何首乌组织培养的研究

采用单因子、正交设计法考察了基本培养基、激素以及光暗条件对何首乌幼嫩茎、叶诱导愈伤组织的影响,以及培养时间与愈伤组织生长量间的关系。实验研究表明:MS 2,4D1mg/L 6-BA1mg/L IBA0.5mg/L和暗培养对诱导何首乌愈伤组织产生效果较好;接种12天时愈伤组织生长量达到最大;愈伤组织经诱导能够产生完整植株,成苗率为95％。     

大鼠脑神经干细胞神经巢蛋白表达的流式细胞术检测

检测胎鼠及成年大鼠脑内神经干细胞特异性抗原神经巢蛋白的表达,判明啮齿类哺乳动物脑内神经干细胞存在的可能性及相应的部位。方法：显微手术下采取胎鼠及成年大鼠大脑与皮质下脑组织,间接荧光法标记ｎｅｓｔｉｎ,以流式细胞术检测。结果胎鼠脑内普遍存在ｎｅｓｔｉｎ阳性细胞,成年大鼠脑室旁组织中也存在ｎｅｓｔｉｎ阳性细胞。     

实验性脑损伤后脑组织内腺苷、肌苷含量的变化及其意义

研究大鼠急性脑损伤后腺苷含量变化及其生代代谢。方法：采用大鼠液压脑损伤模型,用高效液相色谱法测定大鼠脑损伤前后脑皮质内腺苷和肌苷含量。结果：大鼠脑损伤后１０ｍｉｎ,伤侧大脑皮质内腺苷含量即明显升高,与对伤后３０ｍｉｎ升至最高值,伤后２ｈ腺苷含量恢复正常水平。结论腺苷参与继发性脑损伤后的病理生理过程。     

嗓音频谱分析中／a／,／i／音采样的比较

应用486微型计算机和北京邮电大学通用信号谱分析系统分别对30例青年女性喉病患者和36例健康对照组的/a/、/i/音嗓音信号进行频谱分析,比较其相对信噪比,结论是在频谱分析中用/a/音采样较/i/音更易检出病态嗓音.     

A longitudinal,functional study of peripheral nerve recovery in the mouse

Objectives: To verify the validity of the recently described sciatic functional index for mice to monitor neuronal functional recovery over time using a blinded, randomized, and controlled evaluation. Study Design: Surgery was performed on the left sciatic nerves of 62 C57/BL mice after randomly assigning them to one of four surgical groups: sham surgery, sciatic nerve crush, nerve transection without repair, and nerve transection followed by epineurial suture repair. Sciatic functional indices were measured before surgery and then after surgery at 10-day intervals for 90 days, using a previously described formula. Results: Sham surgery did not affect nerve function when compared with preoperative values (P > .24). Crush surgery produced a reversible nerve injury that fully recovered after 20 days. Nerve transection without repair resulted in complete functional disability without recovery of function during the 90-day follow-up interval. When transected nerves were repaired, complete functional disability was noted at day 10, with subsequent functional recovery to 26% of function at day 30. This level of recovery persisted until the 60th postoperative day when muscle contractures resulted in progressive worsening of the index. There were statistically significant differences between the sciatic functional indices of each of the groups (P < .05). Conclusions: The previously described sciatic functional index for mice is an accurate indicator of the level of sciatic neuronal function during recovery. This index represents a method of evaluating neuronal function that may provide a better reflection of the recovery parameters that are important in clinical situations. The sciatic functional index will allow for study of sciatic nerve functional recovery in genetically engineered transgenic mice.     

Toxic cardiogenic shock in a patient receiving weekly 24-h infusion of high-dose 5-fluorouracil and leucovorin

A 54-year-old man was treated with weekly 24-h infusion of high-dose 5-fluorouracil (2600 mg/m2) and leucovorin (100 mg/m2) for metastatic colon cancer. At first, he tolerated the treatment well and no significant toxicity was identified. After a total of eight courses of treatment, a stable disease was observed, but mild shortness of breath was found on occasion. The patient had no previous history of cardiac disease and the heart performance assessed by left ventricular ejection fraction before treatment was normal. Unfortunately, acute pulmonary edema with lethal cardiogenic shock occurred during the ninth course of treatment, in spite of intensive medical treatment. The chest X-ray showed extreme cardiomegaly. Repeated assessment of his heart function by echocardiogram and ventricular ejection fraction revealed a very poor cardiac performance. Toxic cardiogenic shock during weekly 24-h infusion of high-dose 5-fluorouracil and leucovorin is extremely rare. To the best of our knowledge, no case has been reported in the English literature. We report a case and the relevant literature about the incidence, clinical picture and possible pathophysiology on 5-fluorouracil-related cardioxicity is reviewed.      

