三仙汤对成骨细胞增殖的影响

目的通过对三仙汤促进成骨细胞株增殖的研究,判断三仙汤能否应用于绝经后骨质疏松症的防治。方法应用UMR-106成骨细胞株,采用体外细胞培养技术,以氟化钠为阳性对照药,用MTT法测定三仙汤对成骨细胞增殖的影响。结果氟化钠1.25μmol/L、5μmol/L组与空白组相比有显著性差异(前者P<0.01,后者P<0.001);10μmol/L组与空白组无明显差别。三仙液在5、10、100、500、1000mg/L5个不同浓度均使MTT法检测的光吸收值显著升高(P<0.001或P<0.01)。结论三仙汤能促进体外培养成骨细胞增殖。     

急性肺血栓栓塞症猪血流动力学及心肌损伤的研究

目的　观察急性肺血栓栓塞症 (PTE)猪血流动力学、动静脉血气及心肌肌钙蛋白Ⅰ(cTnⅠ )、肌红蛋白 (Mb)、肌酸激酶同工酶 (CK MB)水平。方法　健康幼年猪 16只 ,分为 2组 ,每组8只。栓塞组 (急性PTE模型组 ) :用注射器经颈外静脉插管快速 1次注入多聚乙烯微球 ,0 1g/kg体重悬浮于生理盐水 5 0ml中 ;对照组 :用注射器经颈外静脉插管快速 1次注入生理盐水 5 0ml。检测栓塞前、栓塞后即刻、30min、1h、2h、3h血流动力学及血气的变化 ,同时检测血清cTnⅠ、Mb、CK MB水平。结果　栓塞即刻肺动脉压升高 ,约为栓塞前的 2～ 3倍 ,栓塞后 2～ 3h逐渐恢复至栓塞前水平 ;心输出量、肺毛细血管嵌顿压、肺动脉压收缩压、舒张压无明显变化。栓塞即刻动脉血氧分压(PaO2 )下降 ,动脉血二氧化碳分压升高 ,pH值下降 ,30～ 6 0min达最高峰 ,2h后PaO2 和 pH值恢复至栓塞前水平。栓塞后血清cTnⅠ和Mb明显升高 ,CK Mb栓塞前后无明显变化。cTnⅠ与Mb有相关性 (r =0 5 5 ,P =0 0 0 1) ,与CK MB无相关性 (r =0 10 9,P =0 5 33)。结论　急性PTE可出现急性肺动脉压升高和气体交换障碍等病理生理改变。检测cTnⅠ和Mb有助于急性PTE心肌损伤早期诊断     

F gene recombination between genotype II and VII Newcastle disease virus

A velogenic Newcastle disease virus (NDV) strain, designated as SRZ03, was isolated from an egg layer flock with NDV vaccine immunization failure in China in 2003. Recombination was found in the F gene of SRZ03. Complete genome sequences analysis indicated that the N-terminal of SRZ03 F gene originated from a genotype II NDV strain, whereas the C-terminal of F gene and the rest of the genes originated from a prevalent velogenic genotype VII NDV strain. It provides us valuable information for understanding the recombination of nonsegmented negative-sense RNA viruses.     

聚醚砜表面光固定脲酶的研究

我们利用含芳香叠氮基的光活性酯将脲酶光固定在聚醚砚（Polyether sulfone,PES）膜的表面。同时研究了紫外光辐照时间对固定化脲酶密度、固定化脲酶活性的影响，温度、PH值对自由脲酶、固定化脲酶相对活性的影响；并考察了固定化脲酶的相对活性随光活性酶浓度的变化、固定化脲酶的重复使用次数及储存稳定性等性质。结果表明，PES膜表面固定化脲酶的浓度为0.33mg/cm^2；当紫外光辐照时间为5分钟时，固定化脲酶的相对活性最高；固定化脲酶的最适Ph值和最适温度分别为7℃和50 ℃；在50℃时，连续使用12次后，固定化脲酶仍具有50%的催化活性；在4℃保存储存一个多月仍可保持80%的催化活性。     

