1－取代－7－〔3－（乙胺基甲基）－1－吡咯烷基〕－6，8－二氟－1，4－二氢－4－氧喹啉－3－羧酸N_1位定量构效关系的量子化学研究

本文利用ＣＮＤＯ／２研究了１－取代－７－〔（３－（乙胺基甲基）－１－吡咯烷基〕－６，８－二氟－１，４－二氢－４－氧喹啉－３－羧酸Ｎ１位不同取代基的药效构象，首次报道了以第四最低空轨道能（ＦＵＭＯ），超离域度、前沿电子密度等指标与Ｎ１位取代基的立体摩尔长度、最小最大宽度等结合的Ｎ１位定量构效关系，稍优于文献用经验参数ＵＮＳＡＴ的结果，研究表明Ｎ１位的活性不仅与立体摩尔长度有关，还与电性效应有关，支持了Ｄｏｍａｇａｌａ以不饱和度参数（ＵＮＳＡＴ）所得的结论，但ＵＮＳＡＴ的意义不十分明确，本研究的量化指标意义很清楚。     

三种不同免疫抑制剂对胰岛细胞功能的影响

目的 观察免疫抑制剂对体外培养胰岛细胞活力的影响，探讨免疫抑制剂对胰岛移植物功能的影响。方法 应用体外纯化后培养的SD大鼠胰岛细胞，采用噻唑蓝法测定在不同浓度免疫抑制剂作用下胰岛细胞活力的变化。结果 低剂量雷帕霉素对胰岛细胞活力无明显影响，1ng/ml以上雷帕霉素能引起胰岛细胞活力降低；低剂量和高剂量daclizumab和FTY720对胰岛细胞的活力均无明显影响。结论 雷帕霉素对胰岛细胞有直接损伤作用，这种损伤程度与浓度呈正相关，daclizumab和FTY720对胰岛细胞无明显毒性。     

翼状胬肉术后的角膜地形图改变

目的 评价翼状胬肉术前术后的角膜屈光状态及其与术前胬肉大小的关系，探讨胬肉手术的时机与必要性。方法 对29例(32只眼)翼状胬肉患者进行胬肉侵入角膜的长度测量，并于术前术后行角膜地形图检测，以资对比。结果 32只眼均有程度不同的顺规散光，其大小与翼状胬肉侵入角膜的长度有关。手术显著降低了角膜散光，提高了角膜平均屈光力。手术导致的角膜散光变化、角膜平均屈光力变化和术前胬肉大小显著相关。结论 翼状胬肉的生长和手术切除对角膜的屈光状况有显著影响，胬肉越大，这种影响就越大。当胬肉较大时，角膜地形图的改变可能包括一部分假象。     

NOV蛋白免疫反应阳性神经元在鲢、鸡和几种哺乳动物脑的比较发育

目的：研究鲢、鸡、牛、犬和大鼠脑肾母细胞瘤过度表达基因（ｎｏｖ）蛋白免疫反应阳性神经元的比较发育。方法：免疫组织化学技术。结果：低等脊椎动物鲢脑仅有少量ＮＯＶ蛋白免疫反应阳性神经元，分布于延髓的一些核团、丘脑前核、丘脑后核、下丘脑背侧核、下丘脑外侧核。鸡脑ＮＯＶ蛋白免疫反应阳性神经元除广泛分布于脑干外，小脑、间脑和大脑纹状体的多数脑区阳性神经元亦较多。哺乳动物牛、犬和大鼠脑ＮＯＶ蛋白免疫反应阳性神经元广泛分布，脑干、小脑、间脑和大脑都检测到强烈阳性信号。结论：ｎｏｖ基因在从低等脊椎动物到高等脊椎动物的进化过程中非常保守，提示它在脑神经元生长、分化及正常生理功能中可能具有重要作用     

Molecular cytogenetic characterization of Thinopyrum and wheat--Thinopyrum translocated chromosomes in a wheat--Thinopyrum amphiploid

