基于相同载体体系的中药成分自乳化制剂的制备及其体外透皮吸收特性考察

目的:制备同时适合于黄芩素、黄连素及大蒜素3种中药成分的自乳化载体体系,并考察这3种自乳化制剂的体外透皮吸收效果。方法:采用饱和溶解度法、伪三元相图法、正交试验筛选最佳的空白处方组成及基质用量。以Franz扩散池和离体大鼠皮肤进行体外透皮吸收试验,采用HPLC分别测定3种自乳化制剂中黄芩素、黄连素及大蒜素的累积透皮渗透量,并分别与黄芩素散剂、黄连素散剂及大蒜素散剂进行比较。结果:最佳处方为油酸乙酯-聚氧乙烯氢化蓖麻油40-聚乙二醇400载体体系,所形成的自乳化制剂粒径适宜,其微观形态呈较规整的球形。透皮吸收10 h,黄芩素、黄连素及大蒜素3种自乳化制剂的药物透皮速率分别为6.898 6,7.600 4,190.040μg·cm~(-2)·h~(-1),累积透皮渗透量分别为71.38,85.54,1 795.16μg·cm~(-2)。结论:成功制备了不同种类中药成分均能使用的自乳化载体体系,且该自乳化制剂的体外透皮吸收效果良好,可为后续中药复方自乳化制剂的制备及其透皮给药吸收研究提供实验依据。     

中医药防治多器官功能障碍综合征探讨

多器官功能障碍综合征(MODS)是创伤及感染后最严重的并发症。近年来,现代医学对MODS发生机制的认识已有长足进步,相对而言,临床疗效就不那么令人满意了。尽管全力采取积极复苏、器官支持、抗感染、介质拮抗等手段,其病死率仍居高不下。因此,越来越多的有识之士认为,运用中医药防治MODS是今后的一个重要发展方向。1　中医药防治MODS的简要回顾中医药治疗MODS是从治疗单一脏器衰竭起步的。王今达等于1972年用血府逐瘀汤治疗天津首例确诊DIC获成功。1976年根据ARDS多有苔黄燥、脉洪大、腹胀满、大便秘结之阳明腑实证,选凉膈散为主方…     

培育颗粒对人绒毛滋养细胞侵袭与增殖的作用

目的观察培育颗粒对人绒毛滋养细胞的侵袭和增殖功能的影响。方法取人妊娠5~10周正常胎盘绒毛,进行滋养细胞培养。正常23~25 d雌性SD大鼠药物灌胃,取药理血清。培养液加20%血清,分组培养48 h后检测指标。用Transwell侵袭实验检测培育颗粒对滋养细胞侵袭功能的影响,MTT法检测培育颗粒对滋养细胞增殖能力的影响,流式细胞术检测培育颗粒对滋养细胞增殖核抗原(PCNA)表达的影响。结果Transwell侵袭实验检测结果表明,培育颗粒血清各剂量组与正常组侵袭细胞数均有差异,中剂量组侵袭细胞数最多。MTT法检测结果表明,培育颗粒血清各剂量组与正常组吸光度均有差异,中剂量组吸光度最高。流式细胞术检测结果表明,培育颗粒血清各剂量组与正常组PCNA阳性率均有差异,中剂量组PCNA阳性率最高。结论培育颗粒可促进人绒毛滋养细胞侵袭与增殖功能,可能与负反馈调节有关。     

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??Objective To investigate the relationship between CCL2-2518A/G gene polymorphism and severity of enterovirus 71??EV71?? infection. Methods CCL2 gene polymorphism in 188 EV71-infected patients and 235 healthy controls were detected by the improved multiplex ligation detection reaction technique??iMLDR??. The level of CCL2 in two groups was determined by enzyme-linked immunosorbent assay??ELISA??. Results No significant differences were found in the distribution of genotype CCL2-2518A/G between EV71-infected patients and the healthy control group??P??0.05??. The G allele??genotypes AG or GG?? in the CCL2-2518A/G??P??0.001?? was more frequent in patients with severe EV71 infection. The level of CCL2 in infected patients was higher than that of the heathy controls ??P??0.05???? the severe cases had higher level of CCL2 than that of the slight cases and healthy controls??P??0.05??. The level of CCL2 in GG gene group was significantly higher than that in AG gene group and the level of CCL2 in AG gene group was significantly higher than that in AA gene group.The people with CCL2-2518G allele??GG+AG?? had higher level of CCL2 than those only with CCL2-2518A allele??AA????P??0.05??. Conclusion The G carrier of the CCL2-2518A/G is found to be associated with severity of EV71 infection??and could be susceptibility factors in the development of EV71 infection.     

