缺锌对大鼠力竭性游泳前后睾酮和锌水平变化的影响

本实验动态观察了缺锌和缺锌后补锌对大鼠力竭性游泳前后辜丸和血清睾酮、锌水平以及游泳能力的影响。结果显示：缺锌造成大鼠游泳能力大大降低，补锌后明显增加。不论安静时还是力竭性游泳后１２小时内，缺锌大鼠的血清和辜丸锌含量均显著低于对照和补锌大鼠。与对照和补锌大鼠相比，缺锌大鼠的血清和睾丸酮含量，安静时均较低，力竭性游泳后也呈现不同程度的降低。缺锌还造成大鼠辜丸重量的明显下降。结果表明，锌营养不良将损害运动能力。     

Systemic necrotizing vasculitis associated with childhood sarcoidosis

Childhood sarcoidosis is a rare disorder with protean manifestations. The case of a child with prolonged fever, hepatosplenomegaly, pancytopenia, and systemic necrotizing vasculitis manifesting as fever, rash and skin infarctions, digital pregangrene, and foot drop is reported. This is the first case of systemic necrotizing vasculitis reported in sarcoidosis. The fulminant course of the disease required treatment with intravenous pulsed cyclophosphamide and high doses of corticosteroids. The spectrum of vasculitis in childhood and adult sarcoidosis is reviewed.     

Properties of circulating IgA molecules in Henoch-Schonlein purpura nephritis with focus on neutrophil cytoplasmic antigen IgA binding (IgA-ANCA): new insight into a debated issue

Background: The presence and the pathogenetic role of circulating IgA reacting with neutrophil cytoplasmic antigens (IgA-ANCA) in patients with Henoch-Schonlein purpura (HSP) is still debated. This study was aimed to investigate some characteristics of serum IgA and macromolecular IgA in HSP patients, focusing on IgA-ANCA. Methods: Eighty-seven HSP patients with biopsy proved renal involvement (51 adults and 36 children) enrolled in a multicentre study of the Italian Group of Immunopathology were investigated. Results: Significantly high levels of IgA immune complexes were found in both adults (P <0.05) and children (P <0.01), while the binding of IgA to jacalin, was significantly low in children with HSP (P <0.01) only. Two series of ELISA were done for IgA-ANCA, in two different laboratories. Increased binding to PMN crude extracts (P <0.01) without any modification in IgA binding to proteinase 3 was found by either specific ELISA. Conversely, the binding of IgA to myeloperoxidase (MPO) was found to be significantly (P <0.05) increased with positive values in 25% of patients by one assay only. Three of four sera with positive IgA-MPO ANCA exhibited binding in Western-blot studies with the MPO preparation used in ELISA to a 28-kDa species. D-galactose and N-acetyl-glucosamine decreased the binding of serum IgA to MPO more in HSP than in controls (P <0.05). Conclusions: The conflicting reports on IgA-ANCA may reflect some atypical characteristics of the reaction which can be detected only by some ELISAs. We suggest that not an antigen-antibody reaction but a lectinic interaction due to abnormal composition of IgA carbohydrate side chains may account for the IgA-ANCA reaction in patients with HSP nephritis.     

锰超氧化物岐化酶模型配合物的研究

为探索天然超氧化物岐化酶（ＳＯＤ）岐化超氧离子的机理，及用于临床的可能性，我们合成了乳酸、柠檬酸和酒石酸的锰（Ⅱ）配合物作为Ｍｎ－ＳＯＤ的模拟物，用光照法测定了它们的活性，得到了有意义的结果：锰配合物作为Ｍｎ－ＳＯＤ的模拟物，有肯定的活性，其中以乳酸锰配合物活性最高。     

均一吸收体中SPECT重建多解性的探讨

通过对重建图像噪声特性的分析，间接证明ＳＰＥＣＴ的解具有多样性。分别用ＴｒｅｔｉａｋＭｅｔｚ、Ｇｕｌｂｅｒｇ、富谷武浩等推导的３种等价重建方法和Ｂｅｌｉｎｉ等推导的重建方法，经计算机模拟重建衰减均匀分布的图像，先预置相同的重建图像的空间分辨率，再比较它们的噪声特性。两种方法重建图像的噪声分布：前者近图像边缘时增大，而后者减小，从而间接证明在假定均匀衰减条件下，ＳＰＥＣＴ的重建有多解性。     

Organic anion transport in HepG2 cells: absence of the high-affinity, chloride-dependent transporter.

In previous studies, we identified a 55 kD organic anion-binding protein in liver cell sinusoidal plasma membrane subfractions. Other investigators identified another 55 kD bromosulfophthalein/bilirubin binding protein on the surface of rat hepatocytes and HepG2 cells and suggested that this protein served as a transporter for these ligands. In this study, transport of 35S-sulfobromophthalein by the human hepatoma cell line, HepG2, was quantified in the presence and absence of bovine serum albumin to further clarify the possible function of these plasma membrane binding proteins. In contrast to results in normal rat hepatocytes, virtually no uptake of 35S-sulfobromophthalein by HepG2 cells in the presence of bovine serum albumin was found. In the absence of albumin, HepG2 cells expressed temperature-dependent uptake of 35S-sulfobromophthalein. However, the high-affinity Cl(-)-dependent sulfobromophthalein transport that characterizes normal rat hepatocytes was absent, as indicated by an approximately 95-fold lower affinity and 170-fold higher capacity of HepG2 cells for sulfobromophthalein compared with previous results with rat hepatocytes. These results suggest that 55 kD sulfobromophthalein/bilirubin-binding protein on the liver cell surface differs from organic anion-binding protein and is not responsible for sulfobromophthalein extraction in the presence of albumin, although it may play some role in lower affinity transport by cells. Immunoblot analysis and metabolic labeling of HepG2 cells demonstrated synthesis of organic anion-binding protein. However, light microscopic immunocytochemistry and immunoprecipitation of surface iodinated rat hepatocytes and HepG2 cells with antibody to a recombinant organic anion-binding protein fusion protein indicated absence of organic anion-binding protein on the surface of HepG2 cells.(ABSTRACT TRUNCATED AT 250 WORDS)     

国人脑内某些解剖结构定位研究与临床应用

本文通过对４５个正常成人脑标本进行三维连续２ｍｍ切片，对Ｆｏｒｅｌ－Ｈ，隔区等九个脑内重要结构的解剖坐标及毗邻关系进行观测，为立体定向手术提供解剖学依据。５５例临床实践证明手术疗效比较满意。     

吗啡受体显像剂7α-o-IA-DPN的合成

设计、合成了一种新的吗啡受体显像剂。选用吗啡拮抗剂特培洛啡（ｄｉｐｒｅｎｏｒｐｈｉｎｅ，ＤＰＮ），经乙酰化选择性保护苯环３位—ＯＨ，将乙烯锡烷作为弥补基引入ＤＰＮ分子７α支链淑醇基团位置，采用碘代锡烷标记法一步合成碘烷ＤＰＮ（７αｏｉｏｄｏａｌｙｌｄｉｐｒｅｎｏｒｐｈｉｎｅ，即７αｏＩＡＤＰＮ）。其标记率达９０％，比活度为８０ＴＢｑ／ｍｍｏｌ，放化纯度大于９５％。体外吗啡受体结合分析表明其具有很高的亲和力（Ｋｉ＝０．４ｎｍｏｌ／Ｌ）。结果表明：新合成的碘标记吗啡配体适合于ＳＰＥＣＴ活体吗啡受体显像研究。