145例患儿血清中人类微小病毒B19-VP2-IgM的检测

目的 :探讨人类微小病毒B19(HumanParvovirusB19)在患儿中的感染情况。 方法 :用ELISA法对14 5例患儿 (大部分来自血液组 )和 4 8例健康儿童 (来自儿保体检门诊 ,对照组 )的血清标本进行了B19 VP2 IgM检测。 结果 :B19 VP2 IgM阳性检出率ITP和AA最高 ,分别为 4 4 .4 % (12 / 2 7)和 4 0 % (10 / 2 5 ) ,与对照相比差异有统计学意义 (P <0 .0 5 ) ,其它病组与对照组相比差异无统计学意义 (P >0 .0 5 )。 结论 :儿童对B19有较高的感染率 ,尤其ITP和AA患儿与B19感染关系更为密切。     

年轻宫颈癌患者324例临床分析

目的:探讨新疆维吾尔族及汉族年轻宫颈癌患者的临床、病理特点及诊治方法。 方法:对 2 6 4例≤ 35岁的维吾尔族年轻宫颈癌患者及 6 0例汉族年轻宫颈癌患者的临床资料进行回顾性分析。 结果:维吾尔族年轻宫颈癌患者占同期住院的维吾尔族宫颈癌的 14 .0 % (平均值 ) ,其构成比明显高于汉族 (10 .1% ) ,结婚及初孕年龄小于汉族 ,生育次数高于汉族 ,5 0 %以上患者出现阴道流水 ,宫颈肿瘤形态以菜花型为主 ,中晚期多见 ,维吾尔族年轻宫颈癌患者症状出现到就诊时间明显长于汉族患者 ,多采取以放疗为主的综合治疗。 结论 :维吾尔族年轻宫颈癌的症状及治疗方法的选择与宫颈癌的期别有关 ;卫生知识宣传及性健康教育的普及、有效的普查措施对宫颈癌的早期诊断及治疗有重要意义。     

Reduced expression of members of the mhc-i antigen processing machinery in ethnic Uighur women with cervical cancer in the Xinjiang region of China

Objective

Cervical cancer is a major cause of mortality in Uighur women compared with Han women in the Xinjiang region of China. Although a reduction in the class i major histocompatibility complex (mhc-i) antigen processing machinery (apm) is associated with the development of cervical cancer, the mhc-i apm has not been studied in this particular group of women, who have the highest incidence rate of cervical cancer in China.

Study Design

We used immunohistochemical staining and polymerase chain reaction amplification of viral dna from infection with the human papilloma virus (hpv) to study the expression of members of the mhc-i apm in cervical cancer sections collected from Uighur and Han women and in cervicitis samples from age-matched counterparts.

Results

Expression of the molecules of interest was compared between two ethnic groups, and expression of transporter associated with antigen processing 1 and 2, heat shock protein 90, and calnexin were found to be reduced even more significantly in Han women with cervical cancer than in Uighur women with same disease. However, compared with Han women, Uighur women had a higher rate of infection with hpv 16.

Conclusions

The mhc-i apm were reduced in cervical cancer, with heterogeneity in the two ethnic groups. The reduction was more pronounced in Han women, who less frequently had hpv 16 infection, suggesting possible differences in the roles of members of the mhc-i apm and in the mechanisms of cervical cancer development in these two ethnic groups despite residence in the same region of China.     

