全文获取类型
收费全文 | 46258篇 |
免费 | 2855篇 |
国内免费 | 195篇 |
专业分类
耳鼻咽喉 | 532篇 |
儿科学 | 1239篇 |
妇产科学 | 965篇 |
基础医学 | 6711篇 |
口腔科学 | 3580篇 |
临床医学 | 3828篇 |
内科学 | 9311篇 |
皮肤病学 | 962篇 |
神经病学 | 3861篇 |
特种医学 | 944篇 |
外国民族医学 | 2篇 |
外科学 | 4950篇 |
综合类 | 490篇 |
一般理论 | 13篇 |
预防医学 | 5501篇 |
眼科学 | 573篇 |
药学 | 3377篇 |
中国医学 | 402篇 |
肿瘤学 | 2067篇 |
出版年
2023年 | 406篇 |
2022年 | 1028篇 |
2021年 | 1711篇 |
2020年 | 1167篇 |
2019年 | 1464篇 |
2018年 | 1698篇 |
2017年 | 1229篇 |
2016年 | 1272篇 |
2015年 | 1499篇 |
2014年 | 2035篇 |
2013年 | 2424篇 |
2012年 | 3662篇 |
2011年 | 4009篇 |
2010年 | 2045篇 |
2009年 | 1604篇 |
2008年 | 2735篇 |
2007年 | 2748篇 |
2006年 | 2361篇 |
2005年 | 2094篇 |
2004年 | 1871篇 |
2003年 | 1686篇 |
2002年 | 1518篇 |
2001年 | 777篇 |
2000年 | 802篇 |
1999年 | 659篇 |
1998年 | 313篇 |
1997年 | 216篇 |
1996年 | 188篇 |
1995年 | 179篇 |
1994年 | 164篇 |
1993年 | 133篇 |
1992年 | 306篇 |
1991年 | 277篇 |
1990年 | 278篇 |
1989年 | 256篇 |
1988年 | 233篇 |
1987年 | 178篇 |
1986年 | 176篇 |
1985年 | 168篇 |
1984年 | 136篇 |
1983年 | 109篇 |
1982年 | 73篇 |
1979年 | 120篇 |
1978年 | 100篇 |
1974年 | 85篇 |
1973年 | 92篇 |
1970年 | 89篇 |
1969年 | 94篇 |
1968年 | 77篇 |
1967年 | 87篇 |
排序方式: 共有10000条查询结果,搜索用时 24 毫秒
71.
Razeq Shetab Stuart H. Cohen Thomas Prindiville Yajarayma J. Tang Mary Cantrell Darush Rahmani Joseph Silva Jr. 《Journal of clinical microbiology》1998,36(6):1729-1732
Bacteroides fragilis constitutes about 1% of the bacterial flora in intestines of normal humans. Enterotoxigenic strains of B. fragilis have been associated with diarrheal diseases in humans and animals. The enterotoxin produced by these isolates induces fluid changes in ligated intestinal loops and an in vitro cytotoxic response in HT-29 cells. We developed a nested PCR to detect the enterotoxin gene of B. fragilis in stool specimens. After DNA extraction, a 367-bp fragment was amplified with two outer primers. The amplicon from this reaction was subjected to a second round of amplification with a set of internal primers. With these inner primers, a 290-bp DNA fragment was obtained which was confirmed as part of the B. fragilis enterotoxin gene by Southern blotting with a nonradioactive internal probe and a chemiluminescence system. By this approach, B. fragilis enterotoxin gene sequences were detected in eight known enterotoxigenic human isolates and nine enterotoxigenic horse isolates. No amplification products were obtained from DNA extracted from 28 nonenterotoxigenic B. fragilis isolates or B. distasonis, B. thetaiotaomicron, B. uniformis, B. ovatus, Escherichia coli, or Clostridium difficile. The sensitivity of this assay allowed us to detect as little as 1 pg of enterotoxin DNA sequences or 100 to 1,000 cells of enterotoxigenic B. fragilis/g of stool. Enterotoxin production of all isolates was confirmed in vitro in HT-29 cells. A 100% correlation was obtained between enterotoxin detection by cytotoxin assay and the nested PCR assay. This rapid and sensitive assay can be used to identify enterotoxigenic B. fragilis and may be used clinically to determine the role of B. fragilis in diarrheal diseases. 相似文献
72.
73.
The impact of sexual harassment on girls' and women's developing concept of their body and self has rarely been examined with the goal of expanding our understanding of weight and shape preoccupation. In this paper we examine how this pervasive form of violence contributes to young women's uneasiness about their developing bodies and results in a disruption of healthy female development that often gets played out in disordered patterns of eating and intense body monitoring. 相似文献
74.
Paul R. Finley R. Jane Williams Carla Fletcher 《Journal of clinical laboratory analysis》1988,2(4):249-255
We have devised assays to detect both circulating alloantibodies to platelets (indirect assay) and platelet-association IgG and IgM (direct assay) using a flow cytometric technique. A variety of patients with immune thrombocytopenia were studied. Employment of a confocal lens in the flow cytometer increased the discrimination power of the instrument. Patients with autoimmune thrombocytopenia (idiopathic thrombocytic purpura [ITP], systemic lupus erythematosus (SLE), lymphoma, leukemia, and drug-induced thrombocytopenia showed a significant increase in platelet-associated antibody. Circulating antibodies to platelets (alloantibodies) were demonstrated in cases of platelet refractoriness and neonatal isoimmune purpura. Day-today precision of the assays ranged from 3% to 6% (coefficient of variation). No interference was shown in the presence of hemoglobin (5 g/L), triglycerides (10 g/L), or polyclonal and monoclonal immunoglobulinemia (50 g/L: IgG, IgA, IgM). The sensitivity of the direct assay was 500 attograms of IgG or IgM platelet. 相似文献
75.
