电针对大鼠海马兴奋性突触后电位长时程增强的作用

目的 观察电针对麻醉状态下正常和东莨菪碱引起的学习记忆减退模型大鼠海马突触EPSP长时程增强(LTP)的作用。方法 引导大鼠海马齿状回颗粒细胞层突触后兴奋性电位群(EPSPs)，强直刺激(HFS)大脑皮层前穿质区引起海马突触LTP反应；用东莨菪碱制备学习记忆障碍模型；观察电针大椎和肾俞穴对正常和模型大鼠海马LTP的影响。结果 电针对HFS诱发的海马突触LTP效应，其作用强于未电针组，部分参数和时段有统计学意义(P＜0．05)，且维持时间长于后者；东莨菪碱i．p可显著抑制HFS诱发的海马突触LTP(P＜0．01)，电针能显著对抗这一抑制作用(P＜0．01；P＜0．05)。结论 电针对HFS引起的海马突触LTP有一定的易化作用，并对东莨菪碱引起的学习记忆障碍有显著的对抗作用。     

Stable Transfection of Nonosteogenic Cell Lines with Tissue Nonspecific Alkaline Phosphatase Enhances Mineral Deposition Both in the Presence and Absence of β-Glycerophosphate: Possible Role for Alkaline Phosphatase in Pathological Mineralization

It is documented that alkaline phosphatase (AP) plays an important role in bone mineralization. Considering that TN-AP is expressed in periodontal ligament fibroblasts, renal epithelial cells, and vascular endothelial cells, and that TN-AP is both a calcium-/phosphate-binding protein and a phosphohydrolytic enzyme, we hypothesize that membrane-bound AP also plays an important role in the initiation of physiological and pathological mineralizations in tissues other than bone and cartilage. To test this hypothesis, nonosteoblast cell lines, including a fibroblast line, a renal epithelial line, and a capillary endothelial line, were stably transfected to express high levels of rat bone AP on their cell surfaces. These rat bone AP-expressing cells were then cultured on filter membranes in the presence or absence of β-glycerol phosphate. von Kossa staining for calcium phosphate and transmission electron microscopy with electron diffraction analysis for minerals were employed to investigate the effect of membrane AP on extracellular calcium phosphate mineralization. Our results indicated that AP expression on these nonosteoblast-like cell surfaces have induced extracellular hydroxyapatite (HAP) mineralization. Our findings support the concept that membrane-bound AP contributes to extracellular apatitic mineralization by mechanisms that do not necessarily involve its hydrolase activity. They also suggest that AP might be important for the initiation of pathological mineralization in nonosteogenic tissues. Received: 11 January 1996 / Accepted: 31 October 1996     

心电图短P-R间期观测研究

目的：探讨和鉴别心电图短Ｐ－Ｒ间期的产生原因，为临床诊断、治疗提供依据。方法：检测１１５例受检者的常规导联心电图（ＲＬＥＣＧ）、头胸导联心电图（ＨＣＥＣＧ）、食管导联心电图（ＥＬＥＣＧ），对其中５５例临床可疑心律失常和ＲＬＥＣＧ示短Ｐ－Ｒ间期者行食管心房调搏电生理检查。结果：①Ｐ波出现率和振幅均值比较ＥＬＥＣＧ＞ＨＣＥＣＧ＞ＲＬＥＣＧ（Ｐ＜０．０１），出现率分别为１００％、９５％、９１％。②ＨＣＥＣＧＰ－Ｒ段均值（Ｐ＜０．０１）及变异系数＜ＲＬＥＣＧ。③ＲＬＥＣＧ波幅低、图形多变是产生心电图短Ｐ－Ｒ间期的主要原因。结论：作为无创伤性检查方法，ＨＣＥＣＧ和ＥＬＥＣＧ可以对短Ｐ－Ｒ间期者进行初步筛选，而经食管心房调搏能检测ＥＣＧ短Ｐ－Ｒ间期的电生理特性，对鉴别诊断有肯定价值。     

Identification of urinary metabolites of 8-methyl-8-azabicyclo- 3,2,1] octan-3-yl 3,5-dichlorobenzoate (MDL 72,222) in the dog and monkey.

The metabolism of 8-methyl-8-azabicyclo- 3,2,1]octan-3-yl 3,5-dichlorobenzoate (MDL 72,222) was studied in the dog and monkey. Four urinary metabolites were detected by HPLC, HPLC/MS, and GC/MS, and were identified by comparison to authentic standards. The major metabolite in the dog, approximately 41% of the administered dose excreted between 0 and 120 hr, was the MDL 72,222-N-oxide. On the other hand, the major metabolite in the monkey was the glycine conjugate of 3,5-dichlorobenzoic acid (greater than 56% of the dose). Seven percent of the dose in the monkey urine was free 3,5-dichlorobenzoic acid. N-Desmethyl MDL 72,222 was present at 2.5 and 1% in the dog and monkey, respectively. Very little (less than 1%) of the parent compound was found in urine. The major pathways of metabolism of MDL 72,222 are N-oxidation, N-demethylation, ester hydrolysis, and amino acid conjugation.     

