乌鲁木齐地区线粒体12SrRNA基因突变筛查分析

目的　研究乌鲁木齐地区非综合征性聋患者线粒体12S rRNA基因突变情况。方法　收集乌鲁木齐非综合征性聋患者标本609例,对其进行临床和分子遗传学评估。结果　12S rRNA基因突变分析共发现11个突变位点,已知的A1555G、961DelT、C1494T突变分别占2.96%,1.15%,0.16%。另外A1047G突变相关报道较少,A1585G突变未见相关报道。其他突变均为多态性位点。结论　线粒体12S rRNA突变是引起遗传性聋的重要因素,此次乌鲁木齐地区线粒体12S rRNA A1555G突变在聋病人群中的检出率与前期报道相比有所降低,可能与耳毒性药物使用量降低有关。A1047G与新发现的A1585G突变是否与聋相关,还需进一步研究。对筛查中阳性突变携带者及其母系家庭成员需告知氨基糖苷类抗生素使用风险,使其避免使用,逐步降低药物性聋的发生率。     

Effect of low-dose lidocaine on MEPs in patients undergoing intracranial tumor resection with propofol anesthesia: A randomized controlled trial

Objective:To investigate the effect of low-dose lidocaine on motor evoked potentials (MEPs) in patients undergoing intracranial tumor resection with propofol anesthesia.Methods:Forty patients who underwent intracranial tumor resection and required MEP monitoring were selected. They were randomly divided into the lidocaine group (group L, n = 20) and the control group (group C, n = 20) by computer-generated randomization. All patients were given propofol anesthesia under the guidance of the bispectral index. In group L, 1 mg/kg of lidocaine was injected intravenously during anesthesia induction. Then, lidocaine was continuously pumped at a speed of 1 mg/kg/h until the operation started. Group C was given an equal volume of normal saline. Heart rate (HR), mean artery pressure (MAP), and bispectral index were recorded before anesthesia induction (T0), 2 minutes after tracheal intubation (T1), and 35 minutes (T2), and 50 minutes (T3) after anesthesia induction. The amplitude and latency of MEP at T2 and T3, the total dosage of propofol after anesthesia induction, and adverse events before T3 were recorded.Results:Compared with those in group C, HR and MAP were significantly decreased at T1 in group L. No significant differences were observed in HR and MAP at T0, T2, and T3 between group L and group C. The total dosage of propofol and the incidence of adverse events were significantly lower in group L than in group C before T3. There were no significant differences in the amplitude and latency of MEP between the 2 groups at each time point.Conclusions:Low-dose lidocaine has no obvious effect on MEP in patients undergoing intracranial tumor resection. However, it increased hemodynamic stability, reduced propofol use, and decreased the incidence of adverse events.     

基于知识元标引的中医古籍研究回顾与反思

回顾国内以往知识元研究,结合当前中医古籍知识元研究工作,探讨中医古籍研究的部分现存问题.中医古籍知识元研究工作重点在于知识元标引平台的研发以及中医基础理论的数据化处理.知识元的概念是文本中的词或短语;知识元的文本可以借助文言语法与知识图谱三元组概念进行数据提取;建立语义关系,即语义三元组的描述,可分为表达不同描述对象(...     

吡喹酮长循环纳米粒在家兔体内的药动学

健康成年家兔18只,随机分为3组,分别静注吡喹酮溶液、吡喹酮纳米粒和吡喹酮长循环纳米粒(10 mg/kg).以地西泮为内标,采用HPLC法测定家兔血浆中的吡喹酮.吡喹酮注射液和吡喹酮纳米粒的体内过程符合二房室模型,吡喹酮长循环纳米粒的体内过程符合三房室模型,3种制剂的主要药动学参数分别为:t1/2(1.27±1.56)、(1.13±0.66)和(23.82±8.94)h,CL(5.29±0.78)、(2.38±1.00)和(1.16±0.16)L·h-1·kg-1,AUC0→t(1.48±0.2 7)、(3.52±1.79)和(4.93±1.27) mg·h·ml-1,MRT(0.44±0.30)、(0.41±0.10)和(16.12±1.38) h.     

Effects of population aging on the mortality burden of related cancers in urban and rural areas of China, 2004–2017: a population-based study

Objective: China is a developing country with urban–rural disparities and accelerating population aging. Therefore, quantifying the effects of population aging on the cancer mortality burden is urgently needed.Methods: Using data from China’s death surveillance datasets(2004–2017), we decomposed and quantified the effects of population aging and factor variations on cancer mortality rates in urban and rural China during 2004–2017 through a decomposition method. R ratios were used to assess the e...     

BERH-2肝癌大鼠红细胞膜K~+依赖性磷酸酶的活性

BERH-2肝癌大鼠红细胞膜K~+依赖性磷酸酶活性与正常大鼠无显著差异。但是,K~+对酶的变构激活及F~-对酶的变构抑制却差别显著,其Hill系数不同于正常大鼠。此差别在0.10mg/ml Triton X-100存在时消失,因此,Hill系数的差异可能由于BERH-2肝癌大鼠红细胞膜中脂质成分与正常的不同所致。     

Healon~([R])和Viscoat~([R])细胞毒性的比较研究

本实验应用新的组织培养系统培养的兔角膜内皮细胞,对Healon~(R)和Viscoat~(R)的细胞毒性进行了比较研究。发现它们对角膜内皮细胞具有潜在的毒性作用,当细胞暴露于药液3小时后,出现轻度的损害。24小时后,出现严重的细胞损害。两者相比较,Viscoat~(R)对细胞的损害程度比Healon~(R)的轻,提示Viscoat~(R)可替代Healon~(R)用于眼科手术。     

颅内血肿体积个人计算机测量方法

目的:研究个人计算机测量法对颅内血肿体积测量的价值.方法:40例不同类型的颅内血肿分别使用CT定量软件及个人计算机Photoshop CS3 Extented软件测量其体积,分析不同方法的测量结果是否具有统计学意义.结果:个人计算机测量法与CT定量法所测颅内血肿体积差异无统计学意义(P＞0.05).结论:个人计算机测量...     

后路椎间融合器结合新型椎弓根钉系统治疗腰椎滑脱症

目的 探讨纯钛多孔涂层后路椎间融合器结合新型椎弓根钉系统对腰椎滑脱症的治疗效果。方法 33例腰椎滑脱症病人,采用后路全椎板切除减压、纯钛多孔涂层后路椎间融合器行椎间融合、新型椎弓根钉系统内固定治疗。结果 33例术后均随访,时间6~36个月,平均18.6个月。本组患者术后无神经损伤和切口感染,动态摄片观察无融合器移位、椎弓根螺钉松脱、断裂等并发症。按Brantigan评价标准评定结果:优21例、良10例、可2例、差0例。结论 纯钛多孔涂层后路椎间融合器结合新型椎弓根钉系统治疗腰椎滑脱症,具有椎管减压充分、椎体复位好、术后能立即改善疼痛症状、立即稳定椎体、病人可早期下床等优点,是一种治疗腰椎滑脱症较理想的方法。