全文获取类型
收费全文 | 24421篇 |
免费 | 1981篇 |
国内免费 | 1739篇 |
专业分类
耳鼻咽喉 | 242篇 |
儿科学 | 350篇 |
妇产科学 | 343篇 |
基础医学 | 3187篇 |
口腔科学 | 454篇 |
临床医学 | 3148篇 |
内科学 | 4084篇 |
皮肤病学 | 294篇 |
神经病学 | 1379篇 |
特种医学 | 749篇 |
外国民族医学 | 15篇 |
外科学 | 2698篇 |
综合类 | 3400篇 |
现状与发展 | 6篇 |
一般理论 | 2篇 |
预防医学 | 1530篇 |
眼科学 | 577篇 |
药学 | 2471篇 |
13篇 | |
中国医学 | 1014篇 |
肿瘤学 | 2185篇 |
出版年
2024年 | 57篇 |
2023年 | 320篇 |
2022年 | 777篇 |
2021年 | 1029篇 |
2020年 | 812篇 |
2019年 | 686篇 |
2018年 | 813篇 |
2017年 | 678篇 |
2016年 | 667篇 |
2015年 | 990篇 |
2014年 | 1298篇 |
2013年 | 1070篇 |
2012年 | 1733篇 |
2011年 | 1773篇 |
2010年 | 1047篇 |
2009年 | 875篇 |
2008年 | 1261篇 |
2007年 | 1230篇 |
2006年 | 1239篇 |
2005年 | 1344篇 |
2004年 | 802篇 |
2003年 | 800篇 |
2002年 | 708篇 |
2001年 | 592篇 |
2000年 | 708篇 |
1999年 | 721篇 |
1998年 | 452篇 |
1997年 | 455篇 |
1996年 | 347篇 |
1995年 | 341篇 |
1994年 | 262篇 |
1993年 | 193篇 |
1992年 | 310篇 |
1991年 | 254篇 |
1990年 | 243篇 |
1989年 | 239篇 |
1988年 | 196篇 |
1987年 | 164篇 |
1986年 | 144篇 |
1985年 | 153篇 |
1984年 | 58篇 |
1983年 | 60篇 |
1982年 | 23篇 |
1981年 | 34篇 |
1979年 | 22篇 |
1978年 | 12篇 |
1974年 | 20篇 |
1973年 | 14篇 |
1972年 | 16篇 |
1969年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 296 毫秒
971.
Background and Purpose: During repeat-dose toxicity studies, ECGs are collected from chemically or physically-restrained animals over a short timeframe. This is problematic due to cardiovascular changes caused by manual restraint stress and anesthesia, and limited ECG sampling. These factors confound data interpretation, but may be overcome by using a non-invasive jacket-based ECG collection (JET). The current study investigated whether a jacketed external telemetry system could detect changes in cardiac intervals and heart rate in non-human primates (NHPs), previously implanted with a PCT transmitter.Experimental Approach: Twelve male cynomolgus monkeys were treated weekly with vehicle or sotalol (8, 16, 32 mg kg−1) p.o. ECGs were collected continuously for 24 hours, following treatment, over 4 weeks. A satellite group of six NHPs was used for sotalol toxicokinetics.Key Results: Sotalol attained Cmax values 1–3 hours after dosing, and exhibited dose-proportional exposure. In jacketed NHPs, sotalol dose-dependently increased QT/QTc intervals, prolonged PR interval, and reduced heart rate. Significant QTc prolongation of 27, 54 and 76 msec was detected by JET after 8, 16, and 32 mg kg−1 sotalol, respectively, compared with time-matched vehicle-treated animals. Overall, JET-derived PR, QT, QTc intervals, QRS duration, and heart rate correlated well with those derived from PCT.Conclusions and Implications: The current findings clearly support the use of JET to quantify cardiac interval and rhythm changes, capable of detecting QTc prolongation caused by sotalol. JET may be a preferred method compared to restraint-based ECG because high-density ECG sampling can be collected in unstressed conscious monkeys, over several weeks. 相似文献
972.
