The Influence of Precipitate Morphology on the Growth of Austenite Grain in Nb-Ti-Al Microalloyed Steels

The present study investigates the morphological evolution of carbonitrides and the effect of these precipitates on grain boundary pinning during pseudo-carburizing a Nb-Ti-Al microalloyed steel. The result indicated that three kinds of complex precipitates with different morphologies containing Nb, Ti, and Al respectively were observed in samples austenitized at different temperatures and times. The NbC and TiN precipitates played an important role in pinning grain boundaries and suppressing the growth of austenite grains, relying on the high thermal stability of TiN precipitates and small size of NbC precipitates. The precipitate characteristics affected the size of austenite grain. Based on the Zener pinning model, the effect of precipitate characteristic on austenite grain size was quantitatively analyzed. It is found that the existence of NbC and TiN precipitated at high temperature makes austenite grain growth difficult when austenite grain boundaries were pinned by fine and diffused precipitates.     

111例侵袭性肺曲霉病(IPA)的临床特征及预后风险因素

目的 探讨侵袭性肺曲霉病(IPA)的临床特征、实验室检查及预后风险因素。方法 回顾性分析2020年9月—2023年6月某院IPA患者的临床资料,分析其临床特征及预后风险因素。结果 共纳入111例IPA患者,年龄为(68.8±12.5)岁,以男性为主(63.1%),主要分布于呼吸科、重症监护病房(ICU)、血液科及感染科,共占75.6%。IPA常见的曲霉为烟曲霉、黄曲霉及黑曲霉,分别占67.6%、19.8%、4.5%。支气管肺泡灌洗液的(1,3)-β-D-葡聚糖抗原检测(G试验)、半乳甘露聚糖抗原检测(GM试验)阳性率分别为73.7%、68.0%。111例IPA患者中,32例(28.8%)随访发现预后不良,25例(22.5%)合并病毒感染。logistic多因素回归分析显示,合并病毒感染[OR(95%CI):4.535(1.385～14.846),P=0.012]、连续3周使用糖皮质激素史[OR(95%CI):9.128(2.293～36.341),P=0.002]、机械通气[OR(95%CI):4.690(1.100～19.990),P=0.037]及留置导尿管[OR(95%CI):7...     

中下面部线雕联合局部注射填充对皮肤状态及不良反应的影响

目的 探究在面部年轻化治疗中采用中下面部线雕联合局部注射填充的效果及对皮肤状态的影响。 方法 选取2021年1月-2024年1月于观山湖科珑小可美容诊所美容皮肤科行面部年轻化治疗的60例患者, 随机分为对照组和观察组,每组30例。对照组采用局部注射填充治疗,观察组采用中下面部线雕联合局 部注射填充治疗,比较两组皮肤状态、心理状态、生活质量及不良反应发生情况。结果 观察组术后皮 肤水分、弹性评分高于对照组,油脂、暗斑评分低于对照组（P ＜0.05）;观察组术后SAS、SDS评分均 低于对照组（P ＜0.05）;观察组术后生活质量各维度评分均高于对照组（P ＜0.05）;观察组不良反应发 生率为6.67%,低于对照组的30.00%（P＜0.05）。结论 中下面部线雕联合局部注射填充在面部年轻化中的 应用效果确切,抗衰效果及美学效果良好,可有效改善皮肤质地,减少皱纹,且治疗后不良反应较少,安 全性较高,有利于改善患者的心理状态及生活质量,值得临床应用。     

MLL5基因敲除对小鼠结肠癌CT26细胞移植瘤生长的影响及其机制

目的：探讨混合谱系白血病5(MLL5)基因在小鼠结肠癌CT26细胞移植瘤生长中的作用及其分子机制。方法：利用CRISPR/Cas9技术构建MLL5基因缺失、MLL5和DDX58双基因缺失的结肠癌CT26细胞模型,用Sanger测序和WB法验证敲除效果。将基因敲除的CT26细胞接种到野生型BALB/c小鼠和免疫缺陷型NSG小鼠皮下,构建基因缺失结肠癌CT26细胞移植瘤小鼠模型,并观察移植瘤的生长及荷瘤小鼠的总生存期（OS）。结果：在野生型小鼠中,MLL5基因缺失的CT26细胞移植瘤生长速度显著性低于野生型癌细胞移植瘤,并延长荷瘤小鼠的OS(P<0.01);在NSG小鼠中,MLL5基因缺失对CT26细胞移植瘤的生长速度以及荷瘤小鼠的OS没有明显改变。MLL5基因缺失提高了癌细胞中视黄酸诱导基因1(RIG-1)蛋白水平,DDX58基因缺失可逆转MLL5基因缺失在CT26细胞移植瘤中的作用。结论：MLL5基因缺失可提高结肠癌CT26细胞中RIG-1蛋白水平、促进肿瘤免疫,从而抑制荷瘤小鼠肿瘤生长,提示MLL5可能成为结肠癌治疗的新靶点。     

Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR‐494‐3p/LASP1 axis

BackgroundChemoresistance is one of the major obstacles for tumor treatment. Circular RNAs (circRNAs) have been confirmed to play vital roles in chemoresistance of cancer, including esophageal squamous cell carcinoma (ESCC). We investigated the roles and mechanisms of circ_0007142 in cisplatin (DDP) resistance of ESCC.MethodsQuantitative real‐time polymerase chain reaction (qRT‐PCR) was conducted to determine the levels of circ_0007142, DOCK1 mRNA, microRNA‐494‐3p (miR‐494‐3p) and LIM And SH3 Protein 1 (LASP1) mRNA. RNase R assay was conducted to analyze the characteristic of circ_0007142. Cell Counting Kit‐8 (CCK‐8) assay was performed to evaluate IC50 of DDP. Flow cytometry analysis, 5‐ethynyl‐2’‐deoxyuridine (EdU) assay and transwell assay were carried out to examine cell apoptosis, proliferation and invasion, respectively. Dual‐luciferase reporter assay was employed to verify the association between miR‐494‐3p and circ_0007142 or LASP1. Murine xenograft assay was conducted to investigate the role of circ_0007142 in DDP resistant in vivo. The protein level of LASP1 in tumors was measured by Immunohistochemistry (IHC) analysis.ResultsCirc_0007142 was upregulated in DDP‐resistant ESCC tissues and cells. Circ_0007142 knockdown improved DDP sensitivity, induced cell apoptosis and hampered cell proliferation and invasion in DDP‐resistant ESCC cells. Circ_0007142 functioned as the sponge for miR‐494‐3p and miR‐494‐3p inhibition reversed the impacts of circ_0007142 knockdown on DDP resistance, cell apoptosis, proliferation, and invasion. LASP1 was a target of miR‐494‐3p, and the effects on DDP resistance, cell apoptosis, growth, and invasion mediated by LASP1 downregulation were rescued by miR‐494‐3p inhibition. Moreover, circ_0007142 knockdown enhanced DDP sensitivity in vivo.ConclusionCirc_0007142 improved DDP resistance of ESCC by upregulating LASP1 via sponging miR‐494‐3p.     

单侧椎弓根钉内固定建立新型山羊脊柱侧凸模型的实验研究

目的探讨未成年山羊脊柱单侧椎弓根钉内固定并对侧肋骨切除建立新型脊柱侧凸模型的可行性。方法实验对象为14只雌性山羊,年龄5~8周,体重6~8kg。在全麻下进行模型制作,T5~L2做右旁正中皮肤切口,分别于脊柱左侧T6,7和T12、L1部位置入特制椎弓根螺钉,经皮下及肌肉下插入1枚不锈钢棒置入螺钉槽口内,远近端螺钉之间适当加压使脊柱向右凸;然后于脊柱右侧分别切除第7~12肋骨2~3cm左右。术后即刻及每4周拍摄正侧位X线片,观察山羊在生长过程中脊柱弯曲的变化。结果1只小羊因麻醉过量,术中死亡;另1只因术后感染而导致内固定失败,观察终止。其余12只小羊中,11只小羊X线片显示右侧侧凸畸形,并且Cobb角随时间延长而增大。只有1只羊术后Cobb角没有进展。术后即刻Cobb角平均为29.0°(23.0°~38.5°),8~10周后Cobb角增至平均43.0°(36.0°~58.0°),平均进展14.0°。术后8周(1只为10周)时获取脊柱标本,去除内固定后发现所有弯曲均为结构性。从脊椎的旋转和外观方面来看,均与特发性脊柱侧凸畸形相似。结论单侧椎弓根钉内固定是一种比较可靠的建立脊柱侧凸模型的方法,模型与特发性脊柱侧凸相似,适用于脊柱侧凸畸形的实验研究。     

外企员工中患脂肪肝、高血糖、高血压、高脂血症情况的分析

目的了解外企员工中高血压、脂肪肝、血脂、高血糖的情况,为做好预防保健提供参考。方法回顾性统计天津国际旅行卫生保健中心2005-2006年外资企业员工体检结果并进行分析。结果四种疾病检出率男性均高于女性。结论经统计学分析,高脂肪肝、高脂血症性别之间存在显著性差异,而高血压、高血糖无显著性差异。     

