原位杂交法检测卵巢上皮癌中诱导型一氧化氮合酶mRNA

目的 :研究卵巢上皮性癌诱导型一氧化氮合酶 (iNOS)的转录水平。方法 :原位杂交法检测 71例卵巢上皮性癌 ,13例卵巢良性肿瘤及 11例正常卵巢组织中iNOSmRNA表达水平。结果 :卵巢上皮性癌中iNOSmRNA高于正常卵巢组织和卵巢良性肿瘤 ,P<0 .0 5。但卵巢上皮性癌Ⅲ ,Ⅳ期与Ⅰ ,Ⅱ期比较无显著差异 ,P>0 .0 5。分化程度比较 ,中、低分化组明显高于高分化组 ,但中、低分化组织间差异无显著性。结论 :诱导型一氧化氮合酶转录水平的升高 ,是引起诱导型一氧化氮合酶水平升高的主要原因 ,在卵巢上皮性癌发生、发展中发挥一定作用     

纤维胆道镜370例取石体会

目的 进一步提高纤维胆道镜结石取净率，从而提高胆道结石治愈率。方法 总结10年370例纤维胆道镜取石体会，归纳出“网、拉、冲、扩、咬、推”六种操作技巧。结果 对370例胆道结石术后残留结石病人共取石537例次，完全取净率达89．5％，基本取净率8．4％，取石失败2．1％。结论 纤维胆道镜正确灵活的操作方法可避免取石过程中不必要的损伤，缩短操作时间，避免遗漏结石并能取出困难部位的结石。     

胰岛素样生长因子-Ⅰ对软骨细胞凋亡的抑制

目的:探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对软骨细胞增殖及白细胞介素-1(IL-1)诱导软骨细胞凋亡的影响,揭示其抗损伤作用机制,为关节软骨损伤治疗提供理论依据。方法:分离培养人胚胎关节软骨细胞,采用四氮甲基唑蓝(MTT)法测定不同含量软骨细胞增殖活性的变化,利用光镜、电镜、DNA电泳及流式细胞仪测定作为凋亡检测指标。结果:IGF-Ⅰ呈剂量依赖式促软骨细胞增殖,当IGF-Ⅰ含量达50μg/L时,促软骨细胞增殖作用达最大值。IL-1组光镜、电镜下可见典型的细胞凋亡形态学改变,琼脂糖凝胶电泳示特征性的DNA梯状条带,IGF-Ⅰ处理组未见明显凋亡征象。流式细胞仪检测发现,IGF-Ⅰ处理后软骨细胞凋亡率显著降低。结论:IGF-Ⅰ能促进软骨细胞增殖,对IL-1诱导的软骨细胞凋亡具有保护作用。     

Restoration of disturbed respiration in cats by thyrotrophin releasing hormone

Department of Physiology of Man and Animals, Biological Faculty, M. V. Lomonosov Moscow University. (Presented by Academician of the Academy of Medical Sciences of the USSR I. P. Ashmarin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 106, No. 7, pp. 17–19, July, 1988.     

Trehalose protects against ocular surface disorders in experimental murine dry eye through suppression of apoptosis

The disaccharide trehalose is a key element involved in anhydrobiosis (the capability of surviving almost complete dehydration) in many organisms. Its presence also confers resistance to desiccation and high osmolarity in bacterial and human cells by protecting proteins and membranes from denaturation. The present study used a novel murine dry eye model induced by controlled low-humidity air velocity to determine whether topically applied trehalose could heal ocular surface epithelial disorders caused by ocular surface desiccation. In addition, the efficacy of 87.6 mM trehalose eyedrops was compared with that of 20% serum, the efficacy of which has been well documented. Mice ocular surface epithelial disorders were induced by exposure of murine eyes to continuous controlled low-humidity air velocity in an intelligently controlled environmental system (ICES) for 21 days, which accelerated the tear evaporation. The mice were then randomized into three groups: the control group received PBS (0.01M) treatment; a second group received 87.6 mM trehalose eyedrops treatment; and the third group received mice serum eyedrops treatment. Each treatment was administered as a 10 μl dose every 6 h for 14 days. The resultant changes in corneal barrier function and histopathologic examination of cornea and conjunctiva were analyzed and the level of apoptosis on the ocular surface was assessed using active caspase-3. After 14 days of treatment, the corneal fluorescein staining area, the ruffling and desquamating cells on the apical corneal epithelium, as well as the apoptotic cells on ocular surface epithelium had significantly reduced in eyes treated with trehalose compared with those treated with serum and PBS. In contrast, after 14 days of treatment, improvements in the thickness of the corneal epithelium, the squamous metaplasia in conjunctival epithelium and the number of goblet cells of the conjunctiva were less marked in eyes treated with trehalose compared with serum. These results demonstrated that trehalose could improve the appearance of ocular surface epithelial disorders due to desiccation through suppression of apoptosis. Trehalose produces some of the same responses as serum upon topical application and can maintain corneal health.     

