全文获取类型
收费全文 | 3187篇 |
免费 | 275篇 |
国内免费 | 303篇 |
专业分类
耳鼻咽喉 | 51篇 |
儿科学 | 43篇 |
妇产科学 | 25篇 |
基础医学 | 415篇 |
口腔科学 | 54篇 |
临床医学 | 455篇 |
内科学 | 500篇 |
皮肤病学 | 59篇 |
神经病学 | 253篇 |
特种医学 | 87篇 |
外国民族医学 | 1篇 |
外科学 | 318篇 |
综合类 | 442篇 |
一般理论 | 2篇 |
预防医学 | 201篇 |
眼科学 | 70篇 |
药学 | 366篇 |
5篇 | |
中国医学 | 105篇 |
肿瘤学 | 313篇 |
出版年
2024年 | 8篇 |
2023年 | 40篇 |
2022年 | 100篇 |
2021年 | 118篇 |
2020年 | 90篇 |
2019年 | 96篇 |
2018年 | 120篇 |
2017年 | 76篇 |
2016年 | 85篇 |
2015年 | 146篇 |
2014年 | 143篇 |
2013年 | 143篇 |
2012年 | 179篇 |
2011年 | 245篇 |
2010年 | 151篇 |
2009年 | 116篇 |
2008年 | 140篇 |
2007年 | 239篇 |
2006年 | 178篇 |
2005年 | 179篇 |
2004年 | 133篇 |
2003年 | 124篇 |
2002年 | 108篇 |
2001年 | 94篇 |
2000年 | 81篇 |
1999年 | 108篇 |
1998年 | 53篇 |
1997年 | 48篇 |
1996年 | 45篇 |
1995年 | 31篇 |
1994年 | 24篇 |
1993年 | 23篇 |
1992年 | 45篇 |
1991年 | 40篇 |
1990年 | 38篇 |
1989年 | 22篇 |
1988年 | 26篇 |
1987年 | 17篇 |
1986年 | 11篇 |
1985年 | 6篇 |
1984年 | 11篇 |
1983年 | 11篇 |
1982年 | 6篇 |
1981年 | 5篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1975年 | 5篇 |
1973年 | 9篇 |
1968年 | 4篇 |
1966年 | 4篇 |
排序方式: 共有3765条查询结果,搜索用时 31 毫秒
101.
Serrano D Carrión R Balsalobre P Miralles P Berenguer J Buño I Gómez-Pineda A Ribera JM Conde E Díez-Martín JL;Spanish Cooperative Groups GELTAMO GESIDA 《Experimental hematology》2005,33(4):487-494
OBJECTIVE: To evaluate feasibility, safety, and efficacy of peripheral blood stem cell collection (PBSCC) and autologous stem cell transplantation (ASCT), to treat patients diagnosed of high-risk or relapsed HIV-associated lymphoma (HIV+ Ly), responding to highly active antiretroviral therapy (HAART). METHODS: Prospective and multicentric study in patients with high-risk or relapsed chemosensitive HIV+ Ly, candidate for consolidation with ASCT. Eligibility criteria were similar to those of HIV- lymphoma. HAART was aimed to be maintained during the procedure. RESULTS: Fourteen patients were admitted. Adequate PBSCC was obtained from all patients (median CD34+ cells was 4.7 x 10(6)/kg). Three patients died before ASCT; two had disease progression and one died from VHC-liver failure. Eleven transplanted patients showed neutrophil engraftment after a median time of 16 days (range, 9-33 days), and nine patients showed platelet engraftment after a median time of 20 days (range, 11-36 days). CD4+ cell counts and HIV viral load (VL) were appropriately preserved along the procedure. No patients died from treatment-related complications. One patient died from lymphoma progression (day +19), and another died in complete remission (CR) with undetectable VL, 15 months after transplant, due to infection. One patient relapsed at 32 months after ASCT. The remaining eight patients are alive in CR with an event-free survival of 65% and a median follow-up of 30 months after ASCT (range, 7-36 months). CONCLUSIONS: These results show that feasibility, safety, and efficacy of PBSCC and ASCT in HIV+ Ly patients responding to HAART are similar to those observed in the HIV- lymphoma setting. 相似文献
102.
