Synergistic Anti-inflammatory Effect of Radix Platycodon in Combination with Herbs for Cleaning-heat and Detoxification and Its Mechanism

Objective:To investigate the synergistic anti-inflammatory effect of Radix Platycodon in combination with herbs for cleaning-heat and detoxification and its mechanism for Fei(肺)-targeting.Methods: Forty Wistar rats were randomly divided into five groups(8 per group):the sham-operated group,model group, Radix Platycodon group,Flos Lonicera and Fructus Forsythia(LF) group,and Radix Platycodon,Flos Lonicera and Fructus Forsythia combination(PLF) group,using a random number table.A rat chronic obstructive pulmonary disease(COPD) model was established by passive smoking and intratracheal instillation of lipopolysaccharide(LPS).The treatments started from the 15th day of passive smoking for a total duration of 14 days.At the end of the treatment,changes in the following measurements were determined:lung histopathology, inflammatory cytokines including tumor necrosis factorα(TNF-α),transforming growth factorβ(TGF-β) and interleukin IL-1β(IL-1β) in bronchoalveolar lavage fluid(BALF),and mRNA expression of endogenous active substance intestinal trefoil factor 3(TFF3) in the lung tissue.Results:Light microscopy showed that compared with the sham-operated group,rats in the COPD model group had disrupted alveolar structure,collapsed local alveoli,significantly widened or even fused alveolar septa,and massive infiltration of inflammatory cells in the alveolar wall and interstitium.In addition,significant bronchial epithelium hyperplasia,partially shed epithelia, and marked inflammatory cell infiltration in the bronchial wall and its surrounding tissues were noticed.Electron microscopy showed that rats in the model group had degeneration of alveolar type II epithelial cell;reduction, breakage or even loss of cell surface microvilli;swollen mitochondria with disappearing cristae and vacuole-like structure;and,increased secondary lysosomes in alveolar macrophages.The TNF-α,TGF-βand IL-1βlevels and white blood cell(WBC) count in BALF were significantly increased(P<0.01 or P<0.05) and TFF3 mRNA expression in the lung tissue was significantly reduced(P<0.01).After treatment,the pathological morphology of lung injury was less severe in all three treatment groups.In addition,TGF-βand IL-1βand WBC count in BALF were decreased(P<0.01 or P<0.05),and TFF3 mRNA expression in the lung tissue was significantly increased in the PLF group(P<0.01).Compared with the LF group,the IL-1 p in BALF was significantly decreased (P<0.05),and TFF3 mRNA expression was significantly increased(P<0.05) in the PLF group.Conclusions:Radix Platycodon synergizes with herbs for cleaning-heat and detoxification in reducing inflammatory injury in a rat model of COPD.The synergistic anti-inflammatory effect is reflected in the improvement in pathological changes and in the reduction of IL-1 p levels in BALF.The mechanism of such synergistic action may be related to its effect on maintaining the TFF3 mRNA expression and Fei-targeting function.     

前生长抑素原mRNA神经元在大鼠大脑皮质的生后早期发育──地高辛标记cRNA探针原位杂交的研究

前生长抑素原ｍＲＮＡ神经元在大鼠大脑皮质的生后早期发育──地高辛标记ｃＲＮＡ探针原位杂交的研究（湖北医科大学解剖学教研室，武汉４３００７１周兰仙，韩爱民，袁华，胡家荦，王丹，王晓云，章祖陈，王亚威）已有资料表明，生长抑素作为一种神经递质或调质，在脑的...     