The wheat--Thinopyrum amphiploid 'Agrotriticum # 3425' (AT 3425), which is highly resistant to Cephalosporium stripe, was identified to carry seven pairs of Thinopyrum chromosomes, three pairs of wheat--Thinopyrum translocated chromosomes and 18 pairs of wheat chromosomes. Fluorescence genomic in situ hybridization (FGISH), C-banding, sequential C-banding and FGISH, and denaturing polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize and identify the chromosomes. The Thinopyrum chromosomes in AT 3425 were designated as T1 through T7 based on their C-banding patterns. The FGISH and C-banding patterns of mitotic chromosomes in AT 3425 and meiotic chromosomes in the hybrid between AT 3425 and wheat cultivar 'Chinese Spring' (CS) revealed that wheat chromosomes 1D, 2B and 3D were involved in the three wheat-Thinopyrum chromosome translocations designated as (W-T)1, (W-T)2, and (W-T)3 respectively. The analysis of high-molecular-weight glutenin subunits in single seeds of AT 3425 confirmed the involvement of wheat chromosome 1D in the translocation (W-T)1. The designations 1DSuu.1DL-1TL, 2BSuu.2BL-2TL and 3DSuu.3DL-3TL were suggested for the wheat--Thinopyrum translocated chromosomes (W-T)1, (W-T)2 and (W-T)3 in AT 3425 respectively.     

人工心脏起搏器的植换

探讨人工心脏起搏器植换方式选择。方法：起搏器植换２２例，其中，能源耗竭１４例，感知和起搏功能障碍４例，囊袋感染破溃致ＰＭ外露４例。非感染者起搏阈值，〈２．５Ｖ，行原位植换：ＰＴ值〉３．０Ｖ或有感染者，更换全套起搏系统。结果：非感染的１８例中，１０例ＰＴ〈２．５Ｖ，实行原位植换；８例ＰＴ值〉３．０Ｖ和其余４例有感染者，植入新的起搏系统。     

计算机在医学领域中的应用及展望

对计算机在医学文献检索、医学统计软件包的编制、疾病诊断、医院管理等医学领域中的应用作了简要的综述，并对未来的发展进行了预测。     

血管内皮细胞生长因子抑制剂对成骨肉瘤生长的影响

通过动物接种方法研究血管内皮细胞生长因子 (VEGF)抗体对骨肉瘤生长的抑制作用。将OS 732骨肉瘤细胞以 1 0 70 / (mL·只 )的剂量接种于BALB/C型裸鼠腋窝部皮下 ,并分成 4组 :A组于接种次日于瘤体局部注射VEGF抗体 ,B组于接种后 2周瘤体局部注射抗体 ,C组于接种次日于瘤体局部注射PBS ,D组不注射抗体。VEGF注射剂量为 2 0 0 μg/ (只·次 ) ,每周 3次 ,共 3周。 4周后处死A、C2组裸鼠 ,6周后处死B、D 2组裸鼠。每组均取出瘤组织作病理切片。计算肿瘤体积及肿瘤内微血管密度 ,并对结果进行分析。结果显示 ,A组的肿瘤体积小于B、C、D 3组 ,在接种后 2 5d与D组有统计学差异 (P <0 .0 5 ) ;B、C、D 3组间无统计学差异。 4组肿瘤内微血管密度的比较 ,无统计学差异。提示 ,VEGF抗体对骨肉瘤早期的生长有明显的抑制作用 ,可作为转移病灶的预防及治疗方法之一。     

Excretion of 1,2,4-benzenetriol in the urine of workers exposed to benzene

Urine samples were collected from 152 workers (64 men, 88 women) who had been exposed to benzene, 53 workers (men only) exposed to a mixture of benzene and toluene, and 213 non-exposed controls (113 men, 100 women). The samples were analysed for 1,2,4-benzentriol (a minor metabolite of benzene) by high performance liquid chromatography. The time weighted average solvent exposure of each worker was monitored by diffusive sampling technique. The urinary concentration of 1,2,4-benzentriol related linearly to the intensity of exposure to benzene both in men and women among workers exposed to benzene, and was suppressed by toluene co-exposure among male workers exposed to a mixture of benzene and toluene. A cross sectional balance study in men at the end of the shift of a workday showed that only 0.47% of benzene absorbed will be excreted into urine as 1,2,4-benzenetriol, in close agreement with previous results in rabbits fed benzene. The concentration of 1,2,4-benzenetriol in urine was more closely related to the concentration of quinol than that of catechol. The fact that phenol and quinol, but not catechol, are precursors of 1,2,4-benzentriol in urine was further confirmed by the intraperitoneal injection of the three phenolic compounds to rats followed by urine analysis for 1,2,4-benzenetriol.     

国产脑复康对家兔颅内压影响的研究

应用侧脑室插管方法,观察静脉注射脑复康对正常和已经增高的家兔颅内压的影响。实验结果提示,脑复康有降低家兔颅内压的作用,并且对硝酸甘油引起的颅内压升高有明显的防治作用。