蝮蛇抗栓酶对骨内高压降压作用实验研究

本实验随机将家兔一侧后肢于膝关节伸直位夹板外固定５周，制成固定侧胫骨上端骨内高压模型。对其全身和局部血液流变学指标进行测定，发现全血粘度，血浆粘度增大，血沉增快，血浆纤维蛋白原含量明显增高。对上述模型应用蝮蛇抗栓酶（ｓｖａｔｅ）连续治疗２１天，骨内压降低，血液流变学各项指标亦降低。本实验结果显示，蝮蛇抗栓酶通过改善骨内高压状态下的异常血液流变学状态可使骨内高压降低，从而认为血液流变学疗法可能成为临床治疗骨内高压症的一种新手段，而蝮蛇抗栓酶可能为其中高效药物之一。     

放射性碘在大鼠甲状腺内的滞留模式及剂量估算

为确定放射性碘在甲状腺内滞留模式，观察甲状腺吸收放射性碘后的生物效应，进而评价其辐射危害。方法利用活体测量方法，直接测量两个不同年龄组大鼠甲状腺内１３１Ⅰ的代谢参数。对测量数据进行处理后得到了大鼠甲状腺内１３１Ⅰ的滞留函数为三指数项函数。结果利用所得三指数项滞留函数的准确方法进行剂量估算，所得的两组动物的甲状腺平均累积吸收剂量分别为７．７Ｇｙ和１１．５Ｇｙ，而一般方法算得的大鼠甲状腺的平均累积吸收剂量比用准确方法高估了６６％～９１％。结论由于准确方法考虑到影响估算甲状腺所受剂量的一些因素，故可相对准确地反映了１３１Ⅰ所致不同年龄大鼠甲状腺的吸收剂量。     

Spinal cord injury: prognosis for ambulation based on quadriceps recovery.

The purpose of this study was to determine if early recovery of quadricep muscle strength post spinal cord injury (SCI) is a useful predictor of future ambulation. Seventeen C4-T10 motor incomplete (Frankel C) spinal cord injured patients admitted to our center between March 1988 and April 1990 were examined within 72 hours to one week post injury. All patients had initial quadricep strengths < or = 2/5 in both legs. Strength in the strongest quadricep was followed prospectively at intervals from admission to one year post injury. Recovery time to a > 3/5 quadricep was established for each patient. Patients were categorized into 2 groups: FA (n = 11) were those patients who achieved functional ambulation and NA (n = 6) were those subjects who were nonambulators. Functional ambulators were defined as those patients who were able to walk in the household and/or the community while non ambulators were those who either did not ambulate or did so only for exercise. All patients (n = 9) who achieved a > 3/5 quadricep by 2 months post SCI became functional ambulators whereas in the group of 8 patients who did not achieve a > 3/5 by 2 months, only 2 became functional ambulators. This result was found to be significant using a point-by-serial correlation with p < 0.05. In conclusion, motor incomplete spinal cord injured patients who recovered to a > 3/5 quadricep strength by 2 months post injury had an excellent prognosis for subsequent ambulation by 6 months post injury.     

颈阔肌的应用解剖

用手术显微镜对50例成人的颈阔肌作了观察和测量。将每侧颈部分为9个区,颈阔肌位于前6区。肌的内侧界约相当于颏舌骨连线中点稍下到锁骨胸骨端稍外侧处的连线,左右侧肌夹有40°角;外侧界为下颌角稍后到锁骨肩峰端内侧3cm处的连线;下界在第一肋间隙平面,有的肌下份出现一些较宽的裂隙;上界在下颌骨体,并与某些表情肌相连续。肌的面积约150cm~2,锁骨上部约占120cm~2。后份肌纤维略呈S形弯曲。肌与深层结构联系较松。本文还对面神经和颈丛与颈阔肌有关的分支,进行了观察。对和颈面部临床应用等有关的问题进行了讨论。     

看病难和看病贵问题:基于居民角度的实证分析

从居民角度对上海市看病难和看病贵问题进行了实证分析.研究共调查了上海市长宁区的3636位居民.居民调查结果进一步印证了整体研究中患者调查的部分结果,同时也发现了一些与患者调查不同的结果,提示患者调查存在的局限性.