P2X receptors in trigeminal subnucleus caudalis modulate central sensitization in trigeminal subnucleus oralis

This study investigated the role of trigeminal subnucleus caudalis (Vc) P2X receptors in the mediation of central sensitization induced in nociceptive neurons in subnucleus oralis (Vo) by mustard oil (MO) application to the tooth pulp in anesthetized rats. MO application produced a long-lasting central sensitization reflected in neuroplastic changes (i.e., increases in neuronal mechanoreceptive field size and responses to innocuous and noxious mechanical stimuli) in Vo nociceptive neurons. Twenty minutes after MO application, the intrathecal (i.t.) administration to the rostral Vc of the selective P2X(1), P2X(3), and P2X(2/3) receptor antagonist, 2'-(or 3'-)O-trinitrophenyl-ATP (TNP-ATP), significantly and reversibly attenuated the MO-induced central sensitization for more than 15 min; saline administration had no effect. Administration to the rostral Vc of the selective P2X(1), P2X(3), and P2X(2/3) receptor agonist, alpha,beta-methylene ATP (alpha,beta-meATP, i.t.) produced abrupt and significant neuroplastic changes in Vo nociceptive neurons, followed by neuronal desensitization as evidenced by the ineffectiveness of a second i.t. application of alpha,beta-meATP and subsequent MO application to the pulp. Administration to the rostral Vc of the selective P2X(1) receptor agonist beta,gamma-methylene ATP (beta,gamma-meATP, i.t.) produced no significant neuroplastic changes per se and did not affect the subsequent MO-induced neuroplastic changes in Vo nociceptive neurons. These results suggest that P2X(3) and possibly also the P2X(2/3) receptor subtypes in Vc may play a role in the initiation and maintenance of central sensitization in Vo nociceptive neurons induced by MO application to the pulp.     

渗透压、细胞容积与鼻咽癌细胞增殖

目的:研究渗透压、细胞容积与鼻咽癌细胞增殖的关系。方法:用MTT法检测在不同渗透压培养条件下低分化鼻咽癌细胞(CNE－2Z)的增殖能力,流式细胞仪测定细胞周期分布,活细胞图像分析测量细胞容积,台盼蓝拒染法检测细胞存活率。结果:高渗(370、440mOsmol/L)培养增大细胞容积和促进细胞增殖,细胞容积分别增大8.7%、27.8%,增殖率分别提高22.2%和33.9%;而低渗(160、230mOsmol/L)培养减小细胞容积和抑制增殖,细胞容积分别减小12.8%和4.1%,增殖率分别降低34.0%和15.6%;细胞容积与细胞增殖率呈正相关。非等渗长期培养条件下,细胞周期各时相分布没有显著差异。低渗培养降低细胞生存率。结论:胞外渗透压、细胞容积与鼻咽癌细胞增殖密切相关,低渗培养可能通过减小细胞容积、促进细胞死亡而抑制细胞增殖。     

Mucosal bromodomain-containing protein 4 mediates aeroallergen-induced inflammation and remodeling

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国内110株新生隐球菌临床株变种、基因型和交配型分析

目的 对国内部分地区的新生隐球菌(Cryptococcus neoformans)临床株进行分子流行病学调查,分析其变种、基因型和交配型的构成和分布.方法 (1)PCR指纹分型法:以野生型噬菌体M13中针对小卫星DNA的核心序列为单引物对模板进行PCR扩增,将所有受试菌株鉴定到8种主要基因型水平.(2)利用变种和交配型特异性引物扩增分型法,区分格鲁比变种(C.neoformans var.grubii)、新生变种(C.neoformans var.neoformans)和格特变种(C.neoformans var.gattii),同时鉴定α和a交配型.结果 110株临床株中,98株(89.1%)为格鲁比变种,均为VNI基因型和α交配型;9株(8.2%)格特变种,包括VGⅠ基因型、α交配型8株(7.3%)和VGⅡ基因型、α交配型1株(0.9%);2株(1.8%)为AD杂合体,VNⅢ基因型,-/α和α/-交配型各1株;1株(0.9%)为新生变种,VNⅣ基因型和a交配型.结论 我国新生隐球菌临床株包含3个变种和AD杂合体.与国外情况比较,相似的是国内临床株中绝大部分为α交配型菌株,且格鲁比变种中的VNⅠ基因型占了其中的大部分;但未发现VNⅡ、VGⅢ和VGⅣ基因型菌株.     

