吉兰-巴雷综合征患者的心理指导

目的了解吉兰—巴雷综综合症患者各个时期的心理状态,进行不同的心理指导,使其能够配合治疗与护理。方法不同的时期采取不同的心理护理。结果患者配合治疗与护理,康复出院。     

酸性鞘磷脂酶在类风湿关节炎中的研究进展

RA是一种慢性自身免疫病,发病机制尚未明确, 参与RA病理的关键分子可能成为治疗RA的靶点。酸性鞘磷脂酶(ASMase)是鞘脂类物质代谢中的一种关键酶, 参与多种疾病病理而成为治疗这些疾病的可能靶点。新近研究发现ASMase在RA的病理中起着关键作用, 可能作为治疗RA的新靶点。本论文将综述ASMase的结构和生理功能、活性或表达异常与相关疾病, 以及在RA中的研究进展, 为RA的治疗提供新靶点。     

无对照二分类数据的Meta分析在RevMan软件中的实现

本文介绍无对照二分类数据资料的2种效应指标及其标准误的计算方法,然后通过1个实例分别用上述2种方法计算效应指标及其标准误,将计算所得的效应指标及其标准误在RevMan软件中进行Meta分析,最后将计算结果与用实例的原始数据在Stata软件中的Meta分析结果进行比较。结果显示RevMan和Stata软件进行无对照二分类数据资料Meta分析的结果一致。     

TRAIL对人喉癌Hep-2细胞生长及凋亡的影响

目的:研究TRAIL对人喉癌Hep-2细胞生长及凋亡的影响,并探索其相关机制.方法:用MTT法和流式细胞仪检测不同浓度TRAIL对喉癌细胞Hep-2,成纤维细胞MRC-5生长及凋亡率的影响.用western blotting对比两种细胞DR-4,DR-5的表达情况.检测Hep-2细胞TRAIL干预前后caspase-8,caspase-3,caspase-9表达的变化.结果:随着TRAIL浓度的升高,Hep-2细胞存活率逐渐降低,而凋亡率逐渐提高.MRC-5细胞对TRAIL不敏感.Western blotting检测发现Hep-2细胞DR-4、DR-5表达明显高于MRC-5.TRAIL干预Hep-2细胞后caspase-8、caspase-3、caspase-9活化分解明显增多.结论:喉癌细胞Hep-2由于DR-4、DR-5的高表达,对TRAIL促凋亡作用具有特异敏感性,其促凋亡作用是通过激活线粒体依赖性通路实现的.     

坤泰胶囊用于治疗绝经综合征的临床体会

目的：探讨坤泰胶囊治疗绝经综合征的临床效果及临床体会。方法：将129例患有绝经综合征的妇女,随机分成两组,服用复方戊酸雌二醇（戊酸雌二醇1mg,炔诺酮0.35mg）为对照组,服用坤泰胶囊为治疗组。观察两组治疗期间Kupperman评分改善情况以及阴道流血、乳房胀痛的不良反应。结果：两组经药物治疗后,Kupperman评分改善显著。治疗组疗效显著（P〈0.05）。而阴道流血、乳房胀痛的不良反应治疗组较对照组为轻。结论：坤泰胶囊改善绝经期症状,疗效显著,并且乳房胀痛的发生率、胀痛程度、阴道出血率等不良反应显著低于雌激素,安全性好。     

CD44调控鼻咽癌上皮-间质转化及转移的实验研究

目的:研究鼻咽癌细胞中CD44与上皮-间质转化(EMT)及转移的相关性,并初步探讨CD44调控鼻咽癌细胞EMT及转移的机制。方法:Western blotting检测鼻咽癌细胞株5-8F和6-10B中CD44与EMT相关因子的表达。用带有CD44基因的质粒以脂质体瞬时转染鼻咽癌细胞株6-10B获得CD44高表达的鼻咽癌细胞。Western blotting检测转染前后细胞中CD44与EMT相关因子的表达。细胞划痕实验检测6-10B转染前后细胞迁移能力的改变。结果:鼻咽癌细胞株5-8F中CD44和EMT相关因子基质金属蛋白酶-9(MMP-9)的表达量高于6-10B,而EMT相关因子E-钙黏蛋白的表达量低于6-10B,5-8F的迁移能力强于6-10B。CD44基因转染后的6-10B中CD44及MMP-9的表达均升高。CD44基因转染后的6-10B迁移能力明显增强。结论:在鼻咽癌细胞中,CD44通过调控EMT进程影响鼻咽癌细胞的转移能力。     

逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

TrKB与VEGF在鼻咽癌组织中的表达及临床意义

目的探讨血管内皮生长因子(VEGF)、酪氨酸激酶B(TrKB)在鼻咽癌(NPC)组织中的表达并比较两者表达的相关性及分析与肿瘤临床特征的关系。方法采用免疫组化方法检测经活检确诊为NPC的患者55例及鼻咽炎组织30例中VEGF、TrKB蛋白表达水平,比较二两者表达差异。根据患者年龄和性别、肿瘤临床分型、原发灶大小、淋巴结转移和有无远处转移将病例分组,应用双变量相关Pearson检验分析研究VEGF、TrKB表达与肿瘤临床病理特征之间的关系。结果①VEGF蛋白于肿瘤细胞浆及血管内皮细胞浆中均表达,TrKB蛋白阳性表达绝大多数位于细胞浆内。在55例NPC患者中,45例TrKB阳性表达,阳性表达率为81.8%;47例VEGF阳性表达,阳性表达率为85.4%。30例鼻咽炎中,只有3例TrKB阳性表达,阳性表达率为10.0%;5例VEGF阳性表达,阳性表达率为16.7%。肿瘤组织中TrKB、VEGF阳性表达率明显高于鼻咽炎组织（P<0.05）,NPC组织中VEGF与TrKB表达有明显相关性（R=0.716,P<0.01）;②VEGF蛋白表达与NPC的临床分期(P=0.002)、肿瘤的直径大小(P=0.009)及有无淋巴结转移(P=0.001)明显相关,与患者性别、年龄、有无远处转移无关（P＞0.05）。TrKB蛋白表达与临床分期(P=0.00)、肿瘤的直径大小(P=0.00)、有无淋巴结转移（P=0.006）明显相关,与患者性别、年龄、有无远处转移无关（P＞0.05）。结论VEGF、TrKB在NPC组织中表达增加,可能与NPC的发生发展有关。TrKB可能与NPC血管形成有关。 （