973.
Zhangjian Chen Yun Wang Te Ba Yang Li Ji Pu Tian Chen Yanshuang Song Yongen Gu Qin Qian Jinglin Yang Guang Jia 《Toxicology letters》2014
With the extensive application of titanium dioxide (TiO2) nanoparticles (NPs) in food industry, there is a rising debate concerning the possible risk associated with exposure to TiO2 NPs. The purpose of this study is to evaluate the genotoxicity of TiO2 NPs using in vivo and in vitro test systems. In vivo study, the adult male Sprague-Dawley rats were exposed to anatase TiO2 NPs (75 ± 15 nm) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight every day for 30 days. The γ-H2AX assay showed TiO2 NPs could induce DNA double strand breaks in bone marrow cells after oral administration. However, the micronucleus test revealed that the oral-exposed TiO2 NPs did not cause damage to chromosomes or mitotic apparatus observably in rat bone marrow cells. In vitro study, Chinese hamster lung fibroblasts (V79 cells) were exposed to TiO2 NPs at the dose of 0, 5, 10, 20, 50 and 100 μg/mL. Significant decreases in cell viability were detected in all the treated groups after 24 h and 48 h exposure. Significant DNA damage was only observed at the concentration of 100 μg/mL after 24 h treatment using the comet assay. The obvious gene mutation was observed at the concentration of 20 and 100 μg/mL after 2 h treatment using hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutation assay. This study presented a comprehensive genotoxic evaluation of TiO2 NPs, and TiO2 NPs were shown to be genotoxic both in vivo and in vitro tests. The gene mutation and DNA strand breaks seem to be more sensitive genetic endpoints for the detection of TiO2 NPs induced genotoxic effects. 相似文献
974.
Qinghua Dong Sherven Sharma Hai Liu Long Chen Benxing Gu Xiaonan Sun Guanyu Wang 《Toxicology letters》2014
Tumors treated with fractionated doses of ionizing radiation (IR) often acquire radioresistance. Although histone deacetylase inhibitors (HDIs) have been demonstrated to sensitize intrinsic radioresistant cancer cell lines to IR, little is known on the impact of HDIs on the effects of IR in acquired radioresistant cancer cells. This study evaluates the mechanisms by which HDIs sensitize acquired radioresistant esophageal squamous cell carcinoma cells to IR. The HDIs trichostatin A and sodium butyrate were tested for their ability to sensitize acquired radioresistant KYSE-150R and radiosensitive KYSE-150 parental cells to IR. Although the HDIs induced similar levels of cytotoxicity in the KYSE-150 and the KYSE-150R cells, HDIs increased the: (i) radiosensitivity, (ii) IR-induced ROS generation, and (iii) IR-induced G2/M arrest and apoptosis of KYSE-150R cells compared with those of KYSE-150 cells. These changes were accompanied by increased p21expression and decreased mitochondrial membrane potential. When combined with IR, HDIs inhibited Bmi-1 expression in KYSE-150R cells and their ability to repair DNA damage. The results demonstrate the potential utility of HDIs in augmenting the efficacy of fractionated radiotherapy. 相似文献
975.
低促性腺激素性腺功能减退症(hypogonadotropic hypogonadism,HH)是一种罕见的、以低促性腺激素为特征的、引起第二性征及生殖系统发育障碍的疾病。本文将对该疾病定义、病因、临床表现、诊断及治疗进行全面的介绍。由于HH主要影响育龄女性的生育力,因此诱发排卵以及恢复生育力是该疾病治疗中的关键。治疗HH促排卵的方法较正常人特殊而且复杂,本文将着重介绍HH促排卵的各种原理和方法,同时结合最新的国内外进展以及临床实践经验对各种方法的有效性进行探讨。 相似文献
976.
