Persistent Dysregulation of IgA Production and IgA Nephropathy in the B6C3F1 Mouse Following Withdrawal of Dietary Vomitoxin (Deoxynivalenol)

To assess whether vomitoxin-induced dysregulation of IgA productionand IgA nephropathy are reversible, relevant immunologic parameterswere compared among experimental groups of B6C3F1 mice thatwere fed: (1) 25 ppm vomitoxin in AIN-76A semipurified dietfor 24 weeks (treatment group), (2) 25 ppm vomitoxin for 8 weeksand then control diet for 16 weeks (withdrawal group), and (3)control diet for 24 weeks (control group). Levels of serum IgAand microhematuria index in the treatment group were elevatedafter 4 to 8 weeks and continued to increase with further vomitoxinexposure. IgA immune complexes and mesangial IgA deposition,as quantitated by interactive laser cytometer image analysis,were also increased with toxin exposure at Weeks 8, 16, and24, whereas IgM, IgG, and complement component C3 depositionwere unaffected or depressed. Serum IgA, microhematuria index,and mesangial IgA deposition in withdrawal mice remained elevatedover those of the controls at Weeks 16 and 24 but were lessthan those of the treatment group. Cell recovery from Peyer'spatches (PP) as well as the percentages of IgA⁺ and CD4⁺ cellsin PP and spleen at Weeks 16 and 24 were greater in treatmentmice than in controls, but only the percentage of IgA⁺ cellsin PP was elevated in the withdrawal mice at these the sametime points. When IgA secretion by unstimulated and LPS-stimulatedsplenic lymphocytes was used as the measure of systemic production,it was elevated in both treatment and withdrawal mice at Weeks16 and 24. The results indicated that experimental dysregulationof IgA production and IgA nephropathy persisted up to 4 monthsafter a discrete period of dietary vomitoxin exposure, but thatthe severity of these effects did not increase in a progressivefashion.     

Influence of growth hormone-insulin-like growth factor Ⅰ axis on normal pregnancy

Objective To investigate the role of growth hormone-insulin-like growth factor Ⅰ (IGF-Ⅰ) axis in normal pregnancy. Methods Totally, 116 normal pregnant women were recruited from January 1997 to June 1998, with 20 normal nonpregnant women as controls. Maternal growth hormone (GH) and IGF-Ⅰ concentrations were assayed by RIA and enzyme-linked immunosorbent assay, respectively. Results Maternal serum levels of GH increased throughout gestation, reached a peak at 25 weeks of pregnancy and remained fairly high (χ[2]=40.458, P<0.0001).There was a significant difference between samples at 5-9 week gestational age and the controls (3.45 μg/L vs 1.61 μg/L, P<0.05). The maternal serum levels of IGF-Ⅰincreased rapidly throughout gestation from 29-week gestation and reached a peak of 188.86 μg/L at term delivery (χ[2]=50.224, P<0.0001). Conclusions Maternal GH levels increased progressively throughout gestation, which correlated with fetal growth. Maternal GH may regulate nutrition supply among mother, placenta and the fetus and play an important role in transporting nutritional substrates by the placenta. The maternal IGF-Ⅰin the third trimester may promote fetal growth and placental functions.     

应用经颅多普勒检测对椎-基底动脉供血不足原因的探讨

我们收集 1 990～ 1 999年共 5 0 0例临床确诊为椎 基底动脉供血不足 (Ｖｅｒｔｅｂｒｏｂａｓｉｌａｒｉｎｓｕｆｆｉｃｉｅｎｃｙ ,ＶＢＩ)的中老年病人 ,采用经颅多普勒超声 (ＴＣＤ)检测对该病病因进行探讨 ,结果报告如下。1　资料与方法　　 5 0 0例受检者中男性 2 85例 ,女性 2 1 5例 ,平均年龄 5 6 .4岁。病程 8ｄ至 2 0年 ,均以发作性眩晕起病。主要表现为反复发作性眩晕、头昏、呕吐、耳鸣、听力减退、视物模糊、四肢麻木、共济失调等症状。其它检查发现该组病例具有颈椎病、高血压、脑动脉粥样硬化症、高脂血症、高粘血症等。以…     

国产全热解碳双叶瓣的动物实验

目的 :探讨人造瓣膜动物实验的方法 ,并对国产瓣的性能作动物体内评价。方法 :体外循环下行羊的二尖瓣替换术 ,先作预实验以确定实验方法 ,后作正式实验。用心导管和超声 ,并用多巴酚丁胺负荷法模拟运动条件 ,分别测定人造瓣的血流动力学参数 ;根据动物存活情况及有无血栓形成和血栓栓塞等评价其生物相容性。结果 :预实验 10只羊均死亡 ,主要与瓣环撕裂有关 ;正式实验 5只羊均存活。术中和术后 2 .5年用导管法测得平均跨瓣压差分别为 0 .69± 0 .2 3k Pa、0 .81± 0 .0 4k Pa;动物尸检未见血栓形成和血栓栓塞。结论 :瓣环撕裂是本组手术主要死亡原因 ,选择适当体重的动物和保留瓣叶的折叠缝补 ,及术后合理的呼吸支持等是实验的关键 ;该国产瓣在动物体内血流动力学和抗血栓性能优良     

