三种腹腔镜卵巢子宫内膜异位症囊肿剥除术中不同止血方法对患者卵巢储备功能的影响

目的探讨三种腹腔镜卵巢子宫内膜异位囊肿剥除术中不同止血方法对患者卵巢储备功能的影响。方法选取本院2011年4月～2012年1月收治的122例卵巢子宫内膜异位囊肿患者作为研究对象．常规行腹腔镜卵巢子宫内膜异位囊肿剥除术,按术中不同止血方法分为超声刀组（31例）、双击电凝组（33例）和缝合组（58例）,分别在入院时及术后1、3、6、12个月检测患者FSH及AFC水平,在术后24个月调查患者的自然妊娠情况。比较不同止血方法对三组患者卵．巢储备功能的影响。结果入院时三组患者FSH水平比较差异无统计学意义（P〉0．05）;术后1、3、6、12个月时三组患者FSH水平较入院时不同程度升高,差异有统计学意义（P〈0．05）,且缝合组低于超声刀组和双击电凝组,差异有统计学意义（P〈0．05）。入院时及术后1个月三组患者AFC水平比较差异无统计学意义（伶0．05）;在术后3、6、12个月时缝合组患者AFC水平较超声电刀组和双击电凝组高,差异有统计学意义（P〈0．05）。术后24个月,缝合组患者的自然妊娠率达36．2％,高于双击电凝组和超声刀组,差异有统计学意义（F=7．285,P〈0．05）。结论在卵巢子宫内膜异位囊肿腹腔镜下剥除术中应用超声刀止血法和双击电凝止血法均会降低患者卵巢储备功能,影响患者自然妊娠能力,而缝合法相对影响较小。     

African Swine Fever Virus Structural Protein p17 Inhibits Cell Proliferation through ER Stress—ROS Mediated Cell Cycle Arrest

African swine fever virus (ASFV) is a highly pathogenic large DNA virus that causes African swine fever (ASF) in domestic pigs and wild boars. The p17 protein, encoded by the D117L gene, is a major transmembrane protein of the capsid and the inner lipid envelope. The aim of this study was to investigate the effects of p17 on cell proliferation and the underlying mechanisms of action. The effects of p17 on cell proliferation, cell cycle, apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress have been examined in 293T, PK15, and PAM cells, respectively. The results showed that p17 reduced cell proliferation by causing cell cycle arrest at G2/M phase. Further, p17-induced oxidative stress and increased the level of intracellular reactive oxygen species (ROS). Decreasing the level of ROS partially reversed the cell cycle arrest and prevented the decrease of cell proliferation induced by p17 protein. In addition, p17-induced ER stress, and alleviating ER stress decreased the production of ROS and prevented the decrease of cell proliferation induced by p17. Taken together, this study suggests that p17 can inhibit cell proliferation through ER stress and ROS-mediated cell cycle arrest, which might implicate the involvement of p17 in ASF pathogenesis.     

Curative efficacy might be an early predictor of prognosis in patients with small cell lung cancer treated with 2 cycles of platinum-based first-line chemotherapy

BackgroundPlatinum-based chemotherapy remains the essential therapy for small cell lung cancer (SCLC). Here, we conducted a statistical analysis to explore whether the curative efficacy of 2-cycle platinum-based chemotherapy can predict the survival of patients with SCLC.MethodsFifty-six SCLC patients who had each received 2 cycles of platinum-based chemotherapy were enrolled. The curative efficacy of the chemotherapy was evaluated, mainly by chest computed tomography, and the treatment response was categorized according to the Response Evaluation Criteria in Solid Tumors (RECIST) 1.1. Patients were continuously followed up for progression-free survival (PFS) and overall survival. The 55 patients were separated into 2 groups by the curative efficacy of the 2-cycle first-line platinum-based chemotherapy. All statistical analyses were performed with SPSS software (version 17.0; SPSS, Inc.; Chicago, IL, USA)ResultsPatients who responded to 2-cycle chemotherapy (partial response, PR) had significantly better survival than others who did not (stable disease, SD or progressive disease, PD). The median progression-free survival (mPFS) in the PR group was 6.330 months, which was significantly longer than the 2.870 months seen in SD+PD group (95% CI: 4.631–8.029 vs. 0.000–5.790, P=0.022). The median overall survival (mOS) was 10.870 months in the PR group, which was remarkably longer than the 8.970 months observed in the SD+PD group (95% CI: 9.546–12.194 vs. 6.517–11.423, P=0.028). Curative efficacy had no correlation with clinical features.ConclusionsThe curative efficacy of 2-cycle first-line platinum-based chemotherapy was significantly correlated with PFS and OS, and showed prognostic value in SCLC patients. Patients who were sensitive to chemotherapy had superior survival to those who were chemotherapy insensitive.     