中药芦笋促T细胞有丝分裂活性的观察

低浓度芦笋原汁(0.1～1.0％)可明显促进正常小鼠胸腺细胞的增殖,也可刺激正常小鼠脾脏细胞的增殖,但对裸鼠脾脏细胞没有任何作用。由此提示,芦笋具有促T淋巴细胞有丝分裂的活性,而对B淋巴细胞没有作用。与常用的T细胞促有丝分裂素植物血凝素(PHA)及刀豆蛋白A(ConA)不同,芦笋原汁对人、绵羊、豚鼠和鸡的红细胞均无凝集作用。     

Prognostic value of the combined assessment of proliferating cell nuclear antigen immunostaining and nuclear DNA content in invasive human mammary carcinomas

The expression of theS-phase associated, nuclear protein proliferating cell nuclear antigen (PCNA) was investigated in routinely paraffin-embedded surgical specimens from 209 breast cancer patients. Cytometric DNA assessments were performed on fine-needle aspirates, upon which the primary diagnosis of breast cancer had been based. The mean clinical follow-up was 16 years (range 13–20 years). The percentage of PCNA immunoreactive tumour cells ranged between less than 5 to 60% (mean value 13.34%). There was a direct association between PCNA expression, high histological tumour grade (p<0.01), and DNA aneuploidy (p=0.009). In a subgroup of 22 patients with near-diploid DNA distribution patterns the PCNA expression yielded additional prognostic information. Patients with tumours of near-diploid DNA histograms and more than 20% of PCNA immunoreactive neoplastic cells had a significantly worse clinical course, than patients with neardiploid tumours containing less than 20% PCNA immunoreactive cells (p=0.0001). In contrast, the PCNA immunoreactivity did not yield additional prognostic information for patients with distinctly diploid or highly aneuploid tumour variants. In a multivariate analysis comprising all 209 patients, nodal status (p<0.01), tumour size (p<0.01), and DNA ploidy (p<0.01) were found to have significant prognostic effect. The findings indicate that carcinomas characterised by high proliferative activity and near-diploid DNA distribution patterns can show rapid tumour progression. The combined assessment of the PCNA immunoreactivity and of the nuclear DNA content in routinely processed surgical specimens of breast cancer patients appears to be of prognostic value.     

血管内皮生长因子反义基因转染对高转移人巨细胞肺癌细胞系生 …

目的 探讨反义血管内皮生长因子（ＶＥＧＦ）基因转染在抑制恶性肿瘤生长和转移的抗肿瘤血管基因治疗中的意义。方法 利用基因重组技术构建正义和反义ＶＥＧＦ１２１ ｃＤＮＡ真核表达载体,用脂质体法转染高转移性人巨细胞肺癌细胞（ＰＧ）,经Ｎｏｒｔｈｅｍ杂交和Ｗｅｓｔｅｒｎ印迹免疫化学检测ＶＥＧＦ ｍＲＮＡ和蛋白质的表达水平,并对转染前后细胞进行体外生长和裸鼠体内生长转移等多项生物学行为实验,结果 转染反义转     

耳穴静态电测量中的瞬变响应

耳穴电参数时变关系实验表明，在测量起始ｔ＜２τ时，因瞬变作用，电位Ｅ（ｔ）和压降Ｕ（ｔ）为瞬态响应，响应函数呈指数关系，特征参数为弛豫时间τ，τ≈ＲＣ；ｔ＞２τ时，为时变间期。电路分析给出数学描述，并与耳穴和模拟实验结果较相符。提示，时变特征应以ｔ＞２τ后提取，静态电测量时，采样应避开瞬变期，可提高准确性。该工作对正确鉴别时变性和特征提取，全面认识耳穴电特性具有重要意义。     

检测Epstein—Barr病毒特异性细胞毒性T淋巴细胞方？…

目的 建立一种非放射性、简便易行的可检测特异性细胞毒性Ｔ淋巴细胞的方法，并且初步应用于Ｅｐｓｔｅｉｎ－Ｂａｒｒ病毒的细胞免疫应答。方法 用重组的ＥＢＶ－ＬＭＰ１痘苗病毒、ＴＫ＾＋痘苗病毒和杆状病毒系统表达的ＥＢＶ－ＬＭＰ！蛋白分别免疫Ｂａｌｂ／Ｃ小鼠，用Ｐ８１５细胞和乳酸脱氢酶法检测ＥＢ病毒特异性细胞毒性Ｔ细胞的杀伤效应。结果 重组ＥＢＶ－ＬＭＰ１痘苗病毒免疫组原发ＣＴＬ水平和体外诱生的二次ＣＴＬ