不同浸泡时间对浙贝母有效成分提取的影响

目的以贝母素甲、贝母素乙作为指标成分,研究不同浸泡时间对浙贝母有效成分提取的影响,考察比较合理的煎煮方法。方法采用高效液相色谱一蒸发光散射器（HPLC—ELSD）法检测,DionexAcclaimC18色谱柱（250mm×4．6mm,5μm）,流动相为乙腈-0．1％二乙胺（70：30）,流速为1．0mL／min;ELSD条件为漂移管温度42℃,载气（高纯N2）流速1．0L／min。结果不同浸泡时间对浙贝母饮片有效成分提取量差异明显。随着饮片浸泡时间的延长,煎煮后药渣的白心率降低,而煎膏得率及其有效成分提取量均增高。结论浙贝母饮片用常规煎煮方法很难煮透,至少应先浸泡1h,才能使有效成分较好地溶出,发挥更好的药效。     

Postnatal ontogeny of dopamine D2 receptors in rat striatum

Studies of the ontogeny of dopamine D2 receptors in rat striatum were carried out using [3H]spiroperidol as ligand in the presence of 30 nM ketanserin. D2 receptors increased with age, and saturation studies indicated that this was due to an increase in receptor density (Bmax) and not to a change in affinity (KA). By 21 days of age, receptor density had reached adult levels. Hill plots indicated no cooperativity in the binding from 7 to 21 days of age. Pharmacologically, receptors at 7, 14 and 21 days of age were similar to those of adult rats, exhibiting characteristics of dopamine D2 receptors. These studies provide a basic analysis of dopamine D2 receptors in rat striatum during early postnatal development.     

淋巴滤泡中补体成分沉积的意义

目的:观察补体成分在淋巴组织中的表达,探讨不同发育阶段淋巴滤泡中补体激活与免疫球蛋白沉积及滤泡树突状细胞之间的关系。方法:采用免疫组化方法对淋巴组织标本进行IgG、IgM、IgA、C3c、C4c、C1q和C4d标记,同时对其部分标本进行双标染色。结果:淋巴组织次级滤泡的Ⅰ~Ⅳ期的生发中心才出现不同程度的IgG、IgM、IgA、C3c、C4c、C1q、C4d阳性表达;免疫球蛋白与补体成分重叠沉积,并呈不规则网线状包绕在滤泡树突状细胞周围。其中以C4d的阳性表达最强。结论:淋巴滤泡生发中心有大量免疫球蛋白和补体成分围绕滤泡树突状细胞沉积,可能与中心淋巴细胞的发育和选择有关。     

Quantitative chemical sensing of drugs in scattering media with Bessel beam Raman spectroscopy

Scattering can seriously affect the highly sensitive detection and quantitative analysis of chemical substances in scattering media and becomes a significant challenge for in vivo application of Raman spectroscopy. In this study, we demonstrated a proof of concept for using the self-reconstructing Bessel beam for Raman spectroscopic sensing of the chemicals in the handmade scattering media and biological tissue slices. The homebuilt Bessel beam Raman spectroscopy (BRS) was capable of accurately detecting the Raman spectra of the chemicals buried in the scattering media, and had a superiority in quantitative analysis. The feasibility of the developed technique was verified by detecting the Raman spectra of pure samples in air. Compared with the spectra acquired by the Gaussian beam Raman spectroscope, the performance of the BRS system in terms of Raman spectrum detection and Raman peak recognition was confirmed. Subsequently, by employing the technique for the detection of acetaminophen buried in the scattering media, the application of the new technology in detecting and quantitating the chemicals in the scattering media were underlined, offering greater detection depth and better linear quantification capability than the conventional Gaussian beam Raman spectroscopy. Finally, we explored the potential of the BRS system for chemical sensing of acetaminophen in biological tissue slices, indicating a significant development towards the evaluation of drug in vivo.     

30 d-6°头低位卧床对人下肢脂肪体积的影响

目的 观察模拟失重对人下肢脂肪体积的影响,验证磁共振水脂分离成像技术在测量人下肢脂肪容积中的可行性.方法 14名男性健康志愿者,-6°头低位卧床30 d,使用磁共振水脂分离成像(Dixon)技术采集卧床前后的下肢横断面解剖图像,测量卧床前后下肢脂肪体积.结果 与卧床前相比,卧床后所有志愿者下肢总脂肪体积及大腿段脂肪体积显著增加(P＜0.001);小腿段脂肪体积呈增加趋势,但无统计学差异(P＞0.05).结论 使用MRI水脂分离成像可以清晰地显示卧床前后下肢脂肪体积的变化;-6°头低位卧床30 d后下肢脂肪体积明显增加.     

