Target-dependent axonal sprouting following vagal-hypoglossal nerve anastomosis in cats

Purpose: To investigate the relationships between the axonal sprouting and target neurotization by central neurons after nerve heterocon-nection. Methods: Unilateral (right) vagal-hypoglossal nerve anastomosis (VHA) was performed in adult cats. Following 3-315 days postoperation (dpo), quantitative analyses and ultrastructural changes in the proximal portion of the vagal-hypoglossal heteroconnected nerve as well as the time course of neuronal regeneration were studied. Along with this, horseradish peroxidase (HRP) retrograde tracing technique was used to label the neurons of dorsal motor vagal nucleus (DMV) and nucleus ambiguus (NA) to ascertain if target neurotization was established. Results: The contralateral (left) intact vagus nerve proximal to the level of ansa cervicalis showed an average of 33 +/- 1 myelinated and 74 +/- 4 unmyelinated axons in 727 &mgr;m(2) sectional area of the nerve. In the heteroconnected nerve at the corresponding level just proximal to the anastomosis site, there was a marked increase in the number of small axons sprouting from the unmyelinated nerve fibers between 18 and 25 dpo. The number of these axonal sprouts appeared to decline at 32 dpo but its increase of 131 % was sustained until the late regeneration stage at 315 dpo when compared with the contralateral nerve serving as a control. The mean number of myelinated axons per area unit (727 &mgr;m(2)) was reduced to 18 at 3 dpo but was immediately restored to the normal range at 7 dpo. The retrograde labelling of neurons in both the DMV and NA was first detected at 22 dpo and was progressively increased peaking by about 67 dpo. Conclusions: We conclude that compared with the unmyelinated axons, the myelinated axons may acquire a superior interaction with the new target. Furthermore, the postoperative neurotization of tongue muscles may initiate and facilitate the retraction of the redundant axonal sprouts.     

清开灵注射液抗内毒素作用的实验研究

目的探讨清开灵注射液抗大肠埃希菌O111B4内毒素作用。方法采用细菌内毒素检查法(体外)和兔热原检查法(体内)，对清开灵     

逍遥散加减在皮肤科的应用

逍遥散由当归、白芍、白术、茯苓、炙甘草、柴胡、生姜、薄荷组成,有疏肝解郁,健脾和营之功[1].后世医家在原方基础上加减化裁,广泛应用于临床各科.凡邪在半表半里者,出现肝郁脾虚之证,均可采用,以调其偏胜,扶其不足,使病去人安.笔者临床应用逍遥散治疗肝郁不舒、克伐脾土、脾失健运、气血失和而产生的各种皮肤疾患,收到较好的疗效.     

清肝解毒汤治疗青少年乙型肝炎120例

目的：观察中药清肝解毒汤治疗青少年乙型肝炎的临床疗效。方法：采用清肝解毒汤治疗青少年乙型肝炎120例．1疗程30天。结果：痊愈15例，好转101例，无效4例．总有效率为96．7％。结论：清热解毒活血利湿治疗乙型肝炎属于体质壮实者可明显减轻症状，改善肝功能，降低乙肝病毒血清滴度。     

白鲜皮质量标准研究

目的：为中药白鲜皮制定2005年版药典标准。方法：采用TLC法鉴别、反相高效液相色谱法测定白鲜皮中活性成分末梣酮含量，并对各地产白鲜皮药材的水分、总灰分、酸不溶性灰分、浸出物含量进行测定。结论：本方法操作简单，重现性好，可用于白鲜皮药材的质量控制。     

金瓷修复体制作中的问题分析

目的 研究金属烤瓷修复体制作过程中经常出现的问题及如何补救，探讨提高金瓷修复体修复工艺方面的问题。方法 收集并分析临床近5年制作的580例、共650件金属烤瓷修复体，归纳其在制作中出现的问题。结果 瓷裂及崩瓷18件，占2．76％：气泡15件，占2．31％；铸造缺陷11件，占1．69％；色泽不协调12件，占1．85％。结论 金瓷修复体的加工制作是一个复杂而细致的过程，与技工室制作、临床设计及牙体制备都有密切关系，其中任何一个环节的操作不当都可能导致修复体的失败。     

金桃消石散治疗胆囊结石临床研究

目的:观察金桃消石散治疗胆囊结石的临床疗效及其对胆囊收缩功能的影响。方法:选择胆囊结石患者67例,随机分为治疗组和对照组。治疗组32例,口服金桃消石散;对照组35例,服用胆石通。共治疗4个月,分别于治疗前及治疗后,B超检测胆囊结石及胆囊排空率变化情况。结果:治疗组痊愈6例,显效9例,有效15例,无效2例;对照组痊愈2例,显效6例,有效18例,无效9例,两组治疗后胆囊排空率均较治疗前好转,但治疗组与对照组比较,疗效更为显著。结论:金桃消石散可以明显改善胆囊的收缩功能,是治疗胆囊结石有效的方剂。     

生麦散对失血性休克家兔血浆内皮素和肿瘤坏死因子等的影响

目的:探讨生麦散(SMS)对失血性休克的治疗作用.方法:观察生麦散对失血性休克家兔休克前后血浆内皮素(ET)、肿瘤坏死因子(TNF)、β-内啡肽(β-EP)、一氧化氮(NO)含量及一氧化氮合酶(NOS)活性的影响.结果:生麦散能明显降低失血性休克家兔血浆ET、TNF、β-EP、NO水平及NOS活力的升高(P＜0.05).结论:生麦散可通过调节ET、TNF、β-EP、NO水平及NOS活力等作用治疗失血性休克.     

奥利司他对肥胖IGT人群糖尿病预防效果

目的 探讨奥利司他(Orlistat,赛尼可)对肥胖糖耐量低减(IGT)患者的干预效果。方法 患者随机分两组奥利司他治疗组(50例)和对照组(51例),治疗为期1年,于治疗前后测量血脂、口服糖耐量试验(OGTT)、体重、身高等指标。结果1年后,治疗组上述指标明显改善(P<0.01),其恶化为糖尿病的发病率明显低于对照组(P<0.01)。结论 在肥胖IGT干预治疗中,奥利司他加饮食和运动治疗后可使体重减轻,改善糖耐量,明显减少糖尿病的发生。     

Duchenne�ͽ����Լ�Ӫ�������ٴ��ͻ������97������

??Objective??To analyze the clinical and gene mutation characteristics of Duchenne progressive muscular dystrophy ??DMD????summarize the gene mutation hotspots in 97 cases and to explore the correlation between clinical manifestations and genotype. Methods??Totally 97 patients with DMD diagnosed by genetic examination from January 2014 to 2018 were collected and analyzed. The clinical manifestations??serum analyses and gene mutation results were analyzed. Results??The main clinical manifestations of 97 patients??96 boys?? were feeding difficulties?? increased muscle enzyme and limb weakness. Creatine kinase??CK???? lactate dehydrogenase??LDH?? and aspartate aminotransferase??AST?? muscle enzymes were significantly increased. By combining deep-sequencing technologies??the large deletions of DMD gene mutation was in 62 cases??63.92%????there were 11 cases??11.34%?? of large duplication mutation??and 24 cases??24.74%?? of point mutation. All of the mutations could occur in any position in the DMD gene??but there were two hot spots??45 cases were located in the central region gene exon 45??55??72.58% ????12 cases of deletion mutation were located in 5’exon end exon 2??19 area??19.35%??. Conclusion??The main clinical manifestations of the DMD children are feeding difficulty??increased muscle enzyme and limb weakness. The patients with significantly increased muscle enzyme should receive a timely defection of DMD gene.