中重度前臂缺血性肌肉挛缩晚期功能重建的手术治疗

目的 探讨中、重度前臂缺血性肌肉挛缩晚期功能重建的手术方法及疗效。方法 对42例中、重度前臂缺血性肌肉挛缩的晚期患者，进行旋前畸形矫正和旋后对掌功能重建手术。其中6例行指浅、深屈肌腱交叉延长术，17例行肌腱转位术＋骨间膜、旋前方肌松解术，19例行肌腱转位术＋骨间膜、旋前方肌松解术＋腕屈肌旋后功能重建术。结果 术后42例患者均获得6～34个月的随访。旋后功能恢复：优9例，良18例，可12例，差3例，优良率为64．3％。手功能恢复：优11例，良15例，可14例，差2例，优良率为61．9％。结论 对中、重度晚期缺血性肌肉挛缩的患者，应针对其不同的挛缩程度采用不同的手术方法进行治疗。     

PC12细胞基础状态氧化蛋白质表达谱

目的绘制基础状态下PC12细胞氧化蛋白质的表达谱,期望为以PC12细胞为模型的实验研究提供基本理论依据。方法采用2D凝胶电泳和westernblot技术相结合的方法获得氧化修饰的蛋白点,运用MALDI-TOF质谱鉴定出被氧化的蛋白质。结果PC12细胞总蛋白中共有91个蛋白点发生氧化修饰,运用MALDI-TOF质谱鉴定出19个氧化蛋白质。结论处于基础状态的PC12细胞存在蛋白质的氧化修饰,可以为病理条件下的氧化损伤研究提供可靠的参照系统。     

脑血管病诊治研究新策略探索及其评介

脑卒中作为发病率、致残率最高的疾病，一直备受广泛的瞩目，随着脑卒中诊治研究不断地深入发展进步，近年来的新认识与探索呈现出爆炸式增长。我们在此扼要评介脑血管病诊治研究中热点问题及其相关新策略，以期引起同道们更深入地关注。     

外科治疗高血压脑出血60例报告

我科自2002年6月至2005年6月共收治高血压脑出血60例,现将治疗结果总结报告如下。一、对象与方法1.一般资料:本组男37例,女23例;年龄42~70岁,平均55.5岁;患者均有高血压病史。2.血肿部位及出血量:血肿部位:基底节区出血36例,皮质下白质内出血20例,小脑出血4例,其中出血破入脑室     

高张盐水在局灶性脑缺血再灌注损伤中的作用及其机制

Objective To investigate the effect of hypertonic saline on cerebral water content, tumor necrosis factor-α (TNF-α) level and neuronal apoptosis following focal cerebral ischemia-reperfusion (IR) injury in rats and explore the mechanisms involved. Methods Ninety-six rats were randomized equally into 4 groups, namely the shame-operated group, untreated IR injury group, and 4.2% and 7.5% hypertonic saline groups (HS-A and HS-B groups, respectively). In the latter 3 groups, cerebral ischcmia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments. Serum sodium concentration was measured at 5 min before and at 30, 60 and 90 min after the reperfilsion. At 22 h of rcperfusion, the rats were sacrificed after neurological deficit evaluation, and brain edema was assessed by measuring the wet-to-dry weight ratio of the brain tissue. TNF-α expression in the ischemic brain tissue was measured by enzyme-linked immunosorbent assay (ELISA), and the neuronal apoptosis was analyzed using TUNEL assay. Results In the saline-treated rats, serum sodium level increased significantly after saline administration, lasting for 60 min before recovering the normal levels in HS-A group and for over 90 min in HS-B group. Compared with that in the sham-operated group, the brain water content in rats of the IR group increased in both of the hemispheres, but more obviously in the ischemic hemisphere. In the two saline-treated groups, the water content decreased significantly in the bilateral hemispheres, which was especially obvious in the ischemic hemisphere;administration of 7.5% saline resulted in greater water content reduction in the ischemic hemisphere than 4.2% saline. Compared with the IR group, the two saline-treated groups showed significant reduction in TNF-α levels and apoptotic cells in the brain along with decreased neurological deficits. Conclusion Hypertonic saline can ameliorate cerebral focal IR injury by decreasing the cerebral water content, TNF-α level and neuronal apoptosis following the injury.     