99Tcm-MIBI动力学变化与大鼠缺血-再灌注心肌存活的相关性研究

目的探讨用99Tcm-甲氧基异丁基异腈(MIBI)动力学变化评价心肌存活的价值.方法15只离体Krebs-Henseleit(KH)液灌注的鼠心脏,随机分成3组对照组(5只),有葡萄糖的缺血-再灌注组(IR+G组,5只),无葡萄糖的缺血-再灌注组(IR-G组,5只).用含99Tcm-MIBI(14.8MBq)的KH液灌注,观察40min的摄取和清除.用肌酸激酶(CK)分析、氯化三苯四唑(TTC)染色和透射电镜(TEM)分析研究心肌损伤程度,用放射自显影(ARG)观察99Tcm-MIBI在心肌内的分布.结果99Tcm-MIBI的摄取[每克组织百分注射剂量率(%ID/g)]在IR+G组为(7.09±0.97)%ID/g,IR-G组为(6.64±0.68)%ID/g,对照组为(11.44±1.79)%ID/g,IR-G组与IR+G组相比摄取量差异无显著性(P＞0.05),IR-G组和IR+G组与对照组相比均显著降低(P＜0.05).IR-G组99m-MIBI清除分数为(72.75±9.89)%,远高于对照组[(20.68±1.92)%]和IR+G组[(21.03±3.68)%,P均＜0.05],对照组与IR+G组的差异无显著性.99Tcm-MIBI的40min清除末滞留率在IR-G组[(1.82±0.73)%ID/g]和IR+G组[(5.61±0.89)%ID/g]远小于对照组[(9.09±1.57)%ID/g,P＜0.05],IR-G组也远小于IR+G组(P＜0.001).CK分析、TFC染色和TEM分析证明IR-G组比IR+G组有更多的心肌损伤.通过TTC染色(r=0.84,P＜0.05)和CK分析(r=-0.97,P＜0.05)确定最终99Tcm-MIBI的活度与存活心肌量高度相关,通过ARG证实99Tcm-MIBI分布于鼠心肌细胞及间质内(光镜下).结论99Tcm-MIBI的清除对代谢状态敏感,可用于评价进行性心肌损伤.     

The use of acoustic microscopy to study the mechanical properties of glass-ionomer cement.

OBJECTIVES: The aim of the study was to investigate the relationship between microstructure, acoustic and mechanical properties of hardened dental cement samples, prepared with different powder/water ratios. METHODS: Glass-ionomer dental cement samples, prepared with a standard amount of cement powder and different amounts of water have been examined after being hardened. Surface microstructure and ultrasound, longitudinal and shear velocities were obtained with a scanning acoustic microscope. Conditional effective elastic modulus and Poisson's ratio have been calculated using longitudinal and shear sound velocity values. Then on the same samples elastic modulus and microhardness have been determined by standard tests. Additional samples have been used to determine compressive strength. RESULTS: Density; conditionally instantaneous elastic modulus; high-elasticity modulus and compression strength of the samples decrease when large amounts of water were used for their preparation. At the same time porosity and microhardness of the cement matrix increase. Acoustic parameters and parameters of elasticity, calculated on the basis of sound velocity, demonstrated changes, similar to those obtained in standard mechanical tests. SIGNIFICANCE: The established relation between microstructure, acoustic and mechanical parameters demonstrated a high capacity of acoustic microscopy application for non-destructive characterization of dental materials. A particular advantage of the acoustic microscopy is the opportunity to evaluate microstructure and mechanical properties on the same sample.     

Radiopharmaceuticals for radionuclidic diagnostics of bone pathology (review)

This review gives a brief outline of the history of development of radiopharmaceuticals (RPh) for radionuclide diagnostics of bone pathology. The advantages of RPh based on ^99mTc-diphosphonates are demonstrated.     

Substituted Amides and Hydrazides of Dicarboxylic Acids. Part 15. Synthesis and Hypoglycemic Activity of Some Amides and Acylhydrazides of Tetrachlorophthalic Acid

Pharmaceutical Chemistry Journal -