。方法 :对 68例门静脉高压症胃病患者的上消化道粘膜病理改变、出血状况及与幽门螺杆菌感染之间的关系进行分析。结果 :肝功能受损越重 ,门静脉高压症胃病患者的病理改变越明显。在重度门静脉高压症胃病患者中 ,幽门螺杆菌的检出率为 69.44% ,而在轻度患者中 ,幽门螺杆菌的检出率为 43.75% ,两者比较有显著性差异 (χ2 =4.57,P<0 .0 5)。结论 :门静脉高压症胃病与幽门螺杆菌感染有一定的关系 ,病症越重 ,幽门螺杆菌的检出率越高