外伤性脱位前牙再植的临床美学观察及探讨

目的：观察外伤性脱位牙再植后的临床治疗效果,探讨提高再植成功率的临床经验。方法：53例患者65颗外伤性脱位牙患者行牙再植术,其中25例采用GBR植骨技术,临床随访观察1～5年。结果：65颗外伤性脱位牙再植成功53颗,成功率为81.5%,经植骨后美学效果满意度显著提高。结论：再植成功与就诊时间、保存方式关系密切,对于牙槽窝唇侧骨板有缺损的患者通过局部GBR植骨手术处理可以改善美学效果。     

复发性静脉性溃疡的原因分析及再处理方法

目的 分析复发性静脉溃疡的原因及再次手术策略.方法 回顾性分析2000年1月至2010年6月收治的复发性静脉性溃疡患者的临床资料.共73例患者81条肢体入组,男性55例(60条肢体),女性18例(21条肢体),平均年龄52.6岁(31～73岁).所有患者入院前均因下肢静脉溃疡接受过一次或数次手术.溃疡复发距上次手术8～37个月,平均10.6个月;溃疡直径1.3～6.5 cm,平均3.7 cm.再次手术前多普勒超声发现小腿交通静脉反流57条肢体(70.4%),原发性深静脉瓣膜功能不全38条肢体(46.9%),DVT后遗症16条肢体(19.8%),大隐静脉属支残留11条肢体(13.6%),大隐静脉主干再通或残留8例(9.9%).针对存在的病因采取大隐静脉高位结扎抽剥及属支结扎、内镜深筋膜下交通静脉结扎、溃疡周边环形缝扎、曲张静脉环形缝扎加切除、深静脉瓣膜修复术以及穿着弹力袜等治疗措施.结果 随访3～60个月,平均37.3个月.3个月时溃疡平均直径明显缩小,6个月时溃疡完全愈合,血流动力学指标明显改善.1年后3条肢体(3.7%)溃疡再次复发.结论 小腿交通静脉功能不全、深静脉瓣膜功能不全以及大隐静脉再通或属支残留是静脉性溃疡术后复发的主要原因,针对性的冉次手术仍然可以达到满意疗效.     

病灶清除加髓腔持续负压封闭引流治疗胫骨慢性骨髓炎

目的：探讨病灶清除加髓腔持续负压封闭引流对胫骨慢性骨髓炎的治疗效果。方法：2006年3月至2009年5月,采用彻底病灶清除,骨皮质开槽并髓腔内持续负压封闭引流治疗胫骨慢性骨髓炎19例,二次手术闭合创面,其中男12例,女7例;年龄25-68岁,平均39岁,均为创伤后导致的胫骨慢性骨髓炎,迁延不愈。病程10个月～5年。临床症状：局部软组织红肿、压痛、流脓,有经久不愈窦道及恶臭脓液流出。X线片示：骨质硬化,密度增高,有死骨、死腔或形成包壳。细菌培养：绿脓杆菌3例,金黄色葡萄球菌13例,产气杆菌1例,溶血性链球茵2例。其中3例为耐甲氧西林的金黄色葡萄球菌。结果：经清创、负压封闭引流18～22d,创面肉芽新鲜,直接缝合创面17例,行皮瓣移位修复创面2例,创面愈合标准是创面干燥、清洁,无渗液。所有创面I期愈合,观察6—12个月窦道无复发,X线片示骨质生长良好,没有发生骨折。结论：病灶清除加髓腔持续负压封闭引流是治疗胫骨慢性骨髓炎的一种安全、有效的方法．     

