No association between thrombospondin-4 A387P polymorphism and acute coronary syndrome in Chinese Han population

Objective: The thrombospondin-4 (TSP-4) gene G29926C (A387P) polymorphism was recently reported to be associated with an increased risk of MI (myocardial infarction) in American population. However, several subsequent studies produced controversial findings. The aim of this study was to explore the possible association between TSP-4 A387P polymorphism and ACS (acute coronary syndrome) in Chinese Han population. Methods :A case-control study including 412 patients with ACS and 337 controls free from CAD (coronary artery disease) was conducted. TSP-4 A387P polymorphism was determined by PCR (polymerase chain reaction) and RFLP (restriction fragment length polymorphism) analysis. Results:Slightly decreased frequency of GC genotype was observed in patients with ACS, compared with controls (5.3% vs. 7.1%), but the difference did not reach statistical significance (P = 0.31 ). Similarly, the prevalence of C allele was 2.7% and 3.6% for ACS and control groups, respectively (P = 0.32). None of homozygote was detected for C allele. Further analyses in subjects subgrouped according to sex and age also showed no association of TSP-4 A387P polymorphism with ACS. Furthermore, after adjustment for conventional risk factors by multiple logistic regression analysis, the carrier prevalence of C allele did not differ significantly between ACS and control groups (OR = 0.85; 95% CI:0.45-1.59; P = 0.60). Conclusion:The present study suggested that the TSP-4 A387P variant showed a low prevalence compared with western populations and failed to associate with an altered risk of ACS in Chinese Han population. The findings further supplement experimental data for TSP-4 gene study of coronary disease.     

Purified protein derivative skin testing on HIV/AIDS patients and logistic regression analysis of its risk factors

Objective: To understand the reactivity of purified protein derivative skin test(PPD test) in HIV-infected persons and to determine the influential factors associated with PPD. Methods: 174 HIV/AIDS patients registered in the local center for disease control and prevention(CDC) participated this study from April to June in 2006. Questionnaire, CD4 count and thoracic roentgenogram were performed for all participants. Results: In this study, response rate of questionnaires was 83.65%. The majority of these participants had a different degree of immunodeficiency that accounted for 93.64%. Female patients had a higher CD4 count than that of males. The total positive rate of PPD was 38.15%. Analysis of single factor in our study indicated that CD4 count, previous tuberculosis history, tuberculosis contact history and thoracic roentgenogram manifestation of patients were related to their PPD diameters. Further analysis of multiple factors also supports the previous conclusion that CD4 count and previous tuberculosis history of patients were risk factors in the PPD test. Conclusion: The PPD test of HIV/AIDS patients could be affected by several factors. For persons infected with HIV, the confirmation of latent tuberculosis infection (LTBI) should be considered the combination effect of previous MTB infection and body cellular immune function.     

