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101.
A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin 下载免费PDF全文
The cytolethal distending toxin (CDT) of Haemophilus ducreyi is comprised of the CdtA, CdtB, and CdtC proteins, with the CdtB protein having demonstrated enzymatic (i.e., DNase) activity. Using a single recombinant Escherichia coli strain with two plasmids individually containing the H. ducreyi cdtA and cdtC genes, we purified a noncovalent CdtA-CdtC complex. Incubation of this CdtA-CdtC complex with HeLa cells blocked killing of these cells by CDT holotoxin. Furthermore, the addition of purified recombinant CdtB to HeLa cells preincubated with the CdtA-CdtC complex resulted in the killing of these human epithelial cells. 相似文献
102.
应用细胞原位杂交技术,观察经重组小鼠白细胞介素-19(IL-1β)处理后的体外培养的新生1d大鼠中脑黑质神经元c-jun基因的表达.结果显示,培养的黑质细胞多为酪氨酸羟化酶阳性神经元,IL-1β可诱导体外培养的黑质神经元c-junmRNA表达,高水平的表达出现在IL-1β处理后2~4h。说明IL-1β有兴奋黑质神经元的作用,并提示黑质神经元上可能存在IL-1β受体. 相似文献
103.
Peng‐Yuan Liu Yue‐Juan Qin Robert R. Recker Hong‐Wen Deng 《American journal of human biology》2004,16(1):68-77
Osteoporosis is a major public health problem defined as a loss of bone strength, of which bone size is an important determinant. In the present study, familial correlation and segregation analyses for the spine and hip bone sizes were performed for the first time in a Chinese sample composed of 393 nuclear families with a total of 1,193 individuals. The results indicate a major gene of codominant inheritance for spine bone size; however, there is no evidence of a major gene influencing hip bone size. Significant familial residual effects are found for both traits, suggesting their polygenic inheritance. Heritability estimates (±SE) for spine and hip bone size were 0.62 (0.13) and 0.59 (0.12), respectively. Sex and age differences in genotype‐specific average bone size were observed. Compared with our previous study on bone mineral density (BMD) in the same population, this study suggests that genetic determination of bone size may be different from that of BMD, and thus studying bone size as one surrogate phenotype for osteoporotic fractures may be necessary. Am. J. Hum. Biol. 16:68–77, 2004. © 2003 Wiley‐Liss, Inc. 相似文献
104.
目的:探讨血小板源性生长因子(PDGF)在低氧肺动脉高压血管构形重建发生中的作用。方法:应用免疫组化技术结合计算机图像分析,检测了低氧大鼠腺泡内肺动脉(IAPA)PDGF-B链蛋白表达水平。结果:常氧时,IAPA仅有PDGF-B链蛋白弱表达;低氧1天时,IAPA便有较强的PDGF-B链蛋白表达,定位于IAPA的内皮和中膜,低氧3天至14天仅分布于中膜;低氧1、3、5、7、14天各时间点PDGF-B链蛋白表达分别为常氧组的1.53、1.59、1.56、1.62和1.42倍,差异有显著性(P<0.01)。结论:PDGF-B链蛋白可能参与了低氧肺动脉高压血管构形重建的发病过程 相似文献
105.
人胚胎肺内神经内分泌细胞电镜观察 总被引:1,自引:0,他引:1
用透射电镜对不同胎龄的人胚胎肺内神经内分泌细胞进行了发生和超微结构观察。肺内支气管上皮内未分化细胞,第八周开始向神经内分泌细胞转化,提示神经内分泌细胞对早期胚胎肺的发生、发育有特殊意义。神经内分泌细胞内致密核心小泡(DCV)及其它与内分泌活动有关的各种细胞器发达。能见到P0细胞、P1细胞、P2细胞及P3细胞等四种类型的神经内分泌细胞。P0细胞的发生及分化程度表明它可能是一种前细胞,它可以进一步转化 相似文献
106.