103.
Qiaojun Zeng Tiantian Tang Biru Huang Shiyi Bu Yingqi Xiao Yumeng Dai Zixin Wei Linjie Huang Shanping Jiang 《The Journal of international medical research》2021,49(4)
ObjectiveSingle nucleotide polymorphisms (SNPs) of pentraxin 3 (PTX3) are associated with various outcomes of lung infections. This study aimed to analyze the relationship between PTX3 polymorphisms and the severity of community-acquired pneumonia (CAP).MethodsThis is a retrospective case-control study comprising 43 patients with severe CAP (SCAP) and 97 patients with non-severe CAP. Three SNPs in the PTX3 gene (rs2305619, rs3816527, and rs1840680) from peripheral blood samples were genotyped by real-time polymerase chain reaction. The association between each SNP and the CAP severity was analyzed by logistic regression analysis.ResultsWe found that the rs1840680 polymorphism was significantly associated with CAP clinical severity. However, no such association was observed for the genotypes and allele frequencies of rs2305619 or rs3816527. The PTX3 rs1840680 AG genotype was an independent factor for a lower risk of SCAP after multivariate logistic regression analysis. Male sex and coronary heart disease were associated with an increased risk of SCAP.ConclusionsThe PTX3 rs1840680 AG genotype was found to be associated with a lower risk of SCAP, and may serve as a potential protective biomarker to help clinical judgment and management. 相似文献
104.
NA Hanchard DR Murdock PL Magoulas M Bainbridge D Muzny YQ Wu M Wang AL McGuire JR Lupski RA Gibbs CW Brown 《Clinical genetics》2013,83(5):457-461
The advent of whole‐exome next‐generation sequencing (WES) has been pivotal for the molecular characterization of Mendelian disease; however, the clinical applicability of WES has remained relatively unexplored. We describe our exploration of WES as a diagnostic tool in a 3½‐year old female patient with a 2‐year history of episodic muscle weakness and paroxysmal dystonia who presented following a previous extensive but unrevealing diagnostic work‐up. WES was performed on the proband and her two parents. Parental exome data was used to filter potential de novo genomic events in the proband and suspected variants were confirmed using di‐deoxy sequencing. WES revealed a de novo non‐synonymous mutation in exon 21 of the calcium channel gene CACNA1S that has been previously reported in a single patient as a rare cause of atypical hypokalemic periodic paralysis. This was unexpected, as the proband's original differential diagnosis had included hypokalemic periodic paralysis, but clinical and laboratory features were equivocal, and standard clinical molecular testing for hypokalemic periodic paralysis and related disorders was negative. This report highlights the potential diagnostic utility of WES in clinical practice, with implications for the approach to similar diagnostic dilemmas in the future. 相似文献
105.
Fangkun Wang Zujian Qiao Sen Hu Wenxing Liu Huajun Zheng Sidang Liu Xiaomin Zhao Zhigao Bu 《Infection and immunity》2013,81(8):2812-2818
Brucella melitensis causes brucellosis, a disease affecting sheep, cattle, and sometimes humans. Attenuated B. melitensis strain M5-90, derived from virulent strain M28, is widely used as a live vaccine in ruminants in China. Genetic differences between the strains may cast light on the mechanism of attenuation. We recently reported the complete genomic sequences of M28 and M5-90. Genome organization is highly conserved between these isolates, and also with virulent strains 16 M and ATCC 23457. Analysis revealed 23 open reading frames (ORFs) with consistent differences between M5-90 and the virulent strains. Notably, the tuf2 gene encoding translation elongation factor EF-Tu from M5-90 contained 50 single nucleotide polymorphisms (SNPs) and 9 gaps (indels) compared to tuf2 of M28 or of the other virulent strains. There were no changes in tuf1. To evaluate the potential role of EF-Tu in pathogenesis, tuf1 and tuf2 mutants of M28 and an M5-90 strain harboring wild-type tuf2 were constructed, and their virulence/attenuation was evaluated in vivo. We report that the tuf2 gene plays an important role in the attenuation of M5-90 virulence. 相似文献
106.