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近年来,随着儿科内分泌遗传代谢病专业不断壮大以及与国外学术交流的增多,国内儿科内分泌遗传代谢病领域的研究进展不断创新。2011年10月13-16日第11届全国儿科内分泌遗传代谢病学术会议在山东烟台隆重举行,来自全国各地的450名正式代表出席了会议,会议收到论文168篇,基本反映了我国儿科内分泌遗传代谢病诊治及研究的整体水平。现就2011年国内儿科内分泌遗传代谢     

Hypertension and diabetes synergistically strengthen the association with cataracts among Chinese

AIM: To examine the relationship between age-related cataracts (ARC) and comorbid hypertension and diabetes. METHODS: We analyzed the administrative records of 6,467 patients aged 50 years and older admitted to the ophthalmological department of a tertiary hospital from January 1st, 2011 to May 20th, 2017. With either eye considered, an ARC (n=3,343) was defined as the presence of lens opacity or previous cataract surgery without evidence of trauma, congenital anomalies or using certain medications. Patients admitted to the same department during the same period due to ocular traumas without clinical evidence of cataracts (n=379) were recruited as the cataract-free controls. Unconditional logistic regressions were obtained the odds ratio (OR) of hypertension and diabetes among ARC patients adjusted for age, sex and health care accessibility. RESULTS: Hypertension was diagnosed in 29.54% of men with any type of cataracts, in 30.12% of men with an ARC, and 10.82% of men of cataract-free controls. Diabetes was diagnosed in 16.64% of men with any type of cataracts, in 16.48% of men with ARC and 4.22% of men of cataract-free controls. Similar patterns were observed among women. After adjusting for age, sex, and health care accessibility, hypertension was weakly [OR=1.83 (95%CI: 1.23, 2.74)] and diabetes was strongly [3.38 (1.86, 6.15)] associated with ARCs. The adjusted OR of comorbid hypertension and diabetes among adults with ARC was 18.20 (4.38, 75.59). CONCLUSION: Hypertension and diabetes were independently associated with ARC. Hypertension and diabetes, if co-existing, multiplicatively strengthened the association with ARC.     

王万春治疗特殊类型毒蛇咬伤经验

王万春认为特殊类型毒蛇咬伤临床上较为少见,包括咬伤特殊人群如孕妇、儿童等,特殊部位如咬伤头面、会阴等.虽情况少见,临床治疗时要考虑多方面因素,但主要为蛇毒对人体的伤害,故采用中西医结合疗法,结合患者情况辨证施治,善用膏药散剂等外敷,内服外敷,中西结合,疗效显著.     

双黄连注射剂不良反应的原因及分析

目的观察、分析和总结双黄连注射剂的不良反应和应对措施。方法通过查阅1994～2008年期间国内公开发行的医药卫生期刊和国家食品药品监督管理局药品不良反应监测中心《信息通报》有关药品不良反应的资料进行分析。结果11083例双黄连注射剂不良反应的病例报告中出现严重不良反应的有306例,涉及158家企业的产品。性别、年龄段没有明显差异;以用药当天出现不良反应为主,严重不良反应的治愈或好转率〉90%,总死亡率高达5.9%,大多数病例对疾病本身影响不大。结论应根据双黄连注射液出现不良反应的可能原因,采取有针对性的安全防治措施。     

高选择性环氧化酶—2抑制剂的进展与评价

目的 :介绍高选择性环氧化酶 - 2抑制剂———昔布类药物进展 ,并评价其临床疗效 ,以供临床参考。方法 :查阅国内、外近期相关文献进行分析、评价。结果与结论 :昔布类高选择性环氧化酶 - 2抑制剂进展十分迅速 ,对环氧化酶 - 2的选择性高 ,在治疗骨关节炎和类风湿性关节炎、关节痛、术后疼痛和肿瘤上展现了良好的治疗前景