Jinxia Gu Shiyong Du Daowen Han Lujian Hou Jing Yi Ja Xu Guanghui Liu Bin Han Guangwu Yang Zhi-Peng Bai 《Air quality, atmosphere, & health》2014,7(3):251-262
The fine particulate matter samples for 24 h were carried out at the Environment Monitoring Station (EMS) and Shandong Jianzhu University (SJU) sites during 2010 in Jinan City, China. Eight water-soluble ion species were analyzed by ion chromatography, while organic carbon (OC) and elemental carbon (EC) were determined with the IMPROVE thermal optical reflectance method, and 20 inorganic elements were measured by inductively coupled plasma-atomic emission spectrometer and inductively coupled plasma-mass spectroscopy. The annual average mass concentration of PM2.5 was 168.85 μg m?3 at EMS and 148.67 μg m?3 at SJU. The coefficient of divergence was 0.14, 0.19, 0.23, and 0.23 in spring, summer, fall, and winter, respectively, indicating that there was no obvious spatial difference at the two sampling sites. The highest PM2.5, OC, and OC/EC ratio were in winter because of the enhanced emissions from coal combustion for heating and poor atmospheric dispersion. By the method of enrichment factors, the 20 inorganic elements were divided into three types owing to their sources. Al, Si, and Ti were mainly contributed by crustal sources. Na, Mg, P, K, Ca, V, Cr, Mn, Fe, Co, Ni, Ba, and Sr were from both natural emissions and anthropogenic sources. Cu, Zn, Pb, and Sn mainly originated from anthropogenic sources such as vehicular exhaust and industrial emission. Chemical mass closure calculation estimated that SO4 2? was the largest contributor and explained 29.66 % of PM2.5 mass at EMS, while 31.64 % was at SJU. The organic matter, crustal matter, and NO3 –, respectively, accounted for 15.12, 12.87, and 13.77 % to PM2.5 at EMS, while it accounted for 13.46, 13.96, and 14.93 % at SJU, respectively. By the positive matrix factorization analysis, the coal combustion and biomass burning, secondary sulfate, soil dust, secondary nitrate, and vehicle emissions were identified as the major emission sources. 相似文献
977.
Rui Dong Lijing Gu Changhong Guo Feifei Xun Jiali Liu 《Ecotoxicology (London, England)》2014,23(4):674-680
Soil contamination caused by petroleum hydrocarbons has become a worldwide environmental problem. Microorganism combined with phytoremediation appears to be more effective for removal and/or degradation of petroleum hydrocarbons from impacted soils. The current study investigated the effect of inoculated with PGPR Serratia marcescens BC-3 alone or in combination with AMF Glomus intraradices on the phytoremediation of petroleum-contaminated soil. Pot experiments were conducted to analyze the effect on plant and soil for 90 days in greenhouse. The inoculation treatments showed higher plant biomass and antioxidant enzyme activities than the non inoculation control. Inoculation treatments also improved rhizosphere microbial populations in petroleum contaminated soil. The degradation rate of total petroleum hydrocarbons with PGPR and AMP co-inoculation treatment was up to 72.24 %. The results indicated that plant combined with microorganisms for remediation of petroleum hydrocarbons would be a feasible method. 相似文献
978.
979.
980.
蛋白质-能量消耗在慢性肾脏病患者中发病率较高,临床上主要表现为饮食摄入、生化指标、体重指数、肌肉质量等方面的异常,其发病机制非常复杂,跟摄入不足、炎症、代谢性酸中毒、激素水平紊乱、透析相关因素等有关,严重影响透析患者的预后,因此早期发现、诊断和防治显得非常重要,将营养评估列入慢性肾脏病患者诊疗的日常工作当中,并对照诊断标准,及早诊断和采取各种措施防治蛋白质-能量消耗,最终提高慢性肾脏病患者的预后和生存率.本文对慢性肾脏病引起的蛋白质-能量消耗流行病学特点、发病机理、评估方式、治疗及干预措施等进行阐述. 相似文献