磷酸肌酸对糖尿病大鼠心肌缺血再灌注时细胞凋亡的影响

目的 探讨磷酸肌酸对糖尿病大鼠心肌缺血再灌注时细胞凋亡的影响.方法 雄性SD大鼠,体重150～170 g,采用高脂饲养联合腹腔注射链脲佐菌素的方法制备糖尿病模型,造模成功的27只大鼠饲养2周后,采用随机数字表法,将其随机分为3组(n=9):假手术组(S组)、缺血再灌注组(I/R组)和磷酸肌酸组(PP组).I/R组和PP组采用结扎左冠状动脉前降支30 min再灌注2 h的方法制备心肌缺血再灌注模型,PP组于缺血前30 min腹腔注射磷酸肌酸1g/kg,I/R组给予等容量生理盐水.再灌注2 h时采集静脉血样,测定血浆肌钙蛋白T(cTnT)的浓度,然后处死大鼠,取心肌组织,采用免疫组化法测定Bcl-2、Bax和Caspase-3表达的水平,并计算Bcl-2与Bax表达的比值(Bcl-2/Bax 比),采用TUNEL染色法检测细胞凋亡情况,计算凋亡指数(AI);电镜下观察心肌细胞超微结构.结果 与S组比较,I/R组血浆cTnT浓度升高,心肌组织Bcl-2、Bax和Caspase-3表达上调,Bcl-2/Bax比降低,AI升高(P＜0.05或0.01);与I/R组比较,PP组血浆cTnT浓度降低,心肌组织Bcl-2表达上调,Bax和Caspase-3表达下调,Bcl-2/Bax比升高,AI降低(P＜0.01).PP组心肌病理学损伤程度轻于I/R组.结论 磷酸肌酸可抑制细胞凋亡,从而减轻糖尿病大鼠心肌缺血再灌注损伤,其机制与上调Bcl-2表达、下调Bax和Caspase-3的表达有关.

Abstract：

Objective To investigate the effects of phosphocreatine on apoptosis following myocardial ischemia-reperfusion (I/R) in diabetic rats. Methods Male SD rats weighing 150-170 g were used in this study.Diabetes mellitus was induced by high fat diet and intraperitoneal streptozotocin. Twenty-seven rats in which diabetes mellitus was successfully induced were randomly divided into 3 groups ( n = 9 each): sham operation group (group S);myocardial I/R group(group I/R )and phosphocreatine group (group PP). Myocardial I/R was induced by 30 min occlusion of left anterior descending branch of coronary artery followed by 2 h reperfusion in I/R and PP groups. In group PP phosphocreatine 1 g/kg was given intraperitoneally 30 min before myocardial I/R. Blood samples were collected at the end of 2 h reperfusion for determination of plasma concentration of calcium troponin T (cTnT). The animals were then sacrificed and iscbemic myocardial specimens were isolated. The expression of Bcl-2, Bax and Caspase-3 in isehemic myocarcdium was determined and Bcl-2/Bax ratio was calculated. Myocardial apoptosis was detected by TUNEL and apoptotic index was calculated. The ultrastructure of cardiomyocytes was examined with electron microscope. Results Myocardial I/R significantly increased plasma cTnT concentration and Bcl-2, Bax and Caspase-3 expression in myocardium and apoptosis index and decreased Bcl-2/Bax ratio. Phosphocreatine significantly attenuated I/R-induced above-mentioned changes and myocardial damage. Conclusion Phosphocreatine can reduce myocardial I/R injury in diabetic mellitus rats by reducing myocardial apoptosis through up-regulation of Bcl-2 expression and down-regulation of Bax and Caspase-3 expression.     

三种不同方法治疗瘢痕疙瘩的疗效比较

目的：比较单纯放射治疗,曲安奈德注射＋放射治疗与手术＋放射治疗瘢痕疙瘩的疗效。方法：回顾分析91例瘢痕疙瘩患者采用三种治疗方法的疗效,其中单纯放射治疗28例,曲安奈德注射＋放射治疗31例,手术＋放射治疗32例。结果：单纯放射治疗组治愈率为35.8%,有效率为67.9%;曲安奈德注射＋放射治疗组治愈率为64.5%,有效率为90.3%;手术＋放射治疗组治愈率为71.9%,有效率为93.8%。单纯放射治疗组的治愈率和有效率与曲安奈德注射＋放射治疗组以及手术＋放射治疗组比较差异均有统计学意义（P〈0.05）,曲安奈德注射＋放射治疗组与手术＋放射治疗组的治愈率和有效率比较差异均无统计学意义（P〉0.05）。结论：曲安奈德注射＋放射治疗与手术＋放射治疗对瘢痕疙瘩的疗效均优于单纯放射治疗,前两者疗效相当。