重组人酸性成纤维细胞生长因子关节腔注射预防骨关节炎软骨退行性变

目的:验证重组人酸性成纤维细胞生长因子关节腔内注射对兔实验性早中期骨关节炎软骨退行性变的防治作用。方法:实验于2005-10/2006-04在暨南大学附属第一医院中心实验室完成。选用健康新西兰大白兔30只,切断膝关节前、后交叉韧带建立兔骨关节炎模型后,按随机数字表法分为3组,空白组、透明质酸钠组和重组人酸性成纤维细胞生长因子组。重组人酸性成纤维细胞生长因子组术后第8周起于手术侧膝关节腔内注射人酸性成纤维细胞生长因子8000IU/(只·周),透明质酸钠组关节腔内注射透明质酸钠1mL/(只·周)。空白组不进行干预。术后第16周空气栓塞法处死动物,大体观察及手术显微镜下观察软骨表面分级;取股骨髁负重面软骨标本进行苏木精-伊红、沙黄染色及骨关节炎组织病理学评分(正常为0～1,重度为10分以上)、分级。结果:30只动物全部进入结果分析。①重组人酸性成纤维细胞生长因子组和透明质酸钠组标本股骨髁负重软骨有轻度粗糙,关节软骨可见浅表溃疡形成。空白组标本股骨髁负重面软骨有轻～中度溃疡形成。②3组动物关节软骨表面分级差异有显著性意义(χ2=22.97,P=0.000)。③3组动物骨关节炎软骨的组织病理学分级差异有显著性意义(χ2=16.84,P=0.005),透明质酸钠组和重组人酸性成纤维细胞生长因子组的骨关节炎组织病理学评分均显著低于空白组(P<0.05)。结论:关节腔注射重组人酸性成纤维细胞生长因子可以较好的延缓兔膝骨关节炎软骨退行性变和预防骨关节炎的发展。     

The role of oxidants and antioxidants in psoriasis

BACKGROUND: Psoriasis vulgaris is a chronic inflammatory skin disease characterized by well-demarcated erythema and scaly plaques. The pathogenesis of psoriasis still remains unclear. An increased reactive oxygen species (ROS) and insufficient antioxidant activity have been determined in psoriatic lesions. AIM OF THE STUDY: To evaluate and compare superoxide dismutase (SOD) and glutathione peroxidase (GP) activity in erythrocytes, catalase (CAT) activityand malondialdehyde (MDA) levels in serum of subjects with psoriasis and controls as well as MDA levels in skin biopsies from both groups. STUDY POPULATION: Twenty-two psoriatic patients (12 women and ten men) and 22 (12 women and ten men) healthy controls were involved in this study. FINDINGS: Statistically significant decreased levels of erythrocyte SOD and GP activities were noted in psoriatic subjects. Furthermore, a statistically significant increased serum CAT activity was found in the psoriasis group. No statistically significant difference was found in the serum MDA levels in the two groups, however, statistically significant increased tissue levels of MDA were noted in the psoriasis group. CONCLUSIONS: Our results support the hypothesis of an imbalance in the oxidant-antioxidant system in psoriasis.     

A case of sarcomatoid carcinoma of the skin

We describe the histological and immunocytochemical findings of an exophytic cutaneous tumour with mixed features of atypical fibroxanthoma (AFX) and basal cell carcinoma (BCC). A 73-year-old woman presented with a rapidly growing tumour measuring 35 mm in diameter and 10 mm in height on the left forearm. The tumour was excised and histology revealed a biphasic tumour with a pleomorphic spindle cell component and an associated tumour composed of discrete islands of atypical basaloid cells with peripheral palisading consistent with BCC. The two tumours merged into each other at one point. The spindle cell tumour showed a positive immunocytochemical reaction to fibrohistiocytic marker of KP-1 (CD68) and a negative immunocytochemical reaction to AE1/AE3, CAM5.2, S-100 and HMB-45, features consistent with AFX. Immunocytochemistry of the basaloid tumour showed a positive reaction to epithelial markers AE1/AE3 and CAM5.2, and a negative reaction to S-100, HMB-45 and KP-1 (CD68). To date, 15 cases of primary cutaneous carcinosarcoma have been reported in the literature. It has been postulated that these tumours may originate from undifferentiated progenitor cells capable of producing multiple cell lines.     

中药女贞子中水溶性成分二种新裂环环烯醚萜甙的分离和鉴定

从木犀科植物女贞(Ligustrum lucidum Ait)干燥成熟果实女贞子中分离得到2个新的裂环环烯醚萜甙类化合物,经光谱及化学分析,鉴定其化学结构,并分别命名为特女贞甙(specnuezhenide,I)和女贞苦甙(nuezhengalaside,I)。     

Excess Lifetime Cancer Risk Associated with Granite Bearing Radioactive Minerals and Valuable Metals,Monqul Area,North Eastern Desert,Egypt

The present work is concerned with assessing the cancer risk contributed by the studied granite types including valuable metals, such as Cu, Au, and Ba mineralization, as well as radioactive-bearing mineralization, such as monazite and zircon, in south Monqul at Wadi Makhrag El Ebel, north Eastern Desert, Egypt. The mineralization analyses illustrated that copper mineralization containing chrysocolla and tenorite minerals were restricted to the alteration zone, especially (argillic, phyllic, and propylitic) in monzogranite. However, barite veinlets had an ENE–WSW trend, while gold mineralization was confined to quartz veins having NE–SW trends. Monazite and zircon are radioactive-bearing minerals recorded in monzogranite causing high radioactive zones in south Monqul. The radionuclide activity concentrations were detected in the studied monzogranites. The mean values of A_U (103 ± 91 Bq kg⁻¹), A_Th (78 ± 19 Bq kg⁻¹), and A_K (1484 ± 334 Bq kg⁻¹) in the monzogranite samples were higher than the recommended worldwide average. The change in radioactive-transporting minerals found inside granite faults caused the high amounts of radioactivity seen in the samples. Due to the monzogranites being applied in building materials, the radiological hazards were assessed by calculating risk indices such as annual effective dose (AED) and excess lifetime cancer risk (ELCR). The acceptable limit for the ELCR readings was surpassed. As a result, the investigated monzogranite samples are not suitable for use in infrastructure materials.