女性盆底肌力检测及临床分析

背景：了解女性盆底功能障碍性疾病在新疆多民族聚集区的发病相关因素、探寻能相对准确反映盆底功能的量化检查方法,对此病的三级预防起到不可或缺的作用。 目的：探讨女性盆底肌力的相关因素,并分析盆底肌力测定与女性盆底功能障碍性疾病临床症状、体征的相关性及临床意义。 方法：通过问卷调查、体格检查、盆底肌力检测收集2010年4至11月于新疆医科大学第一附属医院妇科住院女性患者资料共210例。采用秩和检验对分组变量进行分析,对有统计学意义的资料再进行Spearman秩相关分析。 结果与结论：年龄、体质量指数、不同分娩次数、绝经、慢性便秘持续时间与盆底肌力呈负相关性;压力性尿失禁症状、盆腔器官脱垂体征与盆底肌力均存在负相关性。提示：控制体质量,减少慢性便秘的发生;做好围绝经期激素替代治疗;从产科因素控制孕产妇盆底组织受损程度,引起患者对自身盆底肌力的重视,利于盆底功能障碍性疾病提早预防及治疗。     

地锦草提取物对红色毛癣菌酶活性的影响

目的 研究地锦草提取物对红色毛癣菌角鲨烯环氧化酶和β-(1,3)-D-葡聚糖合成酶的影响,探讨其抗真菌作用机制.方法 药物干预红色毛癣菌后分离菌体,按试剂盒要求处理样品,利用酶联免疫法和荧光分光光度法,研究地锦草提取物对红色毛癣菌角鲨烯环氧化酶和β-(1,3)-D-葡聚糖合成酶活性的作用.结果 地锦草提取物256 μg·ml~(-1)时,可显著降低红色毛癣菌中角鲨烯环氧化酶的活性(P＜0.01);不同浓度的地锦草提取物,均可降低红色毛癣菌β-(1,3)-D-葡聚糖合成酶的活性(P＜0.01).结论 地锦草提取物抗真菌机制可能与抑制角鲨烯环氧化酶和β-(1,3)-D-葡聚糖合成酶的活性有关.     

地锦草不同醇浓度提取物体外抗真菌作用研究

目的对地锦草不同醇浓度提取物进行体外抗真菌实验,测定其最小抑菌浓度(MIC)值。方法采用美国临床实验室标准化委员会(NCCLS)推荐的《产孢丝状真菌的液基稀释法抗真菌药物敏感性试验方案》(M38-A),选取地锦草提取物敏感的12株皮肤癣菌按文献方法进行抑菌试验。结果地锦草提取物对红色毛癣菌的MIC范围为512～1 024μg/ml;对石膏样毛癣菌的MIC范围为341～512μg/ml;对许兰、犬小孢子、须毛、疣状、紫色毛癣菌的MIC范围为128～1 024μg/ml。结论地锦草不同醇浓度提取物体外实验对真菌的生长具有抑制作用。     

细胞凋亡与Fas的表达在中晚期宫颈癌同步放化疗中的意义

目的：通过探讨细胞凋亡及Fas的表达，讨论中晚期宫颈癌放疗敏感性的机制，从而为宫颈癌的个体化、优化治疗提供理论依据。方法：选取新疆医科大学附属肿瘤医院2006年1月～2007年6月间60例中晚期宫颈癌患者，随机分为2组，单纯放疗（radiotherapy，RT）组30例给予10Gy放疗前、后取活栓，放化疗（synchronal radiochemotherapv，CRT）组30例先行一次化疗再给予10Gy放疗前、后取活检，采用免疫组织化学法和脱氧核糖核酸转移酶介导的缺口末端标记（TUNEL）技术，分别检测30例单纯放疗和30例放化疗联合宫颈癌患者治疗前、后宫颈肿瘤细胞凋亡率及Fas蛋白的表达。结果：RT组和CRT组完全缓解率分别为50％和90％（P=0．0012）。在治疗过程中，RT组和CRT组凋亡阳性率均增加，分别由33.33％上升到66．67％（P=0．02）和36．67％增加到9333％（P=0．000），差异显著，治疗中CRT组较RT组增加更加明显（P=0．02）。Fas的表达亦增加，分别由33．33％上升到70．0％（P=0．01）和26．67％增加到76．67％，差异显著（P=-0．000），但两组间Fas的表达阳性率无差异（P〉0．05）。两组在治疗中，凋亡的阳性率和Fas的阳性表达密切相关，CRT组较RT组相关性更强（P=0．015，r=-0．755；P=0．027，r=0．423）：结论：中晚期宫颈鳞癌CRT比RT有更好的缓解率，其机制可能是化疗和放疗有协同作用，通过上调Fas蛋白诱导了肿瘤细胞的凋亡。     