减重代谢术前患者肥胖相关病耻感的质性研究

目的深入了解减重代谢手术患者术前肥胖相关的病耻感体验,以期为提高患者手术信心和术后自我管理质量提供参考。 方法选择北京市某三级甲等医院9名拟行减重代谢手术的患者为研究对象,采用半结构式访谈法,运用主题分析法对访谈资料进行分析、归纳及提炼主题。 结果减重代谢手术患者的术前病耻感体验归结为4个类属。类属一：病耻感体验,即自卑感、孤独、自责和被歧视的经历;类属二：病耻感来源,一方面来自自身,另一方面来自他人,如亲友和同事、医护人员、公众;类属三：病耻感的应对,包括盲目采取各种方法,减少社会活动、逃避、持有积极减重的态度;类属四：对手术改善肥胖的态度,包括经他人推荐相信减重效果,或对手术了解较少抱着尝试心态。 结论减重代谢手术的患者术前存在病耻感体验,医护人员应为其提供相应的心理支持,指导其采取正确的应对方式;同时应做好社会健康教育,加强公众对肥胖与代谢疾病的认识,正确认识减重代谢手术在治疗肥胖与代谢疾病中的效果。     

Thermally stable La2LiSbO6:Mn4+,Mg2+ far-red emitting phosphors with over 90% internal quantum efficiency for plant growth LEDs

In this paper, we reported on the high-efficiency and thermally-stable La₂LiSbO₆:Mn⁴⁺,Mg²⁺ (LLS:Mn⁴⁺,Mg²⁺) far-red emitting phosphors. Under 338 nm excitation, the composition-optimized LLS:0.3%Mn⁴⁺,1.6%Mg²⁺ phosphors which were made up of [SbO₆], [LiO₆], and [LaO₈] polyhedrons, showed intense far-red emissions peaking at 712 nm (²E_g → ⁴A_2g transition) with internal quantum efficiency as high as 92%. The LLS:0.3%Mn⁴⁺,1.6%Mg²⁺ phosphors also exhibited high thermal stability, and the emission intensity at 423 K only reduced by 42% compared with its initial value at 303 K. The far-red light-emitting device has also been made by using the LLS:0.3%Mn⁴⁺,1.6%Mg²⁺ phosphors and a 365 nm emitting InGaN chip, which can emit far-red light that is visible to the naked eye. Importantly, the emission spectrum of the LLS:0.3%Mn⁴⁺,1.6%Mg²⁺ phosphors can match well with the absorption spectrum of phytochrome P_FR, indicating the potential of these phosphors to be used in plant growth light-emitting diodes.

Double perovskite La₂LiSbO₆:Mn⁴⁺,Mg²⁺ far-red emitting phosphors with internal quantum efficiency as high as 92% and good thermal stability were developed for plant growth LEDs.     

Lu3+ doping induced photoluminescence enhancement in novel high-efficiency Ba3Eu(BO3)3 red phosphors for near-UV-excited warm-white LEDs

Ba₃Eu(BO₃)₃ (BEB) and Lu³⁺ doped BEB red phosphors were successfully synthesized by a high-temperature solid-state reaction method. X-ray diffraction (XRD) patterns, excitation and emission spectra, decay lifetimes, CIE coordinates, internal quantum efficiency, and thermal stability of these phosphors were systematically studied. Under 395 nm excitation, these phosphors exhibited high-brightness red emissions centred at 611 nm. In addition, it was found that doping appropriate amounts of Lu³⁺ ions into BEB phosphors can improve their photoluminescence intensity and internal quantum efficiency. The integrated emission intensity of BEB:0.3Lu³⁺ phosphor was about 1.34 times that of BEB phosphor. Compared with commercial red phosphor Y₂O₂S:Eu³⁺, BEB:0.3Lu³⁺ phosphor showed better color purity (91.4%) and higher emission intensity (about 3.25 times). Surprisingly, the BEB:0.3Lu³⁺ phosphor had a high internal quantum efficiency of almost 87%, which was higher than that of 83% for BEB phosphors. Meanwhile, the BEB:0.3Lu³⁺ phosphors also exhibited good thermal stability with activation energy around 0.14 eV, and the integrated emission intensity at 423 K remained about 52% of that at 303 K. Finally, by using commercial BaMgAl₁₀O₁₇:Eu²⁺ blue phosphors, commercial (Ba,Sr)₂SiO₄:Eu²⁺ green phosphors, as-prepared BEB:0.3Lu³⁺ red phosphors and a 395 nm near-ultraviolet-emitting light-emitting diode (LED) chip, a prototype warm white LED device was fabricated, which showed good color rendering index (CRI = 84.7) and low correlated color temperature (CCT = 3377 K).

