Electrophysiological study on differentiation of rat bone marrow stromal stem cells into neuron-like cells in vitro by edaravone

To explore the electrophysiological proper-ties of differentiation of rat bone marrow-derived stromal stem cells (rBMSCs) to neuron-like cells in vitro by edaravone, a new type of free radical scavenger. Methods: Stromal stem cells were separated from rat bone marrow with Ficoll-Paque reagent and expanded in different culture medium in vitro, rBMSCs were induced by edaravone containing serum-free L-DMEM. Morphologic observation and Western blot analysis including the ex-pression of Nav1.6, Kv1.2, Kv1.3, Cav1.2 were performed, and whole patch-clamp technique was used. Results: Cyton contraction and long processes were shown in differentiated stromal stem cells. Nav1.6, Kv1.2, Kv1.3 and Cav1.2 were expressed in both differentiated and undifferentiated cells. However, the expression of channel proteins in differentiated cells was up-regulated. Consistently, their resting potential and outward currents were also enhanced in the differentiated cells, which was especially significant in the outward rectifier potassium current. Conclusion: In vitro, neuron-like cells derived from rBMSCs, induced by edaravone, possess electrophysiologi-cal properties of neurons.     

89例小脑幕脑膜瘤的显微外科手术治疗

目的 总结小脑幕脑膜瘤的手术治疗经验。方法 回顾分析1993年5月-2006年5月，89例经手术治疗的小脑幕脑膜瘤病人的临床表现、影像学检查、手术记录、病理及术后早期并发症。常见症状是头痛（54例，60．7％）、头晕（29例，32．6％）、步态异常（34例，38．2％）、听力损害（27例，30．3％）及面部疼痛（20例，22．5％）。主要体征是共济失调（38例，42．7％）、运动和感觉障碍（31例，34．8％）及脑神经损害（48例，53．9％）。多数病例MRI表现为等T1、T2信号并明显均一强化。结果 本组全切除65例（73．0％），次全切除23例（Okudera-KobayashiⅣ—Ⅴ级）。手术并发症的发生率和病死率分别为22．5％和1．1％。结论 根据小脑幕脑膜瘤部位以及与周围神经血管关系选择最佳手术入路。如全切肿瘤可能带来重要的神经功能损害，应考虑残留部分肿瘤。     

可吸收螺钉治疗踝关节骨折的临床应用

[目的]探讨可吸收螺钉（PGA-PLLA）在治疗踝关节骨折中的应用。[方法]1994年3月-2003年8月治疗踝关节骨折42例，皆行切开复位可吸收螺钉内固定术。下胫腓联合分离仍不稳定者，用可吸收螺钉横向固定。[结果]全病例得到6个月-5年3个月随访，平均2年4个月。根据美国足踝外科协会（AOFAS）制定的踝关节评定标准，优32例，良8例，可1例，差1例。优良率达95．2％。[结论]可吸收螺钉治疗踝关节骨折，具有良好的内固定特性与组织相容性，吸收完全，能早期进行功能锻炼，避免了再次手术的痛苦。     

用人工合成材料的吊带经耻骨固定或经腹壁固定治疗真性女性压力性尿失禁

目的总结用人工合成材料的吊带经不同的途径治疗女性压力性尿失禁的方法和结果。方法采用人工合成材料的吊带经耻骨固定（In—Fast）技术治疗3例，用经腹壁固定（TVT和IVS）技术治疗13例。结果绝大多数病人均排尿通畅．无尿失禁复发。但TVT组有1例排尿不畅，3个月后剪断吊带后变为轻度尿失禁。In-fast组有1例性交不适伴阴道分泌物增加。结论用人工合成材料的吊带进行悬吊技术治疗女性真性压力性尿失禁是安全、微创和有效的手术方式。经耻骨固定技术和用经腹壁固定技术这两种方法各有自己的优缺点。应根据病人的具体情况去选择使用。     

增白牙膏去除牙面外源性色素临床实验

目的测试一种增白牙膏用于减少牙面外源性色素的效果. 方法 研究对象121例,年龄21～57岁.实验组60例,对照组61例,两组年龄和性别分布差异无显著性.采用双盲法两组平行设计,根据Lobene色斑指数(LSI)和性别均衡分为两组,随机分配使用实验牙膏或对照牙膏.实验组牙膏含3 %植酸钠和1.3 %焦磷酸钠,对照组牙膏不含相应成分.临床检查分别在基线、3周和6周后进行,由同一人测定LSI.问卷询问刷牙情况. 结果 基线检查、实验3周和6周后实验组LSI分别为3.00、2.16和1.47, 对照组分别为3.10、3.08和2.18.基线检查两组LSI差别无显著性意义, 实验3周和6周后两组LSI比较差异均有显著性意义. 实验组LSI在3周和6周后分别比基线时降低28 %和51 %. 结论含3 %植酸钠和1.3 %焦磷酸钠牙膏能有效减少牙面色素,效果随使用时间而增强.     