Stela ?iv?i?-?osi? Mirela Bu?i? ?eljko ?upan Gordana Pel?i? Martina Anu?i? Juri?i? ?eljka Jur?i? Mladen Ivanovski Sanjin Ra?ki 《Croatian medical journal》2013,54(1):65-70
During the past ten years, the efforts to improve and organize the national transplantation system in Croatia have resulted in a steadily growing donor rate, which reached its highest level in 2011, with 33.6 utilized donors per million population (p.m.p.). Nowadays, Croatia is one of the leading countries in the world according to deceased donation and transplantation rates. Between 2008 and 2011, the waiting list for kidney transplantation decreased by 37.2% (from 430 to 270 persons waiting for a transplant) and the median waiting time decreased from 46 to 24 months. The Croatian model has been internationally recognized as successful and there are plans for its implementation in other countries. We analyzed the key factors that contributed to the development of this successful model for organ donation and transplantation. These are primarily the appointment of hospital and national transplant coordinators, implementation of a new financial model with donor hospital reimbursement, public awareness campaign, international cooperation, adoption of new legislation, and implementation of a donor quality assurance program. The selection of key factors is based on the authors'' opinions; we are open for further discussion and propose systematic research into the issue.Transplantation is a widely accepted and successful life-saving treatment providing the best therapeutic benefit for hundreds of thousands of patients (1). Unfortunately, many people die while awaiting an organ transplant. A global shortage of organs available for transplantation raises many bioethical concerns, including the dilemma how to allocate limited resources to an unlimited number of needs and thus offer a fair and equal access to organ transplantation to all patients. Great efforts have been made to increase organ donation worldwide, but with only a moderate success in most of the countries. In contrast with this general trend, Croatia has recently experienced a boom in organ donation and transplantation. In 2011, Croatia had the highest rates of utilized cadaveric donors, kidney transplantations, and liver transplantations in the world (2-5) (Figure 1).Open in a separate windowFigure 1The number of kidney transplantations and the number of patients on the waiting list in Croatia between 2008 and 2011.Remarkably, only one decade ago, Croatia was lagging far behind other European countries with a low donation rate (2.7 donors per million population [p.m.p.] in 2000). The continuous improvement of the organization of the Croatian transplant program resulted in a steadily growing donor rate, which reached the highest level in 2011, with 33.6 utilized donors p.m.p (2). We analyzed the factors that might have contributed mostly to this great success. 相似文献
107.
Elif Doğan Baki Mustafa Aldemir Serdar Kokulu Halit Buğra Koca Yüksel Ela Remziye Gül Sıvacı Nilgün Kavrut Öztürk Mustafa Emmiler Fahri Adalı Hanife Uzel 《Inflammation》2013,36(6):1327-1333
Cardiopulmonary bypass (CPB) contributes to the secretion of anti-inflammatory cytokines that mediate the inflammatory response observed during open heart surgery. In addition to many factors, type of anesthesia management affects immune response and central nervous system in cardiac surgery. The aim of this study was to assess the effect of propofol versus desflurane anesthesia on systemic immune modulation and central nervous system on patients undergoing coronary artery bypass grafting. Forty patients undergoing elective coronary artery bypass graft surgery with CPB were included in this prospective randomized study. Patients were allocated to receive propofol (n?=?20) or desflurane (n?=?20) for maintenance of anesthesia. The blood samples for IL-6, IL-8, TNF-α, and S100β were drawn just prior to the operation before the induction of anesthesia, second before cardiopulmonary bypass, third after CPB, fourth 4 h postoperatively at the ICU. Major finding in our study is that S100β levels were lower in propofol group when compared to desflurane anesthesia. And also immune reaction was less in patients exposed to desflurane anesthesia when compared to propofol anesthesia as indicated by lower plasma concentrations of IL-8 and IL-6. Propofol is more preferable in terms of S100β for anesthetic management for CABG. 相似文献
108.
109.
110.