海博刀与三角刀在经口内镜下肌切开术中疗效的回顾性队列研究

目的 比较海博刀与三角刀在经口内镜下肌切开术(POEM)中的临床疗效。方法 采用回顾性队列研究设计，纳入2012年6月至2014年7月因贲门失弛缓症在南方医科大学南方医院接受POEM治疗的患者，术中使用海博刀者为海博刀组，使用注射针和三角刀者为三角刀组，比较两组手术相关参数、术后症状缓解及并发症发生率。结果 共纳入57例患者，其中海博刀组25例，三角刀组32例。两组患者基线特征比较差异无统计学意义（P>0.05）。海博刀组平均手术时间短于三角刀组[(55.3±17.7)min比(69.5±9.4)min，P=0.038)]；术中平均器械交换次数少于三角刀组[(4.5±1.5)次比(10.7±1.7)次，P=0.000]。所有患者无严重不良事件发生。在1年的随访中，海博刀组治疗成功率92.0%(23/25)，三角刀组96.9%(31/32)，差异无统计学意义（P=0.576)。结论 海博刀能显著缩短POEM手术时间，并且获得与三角刀相似的治疗成功率。     

光疗治疗银屑病的研究进展

目前治疗银屑病的方法较多,但治疗效果令人满意的方法尚少。光疗是治疗银屑病的一种有效方法,与药物联合应用不仅可以提高疗效,还可减少不良反应的发生。现将近年光疗治疗银屑病的研究进展综述如下。     

Notch-1在木村病中的表达及意义

目的探讨Notch-1在木村病的表达,指导木村病患者的优化治疗。方法通过免疫组化SP方法评估Notch-1蛋白在40例木村病患者组织和15例慢性淋巴结炎组织中的表达,分析Notch-1与木村病临床病理资料及预后的关系。结果 40例木村病患者中,男性患者占92.5%(37/40),7.5%(3/40)为女性患者,发病平均年龄为38岁。肿块的直径范围为1.0⁶.5 cm。Notch-1在复发的11例木村病患者中,63.6%(7/11)高度表达,36.4%(4/11)低度或中度表达。Notch-1表达、嗜酸粒细胞计数、肿块最大直径与复发有统计学差异(P<0.05),年龄、性别、肿块部位、肿块个数与复发无相关性(P>0.05)。结论 Notch-1高表达可能为木村病复发患者的一个有效观察指标,可为预后提供评估途径及指导患者个体优化治疗。     

Clinical outcomes after percutaneous coronary intervention for early versus late and very late stent thrombosis: a systematic review and meta-analysis

Journal of Thrombosis and Thrombolysis - Whether the clinical outcomes of stent thrombosis (ST) are different when stratified by time of occurrence remains unclear. The objective of this study was...     

羊水间充质干细胞体外培养方案的优化及生物学特性

背景：目前干细胞工程种子来源多样化,羊水中存在胎儿脱落细胞,可分离培养出间充质来源干细胞。目的：探索不同成分培养基对人羊水间充质干细胞（human amniotic fluid-derived mesenchymal stem cells,h-AFMSCs）体外培养的影响,并分析最适培养基体外培养的h-AFMSCs的生物学特性,建立优化的h-AFMSCs培养方案。方法：孕16～24周的孕妇产前检查中获得羊水进行原代及传代培养并采用6种不同的培养基成分对h-AFMSC进行培养。结果与结论：从孕中期羊水中可分离出h-AFMSCs,体外使用低糖DMEM培养基（L-DMEM）＋体积分数10%胎牛血清与合成培养基MESEN PRO体积比1：1配制的培养基对其扩增促进作用最强。h-AFMSCs高表达CD29、CD73、CD90、CD105、CD166,低表达CD14、CD34、CD45及HLA-DR;分离培养的h-AFMSCs高表达的干细胞基因为OCT-4和Nanog;h-AFMSCs体外具有向成骨、成脂细胞分化的潜能且具有抑制淋巴细胞增殖的作用。提示孕中期羊水是低免疫原性的h-AFMSCs的良好细胞来源。     

临床护士共享治理在给药安全管理中的实践

目的 将共享治理的管理理念运用到给药安全管理中,提高护士给药安全的风险管理意识和参与度,降低给药错误隐患事件和给药错误发生率.方法 2019年7～12月将共享治理应用于病房给药安全管理中,并与2019年1～6月未实施共享治理时的给药隐患事件、给药错误发生率、护理人员决策参与程度等进行对比.结果 实施共享治理后给药隐患事件、给药错误发生率显著低于实施前(P＜0.05,P＜0.01);实施共享治理后护理人员决策参与度和给药安全理念得分显著高于实施前(均P＜0.01).结论 共享治理能有效提高护士给药安全的风险管理意识,降低给药错误及隐患事件的发生.     

Professional identity and nursing: contemporary theoretical developments and future research challenges

JOHNSON M., COWIN L.S., WILSON I. & YOUNG H. (2012) Professional identity and nursing: contemporary theoretical developments and future research challenges. International Nursing Review Aim: We propose that the conceptual orientation of professional identity is a logical consequence of self‐concept development by focusing on career and its meaning and presents a measurable set of concepts that can be manipulated to improve retention of student and registered nurses within health service. Background: Although professional identity is a term that is commonly written of in nursing literature, its theoretical origins remain unclear, and available empirical evidence of its presence or ability to change is omitted from nursing research. Sources of evidence: We present a professional identity pathway and explore the factors that influence professional identity throughout a career in nursing. Discussion: Nurses' professional identities develop throughout their lifetimes, from before entering nursing education, throughout their years of study and clinical experience, and continue to evolve during their careers. Education is, however, a key period as it is during this time students gain the knowledge and skills that separate nurses as professional healthcare workers from lay people. Conclusion: Finally, a call for longitudinal studies of students to graduates, using conceptually derived and psychometrically proven instruments capable of detecting the subtle changes in the construct over time, is recommended. Further empirical research into the theoretical concepts that underline professional identity, and the factors that influence changes in this important construct in nursing, is required. Ultimately, the practical relevance of such research will lie in the potential it provides for enhanced nursing career support and improved workforce policies.     

白酒火浴法治疗肌注后局部肿痛治验举隅

臀部肌肉注射是临床上最为常见的给药途径之一,由于药物的性质、患者体质的差异或临床操作不当等原因,部分患者会出现注射部位局部肿、痛、结节等并发症,异常痛苦,甚至影响药物疗效的发挥。国术点穴疗法是由拳术演变来的一种自然疗法,具有方法简便、容易操作、花费少、疗效好等优点。临床中我们用国术点穴疗法中的白酒火浴法治疗臀部肌注后局部肿痛,无不效验。     

股骨干骨折合并胸部损伤患者326例观察

目的探讨股骨干骨折合并胸部损伤患者的疗效方式。方法回顾分析1998年5月至2010年10月该院收治的326例股骨骨折合并胸部损伤患者的临床资料。结果术中、术后患者均未出现脂肪栓塞综合征(FES),2例病情不稳定患者外固定架固定后出现急性呼吸窘迫综合征(ARDS)。结论股骨干骨折合并胸部损伤患者应根据实际情况选择不同的术式和手术时机。