H_2O_2预处理对多巴胺损伤PC12细胞的适应性保护作用

目的探讨H2O2预处理对多巴胺(dopamine,DA)损伤PC12细胞的适应性保护作用。方法透射电镜、Hoechst染色和PI染色流式细胞仪(flowcytometry,FCM)观测细胞凋亡,MTT代谢率法观察细胞线粒体氧化磷酸化功能状态,Rh123染色FCM检测细胞线粒体膜电位(△Ψm)。结果50μmol·L-1DA作用PC12细胞24h后,电镜可观察到PC12细胞体积缩小,核染色质浓缩、边集,核碎裂等细胞凋亡征象。Hoechst33258染色显示,DA(50μmol·L-1)作用24h后,经H2O2预处理的PC12细胞的凋亡量较未预处理的明显减少。50、100和200μmol·L-1DA作用24h后,PC12细胞的凋亡率分别为(20.9±1.8)%、(40.5±6.4)%、(88.1±3.9)%,而经H2O2预处理的PC12细胞的凋亡率分别下降至(4.9±2.9)%、(12.0±1.4)%、(61.5±3.4)%(P<0.01)。20、40、80μmol·L-1DA作用细胞24h后,细胞MTT代谢率明显下降,而H2O2预处理抑制20、40、80μmol·L-1DA引起的PC12细胞MTT代谢率的下降(P<0.01)。50μmol·L-1DA作用24h后,PC12细胞的平均Rh123荧光强度由正常对照的(46.87±0.33)下降至(4.39±2.93),而经H2O2预处理的PC12细胞,其平均Rh123荧光强度仅下降到(10.50±0.28),下降程度明显减轻(P<0.01)。结论H2O2预处理对DA损伤PC12细胞具有适应性保护作用。     

紫苏子油-β-环糊精包合物的制备研究

目的:对紫苏子油-β-环糊精包合物的制备工艺及其性能进行研究。方法:采用正交试验研究制备工艺,利用同步热分析仪对产物的性能进行表征,获得了脱包曲线研究产物的稳定性。结果:通过正交试验及其数据分析,确定制备紫苏子油-β-环糊精包合物的最优工艺为A3B2C1D2和A3B2C3D2,紫苏子油与环糊精的比例对包合率、收率的影响较大;TGA曲线和DSC曲线证明了包合物的生成,显示经过包合后热稳定性增强;脱包曲线显示包合物脱包速率降低,稳定性增强。结论:紫苏子油-β-环糊精包合物的差热、热重和脱包性质,以及包合反应的最佳工艺为紫苏子油的微粉化和制剂的研究、开发提供了依据。     

怀牛膝化学成分的研究

目的 对怀牛膝(Achyranthes bidentata B1．)的化学成分进行分离、鉴定。方法 采用硅胶柱色谱等分离手段；利用理化性质与波谱技术对化合物进行鉴定。结果 得到7个化合物，经鉴定为β-谷甾醇(β-sitosterol，1)，豆甾醇(stigmasterol，2)，齐墩果酸(oleanolic acid，3)，胡萝卜苷(daucosteml，4)，竹节参皂苷-1(PJS-1，5)，正丁基-β-D-吡喃果糖苷(n-butyl-β-D-fructopyranoside，6)，β-蜕皮甾酮(ecdystemne，7)。结论 化合物2、3为首次从怀牛膝中分离得到。     

头孢霉AL031真菌次生代谢产物的抗菌活性研究

采用滤纸片法 ,以 11株革兰氏阳性或阴性细菌为指示菌 ,对从头孢霉AL0 31真菌次生代谢产物中分离到的 14个化合物进行了体外抗细菌活性筛选。结果表明 :新化合物 :S ( ) 5 羟基蜂蜜曲菌素、S ( ) 7 羟基蜂蜜曲菌素和已知化合物 :蜂蜜曲菌素、2 ,4 -二羟基 3,6 二甲基苯甲酸、3,6 二羟基 2 ,4 二甲基苯甲酸、琥珀酸具有广谱的抗细菌活性 ,采用杯碟法 ,以三种细菌为指示菌 ,测定了具有抗菌活性的 6个化合物的最低抑菌浓度 (MIC) ,其MIC为 0 0 312 5～ 0 5mg/ml。     

Phacoemulsification in eyes with corneal opacities after deep anterior lamellar keratoplasty

To evaluate the maneuverability and efficacy of phacoemulsification and intraocular lens (IOL) implantation in eyes with corneal opacities after deep anterior lamellar keratoplasty (DALK), twelve eyes of 12 patients with mild to moderate corneal opacities after DALK and coexisting cataracts were analyzed retrospectively. Phacoemulsification and IOL implantation assisted with anterior capsule staining, as well as non-invasive optical fiber illumination, were performed on all eyes. No intraoperative or postoperative complications were noted. Mean corrected distance visual acuity (logMAR) improved from 1.24±0.17 to 0.73±0.22. Post-phaco intraocular pressure was maintained between 13 to 20 mm Hg in all cases throughout the follow-up period. Mean endothelial cell density decreased from 2258.42±205.94 to 1906.25±174.23 cells/mm2. Phacoemulsification and IOL implantation are safe and valid in eyes with mild to moderate corneal opacities after DALK and coexisting cataracts when assisted with anterior capsule staining and non-invasive optical fiber illumination.     