煤快速加氢热解焦油组成的分析

采用溶剂萃取－化学处理－柱层析相结合的预处理分离程序、色－质联用和色－红联用结合色谱保留值的定性方法,分析研究了内蒙东胜煤快速加氢热解焦油的化学成分和结构,推测鉴定出２００多种化合物,并对具有代表性的１９种多环芳烃进行了定量分析,讨论了快速加氢热解焦油化学成分的结构特征。     

CT scanning and arthroscopic evaluation of patellofemoral malalignment

Ｐａｔｉｅｎｔｓｗｉｔｈｐｅｒｉｐａｔｅｌｌａｒｐａｉｎｆｒｅｑｕｅｎｔｌｙｈａｖｅｕｎｄｅｒｌｙｉｎｇｐａｔｅｌｌｏｆｅｍｏｒａｌｍａｌａｌｉｇｎｍｅｎｔ Ｅａｒｌｙｄｉａｇｎｏｓｉｓ,ｈｏｗｅｖｅｒ,ｍａｙｂｅｄｉｆｆｉｃｕｌｔｂｅｃａｕｓｅｏｆａｐｏｏｒｃｏｒｒｅｌａｔｉｏｎｂｅｔｗｅｅｎｔｈｅｐａｔｈｏｌｏｇｉｃａｌｃｈａｎｇｅｓｏｆｔｒａｃｋｉｎｇａｂｎｏｒｍａｌｉｔｉｅｓａｎｄｔｒａｄｉｔｉｏｎａｌｒａｄｉｏｇｒａｐｈｉｃｖｉｅｗｓ Ｉｎａｄｄｉｔｉｏｎ ,ｒａｄｉｏｇｒａｐｈｉｃｅｘａｍ…     

血液血细胞比容血沉纤维蛋白原同血流变学相关指标检测的分析

本文通过临床常用的HCT、FIb、ESR与血流变中全血粘度、血浆粘度和红细胞聚集性之间相关性的实验对照观察、理论分析 ,认为血细胞比容 ,血沉和纤维蛋白原检测反映血液浓度、粘度、聚集、凝血状态是有一定相关价值。因此 ,再配合临床必要的生化实验 ,如血糖、血脂等检测采用微机对检测数据处理对心脑血管疾病的预测、疗效观察具有更实用的价值     

晶状体和玻璃体切除术后二期前房型人工晶状体植入

探讨晶状体,玻璃体切除术后二期前房型人工晶状植入的适应证,手术方法和疗效。方法,对我院行晶状体,玻璃体切除术后的２６例,分别在术３月－２年,行前房型人晶状体植入,术后随访６月以上。     

超声测量胎儿LL/FSTT预测胎儿体重

目的：探讨应用B型超声测量胎儿肝脏长度与胎儿股骨皮下组织厚度的比值（LL/FSTT）预测胎儿出生体重的临床价值。方法：用B型超声对400例胎儿双顶径、头围、腹围、股骨长度、肝脏长度与股骨皮下组织的厚度进行测量并分析与新生儿出生体重的关系。结果；肝脏长度与股骨皮下组织厚度的比值和胎儿体重密切相关（r=0.901）。对正常胎儿体重、巨大儿、胎儿宫内发育迟缓（IUGR）诊断的敏感性分别是88％、89％、86％，特异性分别是90％、92％、88％。结论：用胎儿肝脏长度与股骨皮下软组织厚度的比值预测胎儿体重的方法简单、准确，为临床掌握分娩时机和分娩方式提供可靠参考数据。     

SPECT脑血流显像在难治性癫癎灶定位诊断中的价值

目的 :探讨单光子发射计算机断层(SPECT)脑血流显像在癫灶定位诊断 ,指导癫外科治疗的价值。方法 :对88例难治性癫患者进行了脑电图(EEG) ,磁共振成像(MRI)和SPECT脑血流显像检查 ,其中42例患者进行了外科(开颅手术或γ刀放射)治疗。结果 :SPECT脑血流显像的阳性率最高 ,为90.9 % ,EEG和MRI的阳性率分别为76.1 %和40.9 %。外科治疗患者SPECT与EEG定位结果的一致及相对一致率为83.3 % ,SPECT与MRI定位结果的一致及相对一致率为81.3 %。3者定位结果的一致及相对一致率为75.0 %。外科治疗的有效率为83.3 %。结论 :SPECT脑血流显像是诊断癫灶灵敏而有效的方法 ,对正确选择癫手术或γ刀治疗方案并取得较好的治疗效果具有重要意义