An Investigation of the First Case of Human Rabies Caused by a Fox in China in May 2016

This study assesses the causes and prevention measures of rabies through epidemiological investigation and analysis. A field epidemiological survey was conducted to investigate a case of rabies by fox bite. The onset of symptoms began 50 days after the bite. The patient did not receive standard treatment, rabies vaccination, or rabies immunoglobulin injection. The fox was killed on the spot. Saliva and pre-death blood samples were collected at different periods, and only blood RT-PCR tests yielded positive results. Wild fox bite is a major risk factor of rabies infection in Xinjiang Province, China.     

3D打印多通道施源器在宫颈癌术后患者近距离治疗中研究北大核心CSCD

目的比较3D打印多通道施源器与常规阴道单通道施源器用于近距离治疗的剂量学差异,为宫颈癌术后患者的近距离治疗提供一定的指导依据。方法选取25例自2019年1月—2020年11月接受^(192)Ir高剂量率近距离治疗的宫颈癌术后合并阴道上皮内瘤变Ⅲ级的患者,每位患者均采用3D打印多通道施源器和常规阴道单通道施源器进行CT扫描定位,并制定相应计划及评估,运用逆向剂量优化算法计算后得出剂量体积直方图,比较两组施源器用于近距离治疗时的高危临床靶区、膀胱及直肠受照剂量的差异,选择最优计划实施治疗。结果采用3D打印个体化多通道施源设计计划的D_(90)%、D_(100)%、V_(100)%、V_(150)%与常规单通道施源器设计的计划相比,差异均无统计学意义(P>0.05);膀胱、直肠的D2cm3与常规相比均明显降低,差异有统计学意义(均为P<0.05)。结论采用3D打印技术制作的多通道个体化施源器靶区适形性好,可更好地保护膀胱和直肠,有一定的治疗优势。     

Antifungal Activity of Ellagic Acid In Vitro and In Vivo

Ellagic acid (EA) has been shown to have antioxidant, antibacterial, and anti‐inflammatory activities. In Uighur traditional medicine, Euphorbia humifusa Willd is used to treat fungal diseases, and recent studies suggest that it is the EA content which is responsible for its therapeutic effect. However, the effects of EA on antifungal activity have not yet been reported. This study aimed to investigate the inhibitory effect of EA on fungal strains both in vitro and in vivo. The minimal inhibitory concentration (MIC) was determined by the National Committee for Clinical Laboratory Standards (M38‐A and M27‐A2) standard method in vitro. EA had a broad spectrum of antifungal activity, with MICs for all the tested dermatophyte strains between 18.75 and 58.33 µg/ml. EA was also active against two Candida strains, with MICs between 25.0 and 75.0 µg/ml. It was inactive against Candida glabrata. The susceptibility of six species of dermatophytes to EA was comparable with that of the commercial antifungal, fluconazole. The most sensitive filamentous species was Trichophyton rubrum (MIC = 18.75 µg/ml). Studies on the mechanism of action using an HPLC‐based assay and an enzyme linked immunosorbent assay showed that EA inhibited ergosterol biosynthesis and reduced the activity of sterol 14α‐demethylase P450 (CYP51) in the Trichophyton rubrum membrane, respectively. An in vivo test demonstrated that topical administration of EA (4.0 and 8.0 mg/cm²) significantly enhanced the cure rate in a guinea‐pig infection model of Trichophyton rubrum. The results suggest that EA has the potential to be developed as a natural antifungal agent. Copyright © 2015 John Wiley & Sons, Ltd.