髓过氧化物酶和过氧化氢酶基因多态性及其酶活力与燃煤污染型地方性砷中毒关系的探讨

Objective To detect genetic polymorphism of myeloperoxidase (MPO) gene and catalase (CAT) gene and their activities, and to analyze their relationship with arsenic poisoning caused by coal-burning. Methods One hundred and thirty arsenic poisoning patients were chosen as case group in Jiaole Village, Xingren County, Guizhou Province(an endemic area). One hundred and forty healthy residents living in 13 km away were chosen as control group. Their blood was collected. Polymerase chain reaction-restriction fragment length polymorphism technique(PCR-RFLP) was used to detect polymorphism of MPO-463G/A and CAT-262C/T. Ultraviolet spectmphotometer method was used to detect myeloperoxidase activity. Chromatometry method was used to detect catalase activity. Results The genotype frequency of MPO-463G/A at GG, GA, AA site was 47.24%(60/127), 44.09%(56/127),8.67% (11/127) in case group and 42.34% (58/137),48.17% (66/137)1,9.49% (13/137) in control group, respectively. The difference between the two groups was not significant(χ2 = 0.642, P > 0.05). The genotype frequency of CAT-262C/T, at CC, CT, TT site was 65.60%(82/125),28.80%(36/125),5.60%(7/125) in case group and 76.51%(101/132), 18.94% (25/132) ,4.55% (6/132) in control group, respectively, without significant difference (χ2 =3.845, P>0.05). The relationship between polymorphism of MPO-463G/A and CAT-262C/T and the risk of arsenic poisoning was not found in this study(ORadj= 1.36, 95%CI: 0.74-2.50 for MPO; ORadj=1.35, 95%CI: 0.69-2.63 for CAT). The activities of MPO and CAT were (25.30±8.70)U/L and (2.80± 1.09)×103 U/L in case group, while (22.76±7.59)U/L and (3.90±1.01)×103U/L in control group with a significant difference(F=0.760 for MPO, F=0.855 for CAT, all P < 0.05). The genotype of MPO-463G/A and CAT-262C/T was not found to have relationship with the activities of MPO, CAT(F=1.312,2.822 for MPO; F= 0.151,0.036 for CAT, P>0.05). Conclusions Genetic polymorphism of MPO-463G/A and CAT-262C/T is not found to have relationship with arsenic poisoning. Arsenic can lead to the change of MPO and CAT activity, which, however, may not be affected by MPO-463G/A and CAT-262C/T polymorphism.     

复方维甲酸软膏封包治疗砷剂角化病近期疗效观察

为了探讨燃煤型砷中毒患者砷剂角化病的外用药物疗法,我们配制了以维甲酸、水杨酸及二巯基丙醇为主的复方维甲酸软膏,与水杨酸、二巯基丙醇软膏进行临床对照研究。治疗组总有效率为 75.6％,对照组为 60.0％。两者比较,χ 2=4.097, P< 0.05,具有显著性意义。说明复方维甲酸软膏对治疗燃煤型砷中毒患者砷剂角化病具有较好的疗效。     

强化科技兴院意识 拓展医院发展空间

卫生工作的发展必须以科学技术作为依托。部队医院作为医疗卫生工作的重要组成部分,要发展也必须依靠科学技术,必须强化科技兴院意识,拓展医院发展空间。我院的做法是:注重“四求”。1 在思想上求高认识 医院要落实科技兴院,加快全面建设的步伐,首要问题是“讲政治”,把思想政治工作摆在首位,巩固“三讲”教育取得的成果,以邓小平同志“科学技术是第一生产力”的思想为指导,以军委新时期军事战略方针为依据,以提高部队战斗力为标准,贯彻落实     

碳酸锂对抗化学诱导大鼠肝癌前病变过程中GST-P的表达及意义

目的观察碳酸锂 (Li2 CO3)对抗黄曲霉毒素B1(AFB1)诱发的大鼠肝癌前病变的组织形态学变化及胎盘型谷胱甘肽硫转移酶 (GST P)的表达。方法采用肝癌发生的动物模型。 144只Wistar大鼠 ,随机分为A、B、C、D四组 (A组为阴性对照组 ;B组为阳性对照组 ;C组为Li2 CO3 同时给药组 ;D组为Li2 CO3 先期给药组 ) ,于实验第 6、9、10周分批断头处死动物 ,取动物肝脏进行肝组织学检查和GST P的免疫组化染色。结果C、D两组动物健康状况明显改善 ,肝癌前期病变程度明显减轻 ;肝癌标志酶GST P表达减弱 (P <0 0 1) ;D组动物一般状况明显好于C组 ,GST P阳性灶的下降趋势优于C组。结论Li2 CO3 对化学诱导肝癌有明显对抗作用 ,并有一定的预防作用。GST P的动态检测有助于肝癌的早期发现及追踪观察     

BCL-2及P53mt蛋白筛检砷中毒致皮肤癌的价值

目的：探讨BCL－2及P53mt蛋白筛检砷中毒致皮肤癌的价值。方法：BCL－2及P53mt蛋白折检测用免疫组化染色法；两指标阳性率的分析用X^2检验；分析评价筛价值的有关指标。结果：无论是P53mt还是BCL－2，癌变组的阳性率均高于非癌变组，差异均有统计学意义（P53mt，P＜0．01）；BCL－2，P＜0．05），其中OR值分别为14．22（2．93－68．97）和8．76（1．07－71．51）。P53mt的约登指数为0．529，明显高于BCL－2的0．284。串联试验可提高筛检价值。并联试验则降低了筛检价值。结论：P53mt与BCL－2均可用于筛检砷中毒所致皮肤癌，将两指标串联可提高筛检价值。     

民间验方"肿痒─抹灵"的药效学研究

肿痒一抹灵来源于民间古方,原名太白仙水、神风油。由天花粉、一枝蒿、白芷、白藓皮、祖师麻、冰片、樟脑等九味中药组成,经醇提取而成的外用酊剂。     

磺胺与抗组织胺药物在临床上的合用

一、用药对象:凡不能排除应用磺胺类药物后发生过敏反应者均可采用此法。二、用药方法:一般在使用磺胺前服抗组织胺药物,具体时间可根据药物的吸收和反应期而定。用药量均为临床常规剂量。如用长效磺胺0.5克,     

燃煤污染型砷中毒患者头发中微量元素与砷中毒关系探讨

目的:检测燃煤污染型砷中毒患者头发中微量元素含量,探讨其与砷中毒的关系,为砷的毒作用机制分析及辅助性诊断和干预提供参考依据.方法:采用原子吸收光谱法测定107例砷中毒患者和30例对照人群头发中的铜(Cu)、锰(Mn)、锌(Zn)、铁(Fe)、钙(Ca)、镁(Mg);原子荧光光谱法测定砷(As)、硒(Se)含量.结果:砷中毒患者发中的Se、Zn、Ca、Mg明显低于对照组;Cu、Fe、Mn、As明显高于对照组,差异均有统计学意义;砷中毒不同病情组Se、Zn和Cu、Fe、Mn、As等含量有随病情加重而降低或升高的趋势.结论:砷可影响人体内微量元素的平衡,微量元素的失衡可能是砷毒作用的重要机制之一.