首次CT检查阴性的颅脑损伤54例临床分析

目的分析颅脑损伤首次CT检查阴性而复查CT阳性的表现和原因。方法通过对首次CT检查阴性而复查CT阳性的54例颅脑损伤患者（研究组）的临床资料进行回顾性分析总结,并与同期收治的325例首次CT及复查CT均为阴性的颅脑损伤患者（对照组）作比较。结果研究组首次CT检查阴性而复查CT阳性表现为迟发性血肿、脑挫裂伤、脑积水、硬膜下积液、弥漫性脑肿胀、外伤性脑梗塞等,可由不同原因引起。结论熟悉本类颅脑损伤首次CT检查阴性,而复查CT阳性的表现及原因,对于指导检查及治疗,改善病人预后有重要意义。     

+10 Gz重复暴露大鼠脑差异表达基因cDNA文库的构建

目的构建＋10Gz重复暴露大鼠脑差异表达基因的消减cDNA文库。方法本实验用SD大鼠，分别提取暴露组与对照组的总RNA，并分离纯化mRNA，应用抑制性消减杂交技术分离＋10GI重复暴露大鼠脑差异表达基因eDNA片段并建立消减eDNA文库；利用PCR对随机挑选的75个白色菌落进行插入片段的验证，对其中70个克隆进行eDNA斑点杂交验证。结果所构建的eDNA文库扩增后包含约400个白色克隆和100个兰色克隆，随机挑选75个白色克隆入质粒载体后共获得70个阳性克隆。结论应用抑制性消减杂交技术成功构建了＋10Gz重复暴露大鼠脑差异表达基因消减eDNA文库，为进一步筛选和克隆脑损伤相关基因奠定了基础。     

阿弗菌素产生菌SIPI-AV-99081的选育

阿弗菌素链霉菌SIPI-AV-99081采用UV和NTG进行诱变处理。选育得到一高产菌株和几个典型的突变株。高产菌株AV-h-360的阿弗菌素B1的生产能力较出发菌株提高了3倍，AV-m-486和AV-m-796为阿弗菌素合成阻断突变株，它们都不能产生天然的阿弗菌素。     

局部注射VEGF对大鼠正畸牙齿移动的影响

目的：观察局部注射重组鼠的血管内皮生长因子(rrVEGF)对大鼠正畸牙齿移动的影响，探讨VEGF在正畸牙齿移动中的作用机制。方法：45只雄性SD大鼠，牵引其上颌第一磨牙近中移动，实验中分别将rrVEGF及生理盐水注射入实验组及对照组大鼠右侧上颌第一磨牙腭侧的骨粘膜下，于实验前3天开始注射，每3天一次。分别在加力l、3、7、14、2l天后记录上颌第一磨牙移动距离。然后将各组动物处死，用HE染色观察牙周组织变化情况并采用免疫组织化学方法对组织中表达的VEGF进行分析。结果：实验组大鼠压力侧破骨细胞教和成骨细胞数在实验全过程中均多于对照组，而且，实验组大鼠牙齿移动距离明显大于对照组。结论：进一步证实VEGF作为旁分泌因子，参与了牙齿移动过程中的牙周组织改建，内源性VEGF和注射VEGF都使牙齿移动量显著增加。因此，VEGF在正畸牙齿移动的机制中具有重要意义。     

46例首诊伴远处转移鼻咽癌的预后分析

Objective To retrospectively analyze the therapeutic results of patients with initially di-agnosed metastatic nasopharyngeal carcinoma (NPC). Methods From January 1995 to December 1998, 46 NPC patients with distant metastases were treated in Fujian provincial cancer hospital. Among these pa-tients, 43 were single site metastasis and 3 were multiple sites metastases;The site of metastasis were 19 pa-tients in the liver, 11 in the bone, 7 in the lung, 1 in the brain, 6 in mediastinal nodes and 6 in axillary lymph nodes. All patients received standard radiotherapy to the primary site and cervical node region with a median dose of 72 Gy. Forty-one patients (89%) received 1-5 cycles chemotherapy (cisplatin and 5-flu-orouracil), and 23 (50%) received palliative irradiation to the metastatic site. Results The median surviv-al time was 20 months. The 1-, 2-, 3-year and 5-year overall survival rates were 66%, 47%, 30% and 19%, respectively. Irradiation to the metastatic sites and KPS were the significant prognostic factors. Pa-tients with palliative irradiation to the metastatic site had longer survival than those without (39 months vs. 13 months, X2=8.63, P=0.012). Patients with good performance status (KPS≥80) had better outcomes thanthose with poor performance status (26 months vs. 12 months, X2= 3.95, P=0.035) . Conclusions Active therapy may prolong the survival of patients with initially diagnosed metastatic NPC, especially for those who have good performance status. Under systematic chemotherapy, radiotherapy to the primary site and supportive care, the palliative irradiation to the metastatic site may also yield a good result.