基于敏感性定理的EIT重构算法仿真研究

为了研究性能更好的EIT图像重构算法，我们对基于敏感性定理的EIT图像重构算法进行了计算机仿真研究，并与几种常用图像重构算法进行比较，提出对基于敏感性定理的EIT图像重构算法的改进措施。     

Evaluation of S100A4 mRNA in EUS-FNA specimens for the assessment of chemosensitivity to gemcitabine from patients with unresectable pancreatic cancer

Background/Aims: Gemcitabine (GEM) is the first-line chemotherapy in patients with unresectable pancreatic cancer. However, the clinical outcomes of this regimen are still unsatisfactory in prolonging survival. Resistant to GEM is one of the reasons for poor prognosis. Therefore, looking for molecular biomarkers to predict chemosensitivity to GEM is important for treatment in unresectable pancreatic cancer patients. The aim of this study was to analyze S100A4 mRNA in tissues of unresectable pancreatic cancer obtained by endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNA), and to determine the relation between S100A4 mRNA level and chemosensitivity to GEM. Methods: The analysis was performed on samples from 36 patients with unresectable pancreatic cancer who were treated with gemcitabine alone. The patients were assigned to receive GEM at 1,000 mg/m²/wk for weeks 1 to 6, followed by 1 week rest, then for 4 weeks. mRNA was extracted for S100A4 mRNA assay from patients above by EUS-FNA before GEM-treatment. The 36 patients were divided into the following two groups. Patients with partial response and those with stable disease whose tumor markers decreased by 50% or more were classified as the effective group. The rest of patients were classified as the non effective group. The relationship between GEM efficacy and S100A4 mRNA expression was then examined by chi-squared test. Results: S100A4 mRNA showed a significant correlation with GEM efficacy. Patients in the effective group had low S100A4 mRNA expression, whereas patients in non-effective group had high S100A4 mRNA expressions (P = 0.0059). Conclusion: S100A4 mRNA level analyzed in EUS-FNA samples is an important molecular biomarker for prediction of chemosensitivity to GEM in unresectable pancreatic cancer.     

普米克令舒治疗毛细支气管炎的研究进展

本文回顾了普米克令舒在临床上治疗小儿毛细支气管炎的应用。对在不同实验中使用普米克令舒的疗效进行分析。证实普米可令舒在治疗毛细支气管炎时,疗效显著。提示普米克令舒对毛细支气管炎的治疗起较重要的作用。     

影响女性失业者心理健康的因素和作用机制

目的考察影响女性失业者心理健康的因素和作用机制。方法采用问卷法对416名唐山市50岁以下的失业女职工进行测量。结果1失业时间长短对不同的应对方式均无显著预测作用,但对心理健康有直接影响;2目前是否已再就业对心理健康无显著预测作用,也不能通过不同的应对方式间接影响心理健康;3社会支持、成熟型应对方式可以显著预测心理健康,而不成熟型和合理化的应对方式对心理健康无显著预测;4不同应对方式在社会支持和心理健康之间起部分中介作用。结论失业时间越长,心理健康状况越好;社会支持越高,成熟型应对方式使用越多,心理健康水平越高;不同应对方式在社会支持和心理健康之间起着重要的中介作用,社会支持越高,越有利于增加成熟型应对方式的使用,减少不成熟型和合理化应对方式的使用,从而更好地保护心理健康,免受应急伤害。     

通过多种技术方法的综合使用优化全脑全脊髓照射技术

全脑全脊髓照射是针对多种癌症的治疗流程中一个重要的组成部分。为了达到最佳的肿瘤控制,需要在靶区体积的确定、重要正常组织的保护、剂量均匀度、射野交接区域以及剂量测定方面都特别注意。本文提出了一种适用于大多数治疗情况的优化了的全脑全脊髓照射治疗技术,以一名因生殖细胞瘤需接受全脑全脊髓照射治疗的17岁男性患者为例,通过综合使用半野衔接技术、扩展半影的射野衔接技术和多叶光拦子野技术,制定临床可实行的治疗计划,大大改善了脊髓靶区内的剂量均匀度,将剂量最大点由处方剂量的124%降低到处方剂量的108%。对仿真人体模进行的实际测量值与计算值的比较表明,测量值和计算值是基本一致的。