Novel high-efficiency Ba₃Eu(BO₃)₃:Lu³⁺ red phosphors with internal quantum efficiency as great as 87% were developed for near-UV-excited warm-white LEDs.     

Metabolic profiling of isomeric aglycones central‐icaritin (c‐IT) and icaritin (IT) in osteoporotic rats by UPLC‐QTOF‐MS

The isomers, although of similarly chemical structures, have different pharmacological activities due to their metabolic processes in vivo. Central‐icaritin (c‐IT) and icaritin (IT) are isomers and major bioactive aglycones of the Herba Epimedii. In this study, we found that the anti‐osteoporotic effect of c‐IT was stronger than IT on bone structural changes in osteoporotic rats evaluated by Micro‐μCT with the parameters of bone mineral density (BMD), bone mineral content (BMC), tissue mineral content (TMC), and tissue mineral density (TMD). c‐IT treatment significantly increased the bone microarchitecture, compared with IT (p < 0.05). In order to explain their differences in anti‐osteoporosis, the metabolic profiling and pathways of c‐IT and IT in the plasma, bile, urine, and faeces of ovariectomized (OVX) rats were investigated by ultra‐performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC‐QTOF‐MS) after oral administration of c‐IT or IT (80 mg/kg). Finally, 59 metabolites of c‐IT and 43 metabolites of IT were identified by elucidating their corresponding quasimolecular ions and fragment ions. IT could be quickly absorbed into blood and reached a maximum plasma concentration, and then be rapidly conversed to its glucuronidation metabolites, most of which were excreted out by urine. Interestingly, the absorbed and conjugated speeds of c‐IT were slower than IT. The metabolic processes of c‐IT existed enterohepatic circulation, which decreased the metabolism and excretion rate of c‐IT, and prolonged the anti‐osteoporosis effect. Our findings provided evidence on the difference on metabolic profiles of c‐IT and IT in osteoporotic rats, which might shed new lights on improving anti‐osteoporotic effects of IT and c‐IT. Copyright © 2014 John Wiley & Sons, Ltd.     

(R)-2-Phenylpyrrolidine Substituted Imidazopyridazines: A New Class of Potent and Selective Pan-TRK Inhibitors

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Effects of Maillard-type caseinate glycation on the preventive action of caseinate digests in acrylamide-induced intestinal barrier dysfunction in IEC-6 cells

Dietary acrylamide has attracted widespread concern due to its toxic effects; however, its adverse impact on the intestines is less assessed. Protein glycation of the Maillard-type is widely used for property modification, but its potential effect on preventive efficacy of protein digest against the acrylamide-induced intestinal barrier dysfunction is quite unknown. Caseinate was thus glycated with lactose. Two tryptic digests from the glycated caseinate and untreated caseinate (namely GCN digest and CN digest) were then assessed for their protective effects against acrylamide-induced intestinal barrier dysfunction in the IEC-6 cell model. The results showed that acrylamide at 1.25–10 mmol L⁻¹ dose-dependently had cytotoxic effects on IEC-6 cells, leading to decreased cell viability and increased lactate dehydrogenase release. Acrylamide also brought about barrier dysfunction, including decreased trans-epithelial electrical resistance (TEER) value and increased epithelial permeability. However, the two digests at 12.5–100 μg mL⁻¹ could alleviate this dysfunction via enhancing cell viability by 70.2–83.9%, partly restoring TEER values, and decreasing epithelial permeability from 100% to 76.6–94.1%. The two digests at 25 μg mL⁻¹ strengthened the tight junctions via increasing tight junction proteins ZO-1, occludin, and claudin-1 expression by 11.5–68.6%. However, the results also suggested that the GCN digest always showed lower protective efficacy than the CN digest in the cells. It is concluded that Maillard-type caseinate glycation with lactose endows the resultant tryptic digest with impaired preventive effect against acrylamide-induced intestinal barrier dysfunction, highlighting another adverse effect of the Maillard reaction on food proteins.

Glycated caseinate digest of the Maillard-type has lower protective action than caseinate digest against acrylamide-induced barrier dysfunction in IEC-6 cells.