KIF23 is a potential biomarker of diffuse large B cell lymphoma: Analysis based on bioinformatics and immunohistochemistry

Diffuse Large B Cell Lymphoma (DLBCL), the most common form of blood cancer. The genetic and clinical heterogeneity of DLBCL poses a major barrier to diagnosis and treatment. Hence, we aim to identify potential biomarkers for DLBCL.Differentially expressed genes were screened between DLBCL and the corresponding normal tissues. Kyoto Encyclopedia of Genes and Genomes and Gene oncology analyses were performed to obtain an insight into these differentially expressed genes. PPI network was constructed to identify hub genes. survival analysis was applied to evaluate the prognostic value of those hub genes. DNA methylation analysis was implemented to explore the epigenetic dysregulation of genes in DLBCL.In this study, Kinesin family member 23 (KIF23) showed higher expression in DLBCL and was identified as a risk factor in DLBCL. The immunohistochemistry experiment further confirmed this finding. Subsequently, the univariate and multivariate analysis indicated that KIF23 might be an independent adverse factor in DLBCL. Upregulation of KIF23 might be a risk factor for the overall survival of patients who received an R-CHOP regimen, in late-stage, whatever with or without extranodal sites. Higher expression of KIF23 also significantly reduced 3, 5, 10-year overall survival. Furthermore, functional enrichment analyses (Kyoto Encyclopedia of Genes and Genomes, Gene oncology, and Gene Set Enrichment Analysis) showed that KIF23 was mainly involved in cell cycle, nuclear division, PI3K/AKT/mTOR, TGF-beta, and Wnt/beta-catenin pathway in DLBCL. Finally, results of DNA methylation analysis indicated that hypomethylation in KIF23''s promoter region might be the result of its higher expression in DLBCL.The findings of this study suggested that KIF23 is a potential biomarker for the diagnosis and prognosis of DLBCL. However, further studies were needed to validate these findings.     

Hand-assisted laparoscopic versus laparoscopic-assisted radical gastrectomy in the treatment of advanced distal gastric cancer: final results of a single-center randomized study

ObjectiveTo compare the results and 5- and 8-year overall survival (OS) and disease-free survival (DFS) of hand-assisted laparoscopic surgery (HALS) and laparoscopic-assisted surgery (LAS) in radical gastrectomy for advanced distal gastric cancer.MethodsA total of 124 patients admitted to our institution from May 2009 to April 2013 were randomly divided into a HALS group (n = 62) and a LAS group (n = 62). Postoperatively, 110 patients were followed for 5 and 8 years, and 14 patients were lost to follow-up. The 5- and 8-year OS and DFS rates of the groups were compared and analyzed.ResultsThe 5- and 8-year OS rates, respectively, were 38.8% and 19.4% in the HALS group and 38.3% and 15.3% in the LAS group (log-rank test, χ² = 0.250). The 5- and 8-year DFS rates, respectively, were 23.1% and 10.6% in the HALS group and 19.3% and 11.6% in the LAS group (log-rank test, χ² = 0.109). No significant differences were found.ConclusionCompared with LAS, HALS radical gastrectomy for advanced distal gastric cancer had a lower conversion rate to open surgery, shorter surgical duration, and more thorough dissection of lymph nodes; 5- and 8-year OS and DFS rates were similar to those with LAS.     

The Potential Roles of Dietary Anthocyanins in Inhibiting Vascular Endothelial Cell Senescence and Preventing Cardiovascular Diseases

Cardiovascular disease (CVD) is a group of diseases affecting the heart and blood vessels and is the leading cause of morbidity and mortality worldwide. Increasingly more evidence has shown that the senescence of vascular endothelial cells is the key to endothelial dysfunction and cardiovascular diseases. Anthocyanin is a type of water-soluble polyphenol pigment and secondary metabolite of plant-based food widely existing in fruits and vegetables. The gut microbiome is involved in the metabolism of anthocyanins and mediates the biological activities of anthocyanins and their metabolites, while anthocyanins also regulate the growth of specific bacteria in the microbiota and promote the proliferation of healthy anaerobic flora. Accumulating studies have shown that anthocyanins have antioxidant, anti-inflammatory, and anti-aging effects. Many animal and in vitro experiments have also proven that anthocyanins have protective effects on cardiovascular-disease-related dysfunction. However, the molecular mechanism of anthocyanin in eliminating aging endothelial cells and preventing cardiovascular diseases is very complex and is not fully understood. In this systematic review, we summarize the metabolism and activities of anthocyanins, as well as their effects on scavenging senescent cells and cardioprotection.     

苯甲酸钠喷雾沸腾造粒

本文介绍一种用流态化技术与喷雾干燥相结合的方法将苯甲酸钠溶液在流化床内完成蒸发、干燥与造粒。     

Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit in vitro

Aim:

To examine the effects of anisomycin on glioma cells and the related mechanisms in vitro.

Methods:

The U251 and U87 human glioblastoma cell lines were tested. The growth of the cells was analyzed using a CCK-8 cell viability assay. Apoptosis was detected using a flow cytometry assay. The expression of proteins and phosphorylated kinases was detected using Western blotting.

