Effect of heat treatment on the antigenicity of bovine α-lactalbumin and β-lactoglobulin in whey protein isolate

The effect of heat treatment on the antigenicity of α-lactalbumin (α-LA) and β-lactoglobulin (β-LG) in whey protein isolate (WPI) was investigated at temperatures in the range of 50–120°C for times from 0.5 to 30 min. The antigenicity was determined by an indirect competitive enzyme-linked immunosorbent assay. The heat denaturation of α-LA and β-LG was revealed by SDS-PAGE electrophoresis. The antigenicity of α-LA and β-LG increased with increasing temperature from 50 to 90°C and the highest antigenicity of α-LA and β-LG was detected at 90°C. However, above 90°C the antigenicity of both proteins showed a remarkable decrease. When treated at 120°C for 20 min, the antigenicity of α-LA decreased below the initial value of the untreated sample. The antigenicity of α-LA and β-LG in WPI can be decreased at certain heat temperature and time.     

The Clinical Study and HLA Genotyping of 112 Familial Myasthenia Gravis Patients

Ｉｔｈａｓｂｅｅｎｗｅｌｋｎｏｗｎｔｈａｔｍｙａｓｔｈｅｎｉａｇｒａｖｉｓ（ＭＧ）ｉｓａＴｃｅｌ－ｍｅｄｉａｔｅｄｓｐｏｒａｄｉｃａｕｔｏｉｍｍｕｎｅｄｉｓｅａｓｅｄｉｒｅｃｔｅｄａｇａｉｎｓｔｎｉｃｏ－ｔｉｎｉｃａｃｅｔｙｌｃｈｏｌｉｎｅｒｅｃｅｐ...     

白藜芦醇对成纤维细胞分泌心钠素及脑钠素的影响

背景：白藜芦醇是从传统中药中提取的多酚类化合物，具有抑制成纤维细胞增殖作用。 目的：观察白藜芦醇对成纤维细胞分泌心钠素和脑钠素的影响，验证白藜芦醇对肌腱瘢痕成纤维细胞增殖的抑制作用。 设计、时间及地点：分组对照观察，与2007-03/12在广东医学院实验动物中心完成。 材料：雌性健康成年日本大耳白兔6只，体质量2.0~2.5 kg。白藜芦醇，中国药品生物制品鉴定所提供，纯度99%，用二甲基亚砜溶解成50 mmol/L储存液，置-20 ℃保存。 方法：采用差速贴壁法体外分离培养兔肌腱瘢痕成纤维细胞。将培养的细胞分为2组，对照组不加药物；白藜芦醇组又分为4个亚组，分别加入25，50，75，100 μmol/L白藜芦醇作用72 h。 主要观察指标：采用放免法及ELISA法检测细胞培养液中心钠素及脑钠素水平；反转录-聚合酶链反应方法检测脑钠素、心钠素mRNA表达；硝酸还原酶法测细胞培养液中一氧化氮水平；化学比色法测细胞上清液中一氧化氮合酶水平；放免法测定细胞内环磷酸鸟苷水平。 结果：白藜芦醇25~100 μmol/L作用细胞后一氧化氮、一氧化氮合酶及环磷酸鸟苷水平升高，心钠素、脑钠素水平降低，心钠素、脑钠素mRNA的表达下降，在一定范围内有明显的剂量及时间依赖性。 结论：白藜芦醇在一定浓度范围能降低培养液中心钠素及脑钠素水平，并抑制细胞中心钠素及脑钠素mRNA的表达。     

Myoepithelial carcinoma of the parotid gland with bilateral thyroid involvement: A case report and review of the literature

A patient was admitted to hospital with enlarged lobes of the thyroid gland with bilateral cervical lymph node involvement, and surgical excision followed. Histological examination of this specimen revealed a lesion that showed myoepithelial cell differentiation. Primary thyroid and skin appendage tumors were excluded based on clinical examination, conventional histology and immunohistochemistry. A tumor of the right parotid surgically treated in June 2008, approximately 2 years previously, was originally classified as a basal cell adenocarcinoma with focal invasion, but was re-examined. Using immunohistochemistry, the parotid tumor was re-classified as a myoepithelial carcinoma. The thyroid tumor proved to be metastasis from a primary parotid tumor, which was not found in the updated review of the literature. The literature is reviewed, including current knowledge on the histological and immunohistochemical features of myoepithelial carcinoma, with limited data on treatment suggestions.     

某医院应用项目管理的启示

介绍医院应用项目管理的3个案例,总结项目管理运行经验,提出要处理好项目管理运行机制与现有运行机制的若干冲突,把握3个类型项目的组织运行要点,为医院开展项目管理应用提供参考。     

纯音声场测听可行性研究报告

本文的目的是探讨纯音用于声场测听的可行性。普遍认为声场测听适宜刺激为啭音和窄带噪声，而纯音仅作为耳机测听和消声室中测试信号。本文通过对纯音声压分布、声场变异以及频率漂移对参考点声压级影响的研究发现，扩散场中纯音声压分布的均匀性虽不如啭音，但在某些范围其能量仍可充分扩散，这些区域声场测试结果受受试者头位改变和／或频率漂移影响轻微。对２０名重度感音神经性耳聋患者的测试结果证实了上述结论。由此认为，如在声场校正基础上合理选择参考点位置，用纯音进行声场测听是可行的。     

HPLC法测定金银花及利咽止咳冲剂中绿原酸的含量

采用高效液相色谱法测定金银花及其制剂“利咽止咳冲剂”中绿原酸的含量。以ＫＹＷＧ－Ｃ＿（１８）为固定相，水－乙腈－冰醋酸（１１０：３：３）为流动相，检测波长为３２３ｎｍ，绿原酸在０．０１～０．１０ｍｇ／ｍｌ浓度范围与峰面积呈直线关系，回归方程为Ｙ＝一２．４６０５ｘ１０３＋５．６１３８×１０￣３Ｘ，ｒ＝０．９９９８。平均回收率为１００．８７％，ＲＳＤ为１．５５％（ｎ＝６）。本法简便，快速，结果可靠。