C. M. A. Pennartz R. F. Ameerun H. J. Groenewegen F. H. Lopes da Silva 《The European journal of neuroscience》1993,5(2):107-117
Extra- and intracellular recordings in slices were used to examine what types of synaptic plasticity can be found in the core of the nucleus accumbens, and how these forms of plasticity may be modulated by dopamine. Stimulus electrodes were placed at the rostral border of the nucleus accumbens in order to excite primarily infralimbic and prelimbic afferents, as was confirmed by injections of the retrograde tracer fluoro-gold. In extracellular recordings, tetanization induced long-term potentiation (LTP) of the population spike in 20 out of 53 slices. The presynaptic compound action potential did not change following LTP induction. For the intracellularly recorded excitatory postsynaptic potential, three types of synaptic plasticity were noted: long-term potentiation (16 out of 54 cells), decremental potentiation (eight cells) and long-term depression (LTD; six cells). No correlation was found between the occurrence of potentiation or depression and various parameters of the tetanic depolarization (e.g. peak voltage, integral under the curve). The N -methyl- d -aspartate receptor antagonist d (–)-2-amino-5-phosphonopentanoic acid (50 μM; d -AP5) reduced, but did not completely prevent, the induction of LTP. The incidence of LTD was not markedly affected by d -AP5. No difference in LTP was found when comparing slices bathed in dopamine (10 μM) and controls. Likewise, slices treated with a mixture of the D1 receptor antagonist Sch 23390 (1 μM) and the D2 antagonist S (–)-sulpiride (1 μM) generated a similar amount of LTP as controls. In conclusion, both LTP and LTD can be induced in a key structure of the limbic-innervated basal ganglia. LTP in the nucleus accumbens strongly depends on N -methyl- d -aspartate receptor activity, but is not significantly affected by dopamine. 相似文献
76.
Mariana Diniz Bisi Santos Arthur Braga Pfeifer Marcos Rogério Pupo Silva Claudio Luiz Sendyk WIlson Roberto Sendyk 《Journal of applied oral science : revista FOB》2007,15(2):148-151
One of the causes of implant failures in cemented implant-retained prostheses is the fracture of abutment screw or UCLA abutment. This article reports a case of simultaneous fracture of two UCLA abutments screws occurring in an implant-supported prosthesis placed in the mandibular molar region. The fractured structures were examined under scanning electron microscopy to investigate the probable causes of the failure, which were not related to failures on materials or fabrication of the screws, but rather were due to shear forces. The misfit in cemented prostheses may be the most likely cause of shear force generation. 相似文献
77.
78.
M Salim-Hanna A M Edwards E Silva 《International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ern?hrungsforschung. Journal international de vitaminologie et de nutrition》1987,57(2):155-159
Studies in animals have suggested that the products of the irradiation of tryptophan in the presence of riboflavin may play a role in the development of hepatic dysfunction during parenteral nutrition. In this paper we describe the formation of an adduct between tryptophan and riboflavin obtained as a consequence of an anaerobic irradiation of these compounds. Through the use of molecular sieves and of an ion-exchange resin it was possible to separate the photo-adduct from the dimer riboflavin and other reaction products. The various fractions were characterized on the basis of their absorption and emission spectra. Also used were measures of anisotropy of fluorescence emission in order to characterize the derived adduct. 相似文献
79.
80.
Luiz H Castro Luiz K Ferreira Leandro R Teles Carmen L Jorge Paula R Arantes Carla R Ono Carla C Adda Rosa F Valerio 《Seizure》2007,16(1):50-58
PURPOSE: Hypothalamic hamartoma (HH) related epilepsy presents with gelastic seizures (GS), other seizure types and cognitive deterioration. Although seizure origin in GS has been well established, non-GS are poorly characterized. Their relationship with the HH and cognitive deterioration remains poorly understood. We analyzed seizure type, spread pattern in non-GS and their relationship with the epileptic syndrome in HH. METHODS: We documented all current seizure types in six adult patients with HH-epilepsy with video-EEG monitoring, characterized clinical-electrographic features of gelastic and non-gelastic seizures and correlated these findings with cognitive profile, as well as MRI and ictal SPECT data. RESULTS: Only four seizure types were seen: GS, complex partial (CPS), tonic seizures (TS) and secondarily generalized tonic-clonic seizures (sGTC). An individual patient presented either CPS or TS, but not both. GS progressed to CPS or TS, but not both. Ictal patterns in GS/TS and in GS/CPS overlapped, suggesting ictal spread from the HH to other cortical regions. Ictal SPECT patterns also showed GS/TS overlap. Patients with GS-CPS presented a more benign profile with preserved cognition and clinical-EEG features of temporal lobe epilepsy. Patients with GS-TS had clinical-EEG features of symptomatic generalized epilepsy, including mental deterioration. CONCLUSIONS: Video-EEG and ictal SPECT findings suggest that all seizures in HH-related epilepsy originate in the HH, with two clinical epilepsy syndromes: one resembling temporal lobe epilepsy and a more catastrophic syndrome, with features of a symptomatic generalized epilepsy. The epilepsy syndrome may be determined by HH size or by seizure spread pattern. 相似文献