2012-2014年广东省哨点医院诺如病毒GII.4 Sydney变异株流行状况及暴发疫情特征分析

目的：分析2012年1月至2014年6月广东省哨点医院诺如病毒GII.4 Sydney变异株流行状况,以及由诺如病毒GII.4 Sydney变异株感染引起的暴发疫情特征。方法2012年1月至2014年6月在广东省选取22家医院的门诊科室为监测哨点,将采集的腹泻病例粪便样本（共10750份）送至市级CDC,进行病毒核酸提取及诺如病毒核酸检测,所有阳性样本递送至广东省CDC,并按照随机数字法抽取了855份进行诺如病毒基因分型,共成功测序样本690份。采用χ2检验比较不同年龄组、不同时期内腹泻病例诺如病毒感染情况。通过广东省突发公共卫生事件管理信息系统,收集2012年1月至2014年6月广东省13起由诺如病毒GII.4 Sydney变异株感染引起的社区暴发疫情数据,进行社区流行病学分析。结果2012年8月首次检出诺如病毒GII.4 Sydney变异株,2012年11月检出比例为13/15。2012年11月至2013年1月（T1时期）,各月份中腹泻病例诺如病毒感染阳性率分别为23.8%（100/421）、15.9%（61/383）、19.2%（95/495）,2013年11月至2014年1月（T2时期）,各月份中腹泻病例诺如病毒感染阳性率分别为17.0%（90/529）、8.7%（37/426）、11.2%（46/409）,均低于T1时期（χ2值分别为6.65、9.93、10.74,P值分别为0.010、0.002、0.001）。T1时期内≥15、<15岁组腹泻病例诺如病毒感染阳性率分别为26.3%（143/543）、14.9%（113/756）（χ2=25.90,P<0.001）;T2时期≥15、<15岁组阳性率分别为10.1%（52/516）、14.3%（121/848）（χ2=5.09,P=0.024）。13起暴发疫情中,食源性传播占10/13。结论广东省2012年8月首次检出诺如病毒GII.4 Sydney变异株,2012年11月起呈现社区流行,流行1年后,强度降低;由诺如病毒GII.4 Sydney变异株引起的暴发疫情主要以食源性传播为主。     

一氧化氮合酶和血红素氧合酶与原发性肝癌肿瘤血管形成的关系

目的:研究诱导型一氧化氮合酶(Nitric Oxide Synthase,inducible,iNOS)和诱导型血红素氧合酶(Heme Oxygenase 1,HO-1)在人原发性肝癌中的表达及它们与肝癌肿瘤血管形成的关系.方法:采用免疫组化S-P法检测56例原发性肝癌患者肝癌组织,17例尸检正常肝组织中iNOS和HO-1的表达,同时以抗CD34标记内皮细胞,检测微血管密度(Microvessels Density,MVD),并分析iNOS和HO-1与肿瘤血管形成之间的关系.结果:肝癌组织中的iNOS阳性表达率为94.6%,MVD均值为144.30±15.30,显著高于正常肝组织.肝癌组织中HO-1的阳性表达率为89.3%,MVD均值为133.60±13.90,显著高于正常肝组织.结论:肝癌组织中的iNOS和HO-1高阳性表达,两者呈正相关.iNOS和MVD呈正相关,提示了iNOS的表达促进了肝癌组织的肿瘤血管的形成.     

急性肺动脉栓塞29例临床分析

目的对急性肺栓塞进行临床分析,探讨急性肺栓塞的危险因素、临床表现、诊断和治疗。方法对2006年1月～2011年1月诊断为PE的29例患者的危险因素、临床表现、辅助检查及治疗情况进行分析。结果 PE缺乏特异性临床表现,但临床症状和体征能提供重要线索。心电图,超声心动图,X线胸片,动脉血气分析,双下肢深静脉血管超声、CT肺动脉造影,均能为PE早期诊断提供相对特征性改变,早期溶栓和抗凝治疗可有效降低病死率,改善预后。结论提高PE的早期诊断率,尽早溶栓和抗凝治疗是治疗成功、改善预后的关键。     

Epidemiological and morbidity assessment of Schistosoma japonicum infection in a migrant fisherman community, the Dongting Lake region, China

We assessed the epidemiology and morbidity related to Schistosoma japonicum infection by both parasitological (Kato-Katz method and miracidium hatching test) and ultrasound examination in a migrant fisherman community (n = 106) from the Dongting Lake region in China in 2001. A prevalence of 69.8% and a mean infection intensity of 66.5 eggs per gram (epg) were recorded for this group. Males had a higher level of infection (77%) and intensity (92.2 epg) compared with females (58% and 28.6 epg, respectively). Subjects aged 11-20 years had the highest prevalence of infection (91%) and the heaviest infection intensity (85.3 epg) among all age groups. Twenty-six percent of the subjects had not been treated previously for schistosomiasis. Parenchymal fibrosis (> or = stage II) detected by ultrasound and spleen enlargement (or splenectomy) occurred in 37% and 9% of subjects, respectively. Portal vein dilation was detected in 18% subjects, and 58% (11/19) of those patients with portal vein enlargement were shown to have parenchymal fibrosis (> or = stage II). These results indicate that S. japonicum infection and related morbidity in mobile fisherman is still unacceptably high despite the fact that China has implemented a Schistosomiasis Control Project financed by a World Bank Loan for a period of 8 years since 1992. Clearly, new approaches for schistosomiasis control, especially in communities like these mobile fishermen, should be explored.     