热乙醇法提取猪血白蛋白工艺改良的探讨

目的介绍运用热乙醇法提取高纯度猪血白蛋白的改良工艺流程。方法采用热乙醇法,在合适的恒温时间和pH值的条件下,分离提纯猪血清白蛋白。结果猪血白蛋白的纯度〉97％,回收率〉80％。检测结果显示,其理化指标均符合《中国生物制品规程2000年版》的要求,适合扩大到中试生产。结论该法与低温乙醇法、利凡诺法和盐析法相比,具有工艺简单、成本低、纯度和回收率高的特点。     

The FAPα-activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway

Osteosarcoma is a kind of bone tumor with highly proliferative and invasive properties, a high incidence of pulmonary metastasis and a poor prognosis. Chemotherapy is the mainstay of treatment for osteosarcoma. Currently, there are no molecular targeted drugs approved for osteosarcoma treatment, particularly effective drugs for osteosarcoma with pulmonary metastases. It has been reported that fibroblast activation protein alpha (FAPα) is upregulated in osteosarcoma and critically associated with osteosarcoma progression and metastasis, demonstrating that FAPα-targeted agents might be a promising therapeutic strategy for osteosarcoma. In the present study, we reported that the FAPα-activated vinblastine prodrug Z-GP-DAVLBH exhibited potent antitumor activities against FAPα-positive osteosarcoma cells in vitro and in vivo. Z-GP-DAVLBH inhibited the growth and induced the apoptosis of osteosarcoma cells. Importantly, it also decreased the migration and invasion capacities and reversed epithelial–mesenchymal transition (EMT) of osteosarcoma cells in vitro and suppressed pulmonary metastasis of osteosarcoma xenografts in vivo. Mechanistically, Z-GP-DAVLBH suppressed the AXL/AKT/GSK-3β/β-catenin pathway, leading to inhibition of the growth and metastatic spread of osteosarcoma cells. These findings demonstrate that Z-GP-DAVLBH is a promising agent for the treatment of FAPα-positive osteosarcoma, particularly osteosarcoma with pulmonary metastases.KEY WORDS: Osteosarcoma, Fibroblast activation protein alpha, Growth, Pulmonary metastasis, Vinblastine prodrug, AXL, β-Catenin     

成人型呼吸窘迫综合征的胸部x线表现

本文对10例 ARDS 病人的胸部甲片进行回顾性分析。发现当临床出现呼吸困难时,7例24小时内所照的胸部平片上,肺部均已出现异常 x 线征象。因此认为胸部 x 光片有助于 ARDS 的早期诊断。发现 PaO_2/FiO_2比值的下降或升高与肺部 x 线的恶化或改善平行.部分患者出现肺部感染(2例),纵隔气肿(2例),等并发症。认为系列胸部照片可判断 ARDS 的改善或进展,并能发现胸部并发症的发生。     

Id4对K562细胞增生的影响及其机制的探讨

为了探讨Id4基因的反义核酸对人红白血病K5 62细胞增生的作用 ,用DNA重组技术将人反义Id4基因插入到真核表达载体pXJ41 neo上 ,重组质粒和空载体分别转染K5 62细胞 ,得到 pAId4和 pK5 62 (对照 )克隆细胞株。发现 :pAId4的生长受到抑制 ,倍增时间加长。免疫印迹实验分析相关蛋白的表达 ,其中CDK4和CyclinD1在 pAId4细胞中的表达量均低于对照细胞 pK5 62 ,而RB蛋白在 pAId4细胞中的表达量高于对照细胞pK5 62 ,揭示了反义Id4引起细胞出现生长抑制的可能机制 ,并探讨了Id4基因对人红白血病K5 62细胞增生的具体作用。研究结果对造血干细胞的体外扩增、分化及红白血病的基因治疗均有重要的参考价值。     

Oral microbiota in the oral-genitourinary axis:identifying periodontitis as a potential risk of genitourinary cancers

Periodontitis has been proposed as a novel risk factor of genitourinary cancers:although periodontitis and genitourinary cancers are two totally distinct types ...