神经内镜手术治疗高血压脑出血破入脑室33例

目的探讨神经内镜在高血压脑出血破入脑室病例手术中的应用。方法总结单纯应用神经内镜手术治疗33例高血压脑出血破入脑室的临床资料。结果术后所有病人通过复查头颅CT证实,11例(33.3%)脑室内血肿完全清除,19例(57.6%)脑室内血肿大部分清除,3例(9.1%)脑室内血肿部分清除。33例病人术前格拉斯哥昏迷评分(GCS)8分~14分(10.21分±1.24分),与术后2周(12.34分±0.99分)比较,有统计学意义(t=7.71,P<0.0001)。其中,27例(81.8%)随访2个月,脑室内血肿均完全吸收且脑室系统形态结构正常,无一例发生脑积水,15例临床症状、体征完全消失,12例临床症状、体征较术前明显好转。结论神经内镜治疗高血压脑出血破入脑室,并发症及后遗症相对较少,病人恢复较快,效果确切。     

Humanization and directed evolution of the selenium-containing scFv phage abzyme

According to the binding site structure and the catalytic mechanism of the native glutathione peroxidase (GPX), three glutathione derivatives, GSH-S-DNP butyl ester (hapten Be), GSH-S-DNP hexyl ester (hapten He) and GSH-S-DNP hexamethylene ester (hapten Hme) were synthesized. By a four-round panning with a human synthetic scFv phage library against three haptens, the enrichment of the scFv phage particles with specific binding activity could be determined. Three phage particles were selected binding to each glutathione derivative, respectively. After a two-step chemical mutation to convert the serine residues of the scFv phage particles into selenocysteine residues, GPX activity could be observed and determined upto 3000 U μmol⁻¹ in the selenium-containing scFv phage abzyme which was isolated by affinity capture against the hapten Be. Also the scFv phage abzymes elicited by different antigens displayed different catalytic activities. After a directed evolution by DNA shuffling to improve the affinity to the hapten Be, a secondary library with GPX activity was created in which the catalytic activity of the selenium-containing scFv phage abzyme could be increased 17%. This study might be helpful for new haptens or antigens design to optimize the abzymes with high binding activities and might also provide a novel scheme for GPX mimic candidates for drug development.

A novel two-step sequential screening strategy used for the improvement of GPX mimics and other abzymes.     

Vaginal flora detection in patients receiving controlled ovarian hyperstimulation: nursing care

目的 了解体外受精-胚胎移植(IVF-ET)患者在控制性超促排卵(COH)阶段阴道微生态的变化,提供良好的护理对策.方法 对100例IVF-ET周期中控制性超促排卵患者分别在控制性超促排卵前、后进行阴道微生态检查.结果 IVF-ET周期中的患者在COH前有13.0％、COH后有42.0％发生阴道微生态失调,COH前后比较,差异有统计学意义(P＜0.01).结论 IVF-ET周期中的患者在控制性超促排卵后发生阴道微生态失调的比例较高,要加强相关护理,以减少或避免取卵手术的感染,提高胚胎种植率,降低流产率.     

Engineering the protein targeting two pathways of cerebral ischemia reperfusion injury provides better neuroprotective effect than targeting one pathway

We hypothesized that neuroprotective agents targeting various pathways involved in cerebral ischemia/reperfusion (I/R) injury might be superior to that targeting single pathway. Here, we prepared a fusion protein (B-I) by combining anti-apoptotic Bcl-xL (B) and anti-inflammatory IL-10 (I). B-I could cross blood brain barrier by its N-terminal TAT domain, and be cleaved into separate B and I by Caspase-1. B-I treatment significantly reduced the cerebral infarct volume, better than Bor I treatment alone, and equivalent to B and I treatment (B+I). Treatment with B or B-I significantly attenuated I/R-induced neuronal apoptosis as shown by the decrease in apoptotic rate and the inhibition of caspase-3 activity. Moreover, all recombinant proteins, especially B-I, remarkably attenuated I/R-induced up-regulation of TNF-α. These results suggested that fusion protein B-I inhibiting both inflammation and apoptosis provided better neuroprotective effects than inhibiting either one alone. Our study suggested that multiple pathways targeting brain I-R injury could enhance the neuroprotective effect, and it provided a new idea for the study of neuroprotective drugs for ischemic stroke.     

