Ultraviolet Laser-Induced Fluorescence Spectroscopy Diagnosis of Human Stomach Malignant Tissues

To evaluate the potential of laser-induced autofluorescence spectroscopy for the detection of premalignant lesions of human stomach, fluorescence properties of stomach tissues have been investigated in vitro and in vivo. A specially made optical fibre probe and the multichannel fluorescence collection system have been used successfully in our research. Paper received 26 June 1997; accepted in revised form 31 October 1997.     

“C”型曲线的中耳声学特性改变与鼓室积液的关系

对40耳声导抗检查为“C”型曲线患耳进行中耳系统共振频率点测试并进行鼓室穿刺抽液,结果表明:共振频率点低于600Hz,患耳鼓室积液阳性率为82.1%,高于600Hz患者鼓室和液阳性率16.7%,提示依据鼓室导抗图和共振频率点测定可初步判断“C”型曲线患耳鼓室积液的可能.     

Qualitätssicherung in der Therapie chronischen Schmerzes

Ohne Zusammenfassung The online version of the original article can be found at     

肺肿瘤细胞雌激素、孕激素和凝集素受体的组化研究

用酶联亲合组化法对46例肺肿瘤细胞进行了雌激素受体(ER)和孕激素受体(PR)检测。同时测定14例肺瘤肿患者血清雌二醇(E_2)浓度。结果表明:肺肿瘤 ER 阳性率为36.9%,PR 阳性率为13.0%。阳性率与性别、病理类型及血浆 E_2浓度无关。认为肺癌的发生发展可能与雌激素有依赖关系,提示内分泌疗法可能会成为原发肺肿瘤的一种治疗手段。另用 ABC 法对上述46例肺肿瘤中的29例进行了花生凝集素受体(PNA-R)和麦胚凝集素受体(WGA-R)的检测,PNA-R 在腺癌大都为顶浆型分布,鳞癌和透明细胞癌则多为胞膜型分布。结果提示 PNA-R 是肺癌分化过程中的一种标志,对肺癌分型、预后判断有一定协助作用。同时将29例肺肿瘤 PNA-R 与 ER 状况作对比研究,探讨了二者之间的相关性及可能的发生机制。     

金属离子及氨基糖对大鼠肾脏N－乙酰氨基葡萄糖转移酶Ⅲ活性测定的影响

采用反相ＨＰＬＣ荧光测定法对大鼠肾脏Ｎ－乙酰氨基葡萄糖转移酶（ＧｎＴ）Ⅲ活性测定时二价金属离子及氨基糖的影响进行了研究。结果表明：ＧｎＴⅢ必须有二价金属离子激活，最佳激活离子为Ｍｎ￣（２＋）离子，其次为Ｍｇ￣（２＋）离子；二协金属离子对ＧｎＴⅢ的激活作用依次为Ｍｎ￣（２＋）＞Ｍｇ（２＋）＞Ｃｏ（２＋）＞Ｃａ￣（２＋）＞Ｎｉ￣（２＋）＞Ｂａ￣（２＋）＞Ｚｎ￣（２＋）。四种氨基糖（Ｎ－乙酰氨基葡萄糖ＧｌｃＮＡｃ，Ｎ－乙酰氨基半轧糖ＧａＩＮＡｃ，氨基葡萄糖ＧｌｃＮ，氨基半乳糖ＧａｌＮ），除ＧｌｃＮ使ＧｎＴ活性增加外，其余三种对ＧｎＴⅢ均有不同程度的抑制作用。     

Microsurgical restoration of distal digital function

Advances in microsurgery have produced multiple options for microsurgical restoration of distal digital function. However, because of the complexity of most mutilating hand injuries, these possibilities can be readily overlooked. A surgical awareness of these options for "like" tissue transplantation is now necessary for complete mutilating hand injury care. These "minor" aesthetic and functional microsurgical procedures can be major factors in overall patient acceptance and utilization of the reconstructed hands.     

Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices

While attempting to find a suitable crosslinking reagent for biopolymers, a naturally occurring proanthocyanidin (PA) obtained from grape seeds was selected to fix biological tissues. The cytotoxicity and crosslinking rate, reflected by the in vitro and in vivo degradation of fixed matrices has been studied. The shrinkage temperature of the fixed bovine pericardium increased from 66 to 86 degrees C. A cytotoxicity assay using fibroblast cultures revealed that PA is approximately 120 times less toxic than glutaraldehyde (GA), a currently used tissue stabilizer. In vitro degradation studies showed that fixed tissue was resistant to digestion by bacterial collagenase. Crosslinks between PA and tissues can be stabilized by decreasing the dielectric constant of the solution during storage. After subcutaneous implantation for periods ranging between 3 and 6 weeks, we found no apparent degradation of the GA- or PA-fixed tissues, whereas fresh tissue controls rapidly disintegrated. Beyond 6 weeks PA crosslinks began to degrade. More fibroblasts migrated and proliferated inside the PA-fixed implants compared with GA counterparts. Tissues crosslinked with PA manifested an enhanced collagen expression and deposition and did not calcify after implantation. GA, on the other hand, even after thorough rinsing continued to be cytotoxic, inhibited collagen synthesis and encouraged dystrophic calcification. Collagen matrices crosslinked with PA are expected to be of value in the design of matrices that will encourage cell ingrowth and proliferation, which are temporary in nature, and that are intended to regenerate or replace missing tissues, which can delay the biogradation of collagen. As such they should be of significant value in the emerging field of tissue engineering.     

Attenuated amplitude of circadian and sleep-dependent modulation of electroencephalographic sleep spindle characteristics in elderly human subjects

The low density lipoprotein receptor-related protein (LRP) gene is a candidate gene for Alzheimer's disease (AD) due to its role as a receptor for apolipoprotein E (apoE), a major genetic risk factor for late-onset familial and sporadic AD. Recently, several studies have reported a correlation between a polymorphism (C766T) in exon 3 of LRP and AD. We examined this polymorphism in a Caucasian population of 225 neuropathologically confirmed cases with AD and 187 elderly cases without any AD neuropathological changes. We found that the exon 3 LRP C/C genotype was slightly but not significantly higher in the AD group when compared to the control group. A meta-analysis of previous studies revealed only a weak correlation of this polymorphism with AD (odds ratio 1.34, [95% CI 1.16-1.54], P < 0.0001). These data indicate that the polymorphism in exon 3 of LRP is only a minor risk factor for AD and that another locus on chromosome 12 is likely responsible for the associations observed in other studies.     

运动应激对大鼠胃肠道5-HT免疫反应细胞的影响

目的　探讨力竭游泳对大鼠胃肠道 5 HT免疫反应细胞 (5 HTIR细胞 )的影响。方法　本研究以力竭游泳大鼠为运动模型 ,游泳后即刻取大鼠胃窦、十二指肠、空肠和回肠用免疫组织化学SP法检测 5 HT ,用图像分析系统测定胃肠 5 HTIR细胞数和平均灰度。结果　 (1)游泳后胃窦 5 HTIR细胞数虽与对照组无明显差异 ,但阳性细胞的平均灰度却明显下降 (P <0 .0 5)。 (2 )十二指肠、空肠、回肠 5 HTIR细胞数都明显下降 ,与对照组相比有显著性差异 (P <0 .0 5) ,但只有空肠 5 HTIR细胞平均灰度明显增加 ,与对照组间有明显差异 (P <0 .0 5)。结果　胃肠道不同区域 5 HTIR细胞以不同的反应方式应答运动应激。     

基于线性调频信号的高帧率超声成像系统

基于有限衍射波束的高帧率超声成像系统能实现快速成像，但由于仅通过一次发射事件成像，信噪比较低，本研究针对该问题提出一种改进方案。它采用合成孔径雷达中所使用的线性调频信号作为激励信号，在接收端则利用线性调频信号的脉冲压缩比等于信号的时间带宽积的特征，将接收信号通过匹配滤波器处理。结果表明该方案不仅能显著提高成像系统的信噪比，改善重构图像的质量，增加成像深度，而且不损失分辨率。