Alpha角对多焦点人工晶状体植入术后视觉质量影响

研究不同Alpha角对多焦点人工晶状体（MIOL）植入术后视觉质量的影响,指导功能性IOL的应用。方法：前瞻性临床研究。选取2016年12至2018年10月就诊于潍坊眼科医院行白内障超声乳化吸除联合MIOL植入术患者56例（74眼）,根据Alpha角大小（角膜上视轴与光轴的距离α）分为3组： A组12眼（0 mm<α≤0.2 mm）,B组38眼（0.2 mm<α≤0.4 mm）,C组24眼（α>0.4 mm）。术后随访3个月,记录裸眼远、中、近视力以及调制传递函数、眩光、光晕等情况。数据采用卡方检验、方差分析进行统计。结果：B组和C组光晕和眩光发生率高于A组,差异有统计学意义（χ2 =38.372,P=0.01）; 3组间术后3个月远、中、近视力比较差异无统计学意义（均P>0.05）;3组间MTF值在0～30 c/d范围差异无统计学意义（P>0.05）;夜间远视、重影、混合焦点、星芒的发生率差异均无统计学意义（均 P>0.05）。结论：当Alpha角大于0.4 mm时,植入MIOL会增加眩光和光晕的发生,所以Alpha角>0.4 需谨慎考虑使用MIOL。     

Wnt通路中散乱蛋白在儿童急性淋巴细胞白血病中的表达及意义

目的 探讨Wnt信号通路中散乱蛋白（DVL）对儿童急性淋巴细胞白血病（ALL）发病及预后的意义。方法 选取33例新发ALL患儿为病例组,根据危险度将ALL患儿分为低危组（n=14）、中危组（n=5）和高危组（n=14）;29例免疫性血小板减少症（ITP）患儿为对照组。采集病例组患儿初发及诱导治疗第33天时,以及对照组骨髓血各2 mL,采用实时荧光定量聚合酶链反应（qRT-PCR）检测骨髓血细胞DVL1、DVL2、DVL3 mRNA表达。结果 病例组初发期DVL1、DVL2、DVL3 mRNA表达水平高于缓解期及对照组（P < 0.05）,与对照组比较,病例组缓解期DVL2 mRNA水平升高（P < 0.05）。DVL2 mRNA表达水平在病例组初发期及缓解期均高于DVL 1、DVL 3 mRNA （P < 0.05）。高、中危组DVL1、DVL2 mRNA表达水平高于低危组（P < 0.05）。DVL2 mRNA表达水平在低、中、高危组均高于DVL1、DVL3 mRNA （P < 0.05）。结论 Wnt通路中DVL,尤其是DVL2的表达变化可能对儿童ALL的发病及预后有一定意义。     

PSTPIP1‐associated myeloid‐related proteinemia inflammatory syndrome: A rare cause of childhood neutropenia associated with systemic inflammation and hyperzincemia

Neutropenia in pediatric patients can be due to a variety of disorders. We describe two patients who underwent extensive evaluation over many years for arthralgias and moderate neutropenia of unclear etiology. Genetic testing identified a pathogenic variant in PSTPIP1 (proline‐serine‐threonine phosphatase‐interacting protein 1) in both patients. Markedly elevated inflammatory markers and zinc levels confirmed the rare diagnosis of PSTPIP1‐associated myeloid‐related proteinemia inflammatory (PAMI) syndrome, tailoring treatment. Neutropenia is common in patients with PAMI syndrome. Unique mutations seen in PAMI syndrome may account for the specific phenotypic features of this disorder.     

三维可视化引导放疗摆位系统的研发及临床应用

目的:开发一种三维可视化技术辅助放疗患者摆位,并对比分析其在乳腺和盆腔放疗中与传统摆位方法的差异。方法:选取2020年6月至2021年4月常州第二人民医院40例放疗患者作为研究对象,其中乳腺、盆腔患者各20例。利用患者定位CT数据进行三维可视化重建,并将三维可视化模型与真实治疗环境融合,通过交互操作使得三维可视化模型位...     

Early changes in corneal densitometry after FS-LASIK combined with accelerated corneal cross-linking for correction of high myopia

AIM: To observe the effects of femtosecond laser-assisted excimer laser in situ keratomileusis combined with accelerated corneal cross-linking (FS-LASIK Xtra) on corneal densitometry after correcting for high myopia. METHODS: In this prospectively study, 130 patients underwent FS-LASIK or FS-LASIK Xtra for high myopia. Their right eyes were selected for inclusion in the study, of which 65 cases of 65 eyes in the FS-LASIK group, 65 patients with 65 eyes in the FS-LASIK Xtra group. Patients were evaluated for corneal densitometry at 1, 3, and 6mo postoperatively using Pentacam Scheimpflug imaging. RESULTS: Preoperative differences in corneal densitometry between the FS-LASIK and FS-LASIK Xtra groups in different ranges were not statistically significant (P>0.05). Layer-by-layer analysis revealed statistically significant differences in the anterior (120 μm), central, and total layer corneal densitometry between the FS-LASIK and FS-LASIK Xtra groups at 1 and 3mo postoperatively (all P<0.05), the FS-LASIK Xtra group is higher than that of the FS-LASIK group. Analysis of different diameter ranges showed statistically significant differences between the FS-LASIK group and the FS-LASIK Xtra group at 1mo postoperatively in the ranges of 0–2, 2–6, and 6–10 mm (both P<0.05); At 3mo postoperatively, the FS-LASIK Xtra group is higher than that of the FS-LASIK group in the ranges of 0–2 and 2–6 mm (P<0.05). At 6mo postoperatively, there were no statistically significant differences in corneal densitometry between the FS-LASIK group and the FS-LASIK Xtra group in different diameter ranges (all P>0.05). CONCLUSION: There is an increase in internal corneal densitometry during the early postoperative period after FS-LASIK Xtra for correction of high myopia. However, the densitometry values decreased to the level of conventional FS-LASIK at 6mo after surgery, with the most significant changes observed in the superficial central zone.