刘景源教授辨治尿路结石经验述要

尿路结石一病为泌尿外科常见病与多发病。刘景源教授辨治尿路结石多以下焦湿热立论,循"化""导""推""排"四法,重调畅气血,制四金排石汤随症加减,药专效捷,颇具特色。文章从以上几个方面对刘景源教授临证经验做详细介绍,并附医案举隅。     

呼吸机相关性肺炎新生儿气管导管末端病原学分析

目的 分析新生儿呼吸机相关性肺炎(VAP)的病原学特点和药物敏感性,为新生儿VAP的临床治疗提供依据.方法 选择2009年12月-2012年6月在我院新生儿病房行呼吸机机械通气治疗并确诊为VAP的59例新生儿为研究对象,对气管导管末端的细菌培养结果和药敏试验结果进行回顾性分析.结果 59例新生儿共送检标本62份,55份导管末端细菌培养阳性,阳性率为88.7%.其中革兰阴性杆菌48份(87.3%),革兰阳性菌5份(9.1%),白色念珠菌2份(3.2%),培养阴性7份(11.3%).55种菌株均有不同程度的药物敏感率及耐药性,革兰阴性杆菌对药物有较好的敏感性,革兰阳性球菌对药物有较大的耐药性.结论 新生儿VAP的主要致病菌是以革兰阴性杆菌为主,革兰阳性菌较少见.     

旋动式人工流产器的研制

旋动式人工流产器是一种用于人工流产的新型手术医疗器械，它适用于早孕人工流产，实施流产术时，利用旋转运动将孕囊打破，缠绕，剥离并将其牵取出宫腔，400例手术均能顺利取出孕囊而终止妊娠，孕妇的疼痛感明显减轻，提高了人工流产的安全性和有效性，减少了副反应和并发症。     

Y染色体长臂缺失与不育及自然流产的遗传学分析

目的通过研究Y染色体进一步了解Y染色体长臂缺失与不育及自然流产的关系.方法通过我院门诊收集病例,采用外周血淋巴细胞培养技术对患者进行Y染色体核型分析.结果 89例无精子症Yq- 5例占5.62%.160例少精子症Yq- 14例占8.75%.460例自然流产Yq- 15例占3.26%.结论 Y染色体长臂缺失可导致Y染色体上睾丸决定基因和与精子生成发育的基因发生缺失易位突变等改变从而造成了男性性腺发育不全,不同程度上损害精子的发育和分化并可能导致精子异常,流产.     

基于慢性阻塞性肺疾病“从肠论治”的临床疗效评价设计方案要点探析

根据目前中医药治疗慢性阻塞性肺疾病临床研究的Meta分析结果,结合课题实践,提出中医理论指导下的因果关联科学假说的建立是临床研究的前提;为验证假说、客观评价"中医药治疗COPD有效性"这一命题,应选择符合现代临床科研方法的随机对照试验作为"金指标"设计方案,并对研究方案设计要点、临床疗效评价体系的构建、数据管理与分析等进行探讨。     

从“卫出下焦”探讨《伤寒论》对附子的运用

卫气根于下焦,卫气的生成、循行、发挥功能皆不离肾,卫气功能的强弱取决于肾气的盛衰.张仲景据“卫出下焦”理论创立温肾助卫、扶阳固表之治法,治疗卫气虚弱证或阳虚外感证,以(炮)附子温肾阳、充卫阳而固肌表.太阳底面,即是少阴,因此,在治疗太阳病的同时,须处处顾及少阴真阳.     

立足《伤寒论》探析扶阳派医案中姜附剂之服药反应

《伤寒论》对服药反应的记载,如服用辛温方剂后出现烦躁、衄血、口渴、下利等,看似症状加重,病情加剧,实则为服药后的正常反应。扶阳派医家深得张仲景辨治之妙,对服用姜、桂、附等温热药物的药后反应体会深刻,积累了丰富经验,临证之际应对自如,胆识过人。笔者立足《伤寒论》,就扶阳派医案中姜附剂之服药反应探析如下。     

李可古中医流派用药研究

目的 研究李可古中医流派的用药特点。方法 借助古今医案云平台等统计工具,运用数理统计方法,整理分析李可古中医流派代表性协定处方。结果 李可古中医流派临床处方,高频用药有炙甘草、人参、附子、茯苓、乌梅等48味,以甘味、平性药为主、整方药味多<13味,剂量集中在100～300 g之间。结论李可古中医流派参悟经典理论及李可思想,结合临床实际,具备药少力专、药量范围宽、重用甘味药等特点,其用药经验对现代临床具有重要的启发性。