头颈部血管成像检查中多层螺旋CT双低剂量扫描的应用效果观察

目的 探讨头颈部血管成像检查中多层螺旋CT双低剂量扫描的应用效果。方法 选取2016年1月— 2017年1月在武汉科技大学附属天佑医院接受多层螺旋CT头颈部血管成像检查的患者79例,依据多层螺旋CT管电流与造影剂量差异分组。38例行管电压100?kV、管电流100?mA、对比剂50.0 ml碘克沙醇（320 mgI/ml） 扫描者为对照组。41例行管电压100?kV、管电流80?mA、对比剂40.0?ml碘克沙醇（320?mgI/ml）扫描者为实验组。比较分析两组图像质量主观评分、信噪比、对比噪声比、辐射剂量与碘负荷量。结果 实验组右大脑中动脉、左大脑中动脉、右颈内动脉、左颈内动脉、右颈总动脉、左颈总动脉、主动脉弓信噪比、对比噪声比与对照组比较,差异无统计学意义（P?>0.05）;实验组图像质量评分是（4.34±0.29）分,对照组是（4.32±0.35）分,两组比较,差异无统计学意义（P?>0.05）;实验组容积CT剂量指数、剂量长度积、有效剂量、碘负荷量均较对照组低,差异有统计学意义（P?<0.05）。结论 多层螺旋CT双低剂量扫描应用于头颈部血管成像检查不仅可获取高质量图像,而且可减少辐射剂量,值得选用。     

Higher anxiety rating does not mean poor speech performance: dissociation of the neural mechanisms of anticipation and delivery of public speaking

Public speaking anxiety refers to feelings of nervousness when anticipating or delivering a speech. However, the relationship between anxiety in the anticipation phase and speech delivery phase is unclear. In this study, we used functional near-infrared spectroscopy to record participants’ brain activities when they were anticipating or performing public speaking tasks in an immersive virtual reality environment. Neuroimaging results showed that participants’ subjective ratings of public anxiety in the anticipation phase but not the delivery phase were correlated with activities in the dorsolateral prefrontal cortex, the inferior frontal gyrus, and the precentral and postcentral gyrus. In contrast, their speaking performance could be predicted by activities in the temporal gyrus and the right postcentral gyrus in the delivery phase. This suggests a dissociation in the neural mechanisms between anxiety in preparation and execution of a speech. The conventional anxiety questionnaire is a good predictor of anticipatory anxiety, but cannot predict speaking performance. Using virtual reality to establish a situational test could be a better approach to assess in vivo public speaking performance.

     

我的芭蕾瘦身生涯

芭蕾宁静且高雅．纯洁且含蓄，爱美的你不妨学习一些简单的舞蹈动作，它可以使你身材窈窕、气质高雅、举止迷人……[编者按]     

甘糖酯与脂必妥治疗高脂血症并高粘血症的疗效比较

为观察甘搪酯降低血脂和血粘度的临床疗效，156例住院患者随机分为两组．服甘糖酯者90例为沿疗组．服脂必妥者66例为对照组。服药前分别查血脂4资指标即：胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)，以及血粘6项指标即：全血粘度高切值(ηh)、全血粘度低切值(ηh)、血浆粘度(ηh)、红细胞刚性指数(IR)、红细胞压积(HCT)和纤维蛋白原(Fib)。服药1mo后，分别复查上述各项指标，两组进行对比。结果显示，治疗组TC、TG和LDL3项指标较对照组显著降低，HDL则显著升高（P值均小于0．01)；同时，治疗组6项血粘指标较对照组都显著降低(P值均小于0．01)。观察疗效表明，甘糖酯治疗高脂血症并高血粘症的疗最明显优于脂必妥。