Results:

Treatment of U251 and U87 cells with anisomycin (0.01–8 μmol/L) inhibited the cell growth in time- and concentration-dependent manners (the IC₅₀ values at 48 h were 0.233±0.021 and 0.192±0.018 μmol/L, respectively). Anisomycin (4 μmol/L) caused 21.5%±2.2% and 25.3%±3.1% of apoptosis proportion, respectively, in U251 and U87 cells. In the two cell lines, anisomycin (4 μmol/L) activated p38 MAPK and JNK, and inactivated ERK1/2. However, neither the p38 MAPK inhibitor SB203580 (10 μmol/L) nor the JNK inhibitor SP600125 (10 μmol/L) prevented anisomycin-induced cell death. On the other hand, anisomycin (4 μmol/L) reduced the level of PP2A/C subunit (catalytic subunit) in a time-dependent manner in the two cell lines. Treatment of the two cell lines with the PP2A inhibitor okadaic acid (100 nmol/L) caused marked cell death.

Conclusion:

Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit. The regulation of PP2A/C exression by anisomycin provides a clue to further study on its role in glioma therapy.     

盐酸环丙沙星水分的双溶剂费休氏法测定

采用以甲醇为基本溶剂,水为辅助溶剂的双溶剂费休氏法,可准确测定盐酸环丙沙星中分子内和游离状态的全部水分。其平均回收率为１００．０５％,ＲＳＤ为０．２５％,为溶解特性类似的药品提供了可靠的水分测定。     

896例成人嗓音声学参数的计算机采集分析

目的　通过研究获得有关嗓音医学的数据标准及各种嗓音疾病的特征性客观指标 ,建立嗓音临床检测系统 ,为临床诊断与治疗提供有效的参考标准和方法。方法　应用Dr.Speech(DSS)软件的先进技术 ,按卫生部(司 ) :“语音、语言测试和矫治行业标准”的研究要求 ,采集、分析上海地区成人 5 0 0例正常嗓音以及 396例临床常见嗓音疾病的病理性嗓音的声学样本。结果　正常人的各嗓音声学参数 :基频微扰、振幅微扰、声门噪声、频率颤动、共振峰等值均在DSS软件提供的正常范围内 ,而病理性嗓音声学参数超出该范围 ,并具有各不同的特点。结论　我国成人嗓音声学参数与欧美的相似 ,应用Dr.Speech软件可对各种嗓音疾病进行临床客观检测和评估     

甲状腺腺叶切除术精细化操作技术临床结果初步报告

目的 回顾总结集程序化、精细化操作与质控为一体的甲状腺腺叶切除术外科模式在甲状腺全切除术中临床应用结果.方法 2013年5月至2014年8月应用该外科模式实施甲状腺全切除72例,按程序化的设计和步骤进行精细化操作完成手术,对手术切口、手术时间、术中出血量、术中重要结构保护、切口愈合、术后并发症、患者满意度情况进行总结.结果 颈部第二皮纹切口更符合患者立位的社会属性;一侧腺叶手术时间30 ～ 50 min;出血约2～5 ml;无喉上神经损伤,出现暂时性喉返神经麻痹4侧,术后3～4周均自行恢复;暂时性甲状旁腺功能减退10例,术后4～6周自行恢复;切口均一期愈合;手术切口非常满意66例,满意4例,可接受2例.结论 集程序化、精细化操作与质控为一体的甲状腺腺叶切除外科模式对甲状腺手术的实施是一种良好的外科模式,对标准化流程的建立、质控和临床教学均有积极的推动作用.     

PRK术后角膜上皮下雾状混浊的相关因素分析

为探讨准分子激光角膜切削术（ｐｈｏｔｏｒｅｆｒａｃｔｉｖｅｋｅｒａｔｅｃｔｏｍｙ，ＰＲＫ）后角膜上皮下雾状混浊（ｈａｚｅ）的原因及影响因素，对ＰＲＫ后６个月时屈光度在－１．００～－１８．５０Ｄ的３１６例（６１５只眼）近视眼的ｈａｚｅ相关因素进行观察和比较。结果：ＰＲＫ后６个月时ｈａｚｅ发生率为４４．７％，１级或以上ｈａｚｅ为１３．３％。ｈａｚｅ的发生率及密度随屈光度增高而增加（Ｐ＜０．０００１），也随切削深度的增加而增加（Ｐ＜０．０００１），术前非接触眼压超过１８ｍｍＨｇ者，ｈａｚｅ的发生率和密度增加（Ｐ＜０．００５），男性较易发生ｈａｚｅ（Ｐ＝０．０００１），与年龄呈负相关（Ｐ＜０．０５）。而角膜厚度、术前戴用接触镜及散光对ｈａｚｅ的影响不显著。结论：高度近视（＞－６．０Ｄ）和过深的切削（＞８０μｍ）是引起ｈａｚｅ的主要原因。术前眼压偏高、年龄偏低对ｈａｚｅ也有明显影响。对ＰＲＫ治疗高度近视应持审