5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone up-regulates miR-145 expression and inhibits proliferation of gastric cancer cells

IntroductionGastric cancer is a frequently detected malignancy, and its incidence has increased over the past decades in East Asia. The present study investigated the effect of 5,7,2, 5-tetrahydroxy-8,6-dimethoxyflavone (THDMF) on gastric cancer cells and explored the underlying mechanism. The study analysed cell viability changes, apoptotic features, and metastasis potential of treatment with THDMF.Material and methodsMTT colorimetric assay was used for measurement of MKN28, MKN45, and GES-1 cell proliferation and flow cytometry for the detection of apoptosis. Transwell and wound healing assays were used to observe the invasion and migration abilities of MKN28 cells. The expression of p21, MMP2/-9, PI3K, and c-Myc proteins was detected by western blotting.ResultsThe THDMF treatment significantly (p < 0.05) reduced MKN28 and MKN45 cell proliferation without changing GES-1 cell viability. A significant increase in apoptotic cell population on treatment with THDMF was observed. Treatment of MKN28 cells with THDMF increased the percentage of cells in the G1 phase. Exposure of MKN28 cells to THDMF caused a marked decrease in invasion and migration potential in comparison to control cells. The expression of miR-145 was markedly increased in MKN28 cells on treatment with THDMF. In MKN28 cells expression of c-Myc, PI3K, p-AKT, MMP-2, and MMP-9 was suppressed markedly on exposure to THDMF. The expression of p21 protein in MKN28 cells was markedly promoted on exposure to THDMF.ConclusionsTHDMF exhibits anti-cancer effect on gastric cancer cells in vitro by activation of cell apoptosis and arrest of cell cycle. In addition, THDMF promoted miR-145 expression and down-regulation of PI3K/AKT signalling pathway in MKN28 cells. Therefore, THDMF may be utilised as a potential novel therapeutic agent for the treatment of gastric cancer.     

Greater Adherence to Dietary Guidelines Associated with Reduced Risk of Cardiovascular Diseases in Chinese Patients with Type 2 Diabetes

The evidence regarding the impact of the scores on healthy eating indices on the risk of cardiovascular events among patients with type 2 diabetes (T2D) is limited. As such, in this study, we examined the associations of adherence to the Chinese and American dietary guidelines and the risk of cardiovascular disease (CVD) among Chinese individuals with T2D. We conducted a 1:1 age- and sex-matched case–control study based on a Chinese population. We used a structured questionnaire and a validated 79-item food-frequency questionnaire to collect general information and dietary intake information, and calculated the Chinese Healthy Eating Index (CHEI) and the Healthy Eating Index-2015 (HEI-2015). As participants, we enrolled a total of 419 pairs of hospital-based CVD cases and controls, all of whom had T2D. We found a significant inverse association between diet quality scores on the CHEI and HEI-2015 and the risk of CVD. The adjusted odds ratios (95% confidence interval) per five-score increment were 0.68 (0.61, 0.76) in the CHEI and 0.60 (0.52, 0.70) in the HEI-2015. In stratified analyses, the protective associations remained significant in the subgroups of sex, BMI, smoking status, tea-drinking, hypertension state, dyslipidemia state, T2D duration, and medical nutrition therapy knowledge (all p < 0.05). These findings suggest that a higher CHEI or HEI-2015 score, representing a higher-quality diet relative to the most recent Chinese or American dietary guidelines, was associated with a decreased risk of CVD among Chinese patients with T2D.     

化脓性脊柱炎的MRI表现分析(附22例报告)

目的评价化脓性脊柱炎的MRI影像学表现。方法回顾性分析22例脊柱椎体感染病例的MR表现,全部病例均经临床随访或手术病理证实。结果发生于颈椎2例,胸椎5例,腰骶椎15例。受累椎体和椎间盘在T1W1呈广泛的融合的低信号,椎体和椎间盘分界模糊不清;而在T2W1图像上,受累椎体与椎间盘的信号强度增高,椎间盘残留部分呈略低或略高的信号。去脂序列中呈等高信号。累及附件、椎体旁软组织肿块形成、硬膜脓肿形成局部硬膜囊受压,相应脊髓受压见于部分病例中。结论MRI影像学对化脓性脊柱炎有明确的诊断价值,MRI应作为怀疑化脓性脊柱炎的常规检查手段。