Recombinant antibody cloning and phage display technologies were used to produce single-chain antibodies (scFv) against Clostridium difficile toxin B. The starting material was the mouse B cell hybridoma line 5A8, which generates a monoclonal antibody against the toxin. The integrated cloning, screening, and phage display system of Krebber et al. (J. Immunol. Methods 201:35-55, 1997) allowed us to rapidly obtain toxin B-binding scFv sequences derived from the hybridoma cell line. The best candidate scFv sequences, based on preliminary enzyme-linked immunosorbent assay (ELISA) screening data were then subcloned into the compatible expression vector. Recombinant single-chain antibodies were expressed in Escherichia coli. A 29-kDa band was observed on polyacrylamide gel electrophoresis as predicted. The expressed product was characterized by immunoblotting and detection with an anti-FLAG antibody. The toxin B-binding function of the single-chain antibody was shown by a sandwich ELISA. The antibody was highly specific for toxin B and did not cross-react with material isolated from a toxin B-negative C. difficile strain. The sensitivity of the soluble single-chain antibody is significantly higher than the original monoclonal antibody based on ELISA data and could detect a minimum of 10 ng of toxin B/well. Competitive ELISAs established that the affinity of the 5A8 parent antibody and the best representative (clone 10) of the single-chain antibodies were similar and in the range of 10(-8) M. We propose that recombinant antibody technology is a rapid and effective approach to the development of the next generation of immunodiagnostic reagents. 相似文献
107.
108.
109.
Ueno T Tremblay J Kunes J Zicha J Dobesova Z Pausova Z Deng AY Sun YL Jacob HJ Hamet P 《Journal of molecular medicine (Berlin, Germany)》2003,81(1):51-60
Acute pharmacogenetic analysis was carried out in an intercross F2 population derived from Prague hypertensive-hypertriglyceridemic and Lewis rats. Quantitative trait loci (QTL) mapping was performed for baseline blood pressure (BP) and for BP after blockade of the renin-angiotensin system by losartan, of the sympathetic nervous system (SNS) by pentolinium, and of the nitric oxide system by N(G)-nitro- L-arginine methyl ester. Two significant loci for baseline BP were found on chromosome (Chr) 3 (logarithm of likelihood, LOD, 3.8) and Chr 5 (LOD 3.6), and one suggestive locus on Chr 1 (LOD 2.7). The QTL on Chr 3 persisted after treatment with the three agents while the QTL on Chr 5 and Chr 1 disappeared after pentolinium administration. This suggests independence of the locus on Chr 3 from each acute BP regulatory system examined, whereas the loci on Chr 5 and Chr 1 appeared to be controlled mainly by the SNS. Although not apparent at baseline, a significant locus appeared on Chr 8 (LOD 7.0) after blockade of the SNS, and NO system blockade led to the appearance of a new QTL on Chr 1 (LOD 3.6), indicating the contribution of the inhibited systems to these loci. Pharmacogenetic dissection of the BP trait is a powerful tool to unravel the underlying physiological mechanisms of QTL affecting baseline BP and to identify specific QTL for the response to drugs. This pharmocogenetic approach enabled us to determine the main causative acute BP regulatory systems and should lead to better selection of suitable antihypertensive drugs for individual patients. 相似文献
110.
评价新型的双极和三极导管自动心室除颤系统电除颤对左心室收缩和舒张功能的影响。动物麻醉后,在X光机指导下,分别在10只犬心脏内装置双极导管自动除颤系统(组Ⅰ);在10只猪心脏内装置三极导管自动除颤系统(组Ⅱ);并行电除颤试验。使用食管超声心动图在电除颤前后记录二维、M型和多谱勒超声图像。组I动物接受4次电除颤,电量为64J;组Ⅱ接受平均8次电除颤,电量为210J。结果显示:左室收缩面积分数、左室等容舒张时间和二尖瓣血流E波与A波速度比值以及时间-流速积分比值等反映左室舒缩功能的指标在两组动物除颤后均无显著改变。研究表明:两种经静脉导管自动心室除颤系统中反复低能量心内膜电除颤对左室舒缩功能无明显损伤作用;研究结果为经静脉多极导管自动心室除颤系统在临床的应用和电生理研究提供了可靠的实验数据。 相似文献