乳腺癌肿瘤血管生成的临床病理学意义

肿瘤血管生成（ＴｕｍｏｒＡｎｇｉｏｇｅｎｅｓｉｓ，ＴＡ）是目前肿瘤研究的重要课题，其研究目的在于探讨ＴＡ的预后价值以及预测肿瘤对抗血管生成药物的反应。本文应用第八因子相关抗原多克隆抗体对６５例乳腺浸润性导管癌（以下简称乳腺癌）进行了微血管的定量研究。结果显示：腋下淋巴结阳性病例组织的微血管密度（ＭｉｃｒｏｖｅｓｓｅｌＤｅｎｓｉｔｙ，ＭＶＤ）（１２９．７±４４．９）明显高于腋下淋巴结阴性（ＮｏｄｅＮｅｇａｔｉｖｅＢｒｅａｓｔＣａｎｃｅｒ，ＮＮＢＣ）病例组的ＭＶＤ（７９．６±３３．６），差异呈极显著性（Ｐ＜０．００１）；发生术后复发及远处转移的病例的ＭＶＤ均值高达１４５．３；以上结果提示乳腺癌ＭＶＤ与肿瘤转移、复发均密切相关。我们认为乳腺癌ＭＶＤ可反映其血供状态，ＭＶＤ高的病例微血管丰富，肿瘤组织生长快，癌细胞易于进入微循环而发生转移     

增强PHA-LAK细胞诱导激活新途径的研究

自1995年在上海召开第四届全国肿瘤生物治疗会议以来,我国的肿瘤生物治疗研究和应用发展迅速并取得了新的进展;与此同时,国外又涌现出许多新的理论和方法.因此,召开一次有关的全国性专业学术会议将对我国肿瘤生物治疗的深入研究及临床应用乃至未来的发展具有重要意义.由中国免疫学会肿瘤免疫与生物治疗专业委员会、中国抗癌协会肿瘤生物治疗专业研究会及中华医学会肿瘤学会联合主办的“第五届全国肿瘤生物治疗会议”于1997年9月21～25日在四川成都召开.这次会议共收到稿件429篇,为历届肿瘤生物治疗会议之最,也表明目前国内肿瘤生物治疗研究之兴盛.其中,21篇作了大会发言;203篇以摘要形式在《中国肿瘤生物治疗杂志》上发表;120篇参加了分组讨论.这次会议的主要议题是:(1)总结和交流自第四次全国肿瘤生物治疗会议以来我国肿瘤生物治疗领域的基础研究及临床工作,对此作出恰当的评价并增进对国外最新进展的了解;(2)介绍各单位在肿瘤生物治疗领域的研究及临床工作,从而加强国内同行彼此之间的交流与合作;(3)学习肿瘤生物治疗的规范化要求、新药技术的审评要求以加强对肿瘤生物治疗研究及应用的管理;(4)通过了解与学习国外该领域的最新进展,根据我国的国情以及研究基础提出国内肿瘤生物治疗领域今后的发展     

ODC反义寡核苷酸对K562细胞生长抑制作用

鸟氨酸脱羧酶(ornithine decarboxylase,ODC)是催化多胺生物合成的限速酶.过去的研究表明多胺在维持肿瘤恶性表型中起着重要作用.用ODC不可逆抑制剂抑制多胺生物合成不仅能抑制肿瘤细胞的增殖而且还诱导细胞分化.近年我们采用反义技术,以ODC反义RNA转染肿瘤细胞,通过对ODC表达的封闭,成功地使其恶性表型得到了逆转.提示抑制肿瘤细胞的多胺合成可能是抗癌的有效途径.本文用ODC反义寡核苷酸控制肿瘤细胞的多胺生物合成,观察了其对K562细胞生长特性的影响,以期为ODC反义寡核苷酸在肿瘤临床的应用提供实验依据.     

喉恶性淋巴瘤12例报道

目的回顾性分析了本院自１９７８～１９９５年收治的１２例Ⅰ～Ⅱ期喉恶性淋巴瘤的治疗情况。方法全部病例均予局部放射治疗和全身化疗，放疗剂量４５～７２Ｇｙ（中位５７Ｇｙ），联合化疗方案包括ＣＯＰ、ＣＯＦ、ＣＨＯＰ（１～６疗程）。结果一、三、五年生存率分别为８３．３％、５７．１％、４５．７％（寿命表法）。２例治疗后半年内死于胃肠道淋巴瘤，１例治疗后５年出现扁桃体淋巴瘤。结论放疗和化疗的联合治疗对Ⅰ～Ⅱ期喉恶性淋巴瘤的预后较好。