成都市知晓自身感染艾滋病病毒的男男性行为人群无保护肛交行为研究

目的 了解成都市知晓自身HIV阳性的MSM人群无保护肛交行为（UAI）的相关影响因素。方法 2015年采用方便抽样在成都招募已接受随访服务且知晓自身HIV阳性≥6个月的MSM,采用问卷调查收集MSM最近6个月性行为情况及相关信息。采用logistic回归进行单因素和多因素分析。结果 共招募知晓自身HIV阳性的MSM 330人,实际调查201人,最近6个月的UAI发生率为18.41%（37/201）。多因素logistic回归分析显示,最近1个月肛交次数≥3次（OR=6.22,95% CI：1.88～20.56）、初中及以下文化程度（OR=7.29,95% CI：1.36～39.16）、已婚/离异/丧偶（OR=4.65,95% CI：1.13～19.17）、与同性同居（OR=3.32,95% CI：1.01～10.95）是可能发生UAI的相关因素。结论 频繁同性肛交、初中及以下文化程度、已婚/离异/丧偶、与同性同居是知晓自身HIV阳性的MSM人群发生UAI的相关因素。     

军队鼠害综合防治技术规范的制定

通过查阅分析相关文献、现场调研、征求专家意见及会议审定研究制定军队标准《军队鼠害综合防治技术规范》。该标准规范了军队鼠害综合治理,为依法开展鼠害防治工作提供技术支持。     

Experimental and Numerical Study on the Penetration Performance of a Shaped Charge

In guided ammunition, because a shaped energy jet warhead is located behind the control cabin (including the guidance cabin, the steering gear cabin, and the flight control cabin), the penetration order of a shaped energy jet is the control cabin and the target plate. In order to obtain maximum penetration depth by a shaped energy jet into a Q235 steel plate, the penetration performance of shaped energy jets was studied by numerical simulation and experimental verification. Firstly, the penetration performance of a warhead under different conditions at a certain explosion height is studied, which is the penetration performance of a Q235 steel plate with and without the control cabin. Secondly, the numerical simulation results are verified by experimental method. The numerical simulation and experimental results showed that, after penetration of the shaped energy jet warhead into the control cabin, it continued to penetrate the 20 mm-thick Q235 steel plate. At a certain explosion height, the maximum penetration depth of the shaped energy jet warhead into the Q235 steel plate was about 80 mm. Alongside the numerical simulation and experiment, the armor-breaking process of the shaped charge jet was analyzed theoretically. The results show that when the shaped energy jet warhead is located behind the control cabin, although the control cabin will have a certain impact on the penetration ability of shaped energy jet, the penetration performance of the residual jet still has the ability to penetrate light armor.     

睡眠剥夺对精神-运动操作功能的影响

目的：探讨５６ｈ睡眠剥夺对精神－运动操作功能的影响。了解长时间连续睡眠剥夺中的认知功能下降程度,为制定预防措施提供科学依据。方法：选择２０名健康男性青年,采用韦氏成人智力检测的数字符号和成人神经心理检验的手指叩击测验、手指辩认和指尖认字进行测验。结果：对照组无睡眠剥夺下,经过学习迁移作用。实验２４ｈ和实验５６ｈ对数字符号后反速度以及５６ｈ右手手指叩击速度均明显加快（Ｐ〈０．０１）;实验组因睡眠剥夺